Candidatus Accumulibacter sp. SK-02

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Betaproteobacteria incertae sedis; Candidatus Accumulibacter; unclassified Candidatus Accumulibacter

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3953 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A080M8W4|A0A080M8W4_9PROT Uncharacterized protein OS=Candidatus Accumulibacter sp. SK-02 OX=1453999 GN=AW06_002035 PE=4 SV=1
MM1 pKa = 7.6ALASWTQQQILAQLDD16 pKa = 4.22SGSHH20 pKa = 4.6WSGSTVTYY28 pKa = 10.5AFPTSSSGLYY38 pKa = 10.31GSQEE42 pKa = 3.69QAGFQGLNSVQQAAATLALQTWDD65 pKa = 4.98DD66 pKa = 5.47LITPDD71 pKa = 4.11LAKK74 pKa = 9.98VTSGSSNIEE83 pKa = 3.93FGTSSTGVEE92 pKa = 3.98YY93 pKa = 11.03AHH95 pKa = 7.37AYY97 pKa = 9.84FPTTGSVWFSRR108 pKa = 11.84AYY110 pKa = 10.42SDD112 pKa = 5.03LMNPQVGAHH121 pKa = 6.04SFLSYY126 pKa = 9.9VHH128 pKa = 6.97EE129 pKa = 4.97IGHH132 pKa = 6.83AFGLDD137 pKa = 3.1HH138 pKa = 6.75MGDD141 pKa = 4.0YY142 pKa = 11.11NGGGSWTPSSYY153 pKa = 10.58QDD155 pKa = 3.11SGVYY159 pKa = 10.31SVMSYY164 pKa = 10.11FGPNWGSGSSSGVGLVAWADD184 pKa = 3.38WVGADD189 pKa = 3.8GKK191 pKa = 10.57LYY193 pKa = 10.79SPQTPMLNDD202 pKa = 3.25IMAMQSMYY210 pKa = 10.77GAEE213 pKa = 4.17TTTRR217 pKa = 11.84TEE219 pKa = 3.69DD220 pKa = 3.0TTYY223 pKa = 11.34GFGSSDD229 pKa = 3.72FGTLSAIYY237 pKa = 9.46NFSVNLHH244 pKa = 7.08PILTIYY250 pKa = 10.78DD251 pKa = 3.71SAGTDD256 pKa = 3.54TLNLSGWSTPSLIDD270 pKa = 3.96LAPGAYY276 pKa = 9.65SSCNSMTYY284 pKa = 10.46NIAIAYY290 pKa = 6.18TCNIEE295 pKa = 4.0NAIGGAGADD304 pKa = 3.83TITGNSLNNYY314 pKa = 9.45LDD316 pKa = 3.99GGSGNDD322 pKa = 3.71SLTGGAGDD330 pKa = 3.87DD331 pKa = 3.92TLVAGAGNDD340 pKa = 3.62ALDD343 pKa = 4.08GGGGNDD349 pKa = 3.02TVVFAGTFSEE359 pKa = 4.88YY360 pKa = 10.31TFSYY364 pKa = 9.99TINQGFTFASSGSGSDD380 pKa = 3.48LIKK383 pKa = 10.89NVEE386 pKa = 4.11TFVFSNVTKK395 pKa = 10.61SASEE399 pKa = 4.13LSADD403 pKa = 4.02ASSDD407 pKa = 3.65PDD409 pKa = 3.85PGSVQVSIAAGAASIAEE426 pKa = 4.41GNSGSTAYY434 pKa = 10.39VFNVSLSKK442 pKa = 10.9ASEE445 pKa = 4.15TTQTVDD451 pKa = 2.39WRR453 pKa = 11.84LDD455 pKa = 3.41GSGASPATSSDD466 pKa = 3.36FTGATSGVVTFQPGEE481 pKa = 4.1TTKK484 pKa = 10.24TIQVLVVGDD493 pKa = 3.79TVVEE497 pKa = 4.07SDD499 pKa = 3.72EE500 pKa = 4.83GFTLSLSNPSSGLVLGTASAAGTIRR525 pKa = 11.84NDD527 pKa = 3.61DD528 pKa = 3.61VTAVPDD534 pKa = 4.69DD535 pKa = 3.97YY536 pKa = 11.84PMTTNTRR543 pKa = 11.84GAITVGGAAVSGVIEE558 pKa = 4.11QASDD562 pKa = 3.24GDD564 pKa = 4.2LFKK567 pKa = 11.02VTLKK571 pKa = 11.03ANNSYY576 pKa = 10.14IFKK579 pKa = 9.95LARR582 pKa = 11.84VSGSLDD588 pKa = 3.41PYY590 pKa = 11.28LEE592 pKa = 4.86LYY594 pKa = 10.17SSKK597 pKa = 10.65LAVITQNDD605 pKa = 3.58NAGLSTTDD613 pKa = 2.93SQIIYY618 pKa = 8.98TPTAGGTYY626 pKa = 10.35YY627 pKa = 11.05LAAKK631 pKa = 10.2DD632 pKa = 3.99RR633 pKa = 11.84AQSTGSYY640 pKa = 9.73LISAVPCSGLTIEE653 pKa = 6.01GDD655 pKa = 3.45DD656 pKa = 3.79TVNVLTGTAGNDD668 pKa = 3.17SLYY671 pKa = 11.28GLGGNDD677 pKa = 3.37TLNAGMGNDD686 pKa = 4.4LLDD689 pKa = 4.41GGAGMDD695 pKa = 3.55QLAGGKK701 pKa = 10.19GDD703 pKa = 5.79DD704 pKa = 4.08IYY706 pKa = 11.63LVDD709 pKa = 3.78NVSDD713 pKa = 3.91VVIEE717 pKa = 4.15SSAADD722 pKa = 3.41GKK724 pKa = 10.81DD725 pKa = 3.25LVKK728 pKa = 11.04ASVSWTLGANVEE740 pKa = 4.23KK741 pKa = 11.27LEE743 pKa = 4.19LTGTANLMGTGNTLANIITGNSGNNVLNGLGGVDD777 pKa = 3.73SLNGGEE783 pKa = 5.15GSDD786 pKa = 4.15LYY788 pKa = 11.55LFGLPAEE795 pKa = 4.62HH796 pKa = 6.99SSAEE800 pKa = 4.03IADD803 pKa = 3.94TGSTGVDD810 pKa = 3.24EE811 pKa = 4.9VRR813 pKa = 11.84FTATGAGTLKK823 pKa = 10.64LYY825 pKa = 10.96AGDD828 pKa = 3.64TGIEE832 pKa = 3.83RR833 pKa = 11.84VVIGTGTDD841 pKa = 3.05AVANSEE847 pKa = 4.17GTVALNVDD855 pKa = 4.4ASPLSNAITMIGNAGANILTGTAFADD881 pKa = 3.7QMDD884 pKa = 4.52GGSGNDD890 pKa = 4.11LLNGQAGIDD899 pKa = 3.77LMDD902 pKa = 4.36GEE904 pKa = 4.99EE905 pKa = 4.51GSDD908 pKa = 2.93IYY910 pKa = 11.28LIGLAAAHH918 pKa = 6.0TAAEE922 pKa = 4.24IADD925 pKa = 4.01TGSSGVDD932 pKa = 3.19EE933 pKa = 4.6VRR935 pKa = 11.84FAATTASTLTLFAGDD950 pKa = 3.53TGIEE954 pKa = 3.89RR955 pKa = 11.84VVLGTGTGAVATSTGSAALNADD977 pKa = 3.18ASALVNAITLVGNAGANILTGTEE1000 pKa = 3.59FADD1003 pKa = 4.89RR1004 pKa = 11.84IEE1006 pKa = 4.72GGGGADD1012 pKa = 3.3QLFGGSGNDD1021 pKa = 3.48TLFGGLGNDD1030 pKa = 3.48TLTGGEE1036 pKa = 4.41GEE1038 pKa = 4.39DD1039 pKa = 3.34HH1040 pKa = 6.82FVLNTAANAANNRR1053 pKa = 11.84DD1054 pKa = 3.51VLVDD1058 pKa = 4.1FEE1060 pKa = 4.93SGADD1064 pKa = 3.73HH1065 pKa = 7.33IEE1067 pKa = 3.98LSLLVFKK1074 pKa = 11.11GLGKK1078 pKa = 7.36TTGVLGEE1085 pKa = 4.06AQFWAAADD1093 pKa = 3.61AVKK1096 pKa = 10.64GHH1098 pKa = 7.01DD1099 pKa = 3.36ADD1101 pKa = 5.4DD1102 pKa = 3.46RR1103 pKa = 11.84VIYY1106 pKa = 8.32NTSTGSLYY1114 pKa = 10.94YY1115 pKa = 10.66DD1116 pKa = 3.89ADD1118 pKa = 3.97GSGKK1122 pKa = 10.06GAAVEE1127 pKa = 4.0IAVLGLSTHH1136 pKa = 6.56PTLEE1140 pKa = 4.51HH1141 pKa = 7.09GDD1143 pKa = 3.72FLLII1147 pKa = 4.64

Molecular weight: 116.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A080MKE2|A0A080MKE2_9PROT Nitrite reductase [NAD(P)H] OS=Candidatus Accumulibacter sp. SK-02 OX=1453999 GN=nasD PE=4 SV=1
MM1 pKa = 7.61IGVLTSTPLAVALTVTLAGRR21 pKa = 11.84VWTVPSAGAVRR32 pKa = 11.84LTVGGVLPPFTVIVRR47 pKa = 11.84AALVVTPPALSVARR61 pKa = 11.84AVRR64 pKa = 11.84GWLPAGTLFQVKK76 pKa = 7.2VV77 pKa = 3.37

Molecular weight: 7.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3953

0

3953

1291537

29

3115

326.7

35.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.845 ± 0.052

1.109 ± 0.014

5.323 ± 0.032

5.705 ± 0.041

3.641 ± 0.024

7.904 ± 0.045

2.304 ± 0.021

4.922 ± 0.03

3.076 ± 0.03

11.356 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.222 ± 0.018

2.688 ± 0.025

5.082 ± 0.03

3.894 ± 0.026

7.46 ± 0.046

5.645 ± 0.034

4.923 ± 0.039

7.165 ± 0.034

1.418 ± 0.017

2.315 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski