Amino acid dipepetide frequency for Bacillus azotoformans LMG 9581

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.358AlaAla: 5.358 ± 0.098	0.594AlaCys: 0.594 ± 0.026	3.169AlaAsp: 3.169 ± 0.051	4.579AlaGlu: 4.579 ± 0.065	3.0AlaPhe: 3.0 ± 0.059	5.021AlaGly: 5.021 ± 0.075	1.166AlaHis: 1.166 ± 0.036	6.011AlaIle: 6.011 ± 0.075	4.815AlaLys: 4.815 ± 0.079	6.736AlaLeu: 6.736 ± 0.076	1.941AlaMet: 1.941 ± 0.044	2.946AlaAsn: 2.946 ± 0.057	2.001AlaPro: 2.001 ± 0.041	2.103AlaGln: 2.103 ± 0.047	2.393AlaArg: 2.393 ± 0.045	3.78AlaSer: 3.78 ± 0.06	3.457AlaThr: 3.457 ± 0.065	5.052AlaVal: 5.052 ± 0.071	0.561AlaTrp: 0.561 ± 0.028	2.127AlaTyr: 2.127 ± 0.045	0.0AlaXaa: 0.0 ± 0.0
Cys	0.44CysAla: 0.44 ± 0.02	0.105CysCys: 0.105 ± 0.009	0.419CysAsp: 0.419 ± 0.02	0.495CysGlu: 0.495 ± 0.021	0.343CysPhe: 0.343 ± 0.018	0.713CysGly: 0.713 ± 0.026	0.222CysHis: 0.222 ± 0.014	0.574CysIle: 0.574 ± 0.023	0.43CysLys: 0.43 ± 0.022	0.692CysLeu: 0.692 ± 0.025	0.201CysMet: 0.201 ± 0.017	0.364CysAsn: 0.364 ± 0.018	0.404CysPro: 0.404 ± 0.018	0.254CysGln: 0.254 ± 0.016	0.302CysArg: 0.302 ± 0.019	0.534CysSer: 0.534 ± 0.025	0.418CysThr: 0.418 ± 0.019	0.404CysVal: 0.404 ± 0.019	0.075CysTrp: 0.075 ± 0.007	0.279CysTyr: 0.279 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	3.073AspAla: 3.073 ± 0.055	0.418AspCys: 0.418 ± 0.02	2.612AspAsp: 2.612 ± 0.053	4.432AspGlu: 4.432 ± 0.058	2.439AspPhe: 2.439 ± 0.052	3.355AspGly: 3.355 ± 0.062	1.155AspHis: 1.155 ± 0.035	4.353AspIle: 4.353 ± 0.07	3.248AspLys: 3.248 ± 0.05	5.095AspLeu: 5.095 ± 0.065	1.256AspMet: 1.256 ± 0.03	1.955AspAsn: 1.955 ± 0.04	1.955AspPro: 1.955 ± 0.039	2.041AspGln: 2.041 ± 0.041	2.233AspArg: 2.233 ± 0.046	2.585AspSer: 2.585 ± 0.057	2.236AspThr: 2.236 ± 0.041	3.662AspVal: 3.662 ± 0.058	0.652AspTrp: 0.652 ± 0.023	2.126AspTyr: 2.126 ± 0.043	0.0AspXaa: 0.0 ± 0.0
Glu	5.025GluAla: 5.025 ± 0.075	0.388GluCys: 0.388 ± 0.019	3.743GluAsp: 3.743 ± 0.066	6.544GluGlu: 6.544 ± 0.09	2.76GluPhe: 2.76 ± 0.05	4.217GluGly: 4.217 ± 0.063	1.383GluHis: 1.383 ± 0.033	6.419GluIle: 6.419 ± 0.088	7.021GluLys: 7.021 ± 0.083	7.105GluLeu: 7.105 ± 0.081	2.284GluMet: 2.284 ± 0.043	4.082GluAsn: 4.082 ± 0.059	1.926GluPro: 1.926 ± 0.041	3.386GluGln: 3.386 ± 0.064	3.276GluArg: 3.276 ± 0.064	3.391GluSer: 3.391 ± 0.06	3.725GluThr: 3.725 ± 0.054	5.179GluVal: 5.179 ± 0.08	0.791GluTrp: 0.791 ± 0.025	2.224GluTyr: 2.224 ± 0.046	0.0GluXaa: 0.0 ± 0.0
Phe	2.934PheAla: 2.934 ± 0.061	0.375PheCys: 0.375 ± 0.022	2.445PheAsp: 2.445 ± 0.05	3.052PheGlu: 3.052 ± 0.054	2.286PhePhe: 2.286 ± 0.052	3.254PheGly: 3.254 ± 0.062	0.864PheHis: 0.864 ± 0.029	4.12PheIle: 4.12 ± 0.073	2.621PheLys: 2.621 ± 0.057	4.767PheLeu: 4.767 ± 0.08	1.154PheMet: 1.154 ± 0.031	2.096PheAsn: 2.096 ± 0.044	1.701PhePro: 1.701 ± 0.039	1.539PheGln: 1.539 ± 0.038	1.534PheArg: 1.534 ± 0.036	3.337PheSer: 3.337 ± 0.056	2.566PheThr: 2.566 ± 0.049	3.103PheVal: 3.103 ± 0.047	0.453PheTrp: 0.453 ± 0.022	1.725PheTyr: 1.725 ± 0.04	0.0PheXaa: 0.0 ± 0.0
Gly	4.714GlyAla: 4.714 ± 0.073	0.674GlyCys: 0.674 ± 0.027	3.132GlyAsp: 3.132 ± 0.05	4.471GlyGlu: 4.471 ± 0.062	3.395GlyPhe: 3.395 ± 0.06	4.639GlyGly: 4.639 ± 0.089	1.347GlyHis: 1.347 ± 0.033	6.139GlyIle: 6.139 ± 0.079	5.052GlyLys: 5.052 ± 0.07	6.507GlyLeu: 6.507 ± 0.075	2.118GlyMet: 2.118 ± 0.044	2.915GlyAsn: 2.915 ± 0.052	1.741GlyPro: 1.741 ± 0.042	2.239GlyGln: 2.239 ± 0.039	2.573GlyArg: 2.573 ± 0.061	3.724GlySer: 3.724 ± 0.058	4.122GlyThr: 4.122 ± 0.07	4.887GlyVal: 4.887 ± 0.063	0.734GlyTrp: 0.734 ± 0.024	2.707GlyTyr: 2.707 ± 0.049	0.0GlyXaa: 0.0 ± 0.0
His	1.129HisAla: 1.129 ± 0.032	0.208HisCys: 0.208 ± 0.014	0.964HisAsp: 0.964 ± 0.031	1.357HisGlu: 1.357 ± 0.035	1.037HisPhe: 1.037 ± 0.029	1.354HisGly: 1.354 ± 0.041	0.603HisHis: 0.603 ± 0.029	1.465HisIle: 1.465 ± 0.037	0.968HisLys: 0.968 ± 0.026	1.96HisLeu: 1.96 ± 0.048	0.437HisMet: 0.437 ± 0.019	0.816HisAsn: 0.816 ± 0.03	1.046HisPro: 1.046 ± 0.033	0.738HisGln: 0.738 ± 0.028	0.823HisArg: 0.823 ± 0.025	1.19HisSer: 1.19 ± 0.03	0.959HisThr: 0.959 ± 0.026	1.292HisVal: 1.292 ± 0.034	0.206HisTrp: 0.206 ± 0.014	0.838HisTyr: 0.838 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	5.975IleAla: 5.975 ± 0.086	0.699IleCys: 0.699 ± 0.027	4.721IleAsp: 4.721 ± 0.062	6.509IleGlu: 6.509 ± 0.08	3.578IlePhe: 3.578 ± 0.069	6.222IleGly: 6.222 ± 0.071	1.664IleHis: 1.664 ± 0.044	6.883IleIle: 6.883 ± 0.095	5.623IleLys: 5.623 ± 0.062	7.609IleLeu: 7.609 ± 0.094	1.936IleMet: 1.936 ± 0.043	4.117IleAsn: 4.117 ± 0.06	3.456IlePro: 3.456 ± 0.059	2.955IleGln: 2.955 ± 0.051	3.15IleArg: 3.15 ± 0.057	5.454IleSer: 5.454 ± 0.08	4.768IleThr: 4.768 ± 0.068	5.935IleVal: 5.935 ± 0.074	0.664IleTrp: 0.664 ± 0.024	2.562IleTyr: 2.562 ± 0.047	0.0IleXaa: 0.0 ± 0.0
Lys	4.915LysAla: 4.915 ± 0.076	0.423LysCys: 0.423 ± 0.019	4.097LysAsp: 4.097 ± 0.06	6.981LysGlu: 6.981 ± 0.088	2.177LysPhe: 2.177 ± 0.048	4.565LysGly: 4.565 ± 0.064	1.221LysHis: 1.221 ± 0.03	5.626LysIle: 5.626 ± 0.071	5.983LysLys: 5.983 ± 0.08	6.212LysLeu: 6.212 ± 0.078	2.218LysMet: 2.218 ± 0.043	3.845LysAsn: 3.845 ± 0.059	2.235LysPro: 2.235 ± 0.051	3.14LysGln: 3.14 ± 0.06	3.197LysArg: 3.197 ± 0.052	3.711LysSer: 3.711 ± 0.057	3.757LysThr: 3.757 ± 0.058	5.142LysVal: 5.142 ± 0.063	0.836LysTrp: 0.836 ± 0.027	2.429LysTyr: 2.429 ± 0.045	0.0LysXaa: 0.0 ± 0.0
Leu	6.804LeuAla: 6.804 ± 0.079	0.703LeuCys: 0.703 ± 0.026	4.802LeuAsp: 4.802 ± 0.069	6.713LeuGlu: 6.713 ± 0.087	4.869LeuPhe: 4.869 ± 0.094	6.406LeuGly: 6.406 ± 0.077	1.821LeuHis: 1.821 ± 0.039	7.579LeuIle: 7.579 ± 0.089	6.978LeuLys: 6.978 ± 0.074	9.93LeuLeu: 9.93 ± 0.117	2.463LeuMet: 2.463 ± 0.046	4.538LeuAsn: 4.538 ± 0.064	3.711LeuPro: 3.711 ± 0.051	3.506LeuGln: 3.506 ± 0.06	3.495LeuArg: 3.495 ± 0.058	6.599LeuSer: 6.599 ± 0.083	5.58LeuThr: 5.58 ± 0.075	6.252LeuVal: 6.252 ± 0.084	0.746LeuTrp: 0.746 ± 0.029	3.114LeuTyr: 3.114 ± 0.064	0.0LeuXaa: 0.0 ± 0.0
Met	2.022MetAla: 2.022 ± 0.046	0.159MetCys: 0.159 ± 0.013	1.45MetAsp: 1.45 ± 0.029	1.953MetGlu: 1.953 ± 0.041	1.072MetPhe: 1.072 ± 0.03	1.831MetGly: 1.831 ± 0.045	0.41MetHis: 0.41 ± 0.019	2.183MetIle: 2.183 ± 0.047	2.43MetLys: 2.43 ± 0.044	2.4MetLeu: 2.4 ± 0.044	0.836MetMet: 0.836 ± 0.032	1.571MetAsn: 1.571 ± 0.037	1.001MetPro: 1.001 ± 0.034	0.801MetGln: 0.801 ± 0.025	1.02MetArg: 1.02 ± 0.031	1.604MetSer: 1.604 ± 0.043	1.524MetThr: 1.524 ± 0.036	1.786MetVal: 1.786 ± 0.046	0.191MetTrp: 0.191 ± 0.014	0.701MetTyr: 0.701 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	2.824AsnAla: 2.824 ± 0.044	0.363AsnCys: 0.363 ± 0.018	2.482AsnAsp: 2.482 ± 0.05	3.846AsnGlu: 3.846 ± 0.063	1.944AsnPhe: 1.944 ± 0.037	3.601AsnGly: 3.601 ± 0.057	1.101AsnHis: 1.101 ± 0.025	3.866AsnIle: 3.866 ± 0.057	3.507AsnLys: 3.507 ± 0.052	4.266AsnLeu: 4.266 ± 0.063	1.206AsnMet: 1.206 ± 0.031	2.505AsnAsn: 2.505 ± 0.052	2.237AsnPro: 2.237 ± 0.043	2.026AsnGln: 2.026 ± 0.045	2.084AsnArg: 2.084 ± 0.038	2.571AsnSer: 2.571 ± 0.045	2.371AsnThr: 2.371 ± 0.05	3.228AsnVal: 3.228 ± 0.054	0.536AsnTrp: 0.536 ± 0.022	1.66AsnTyr: 1.66 ± 0.036	0.0AsnXaa: 0.0 ± 0.0
Pro	2.135ProAla: 2.135 ± 0.041	0.243ProCys: 0.243 ± 0.016	1.941ProAsp: 1.941 ± 0.045	2.923ProGlu: 2.923 ± 0.049	1.951ProPhe: 1.951 ± 0.043	2.134ProGly: 2.134 ± 0.054	0.703ProHis: 0.703 ± 0.027	3.092ProIle: 3.092 ± 0.045	2.381ProLys: 2.381 ± 0.048	3.477ProLeu: 3.477 ± 0.056	0.79ProMet: 0.79 ± 0.027	1.759ProAsn: 1.759 ± 0.043	1.035ProPro: 1.035 ± 0.035	1.053ProGln: 1.053 ± 0.034	1.079ProArg: 1.079 ± 0.032	2.225ProSer: 2.225 ± 0.047	2.139ProThr: 2.139 ± 0.036	2.678ProVal: 2.678 ± 0.05	0.34ProTrp: 0.34 ± 0.017	1.395ProTyr: 1.395 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	2.57GlnAla: 2.57 ± 0.047	0.223GlnCys: 0.223 ± 0.014	1.544GlnAsp: 1.544 ± 0.038	2.675GlnGlu: 2.675 ± 0.047	1.718GlnPhe: 1.718 ± 0.037	1.916GlnGly: 1.916 ± 0.037	0.648GlnHis: 0.648 ± 0.025	2.988GlnIle: 2.988 ± 0.048	2.854GlnLys: 2.854 ± 0.057	3.855GlnLeu: 3.855 ± 0.059	1.132GlnMet: 1.132 ± 0.034	1.782GlnAsn: 1.782 ± 0.043	1.176GlnPro: 1.176 ± 0.033	1.713GlnGln: 1.713 ± 0.053	1.366GlnArg: 1.366 ± 0.038	2.094GlnSer: 2.094 ± 0.039	1.989GlnThr: 1.989 ± 0.046	2.391GlnVal: 2.391 ± 0.051	0.363GlnTrp: 0.363 ± 0.017	1.4GlnTyr: 1.4 ± 0.033	0.0GlnXaa: 0.0 ± 0.0
Arg	2.312ArgAla: 2.312 ± 0.048	0.26ArgCys: 0.26 ± 0.015	1.95ArgAsp: 1.95 ± 0.042	3.05ArgGlu: 3.05 ± 0.058	1.919ArgPhe: 1.919 ± 0.04	2.329ArgGly: 2.329 ± 0.048	0.74ArgHis: 0.74 ± 0.024	3.216ArgIle: 3.216 ± 0.053	3.118ArgLys: 3.118 ± 0.059	3.862ArgLeu: 3.862 ± 0.07	1.211ArgMet: 1.211 ± 0.03	1.96ArgAsn: 1.96 ± 0.042	1.289ArgPro: 1.289 ± 0.033	1.406ArgGln: 1.406 ± 0.035	1.723ArgArg: 1.723 ± 0.039	2.07ArgSer: 2.07 ± 0.044	2.028ArgThr: 2.028 ± 0.039	2.517ArgVal: 2.517 ± 0.046	0.393ArgTrp: 0.393 ± 0.017	1.453ArgTyr: 1.453 ± 0.033	0.0ArgXaa: 0.0 ± 0.0
Ser	3.496SerAla: 3.496 ± 0.059	0.45SerCys: 0.45 ± 0.021	2.711SerAsp: 2.711 ± 0.047	3.809SerGlu: 3.809 ± 0.064	3.225SerPhe: 3.225 ± 0.053	4.316SerGly: 4.316 ± 0.062	1.121SerHis: 1.121 ± 0.03	5.43SerIle: 5.43 ± 0.071	4.102SerLys: 4.102 ± 0.065	5.96SerLeu: 5.96 ± 0.079	1.633SerMet: 1.633 ± 0.041	2.752SerAsn: 2.752 ± 0.049	2.096SerPro: 2.096 ± 0.042	1.977SerGln: 1.977 ± 0.041	2.186SerArg: 2.186 ± 0.04	3.699SerSer: 3.699 ± 0.066	3.163SerThr: 3.163 ± 0.056	3.846SerVal: 3.846 ± 0.058	0.579SerTrp: 0.579 ± 0.021	2.213SerTyr: 2.213 ± 0.042	0.0SerXaa: 0.0 ± 0.0
Thr	3.73ThrAla: 3.73 ± 0.058	0.384ThrCys: 0.384 ± 0.02	2.62ThrAsp: 2.62 ± 0.052	3.517ThrGlu: 3.517 ± 0.058	2.631ThrPhe: 2.631 ± 0.046	4.107ThrGly: 4.107 ± 0.066	0.936ThrHis: 0.936 ± 0.03	4.951ThrIle: 4.951 ± 0.071	3.665ThrLys: 3.665 ± 0.062	5.131ThrLeu: 5.131 ± 0.061	1.293ThrMet: 1.293 ± 0.03	2.702ThrAsn: 2.702 ± 0.05	2.215ThrPro: 2.215 ± 0.044	1.463ThrGln: 1.463 ± 0.039	1.865ThrArg: 1.865 ± 0.037	3.171ThrSer: 3.171 ± 0.054	3.144ThrThr: 3.144 ± 0.064	4.231ThrVal: 4.231 ± 0.063	0.544ThrTrp: 0.544 ± 0.022	1.886ThrTyr: 1.886 ± 0.039	0.0ThrXaa: 0.0 ± 0.0
Val	4.875ValAla: 4.875 ± 0.071	0.574ValCys: 0.574 ± 0.023	3.649ValAsp: 3.649 ± 0.057	4.832ValGlu: 4.832 ± 0.082	3.198ValPhe: 3.198 ± 0.053	4.782ValGly: 4.782 ± 0.075	1.3ValHis: 1.3 ± 0.035	6.056ValIle: 6.056 ± 0.068	4.909ValLys: 4.909 ± 0.066	6.537ValLeu: 6.537 ± 0.084	1.706ValMet: 1.706 ± 0.039	3.337ValAsn: 3.337 ± 0.052	2.702ValPro: 2.702 ± 0.047	2.335ValGln: 2.335 ± 0.048	2.561ValArg: 2.561 ± 0.053	4.268ValSer: 4.268 ± 0.056	3.983ValThr: 3.983 ± 0.06	5.033ValVal: 5.033 ± 0.075	0.595ValTrp: 0.595 ± 0.022	2.193ValTyr: 2.193 ± 0.044	0.0ValXaa: 0.0 ± 0.0
Trp	0.556TrpAla: 0.556 ± 0.025	0.082TrpCys: 0.082 ± 0.008	0.544TrpAsp: 0.544 ± 0.022	0.666TrpGlu: 0.666 ± 0.028	0.487TrpPhe: 0.487 ± 0.022	0.66TrpGly: 0.66 ± 0.025	0.206TrpHis: 0.206 ± 0.013	0.832TrpIle: 0.832 ± 0.026	0.715TrpLys: 0.715 ± 0.026	1.086TrpLeu: 1.086 ± 0.037	0.323TrpMet: 0.323 ± 0.016	0.523TrpAsn: 0.523 ± 0.023	0.226TrpPro: 0.226 ± 0.014	0.326TrpGln: 0.326 ± 0.015	0.369TrpArg: 0.369 ± 0.018	0.578TrpSer: 0.578 ± 0.021	0.467TrpThr: 0.467 ± 0.021	0.637TrpVal: 0.637 ± 0.023	0.138TrpTrp: 0.138 ± 0.011	0.359TrpTyr: 0.359 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.922TyrAla: 1.922 ± 0.043	0.342TyrCys: 0.342 ± 0.016	1.885TyrAsp: 1.885 ± 0.037	2.465TyrGlu: 2.465 ± 0.049	1.897TyrPhe: 1.897 ± 0.044	2.407TyrGly: 2.407 ± 0.044	0.778TyrHis: 0.778 ± 0.025	2.7TyrIle: 2.7 ± 0.054	2.29TyrLys: 2.29 ± 0.052	3.42TyrLeu: 3.42 ± 0.055	0.807TyrMet: 0.807 ± 0.029	1.659TyrAsn: 1.659 ± 0.04	1.322TyrPro: 1.322 ± 0.033	1.399TyrGln: 1.399 ± 0.038	1.579TyrArg: 1.579 ± 0.038	2.177TyrSer: 2.177 ± 0.044	1.79TyrThr: 1.79 ± 0.046	2.169TyrVal: 2.169 ± 0.043	0.384TyrTrp: 0.384 ± 0.017	1.431TyrTyr: 1.431 ± 0.037	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4207 proteins (1198567 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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