Klebsiella phage phiBO1E

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Slopekvirinae; Drulisvirus; Klebsiella virus BO1E

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1U8VDH7|A0A1U8VDH7_9CAUD DNA-directed RNA polymerase OS=Klebsiella phage phiBO1E OX=1555207 GN=BO1E_0039 PE=3 SV=1
MM1 pKa = 7.94DD2 pKa = 5.01KK3 pKa = 11.11VLDD6 pKa = 4.27AYY8 pKa = 9.88KK9 pKa = 10.7KK10 pKa = 9.9LVLAVGYY17 pKa = 7.11VNCSAVQGFDD27 pKa = 4.01NGDD30 pKa = 3.36QLGAVYY36 pKa = 10.53DD37 pKa = 4.29ALDD40 pKa = 4.06KK41 pKa = 11.13LAALYY46 pKa = 10.99GMDD49 pKa = 4.95LEE51 pKa = 4.79LAATAFKK58 pKa = 10.44EE59 pKa = 4.24HH60 pKa = 6.94NDD62 pKa = 3.63LAAHH66 pKa = 6.6ADD68 pKa = 3.59KK69 pKa = 11.31LRR71 pKa = 11.84GDD73 pKa = 4.14DD74 pKa = 3.47LALIRR79 pKa = 11.84VVGTLSIGLAEE90 pKa = 4.81IGSCIYY96 pKa = 10.85DD97 pKa = 3.54ADD99 pKa = 3.98QSLSTPEE106 pKa = 4.45VIGDD110 pKa = 3.57MLGTVLVLSEE120 pKa = 4.92LEE122 pKa = 4.37GKK124 pKa = 9.79

Molecular weight: 13.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1U8VF65|A0A1U8VF65_9CAUD DNA helicase OS=Klebsiella phage phiBO1E OX=1555207 GN=BO1E_0017 PE=4 SV=1
MM1 pKa = 7.92AYY3 pKa = 10.16GKK5 pKa = 10.03NPEE8 pKa = 4.15TLLMRR13 pKa = 11.84RR14 pKa = 11.84QQPTIDD20 pKa = 3.29GLARR24 pKa = 11.84EE25 pKa = 4.81HH26 pKa = 6.27SAKK29 pKa = 10.52AALRR33 pKa = 11.84QHH35 pKa = 6.17YY36 pKa = 7.59EE37 pKa = 3.71KK38 pKa = 10.5QAQRR42 pKa = 11.84LGMTLRR48 pKa = 11.84GYY50 pKa = 9.38CVRR53 pKa = 11.84FNVRR57 pKa = 11.84GVVV60 pKa = 3.11

Molecular weight: 6.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

13629

40

1232

231.0

25.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.375 ± 0.598

1.181 ± 0.186

6.156 ± 0.249

5.87 ± 0.379

3.236 ± 0.166

7.433 ± 0.263

1.842 ± 0.172

4.329 ± 0.179

4.865 ± 0.311

8.724 ± 0.257

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.928 ± 0.186

3.991 ± 0.239

3.771 ± 0.248

4.762 ± 0.361

5.965 ± 0.295

6.266 ± 0.279

5.84 ± 0.26

7.191 ± 0.248

1.379 ± 0.1

3.896 ± 0.228
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski