Cyanophage PSS2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 128 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C7F4C3|C7F4C3_9CAUD Predicted protein OS=Cyanophage PSS2 OX=658401 GN=CSPG_00074 PE=4 SV=1
MM1 pKa = 7.79EE2 pKa = 5.66IFMACANYY10 pKa = 10.54AVGFGYY16 pKa = 10.8RR17 pKa = 11.84LFLVPVSACALDD29 pKa = 4.03LSSISGGIADD39 pKa = 4.27TGAFINLGTADD50 pKa = 4.06ANVVASTYY58 pKa = 11.0NLVEE62 pKa = 4.38GATSDD67 pKa = 4.84DD68 pKa = 3.52IQGGLTAPANIVFDD82 pKa = 3.88NDD84 pKa = 3.48VVSTEE89 pKa = 4.29TVFEE93 pKa = 4.33LFGLSAATLEE103 pKa = 4.42TDD105 pKa = 3.35TGTEE109 pKa = 3.7TAATYY114 pKa = 11.01DD115 pKa = 3.92LLSEE119 pKa = 4.57GFDD122 pKa = 3.43EE123 pKa = 5.22NVPISQSWSMSLEE136 pKa = 3.85GVIKK140 pKa = 10.01NTDD143 pKa = 2.84AGYY146 pKa = 11.32KK147 pKa = 10.08LLRR150 pKa = 11.84LLNKK154 pKa = 9.89NAVSGGLYY162 pKa = 10.59AKK164 pKa = 9.92IGRR167 pKa = 11.84IGPTGTTEE175 pKa = 4.86AIYY178 pKa = 10.04GYY180 pKa = 10.71VSVNGFSEE188 pKa = 4.45ANAAKK193 pKa = 9.58TLVKK197 pKa = 10.21WSATAQGYY205 pKa = 9.81GPFNITLDD213 pKa = 3.73NAGGTNADD221 pKa = 3.5GTTT224 pKa = 3.24

Molecular weight: 23.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C7F4F2|C7F4F2_9CAUD Predicted protein OS=Cyanophage PSS2 OX=658401 GN=CSPG_00102 PE=4 SV=1
MM1 pKa = 7.34NGYY4 pKa = 8.94PKK6 pKa = 10.38RR7 pKa = 11.84IRR9 pKa = 11.84NQILALHH16 pKa = 5.53EE17 pKa = 3.7QGMAINEE24 pKa = 3.64ISRR27 pKa = 11.84RR28 pKa = 11.84LRR30 pKa = 11.84GLIDD34 pKa = 4.28RR35 pKa = 11.84DD36 pKa = 4.03TVKK39 pKa = 10.53RR40 pKa = 11.84WVAEE44 pKa = 4.04HH45 pKa = 6.67KK46 pKa = 10.45NSKK49 pKa = 10.39GSS51 pKa = 3.28

Molecular weight: 5.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

128

0

128

32021

42

2612

250.2

27.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.656 ± 0.473

1.733 ± 0.812

7.061 ± 0.507

6.596 ± 0.332

3.351 ± 0.216

8.235 ± 0.319

1.702 ± 0.155

4.747 ± 0.195

4.769 ± 0.556

7.954 ± 0.346

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.108 ± 0.201

4.475 ± 0.248

4.369 ± 0.189

3.876 ± 0.177

4.684 ± 0.296

6.895 ± 0.242

6.749 ± 0.381

6.461 ± 0.203

1.677 ± 0.154

2.901 ± 0.185
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski