Xanthomonas phage Xf109
Average proteome isoelectric point is 8.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1D6ZIU6|A0A1D6ZIU6_9VIRU DNA-Binding protein G5P OS=Xanthomonas phage Xf109 OX=1852597 GN=Xf109_2 PE=3 SV=1
MM1 pKa = 7.77 AGGLRR6 pKa = 11.84 DD7 pKa = 3.72 CLRR10 pKa = 11.84 GFRR13 pKa = 11.84 IMGWLARR20 pKa = 11.84 VFASAIARR28 pKa = 11.84 RR29 pKa = 11.84 LAYY32 pKa = 10.38 VLVAATLAWCGIGCASAQDD51 pKa = 4.37 FGTQGAAYY59 pKa = 8.25 SACMSQTAAYY69 pKa = 8.79 LASLGRR75 pKa = 11.84 GANDD79 pKa = 3.87 RR80 pKa = 11.84 NPEE83 pKa = 4.17 CNVEE87 pKa = 4.15 QGSQAYY93 pKa = 9.58 RR94 pKa = 11.84 GQFEE98 pKa = 4.21 TRR100 pKa = 11.84 DD101 pKa = 3.73 CSSCDD106 pKa = 2.74 WYY108 pKa = 10.62 RR109 pKa = 11.84 AYY111 pKa = 10.81 YY112 pKa = 10.46 GYY114 pKa = 10.51 FPWSKK119 pKa = 11.11 GCDD122 pKa = 3.37 QEE124 pKa = 4.35 PDD126 pKa = 3.69 YY127 pKa = 11.04 TGSGPWGTYY136 pKa = 9.79 VGTARR141 pKa = 11.84 NGSIGCRR148 pKa = 11.84 NGCDD152 pKa = 3.55 GVWFGNGDD160 pKa = 4.33 DD161 pKa = 3.78 TMTWNATGAICPKK174 pKa = 10.5 DD175 pKa = 3.59 PEE177 pKa = 4.47 KK178 pKa = 10.67 TCDD181 pKa = 3.58 AMSGGYY187 pKa = 8.8 GWNGYY192 pKa = 10.1 LGVCEE197 pKa = 4.44 PPPTEE202 pKa = 4.26 EE203 pKa = 4.33 CPEE206 pKa = 4.09 GQVPDD211 pKa = 4.11 GKK213 pKa = 10.9 GGCSSNKK220 pKa = 9.59 CPEE223 pKa = 3.96 GMLLQADD230 pKa = 4.98 GTCAPKK236 pKa = 10.83 KK237 pKa = 9.58 NDD239 pKa = 3.67 CPAGQIRR246 pKa = 11.84 SPSGSCLPGDD256 pKa = 4.19 GQCAAGEE263 pKa = 4.2 VRR265 pKa = 11.84 GPDD268 pKa = 3.62 GTCKK272 pKa = 10.24 KK273 pKa = 10.69 DD274 pKa = 3.18 GDD276 pKa = 4.3 GDD278 pKa = 4.23 GEE280 pKa = 4.16 PDD282 pKa = 3.55 EE283 pKa = 5.35 PGEE286 pKa = 4.14 GDD288 pKa = 2.97 KK289 pKa = 11.39 SQFSGGDD296 pKa = 3.37 SCDD299 pKa = 3.89 SPPSCSGDD307 pKa = 3.87 AIMCGQARR315 pKa = 11.84 IQWRR319 pKa = 11.84 IDD321 pKa = 3.14 CNTRR325 pKa = 11.84 RR326 pKa = 11.84 DD327 pKa = 3.65 VNITGGSCASMPVCVGEE344 pKa = 3.95 NCKK347 pKa = 10.32 ALEE350 pKa = 3.9 YY351 pKa = 10.77 SQLLMQWRR359 pKa = 11.84 AACALEE365 pKa = 4.09 KK366 pKa = 10.52 AANNSGGGTGNNADD380 pKa = 3.25 VKK382 pKa = 10.58 AIRR385 pKa = 11.84 DD386 pKa = 4.11 AITGNGTADD395 pKa = 2.97 IGADD399 pKa = 3.86 GKK401 pKa = 10.07 PADD404 pKa = 4.27 AFSDD408 pKa = 3.4 EE409 pKa = 4.41 SGYY412 pKa = 11.23 GEE414 pKa = 4.09 HH415 pKa = 7.42 GYY417 pKa = 8.04 PTGEE421 pKa = 4.5 LDD423 pKa = 3.36 THH425 pKa = 6.16 GFGYY429 pKa = 10.55 SRR431 pKa = 11.84 TCPTIPDD438 pKa = 3.49 VAVFGQTLHH447 pKa = 6.82 FDD449 pKa = 3.53 TSKK452 pKa = 9.79 FCQWMVLGGQIVLVMASLVSLRR474 pKa = 11.84 LMSQGGSAA482 pKa = 3.86
Molecular weight: 50.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.426
IPC2_protein 4.558
IPC_protein 4.52
Toseland 4.342
ProMoST 4.66
Dawson 4.495
Bjellqvist 4.647
Wikipedia 4.406
Rodwell 4.355
Grimsley 4.24
Solomon 4.495
Lehninger 4.457
Nozaki 4.596
DTASelect 4.838
Thurlkill 4.355
EMBOSS 4.418
Sillero 4.647
Patrickios 0.718
IPC_peptide 4.495
IPC2_peptide 4.635
IPC2.peptide.svr19 4.534
Protein with the highest isoelectric point:
>tr|A0A1D6ZIV1|A0A1D6ZIV1_9VIRU Uncharacterized protein OS=Xanthomonas phage Xf109 OX=1852597 GN=Xf109_11 PE=4 SV=1
MM1 pKa = 7.43 TFDD4 pKa = 2.89 TYY6 pKa = 11.55 EE7 pKa = 4.21 RR8 pKa = 11.84 VDD10 pKa = 4.05 LTGPWAGFGFQGHH23 pKa = 6.07 RR24 pKa = 11.84 FFTPEE29 pKa = 4.2 NYY31 pKa = 10.47 DD32 pKa = 3.09 IDD34 pKa = 4.44 PCGMRR39 pKa = 11.84 YY40 pKa = 8.0 WALTCAIARR49 pKa = 11.84 EE50 pKa = 4.08 WSLMMSEE57 pKa = 4.29 EE58 pKa = 4.61 RR59 pKa = 11.84 NAQSANPRR67 pKa = 11.84 TPTATRR73 pKa = 11.84 SPGSRR78 pKa = 11.84 ISEE81 pKa = 4.28 DD82 pKa = 3.26 ANVIYY87 pKa = 10.62 LRR89 pKa = 11.84 DD90 pKa = 3.66 VLWRR94 pKa = 11.84 RR95 pKa = 11.84 RR96 pKa = 11.84 EE97 pKa = 3.78 KK98 pKa = 10.64 RR99 pKa = 11.84 LSVGGNPGLADD110 pKa = 3.44 SGKK113 pKa = 10.14 RR114 pKa = 11.84 SPTGRR119 pKa = 11.84 GRR121 pKa = 11.84 RR122 pKa = 11.84 RR123 pKa = 11.84 PRR125 pKa = 11.84 RR126 pKa = 11.84 GG127 pKa = 2.87
Molecular weight: 14.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.327
IPC2_protein 9.575
IPC_protein 10.716
Toseland 10.438
ProMoST 10.452
Dawson 10.628
Bjellqvist 10.467
Wikipedia 10.935
Rodwell 10.511
Grimsley 10.701
Solomon 10.804
Lehninger 10.745
Nozaki 10.467
DTASelect 10.467
Thurlkill 10.511
EMBOSS 10.891
Sillero 10.57
Patrickios 10.175
IPC_peptide 10.789
IPC2_peptide 9.809
IPC2.peptide.svr19 8.436
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12
0
12
2202
61
482
183.5
19.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.398 ± 0.882
2.316 ± 0.929
5.086 ± 0.715
3.724 ± 0.492
3.815 ± 0.654
9.991 ± 1.204
1.635 ± 0.325
3.951 ± 0.5
4.042 ± 0.653
8.447 ± 1.513
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.543 ± 0.279
2.316 ± 0.339
4.905 ± 0.603
3.951 ± 0.623
7.039 ± 0.947
5.631 ± 0.551
5.858 ± 0.756
6.63 ± 1.124
2.861 ± 0.494
2.861 ± 0.324
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here