CRESS virus sp. ctmn412
Average proteome isoelectric point is 8.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5Q2W5N0|A0A5Q2W5N0_9VIRU Rep protein OS=CRESS virus sp. ctmn412 OX=2656688 PE=4 SV=1
MM1 pKa = 7.54 ARR3 pKa = 11.84 QSNSKK8 pKa = 9.66 KK9 pKa = 9.5 PRR11 pKa = 11.84 VNRR14 pKa = 11.84 SRR16 pKa = 11.84 SWFFTYY22 pKa = 10.82 NNPPEE27 pKa = 4.32 NAWAQIIDD35 pKa = 3.88 TFEE38 pKa = 4.19 NDD40 pKa = 2.63 QCEE43 pKa = 4.21 YY44 pKa = 11.09 VFQLEE49 pKa = 4.29 QGDD52 pKa = 4.16 TVHH55 pKa = 5.77 YY56 pKa = 10.08 QGVIRR61 pKa = 11.84 YY62 pKa = 7.48 KK63 pKa = 10.57 NPRR66 pKa = 11.84 EE67 pKa = 3.68 NWPQISCHH75 pKa = 5.35 WEE77 pKa = 3.71 RR78 pKa = 11.84 CISWRR83 pKa = 11.84 SAIKK87 pKa = 10.51 YY88 pKa = 8.62 CSKK91 pKa = 9.91 VQTRR95 pKa = 11.84 IDD97 pKa = 4.11 GPWTNIDD104 pKa = 3.36 GLKK107 pKa = 10.55 FRR109 pKa = 11.84 DD110 pKa = 4.06 TIKK113 pKa = 11.15 DD114 pKa = 3.28 PLQNKK119 pKa = 9.1 KK120 pKa = 10.47 LYY122 pKa = 9.83 PFQQEE127 pKa = 3.57 ILDD130 pKa = 4.27 IIKK133 pKa = 9.59 TDD135 pKa = 3.34 SNDD138 pKa = 3.82 RR139 pKa = 11.84 VIYY142 pKa = 8.88 WYY144 pKa = 9.54 WEE146 pKa = 4.01 PNGNTGKK153 pKa = 10.69 SSLCKK158 pKa = 10.22 HH159 pKa = 5.25 IMLRR163 pKa = 11.84 YY164 pKa = 8.15 KK165 pKa = 10.54 ACLIGGAAKK174 pKa = 10.77 DD175 pKa = 3.62 NFHH178 pKa = 6.74 CVSSYY183 pKa = 11.64 LEE185 pKa = 4.91 DD186 pKa = 3.98 NDD188 pKa = 5.73 LKK190 pKa = 11.39 VCLIDD195 pKa = 4.81 IPRR198 pKa = 11.84 CSMNSVSYY206 pKa = 8.56 KK207 pKa = 10.04 TIEE210 pKa = 4.47 CIKK213 pKa = 10.7 NGIIFSGKK221 pKa = 9.63 YY222 pKa = 7.86 EE223 pKa = 4.37 SNYY226 pKa = 10.62 KK227 pKa = 9.99 IFNSPHH233 pKa = 7.25 LIILANFPPNTDD245 pKa = 3.23 MLSEE249 pKa = 4.32 DD250 pKa = 2.89 RR251 pKa = 11.84 WVVKK255 pKa = 10.53 RR256 pKa = 11.84 II257 pKa = 3.46
Molecular weight: 30.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.704
IPC2_protein 7.907
IPC_protein 7.746
Toseland 7.322
ProMoST 8.302
Dawson 8.463
Bjellqvist 8.814
Wikipedia 8.39
Rodwell 8.492
Grimsley 7.293
Solomon 8.536
Lehninger 8.551
Nozaki 9.019
DTASelect 8.536
Thurlkill 8.595
EMBOSS 8.653
Sillero 8.916
Patrickios 4.113
IPC_peptide 8.536
IPC2_peptide 7.805
IPC2.peptide.svr19 7.996
Protein with the highest isoelectric point:
>tr|A0A5Q2W5N0|A0A5Q2W5N0_9VIRU Rep protein OS=CRESS virus sp. ctmn412 OX=2656688 PE=4 SV=1
MM1 pKa = 6.92 SAYY4 pKa = 10.2 RR5 pKa = 11.84 GRR7 pKa = 11.84 RR8 pKa = 11.84 KK9 pKa = 10.09 RR10 pKa = 11.84 KK11 pKa = 8.01 FGYY14 pKa = 9.38 RR15 pKa = 11.84 KK16 pKa = 9.55 KK17 pKa = 10.3 SYY19 pKa = 10.2 RR20 pKa = 11.84 RR21 pKa = 11.84 NAEE24 pKa = 3.87 DD25 pKa = 3.75 KK26 pKa = 10.69 RR27 pKa = 11.84 IARR30 pKa = 11.84 IAKK33 pKa = 9.87 AVTSKK38 pKa = 11.04 AKK40 pKa = 9.4 EE41 pKa = 3.75 LKK43 pKa = 10.66 YY44 pKa = 10.77 YY45 pKa = 9.69 DD46 pKa = 4.14 TIITNNTPVSGTSLVSILDD65 pKa = 3.79 DD66 pKa = 3.39 MAQGDD71 pKa = 4.34 TEE73 pKa = 4.27 STRR76 pKa = 11.84 DD77 pKa = 3.29 GNYY80 pKa = 10.02 IIIKK84 pKa = 9.2 SINVKK89 pKa = 10.46 GYY91 pKa = 10.89 VYY93 pKa = 10.52 DD94 pKa = 3.61 QGAGPRR100 pKa = 11.84 DD101 pKa = 3.89 TICRR105 pKa = 11.84 MILIRR110 pKa = 11.84 EE111 pKa = 4.16 KK112 pKa = 10.86 VDD114 pKa = 3.6 CEE116 pKa = 4.27 GNLPLVGDD124 pKa = 4.83 LLDD127 pKa = 3.87 TDD129 pKa = 5.87 AIPSLRR135 pKa = 11.84 YY136 pKa = 7.77 WPNMGNFQVWWDD148 pKa = 3.35 KK149 pKa = 10.94 TFVIRR154 pKa = 11.84 ASDD157 pKa = 4.19 DD158 pKa = 3.64 YY159 pKa = 11.51 VTDD162 pKa = 4.03 QIQHH166 pKa = 5.11 SRR168 pKa = 11.84 LVKK171 pKa = 10.07 YY172 pKa = 9.69 YY173 pKa = 10.51 KK174 pKa = 9.95 KK175 pKa = 10.41 AKK177 pKa = 9.81 KK178 pKa = 9.52 PIRR181 pKa = 11.84 AYY183 pKa = 10.81 YY184 pKa = 10.27 DD185 pKa = 3.32 GVGPGVGTCKK195 pKa = 10.0 RR196 pKa = 11.84 GHH198 pKa = 6.36 FFLITMTDD206 pKa = 3.12 QVAGNRR212 pKa = 11.84 PNWDD216 pKa = 3.28 LKK218 pKa = 10.8 VRR220 pKa = 11.84 ITFTEE225 pKa = 3.88
Molecular weight: 25.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.9
IPC2_protein 9.194
IPC_protein 9.165
Toseland 9.721
ProMoST 9.502
Dawson 9.999
Bjellqvist 9.706
Wikipedia 10.189
Rodwell 10.292
Grimsley 10.087
Solomon 10.014
Lehninger 9.97
Nozaki 9.75
DTASelect 9.692
Thurlkill 9.823
EMBOSS 10.145
Sillero 9.911
Patrickios 5.283
IPC_peptide 10.014
IPC2_peptide 8.361
IPC2.peptide.svr19 8.161
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
482
225
257
241.0
28.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.357 ± 0.947
2.697 ± 0.909
7.261 ± 0.788
3.734 ± 0.711
3.32 ± 0.435
5.602 ± 1.302
1.452 ± 0.375
8.921 ± 0.614
8.299 ± 0.393
5.394 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.867 ± 0.237
6.432 ± 1.324
4.357 ± 0.534
3.527 ± 0.573
7.261 ± 1.084
6.432 ± 1.028
5.394 ± 1.144
5.394 ± 0.848
2.697 ± 0.612
5.602 ± 0.413
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here