Ruminococcus sp. CAG:177

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Ruminococcus; environmental samples

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1937 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6IY24|R6IY24_9FIRM Translation initiation factor IF-2 OS=Ruminococcus sp. CAG:177 OX=1262952 GN=infB PE=3 SV=1
MM1 pKa = 7.29LKK3 pKa = 10.14EE4 pKa = 3.95YY5 pKa = 10.82DD6 pKa = 3.74GLPDD10 pKa = 3.17VGSRR14 pKa = 11.84YY15 pKa = 9.7VLEE18 pKa = 4.49CRR20 pKa = 11.84CKK22 pKa = 10.67KK23 pKa = 10.17IGEE26 pKa = 4.64DD27 pKa = 3.58GSEE30 pKa = 3.71IGACEE35 pKa = 3.94DD36 pKa = 3.47TVNPVSGFIYY46 pKa = 10.65AEE48 pKa = 4.05NEE50 pKa = 4.01EE51 pKa = 4.1QAVNYY56 pKa = 10.57GIDD59 pKa = 4.1FIMKK63 pKa = 9.49YY64 pKa = 10.59AGDD67 pKa = 3.88NLLLEE72 pKa = 5.27DD73 pKa = 4.75NSILLLDD80 pKa = 5.0DD81 pKa = 4.16NRR83 pKa = 11.84PMDD86 pKa = 4.37LYY88 pKa = 11.2YY89 pKa = 10.83DD90 pKa = 4.41FSAVEE95 pKa = 4.13VGG97 pKa = 3.78

Molecular weight: 10.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6IT83|R6IT83_9FIRM Transcriptional regulator RpiR family OS=Ruminococcus sp. CAG:177 OX=1262952 GN=BN517_01292 PE=4 SV=1
MM1 pKa = 7.9KK2 pKa = 10.19DD3 pKa = 3.07SQIRR7 pKa = 11.84VCGRR11 pKa = 11.84AAQTDD16 pKa = 3.92SGAGGAVMFGCLAAAAVAGGFMTAGARR43 pKa = 11.84LLLNASAVTSALSSVSKK60 pKa = 10.65LLSPAVNAAACILLTIAVSALIAPLRR86 pKa = 11.84QGMKK90 pKa = 10.1RR91 pKa = 11.84YY92 pKa = 9.18FLLGALRR99 pKa = 11.84QKK101 pKa = 10.38RR102 pKa = 11.84RR103 pKa = 11.84AAEE106 pKa = 3.77CTAAFRR112 pKa = 11.84GKK114 pKa = 9.72HH115 pKa = 5.3AFSALRR121 pKa = 11.84LHH123 pKa = 7.0IALAALNLVLWTFFLLPGAALAAGGIYY150 pKa = 9.5MLLSSNVGILTIAAVLTGSILMLISGCIFCSGARR184 pKa = 11.84QAWSAAEE191 pKa = 4.19YY192 pKa = 10.56ILAADD197 pKa = 4.15PNVSIKK203 pKa = 10.17QALRR207 pKa = 11.84QSRR210 pKa = 11.84DD211 pKa = 2.46IMRR214 pKa = 11.84GHH216 pKa = 6.63CRR218 pKa = 11.84AFARR222 pKa = 11.84FKK224 pKa = 11.11AGFILWFLSCVLILPAFFVVPYY246 pKa = 9.38YY247 pKa = 10.81GQSCAVWMTEE257 pKa = 4.05TLDD260 pKa = 3.82FKK262 pKa = 11.62NKK264 pKa = 8.79VLRR267 pKa = 11.84KK268 pKa = 9.62

Molecular weight: 28.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1937

0

1937

610976

29

3644

315.4

34.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.698 ± 0.071

1.759 ± 0.025

6.161 ± 0.046

6.719 ± 0.065

4.378 ± 0.046

7.424 ± 0.05

1.63 ± 0.022

7.136 ± 0.055

6.617 ± 0.047

8.56 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.801 ± 0.026

4.132 ± 0.033

3.579 ± 0.033

2.331 ± 0.031

4.735 ± 0.048

6.434 ± 0.05

5.286 ± 0.04

7.041 ± 0.043

0.831 ± 0.02

3.745 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski