Bromus-associated circular DNA virus 3

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus bromas1; Bromus associated gemycircularvirus 1

Average proteome isoelectric point is 7.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4U9K5|A0A0B4U9K5_9VIRU Capsid protein OS=Bromus-associated circular DNA virus 3 OX=1590156 PE=4 SV=1
MM1 pKa = 7.87PSFDD5 pKa = 3.51LHH7 pKa = 6.63CRR9 pKa = 11.84YY10 pKa = 10.41ALLTYY15 pKa = 8.75AQCGEE20 pKa = 4.33LSADD24 pKa = 3.32AVGEE28 pKa = 3.91FLQGMGFSCVVGRR41 pKa = 11.84EE42 pKa = 3.95DD43 pKa = 3.46HH44 pKa = 6.6QDD46 pKa = 3.36GGVHH50 pKa = 5.79LHH52 pKa = 6.49CFVDD56 pKa = 4.74FGRR59 pKa = 11.84KK60 pKa = 7.8RR61 pKa = 11.84RR62 pKa = 11.84FRR64 pKa = 11.84RR65 pKa = 11.84VDD67 pKa = 3.21VFDD70 pKa = 3.69VEE72 pKa = 4.89GRR74 pKa = 11.84HH75 pKa = 6.09PNISPSKK82 pKa = 7.94GTPAKK87 pKa = 10.51GYY89 pKa = 10.65DD90 pKa = 3.43YY91 pKa = 10.68AIKK94 pKa = 10.32DD95 pKa = 3.5GRR97 pKa = 11.84IEE99 pKa = 4.17YY100 pKa = 10.42CSLDD104 pKa = 3.44RR105 pKa = 11.84PGPAEE110 pKa = 3.75KK111 pKa = 10.57SGTIEE116 pKa = 3.39KK117 pKa = 9.85WSRR120 pKa = 11.84ITSASDD126 pKa = 3.68RR127 pKa = 11.84EE128 pKa = 4.58SFWDD132 pKa = 4.13LVHH135 pKa = 7.0EE136 pKa = 5.17LDD138 pKa = 4.48PKK140 pKa = 10.85SAACCHH146 pKa = 5.82TQLQKK151 pKa = 10.73YY152 pKa = 8.84CDD154 pKa = 3.27WKK156 pKa = 10.89FAVIPAKK163 pKa = 10.52YY164 pKa = 8.54EE165 pKa = 3.92HH166 pKa = 7.29PPGITFFGGDD176 pKa = 3.58LDD178 pKa = 6.04GRR180 pKa = 11.84DD181 pKa = 3.79DD182 pKa = 3.7WLQQSGIGLGEE193 pKa = 4.2PLLGICLSICVYY205 pKa = 10.84GEE207 pKa = 3.84SRR209 pKa = 11.84TGKK212 pKa = 7.35TLWARR217 pKa = 11.84SLGSHH222 pKa = 7.11IYY224 pKa = 10.44CVGLVSGTEE233 pKa = 3.97CMKK236 pKa = 10.36ATEE239 pKa = 3.79ADD241 pKa = 3.68YY242 pKa = 11.48AVFDD246 pKa = 5.44DD247 pKa = 3.7IRR249 pKa = 11.84GGIKK253 pKa = 9.99FFPSFKK259 pKa = 10.33EE260 pKa = 3.72WLGCQAYY267 pKa = 7.85VTVKK271 pKa = 10.25CLYY274 pKa = 10.23RR275 pKa = 11.84EE276 pKa = 4.0PSLVKK281 pKa = 9.6WGKK284 pKa = 9.21PSIWLSNTDD293 pKa = 3.9PRR295 pKa = 11.84DD296 pKa = 3.34EE297 pKa = 4.42MLNADD302 pKa = 4.4IEE304 pKa = 4.54WMNKK308 pKa = 7.88NCIFVEE314 pKa = 4.28VNSAIFHH321 pKa = 6.72ANTT324 pKa = 3.54

Molecular weight: 36.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4U9K5|A0A0B4U9K5_9VIRU Capsid protein OS=Bromus-associated circular DNA virus 3 OX=1590156 PE=4 SV=1
MM1 pKa = 7.92RR2 pKa = 11.84SRR4 pKa = 11.84YY5 pKa = 6.94TKK7 pKa = 9.83RR8 pKa = 11.84SGFRR12 pKa = 11.84RR13 pKa = 11.84TKK15 pKa = 7.74RR16 pKa = 11.84TSRR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84SYY23 pKa = 10.79KK24 pKa = 9.86GRR26 pKa = 11.84TRR28 pKa = 11.84YY29 pKa = 7.36STRR32 pKa = 11.84RR33 pKa = 11.84VTKK36 pKa = 10.21RR37 pKa = 11.84LPQRR41 pKa = 11.84KK42 pKa = 7.61SRR44 pKa = 11.84KK45 pKa = 8.83MILNLTSKK53 pKa = 10.52KK54 pKa = 10.32KK55 pKa = 10.13RR56 pKa = 11.84DD57 pKa = 3.34TMAPNTGYY65 pKa = 9.33PAATNQIRR73 pKa = 11.84GLEE76 pKa = 4.03IQGGNVATITLWSPTARR93 pKa = 11.84EE94 pKa = 3.6LRR96 pKa = 11.84EE97 pKa = 3.59IFEE100 pKa = 4.49NDD102 pKa = 3.16KK103 pKa = 11.43SSVPSGRR110 pKa = 11.84EE111 pKa = 3.51ATNCYY116 pKa = 9.85IVGLKK121 pKa = 10.16EE122 pKa = 4.41KK123 pKa = 10.57ISILTNSGNPWKK135 pKa = 9.74WRR137 pKa = 11.84RR138 pKa = 11.84IAFTHH143 pKa = 6.85KK144 pKa = 10.49GLLPLGEE151 pKa = 4.14QFEE154 pKa = 4.56GSRR157 pKa = 11.84VYY159 pKa = 10.08STITDD164 pKa = 3.41VVGRR168 pKa = 11.84ITYY171 pKa = 9.95YY172 pKa = 9.51RR173 pKa = 11.84TLTPLPDD180 pKa = 3.47TSKK183 pKa = 9.96TPLVDD188 pKa = 3.81YY189 pKa = 10.28LFKK192 pKa = 11.12GQGEE196 pKa = 4.63GGLGVQDD203 pKa = 3.17WTDD206 pKa = 3.48IITAPVDD213 pKa = 3.31TTRR216 pKa = 11.84IKK218 pKa = 10.95VMHH221 pKa = 7.08DD222 pKa = 2.98HH223 pKa = 6.41THH225 pKa = 6.6PHH227 pKa = 6.55PKK229 pKa = 9.04WKK231 pKa = 10.45

Molecular weight: 26.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

555

231

324

277.5

31.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.045 ± 0.971

2.883 ± 1.504

5.946 ± 1.259

5.045 ± 0.706

3.964 ± 1.105

8.829 ± 0.637

2.703 ± 0.33

5.586 ± 0.292

6.667 ± 0.957

7.207 ± 0.172

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.622 ± 0.068

2.703 ± 0.467

5.045 ± 0.358

2.523 ± 0.046

8.108 ± 2.198

7.027 ± 0.062

7.387 ± 2.907

5.405 ± 0.395

2.523 ± 0.22

3.784 ± 0.069
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski