Bacillus bataviensis LMG 21833

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Neobacillus; Neobacillus bataviensis

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5206 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K6DPB8|K6DPB8_9BACI Deferrochelatase/peroxidase OS=Bacillus bataviensis LMG 21833 OX=1117379 GN=BABA_07326 PE=3 SV=1
MM1 pKa = 7.34NATYY5 pKa = 10.43LIVYY9 pKa = 10.6ASMTGNTEE17 pKa = 3.9EE18 pKa = 4.11IANLIGSGIQEE29 pKa = 4.07AGGTVLIKK37 pKa = 10.64DD38 pKa = 3.49ILEE41 pKa = 4.08VDD43 pKa = 3.61VTDD46 pKa = 4.37LQNYY50 pKa = 9.79DD51 pKa = 4.51CILLGAYY58 pKa = 7.46TWGDD62 pKa = 2.89GDD64 pKa = 5.4LPDD67 pKa = 4.71EE68 pKa = 4.72FLDD71 pKa = 5.97FYY73 pKa = 11.76DD74 pKa = 5.71EE75 pKa = 4.42MYY77 pKa = 10.92QLNLSGKK84 pKa = 8.69RR85 pKa = 11.84VAAFGSCDD93 pKa = 2.9SSYY96 pKa = 9.77EE97 pKa = 4.08HH98 pKa = 7.15HH99 pKa = 6.89GGAVDD104 pKa = 4.44ILTEE108 pKa = 3.97KK109 pKa = 10.52LAEE112 pKa = 4.33LGADD116 pKa = 3.89LVHH119 pKa = 7.39DD120 pKa = 4.35GLKK123 pKa = 9.82IDD125 pKa = 4.89LSPNTAEE132 pKa = 5.54KK133 pKa = 10.03EE134 pKa = 3.95EE135 pKa = 4.22CMKK138 pKa = 10.77FGQTIVEE145 pKa = 4.42KK146 pKa = 10.79FF147 pKa = 3.13

Molecular weight: 16.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K6DP66|K6DP66_9BACI Uncharacterized protein OS=Bacillus bataviensis LMG 21833 OX=1117379 GN=BABA_07046 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.24QPNKK9 pKa = 8.16RR10 pKa = 11.84KK11 pKa = 9.54HH12 pKa = 5.99SKK14 pKa = 8.79VHH16 pKa = 5.85GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSANGRR28 pKa = 11.84KK29 pKa = 8.77VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.43GRR39 pKa = 11.84KK40 pKa = 8.78VLSAA44 pKa = 4.05

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5206

0

5206

1530599

24

2019

294.0

32.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.014 ± 0.037

0.74 ± 0.01

4.898 ± 0.025

7.153 ± 0.039

4.741 ± 0.028

7.069 ± 0.032

2.059 ± 0.018

8.021 ± 0.036

7.168 ± 0.034

9.829 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.79 ± 0.016

4.511 ± 0.024

3.704 ± 0.021

3.625 ± 0.022

3.89 ± 0.026

5.957 ± 0.021

5.372 ± 0.023

6.95 ± 0.026

1.051 ± 0.013

3.457 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski