Amino acid dipepetide frequency for Planktothrix tepida PCC 9214

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.95AlaAla: 4.95 ± 0.067	0.756AlaCys: 0.756 ± 0.021	3.401AlaAsp: 3.401 ± 0.044	4.902AlaGlu: 4.902 ± 0.056	2.604AlaPhe: 2.604 ± 0.043	4.449AlaGly: 4.449 ± 0.064	1.107AlaHis: 1.107 ± 0.025	6.191AlaIle: 6.191 ± 0.063	3.741AlaLys: 3.741 ± 0.05	7.898AlaLeu: 7.898 ± 0.075	1.363AlaMet: 1.363 ± 0.029	3.139AlaAsn: 3.139 ± 0.065	2.231AlaPro: 2.231 ± 0.038	3.695AlaGln: 3.695 ± 0.053	2.748AlaArg: 2.748 ± 0.043	4.126AlaSer: 4.126 ± 0.05	4.289AlaThr: 4.289 ± 0.059	4.599AlaVal: 4.599 ± 0.045	0.866AlaTrp: 0.866 ± 0.021	2.165AlaTyr: 2.165 ± 0.048	0.006AlaXaa: 0.006 ± 0.002
Cys	0.511CysAla: 0.511 ± 0.017	0.182CysCys: 0.182 ± 0.01	0.545CysAsp: 0.545 ± 0.018	0.526CysGlu: 0.526 ± 0.017	0.476CysPhe: 0.476 ± 0.016	0.757CysGly: 0.757 ± 0.021	0.317CysHis: 0.317 ± 0.013	0.594CysIle: 0.594 ± 0.018	0.373CysLys: 0.373 ± 0.014	1.22CysLeu: 1.22 ± 0.032	0.15CysMet: 0.15 ± 0.009	0.435CysAsn: 0.435 ± 0.016	0.553CysPro: 0.553 ± 0.017	0.695CysGln: 0.695 ± 0.022	0.503CysArg: 0.503 ± 0.015	0.667CysSer: 0.667 ± 0.02	0.456CysThr: 0.456 ± 0.015	0.547CysVal: 0.547 ± 0.017	0.15CysTrp: 0.15 ± 0.009	0.452CysTyr: 0.452 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	3.162AspAla: 3.162 ± 0.041	0.549AspCys: 0.549 ± 0.019	2.287AspAsp: 2.287 ± 0.048	3.068AspGlu: 3.068 ± 0.045	2.443AspPhe: 2.443 ± 0.038	3.104AspGly: 3.104 ± 0.051	0.957AspHis: 0.957 ± 0.026	3.645AspIle: 3.645 ± 0.045	1.987AspLys: 1.987 ± 0.033	6.068AspLeu: 6.068 ± 0.073	0.691AspMet: 0.691 ± 0.021	2.033AspAsn: 2.033 ± 0.044	2.434AspPro: 2.434 ± 0.043	2.582AspGln: 2.582 ± 0.04	2.578AspArg: 2.578 ± 0.042	3.027AspSer: 3.027 ± 0.05	2.347AspThr: 2.347 ± 0.047	2.859AspVal: 2.859 ± 0.042	0.906AspTrp: 0.906 ± 0.023	2.055AspTyr: 2.055 ± 0.039	0.003AspXaa: 0.003 ± 0.001
Glu	5.134GluAla: 5.134 ± 0.066	0.46GluCys: 0.46 ± 0.016	2.909GluAsp: 2.909 ± 0.043	4.427GluGlu: 4.427 ± 0.06	2.678GluPhe: 2.678 ± 0.038	3.352GluGly: 3.352 ± 0.052	1.034GluHis: 1.034 ± 0.025	5.398GluIle: 5.398 ± 0.057	3.815GluLys: 3.815 ± 0.049	7.28GluLeu: 7.28 ± 0.077	1.382GluMet: 1.382 ± 0.029	3.054GluAsn: 3.054 ± 0.047	2.615GluPro: 2.615 ± 0.046	4.092GluGln: 4.092 ± 0.051	3.351GluArg: 3.351 ± 0.046	3.529GluSer: 3.529 ± 0.051	4.087GluThr: 4.087 ± 0.052	3.969GluVal: 3.969 ± 0.047	0.819GluTrp: 0.819 ± 0.022	1.899GluTyr: 1.899 ± 0.03	0.002GluXaa: 0.002 ± 0.001
Phe	2.598PheAla: 2.598 ± 0.036	0.567PheCys: 0.567 ± 0.017	2.187PheAsp: 2.187 ± 0.035	2.411PheGlu: 2.411 ± 0.033	1.711PhePhe: 1.711 ± 0.034	2.592PheGly: 2.592 ± 0.05	0.699PheHis: 0.699 ± 0.022	2.749PheIle: 2.749 ± 0.041	1.989PheLys: 1.989 ± 0.037	4.427PheLeu: 4.427 ± 0.057	0.768PheMet: 0.768 ± 0.017	2.252PheAsn: 2.252 ± 0.034	2.056PhePro: 2.056 ± 0.036	2.003PheGln: 2.003 ± 0.036	1.701PheArg: 1.701 ± 0.027	3.084PheSer: 3.084 ± 0.05	2.204PheThr: 2.204 ± 0.039	2.375PheVal: 2.375 ± 0.039	0.731PheTrp: 0.731 ± 0.022	1.484PheTyr: 1.484 ± 0.026	0.002PheXaa: 0.002 ± 0.001
Gly	3.843GlyAla: 3.843 ± 0.06	0.839GlyCys: 0.839 ± 0.02	3.237GlyAsp: 3.237 ± 0.066	4.161GlyGlu: 4.161 ± 0.049	3.0GlyPhe: 3.0 ± 0.05	4.574GlyGly: 4.574 ± 0.089	1.108GlyHis: 1.108 ± 0.028	5.15GlyIle: 5.15 ± 0.059	3.924GlyLys: 3.924 ± 0.055	7.011GlyLeu: 7.011 ± 0.061	1.427GlyMet: 1.427 ± 0.032	3.325GlyAsn: 3.325 ± 0.094	1.126GlyPro: 1.126 ± 0.028	3.066GlyGln: 3.066 ± 0.048	2.821GlyArg: 2.821 ± 0.045	3.835GlySer: 3.835 ± 0.055	3.823GlyThr: 3.823 ± 0.068	4.383GlyVal: 4.383 ± 0.065	1.109GlyTrp: 1.109 ± 0.027	2.498GlyTyr: 2.498 ± 0.036	0.005GlyXaa: 0.005 ± 0.002
His	0.918HisAla: 0.918 ± 0.023	0.302HisCys: 0.302 ± 0.013	0.74HisAsp: 0.74 ± 0.024	0.93HisGlu: 0.93 ± 0.023	0.863HisPhe: 0.863 ± 0.023	0.961HisGly: 0.961 ± 0.023	0.696HisHis: 0.696 ± 0.023	1.037HisIle: 1.037 ± 0.021	0.72HisLys: 0.72 ± 0.022	2.501HisLeu: 2.501 ± 0.044	0.161HisMet: 0.161 ± 0.01	0.711HisAsn: 0.711 ± 0.019	1.463HisPro: 1.463 ± 0.032	1.483HisGln: 1.483 ± 0.035	1.094HisArg: 1.094 ± 0.025	1.18HisSer: 1.18 ± 0.026	0.825HisThr: 0.825 ± 0.021	0.683HisVal: 0.683 ± 0.02	0.379HisTrp: 0.379 ± 0.015	0.752HisTyr: 0.752 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	6.128IleAla: 6.128 ± 0.059	0.74IleCys: 0.74 ± 0.022	3.802IleAsp: 3.802 ± 0.049	4.804IleGlu: 4.804 ± 0.062	2.765IlePhe: 2.765 ± 0.045	4.344IleGly: 4.344 ± 0.064	1.368IleHis: 1.368 ± 0.026	4.729IleIle: 4.729 ± 0.064	3.622IleLys: 3.622 ± 0.052	8.045IleLeu: 8.045 ± 0.079	0.902IleMet: 0.902 ± 0.021	3.595IleAsn: 3.595 ± 0.052	4.056IlePro: 4.056 ± 0.053	3.758IleGln: 3.758 ± 0.047	3.178IleArg: 3.178 ± 0.042	4.918IleSer: 4.918 ± 0.047	4.185IleThr: 4.185 ± 0.06	4.169IleVal: 4.169 ± 0.056	0.921IleTrp: 0.921 ± 0.025	2.254IleTyr: 2.254 ± 0.038	0.003IleXaa: 0.003 ± 0.001
Lys	3.884LysAla: 3.884 ± 0.055	0.32LysCys: 0.32 ± 0.015	2.22LysAsp: 2.22 ± 0.035	2.724LysGlu: 2.724 ± 0.045	1.857LysPhe: 1.857 ± 0.033	2.817LysGly: 2.817 ± 0.043	0.79LysHis: 0.79 ± 0.02	4.225LysIle: 4.225 ± 0.05	2.539LysLys: 2.539 ± 0.051	5.58LysLeu: 5.58 ± 0.067	0.964LysMet: 0.964 ± 0.023	2.552LysAsn: 2.552 ± 0.043	2.787LysPro: 2.787 ± 0.045	2.971LysGln: 2.971 ± 0.046	2.405LysArg: 2.405 ± 0.041	3.119LysSer: 3.119 ± 0.051	3.57LysThr: 3.57 ± 0.054	3.033LysVal: 3.033 ± 0.046	0.517LysTrp: 0.517 ± 0.016	1.389LysTyr: 1.389 ± 0.03	0.004LysXaa: 0.004 ± 0.001
Leu	8.571LeuAla: 8.571 ± 0.071	0.998LeuCys: 0.998 ± 0.02	5.538LeuAsp: 5.538 ± 0.056	8.243LeuGlu: 8.243 ± 0.087	4.046LeuPhe: 4.046 ± 0.054	7.81LeuGly: 7.81 ± 0.074	1.778LeuHis: 1.778 ± 0.038	7.975LeuIle: 7.975 ± 0.08	6.769LeuLys: 6.769 ± 0.077	11.099LeuLeu: 11.099 ± 0.128	2.397LeuMet: 2.397 ± 0.037	5.651LeuAsn: 5.651 ± 0.058	5.341LeuPro: 5.341 ± 0.06	5.637LeuGln: 5.637 ± 0.065	5.012LeuArg: 5.012 ± 0.062	7.892LeuSer: 7.892 ± 0.067	7.181LeuThr: 7.181 ± 0.068	6.471LeuVal: 6.471 ± 0.059	1.514LeuTrp: 1.514 ± 0.038	2.929LeuTyr: 2.929 ± 0.043	0.004LeuXaa: 0.004 ± 0.001
Met	1.642MetAla: 1.642 ± 0.031	0.119MetCys: 0.119 ± 0.007	0.758MetAsp: 0.758 ± 0.021	1.017MetGlu: 1.017 ± 0.023	0.558MetPhe: 0.558 ± 0.016	1.539MetGly: 1.539 ± 0.033	0.198MetHis: 0.198 ± 0.011	1.37MetIle: 1.37 ± 0.024	1.008MetLys: 1.008 ± 0.023	1.664MetLeu: 1.664 ± 0.029	0.48MetMet: 0.48 ± 0.017	1.001MetAsn: 1.001 ± 0.026	0.808MetPro: 0.808 ± 0.021	0.845MetGln: 0.845 ± 0.021	0.835MetArg: 0.835 ± 0.022	1.319MetSer: 1.319 ± 0.028	1.235MetThr: 1.235 ± 0.026	1.248MetVal: 1.248 ± 0.028	0.139MetTrp: 0.139 ± 0.009	0.346MetTyr: 0.346 ± 0.015	0.001MetXaa: 0.001 ± 0.001
Asn	2.703AsnAla: 2.703 ± 0.039	0.545AsnCys: 0.545 ± 0.016	1.965AsnAsp: 1.965 ± 0.061	1.897AsnGlu: 1.897 ± 0.036	2.144AsnPhe: 2.144 ± 0.039	2.714AsnGly: 2.714 ± 0.056	1.074AsnHis: 1.074 ± 0.029	3.131AsnIle: 3.131 ± 0.054	1.742AsnLys: 1.742 ± 0.036	6.349AsnLeu: 6.349 ± 0.09	0.636AsnMet: 0.636 ± 0.018	2.316AsnAsn: 2.316 ± 0.055	3.332AsnPro: 3.332 ± 0.044	3.48AsnGln: 3.48 ± 0.051	2.493AsnArg: 2.493 ± 0.039	3.576AsnSer: 3.576 ± 0.06	2.492AsnThr: 2.492 ± 0.049	2.126AsnVal: 2.126 ± 0.041	0.855AsnTrp: 0.855 ± 0.023	1.699AsnTyr: 1.699 ± 0.032	0.005AsnXaa: 0.005 ± 0.002
Pro	2.622ProAla: 2.622 ± 0.041	0.371ProCys: 0.371 ± 0.015	3.154ProAsp: 3.154 ± 0.038	4.285ProGlu: 4.285 ± 0.048	1.838ProPhe: 1.838 ± 0.033	2.803ProGly: 2.803 ± 0.043	0.94ProHis: 0.94 ± 0.026	3.318ProIle: 3.318 ± 0.046	2.299ProLys: 2.299 ± 0.039	5.046ProLeu: 5.046 ± 0.059	0.756ProMet: 0.756 ± 0.021	2.557ProAsn: 2.557 ± 0.043	2.472ProPro: 2.472 ± 0.051	2.856ProGln: 2.856 ± 0.041	1.643ProArg: 1.643 ± 0.031	3.097ProSer: 3.097 ± 0.049	2.985ProThr: 2.985 ± 0.067	3.144ProVal: 3.144 ± 0.039	0.596ProTrp: 0.596 ± 0.018	1.366ProTyr: 1.366 ± 0.028	0.003ProXaa: 0.003 ± 0.001
Gln	4.471GlnAla: 4.471 ± 0.061	0.429GlnCys: 0.429 ± 0.017	2.477GlnAsp: 2.477 ± 0.044	3.922GlnGlu: 3.922 ± 0.058	2.214GlnPhe: 2.214 ± 0.034	3.772GlnGly: 3.772 ± 0.046	0.961GlnHis: 0.961 ± 0.025	3.97GlnIle: 3.97 ± 0.056	3.227GlnLys: 3.227 ± 0.044	6.617GlnLeu: 6.617 ± 0.073	1.093GlnMet: 1.093 ± 0.027	2.504GlnAsn: 2.504 ± 0.039	2.831GlnPro: 2.831 ± 0.045	4.29GlnGln: 4.29 ± 0.073	2.736GlnArg: 2.736 ± 0.041	3.272GlnSer: 3.272 ± 0.047	3.309GlnThr: 3.309 ± 0.047	3.736GlnVal: 3.736 ± 0.046	0.865GlnTrp: 0.865 ± 0.027	1.569GlnTyr: 1.569 ± 0.028	0.006GlnXaa: 0.006 ± 0.002
Arg	2.546ArgAla: 2.546 ± 0.039	0.488ArgCys: 0.488 ± 0.015	2.252ArgAsp: 2.252 ± 0.03	3.071ArgGlu: 3.071 ± 0.047	2.152ArgPhe: 2.152 ± 0.036	2.607ArgGly: 2.607 ± 0.042	0.944ArgHis: 0.944 ± 0.022	3.297ArgIle: 3.297 ± 0.041	2.194ArgLys: 2.194 ± 0.038	5.534ArgLeu: 5.534 ± 0.055	0.979ArgMet: 0.979 ± 0.025	2.035ArgAsn: 2.035 ± 0.036	1.807ArgPro: 1.807 ± 0.033	3.006ArgGln: 3.006 ± 0.044	2.528ArgArg: 2.528 ± 0.042	2.908ArgSer: 2.908 ± 0.049	2.33ArgThr: 2.33 ± 0.042	2.976ArgVal: 2.976 ± 0.047	0.771ArgTrp: 0.771 ± 0.021	1.828ArgTyr: 1.828 ± 0.032	0.002ArgXaa: 0.002 ± 0.001
Ser	3.793SerAla: 3.793 ± 0.045	0.676SerCys: 0.676 ± 0.018	3.31SerAsp: 3.31 ± 0.05	4.143SerGlu: 4.143 ± 0.048	2.532SerPhe: 2.532 ± 0.044	4.462SerGly: 4.462 ± 0.064	1.327SerHis: 1.327 ± 0.03	4.204SerIle: 4.204 ± 0.052	2.758SerLys: 2.758 ± 0.051	7.658SerLeu: 7.658 ± 0.07	1.154SerMet: 1.154 ± 0.025	2.847SerAsn: 2.847 ± 0.052	3.846SerPro: 3.846 ± 0.053	4.028SerGln: 4.028 ± 0.054	2.932SerArg: 2.932 ± 0.046	4.795SerSer: 4.795 ± 0.065	3.372SerThr: 3.372 ± 0.045	3.945SerVal: 3.945 ± 0.054	0.923SerTrp: 0.923 ± 0.026	2.008SerTyr: 2.008 ± 0.034	0.005SerXaa: 0.005 ± 0.002
Thr	4.41ThrAla: 4.41 ± 0.062	0.515ThrCys: 0.515 ± 0.017	2.783ThrAsp: 2.783 ± 0.044	3.73ThrGlu: 3.73 ± 0.051	2.124ThrPhe: 2.124 ± 0.044	4.314ThrGly: 4.314 ± 0.077	1.128ThrHis: 1.128 ± 0.026	3.92ThrIle: 3.92 ± 0.053	2.078ThrLys: 2.078 ± 0.034	7.14ThrLeu: 7.14 ± 0.078	0.73ThrMet: 0.73 ± 0.02	2.235ThrAsn: 2.235 ± 0.042	3.749ThrPro: 3.749 ± 0.069	3.328ThrGln: 3.328 ± 0.048	2.243ThrArg: 2.243 ± 0.043	3.377ThrSer: 3.377 ± 0.044	3.449ThrThr: 3.449 ± 0.061	4.233ThrVal: 4.233 ± 0.061	0.755ThrTrp: 0.755 ± 0.024	1.614ThrTyr: 1.614 ± 0.035	0.006ThrXaa: 0.006 ± 0.002
Val	4.766ValAla: 4.766 ± 0.056	0.65ValCys: 0.65 ± 0.021	3.047ValAsp: 3.047 ± 0.045	4.272ValGlu: 4.272 ± 0.054	2.466ValPhe: 2.466 ± 0.036	4.179ValGly: 4.179 ± 0.07	0.931ValHis: 0.931 ± 0.026	4.498ValIle: 4.498 ± 0.052	3.351ValLys: 3.351 ± 0.049	6.218ValLeu: 6.218 ± 0.063	1.344ValMet: 1.344 ± 0.028	2.959ValAsn: 2.959 ± 0.048	2.551ValPro: 2.551 ± 0.035	2.807ValGln: 2.807 ± 0.041	2.74ValArg: 2.74 ± 0.04	4.031ValSer: 4.031 ± 0.052	3.456ValThr: 3.456 ± 0.062	4.025ValVal: 4.025 ± 0.06	0.824ValTrp: 0.824 ± 0.022	1.83ValTyr: 1.83 ± 0.032	0.005ValXaa: 0.005 ± 0.002
Trp	0.79TrpAla: 0.79 ± 0.027	0.156TrpCys: 0.156 ± 0.009	0.698TrpAsp: 0.698 ± 0.019	0.984TrpGlu: 0.984 ± 0.025	0.622TrpPhe: 0.622 ± 0.021	1.039TrpGly: 1.039 ± 0.029	0.289TrpHis: 0.289 ± 0.013	1.003TrpIle: 1.003 ± 0.027	0.827TrpLys: 0.827 ± 0.022	1.78TrpLeu: 1.78 ± 0.037	0.34TrpMet: 0.34 ± 0.014	0.663TrpAsn: 0.663 ± 0.023	0.334TrpPro: 0.334 ± 0.014	1.068TrpGln: 1.068 ± 0.027	0.702TrpArg: 0.702 ± 0.022	0.839TrpSer: 0.839 ± 0.026	0.664TrpThr: 0.664 ± 0.02	1.04TrpVal: 1.04 ± 0.025	0.223TrpTrp: 0.223 ± 0.011	0.405TrpTyr: 0.405 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.78TyrAla: 1.78 ± 0.033	0.445TyrCys: 0.445 ± 0.015	1.474TyrAsp: 1.474 ± 0.029	1.768TyrGlu: 1.768 ± 0.035	1.493TyrPhe: 1.493 ± 0.034	2.072TyrGly: 2.072 ± 0.038	0.754TyrHis: 0.754 ± 0.024	1.874TyrIle: 1.874 ± 0.034	1.303TyrLys: 1.303 ± 0.029	3.714TyrLeu: 3.714 ± 0.052	0.41TyrMet: 0.41 ± 0.012	1.403TyrAsn: 1.403 ± 0.032	1.754TyrPro: 1.754 ± 0.035	2.578TyrGln: 2.578 ± 0.044	2.009TyrArg: 2.009 ± 0.036	2.119TyrSer: 2.119 ± 0.037	1.599TyrThr: 1.599 ± 0.03	1.389TyrVal: 1.389 ± 0.028	0.602TyrTrp: 0.602 ± 0.018	1.244TyrTyr: 1.244 ± 0.032	0.003TyrXaa: 0.003 ± 0.001
Xaa	0.003XaaAla: 0.003 ± 0.001	0.001XaaCys: 0.001 ± 0.001	0.003XaaAsp: 0.003 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.003XaaPhe: 0.003 ± 0.001	0.004XaaGly: 0.004 ± 0.001	0.001XaaHis: 0.001 ± 0.0	0.005XaaIle: 0.005 ± 0.002	0.001XaaLys: 0.001 ± 0.001	0.009XaaLeu: 0.009 ± 0.002	0.004XaaMet: 0.004 ± 0.001	0.003XaaAsn: 0.003 ± 0.001	0.005XaaPro: 0.005 ± 0.002	0.003XaaGln: 0.003 ± 0.001	0.003XaaArg: 0.003 ± 0.001	0.008XaaSer: 0.008 ± 0.002	0.004XaaThr: 0.004 ± 0.002	0.003XaaVal: 0.003 ± 0.001	0.001XaaTrp: 0.001 ± 0.001	0.002XaaTyr: 0.002 ± 0.001	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5889 proteins (1873568 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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