Amino acid dipepetide frequency for Thiothrix nivea (strain ATCC 35100 / DSM 5205 / JP2)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.912AlaAla: 10.912 ± 0.149	1.185AlaCys: 1.185 ± 0.035	5.91AlaAsp: 5.91 ± 0.077	6.548AlaGlu: 6.548 ± 0.082	3.498AlaPhe: 3.498 ± 0.058	8.028AlaGly: 8.028 ± 0.113	1.971AlaHis: 1.971 ± 0.037	5.222AlaIle: 5.222 ± 0.062	3.88AlaLys: 3.88 ± 0.061	10.352AlaLeu: 10.352 ± 0.114	2.849AlaMet: 2.849 ± 0.051	3.658AlaAsn: 3.658 ± 0.064	3.703AlaPro: 3.703 ± 0.067	4.172AlaGln: 4.172 ± 0.058	5.13AlaArg: 5.13 ± 0.069	5.765AlaSer: 5.765 ± 0.084	5.117AlaThr: 5.117 ± 0.095	6.646AlaVal: 6.646 ± 0.074	1.413AlaTrp: 1.413 ± 0.037	2.662AlaTyr: 2.662 ± 0.053	0.0AlaXaa: 0.0 ± 0.0
Cys	0.801CysAla: 0.801 ± 0.025	0.144CysCys: 0.144 ± 0.012	0.502CysAsp: 0.502 ± 0.02	0.547CysGlu: 0.547 ± 0.02	0.362CysPhe: 0.362 ± 0.016	0.973CysGly: 0.973 ± 0.031	0.351CysHis: 0.351 ± 0.018	0.493CysIle: 0.493 ± 0.019	0.422CysLys: 0.422 ± 0.019	1.003CysLeu: 1.003 ± 0.027	0.22CysMet: 0.22 ± 0.012	0.333CysAsn: 0.333 ± 0.015	0.578CysPro: 0.578 ± 0.026	0.455CysGln: 0.455 ± 0.018	0.591CysArg: 0.591 ± 0.019	0.608CysSer: 0.608 ± 0.025	0.515CysThr: 0.515 ± 0.018	0.633CysVal: 0.633 ± 0.023	0.144CysTrp: 0.144 ± 0.011	0.29CysTyr: 0.29 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	5.801AspAla: 5.801 ± 0.081	0.518AspCys: 0.518 ± 0.02	2.965AspAsp: 2.965 ± 0.062	3.496AspGlu: 3.496 ± 0.065	2.333AspPhe: 2.333 ± 0.039	4.646AspGly: 4.646 ± 0.14	1.026AspHis: 1.026 ± 0.029	3.362AspIle: 3.362 ± 0.052	2.849AspLys: 2.849 ± 0.058	5.083AspLeu: 5.083 ± 0.061	1.602AspMet: 1.602 ± 0.035	2.213AspAsn: 2.213 ± 0.046	2.528AspPro: 2.528 ± 0.048	1.776AspGln: 1.776 ± 0.035	2.507AspArg: 2.507 ± 0.048	3.059AspSer: 3.059 ± 0.055	3.138AspThr: 3.138 ± 0.064	3.712AspVal: 3.712 ± 0.049	0.971AspTrp: 0.971 ± 0.031	1.942AspTyr: 1.942 ± 0.039	0.0AspXaa: 0.0 ± 0.0
Glu	6.107GluAla: 6.107 ± 0.081	0.512GluCys: 0.512 ± 0.02	2.856GluAsp: 2.856 ± 0.05	3.506GluGlu: 3.506 ± 0.065	2.073GluPhe: 2.073 ± 0.038	3.831GluGly: 3.831 ± 0.056	1.509GluHis: 1.509 ± 0.038	3.284GluIle: 3.284 ± 0.052	3.071GluLys: 3.071 ± 0.055	6.279GluLeu: 6.279 ± 0.091	1.613GluMet: 1.613 ± 0.036	2.265GluAsn: 2.265 ± 0.047	2.419GluPro: 2.419 ± 0.051	3.537GluGln: 3.537 ± 0.063	3.959GluArg: 3.959 ± 0.068	2.842GluSer: 2.842 ± 0.049	3.517GluThr: 3.517 ± 0.052	3.91GluVal: 3.91 ± 0.06	0.869GluTrp: 0.869 ± 0.027	1.807GluTyr: 1.807 ± 0.039	0.0GluXaa: 0.0 ± 0.0
Phe	3.724PheAla: 3.724 ± 0.058	0.433PheCys: 0.433 ± 0.018	2.278PheAsp: 2.278 ± 0.037	1.991PheGlu: 1.991 ± 0.04	1.521PhePhe: 1.521 ± 0.041	3.04PheGly: 3.04 ± 0.055	0.915PheHis: 0.915 ± 0.028	2.103PheIle: 2.103 ± 0.04	1.404PheLys: 1.404 ± 0.034	3.669PheLeu: 3.669 ± 0.068	1.032PheMet: 1.032 ± 0.026	1.535PheAsn: 1.535 ± 0.042	1.646PhePro: 1.646 ± 0.042	1.487PheGln: 1.487 ± 0.036	2.082PheArg: 2.082 ± 0.05	2.694PheSer: 2.694 ± 0.054	2.14PheThr: 2.14 ± 0.042	2.338PheVal: 2.338 ± 0.048	0.572PheTrp: 0.572 ± 0.021	1.203PheTyr: 1.203 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	5.832GlyAla: 5.832 ± 0.114	0.956GlyCys: 0.956 ± 0.032	3.987GlyAsp: 3.987 ± 0.081	4.624GlyGlu: 4.624 ± 0.06	3.19GlyPhe: 3.19 ± 0.06	5.91GlyGly: 5.91 ± 0.088	1.743GlyHis: 1.743 ± 0.037	4.738GlyIle: 4.738 ± 0.067	4.864GlyLys: 4.864 ± 0.094	7.607GlyLeu: 7.607 ± 0.094	2.43GlyMet: 2.43 ± 0.047	3.231GlyAsn: 3.231 ± 0.088	1.52GlyPro: 1.52 ± 0.038	3.27GlyGln: 3.27 ± 0.051	3.773GlyArg: 3.773 ± 0.066	4.74GlySer: 4.74 ± 0.101	3.748GlyThr: 3.748 ± 0.081	5.691GlyVal: 5.691 ± 0.078	1.293GlyTrp: 1.293 ± 0.033	2.609GlyTyr: 2.609 ± 0.051	0.0GlyXaa: 0.0 ± 0.0
His	2.114HisAla: 2.114 ± 0.042	0.319HisCys: 0.319 ± 0.015	1.355HisAsp: 1.355 ± 0.036	1.302HisGlu: 1.302 ± 0.028	1.07HisPhe: 1.07 ± 0.029	1.806HisGly: 1.806 ± 0.039	0.733HisHis: 0.733 ± 0.024	1.369HisIle: 1.369 ± 0.035	0.99HisLys: 0.99 ± 0.029	2.546HisLeu: 2.546 ± 0.05	0.569HisMet: 0.569 ± 0.023	0.822HisAsn: 0.822 ± 0.025	1.45HisPro: 1.45 ± 0.031	1.003HisGln: 1.003 ± 0.029	1.183HisArg: 1.183 ± 0.03	1.262HisSer: 1.262 ± 0.032	1.245HisThr: 1.245 ± 0.03	1.4HisVal: 1.4 ± 0.032	0.458HisTrp: 0.458 ± 0.017	0.926HisTyr: 0.926 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	5.39IleAla: 5.39 ± 0.069	0.518IleCys: 0.518 ± 0.02	3.165IleAsp: 3.165 ± 0.062	3.098IleGlu: 3.098 ± 0.052	1.806IlePhe: 1.806 ± 0.042	4.015IleGly: 4.015 ± 0.061	1.32IleHis: 1.32 ± 0.03	2.843IleIle: 2.843 ± 0.052	2.152IleLys: 2.152 ± 0.046	5.208IleLeu: 5.208 ± 0.078	1.17IleMet: 1.17 ± 0.032	2.204IleAsn: 2.204 ± 0.041	2.994IlePro: 2.994 ± 0.048	2.079IleGln: 2.079 ± 0.044	3.514IleArg: 3.514 ± 0.055	3.401IleSer: 3.401 ± 0.052	3.178IleThr: 3.178 ± 0.058	3.27IleVal: 3.27 ± 0.05	0.542IleTrp: 0.542 ± 0.019	1.344IleTyr: 1.344 ± 0.035	0.0IleXaa: 0.0 ± 0.0
Lys	4.473LysAla: 4.473 ± 0.073	0.274LysCys: 0.274 ± 0.014	2.292LysAsp: 2.292 ± 0.079	2.363LysGlu: 2.363 ± 0.051	1.184LysPhe: 1.184 ± 0.03	3.082LysGly: 3.082 ± 0.057	1.087LysHis: 1.087 ± 0.024	2.248LysIle: 2.248 ± 0.049	2.103LysLys: 2.103 ± 0.05	4.702LysLeu: 4.702 ± 0.063	1.097LysMet: 1.097 ± 0.034	1.532LysAsn: 1.532 ± 0.035	2.642LysPro: 2.642 ± 0.051	2.366LysGln: 2.366 ± 0.038	2.597LysArg: 2.597 ± 0.052	2.205LysSer: 2.205 ± 0.041	2.645LysThr: 2.645 ± 0.044	3.105LysVal: 3.105 ± 0.051	0.462LysTrp: 0.462 ± 0.018	1.128LysTyr: 1.128 ± 0.034	0.0LysXaa: 0.0 ± 0.0
Leu	11.182LeuAla: 11.182 ± 0.113	0.941LeuCys: 0.941 ± 0.027	6.026LeuAsp: 6.026 ± 0.077	6.753LeuGlu: 6.753 ± 0.087	4.146LeuPhe: 4.146 ± 0.074	7.622LeuGly: 7.622 ± 0.081	2.665LeuHis: 2.665 ± 0.046	4.945LeuIle: 4.945 ± 0.078	4.559LeuLys: 4.559 ± 0.072	12.774LeuLeu: 12.774 ± 0.172	2.589LeuMet: 2.589 ± 0.05	3.692LeuAsn: 3.692 ± 0.056	5.993LeuPro: 5.993 ± 0.076	5.074LeuGln: 5.074 ± 0.075	5.995LeuArg: 5.995 ± 0.091	6.166LeuSer: 6.166 ± 0.079	5.542LeuThr: 5.542 ± 0.072	6.742LeuVal: 6.742 ± 0.082	1.27LeuTrp: 1.27 ± 0.033	2.812LeuTyr: 2.812 ± 0.047	0.0LeuXaa: 0.0 ± 0.0
Met	2.613MetAla: 2.613 ± 0.047	0.165MetCys: 0.165 ± 0.012	1.384MetAsp: 1.384 ± 0.033	1.471MetGlu: 1.471 ± 0.033	0.793MetPhe: 0.793 ± 0.025	1.966MetGly: 1.966 ± 0.044	0.548MetHis: 0.548 ± 0.022	1.199MetIle: 1.199 ± 0.032	1.323MetLys: 1.323 ± 0.033	2.862MetLeu: 2.862 ± 0.049	0.693MetMet: 0.693 ± 0.027	1.038MetAsn: 1.038 ± 0.029	1.476MetPro: 1.476 ± 0.035	1.498MetGln: 1.498 ± 0.038	1.467MetArg: 1.467 ± 0.035	1.521MetSer: 1.521 ± 0.032	1.506MetThr: 1.506 ± 0.032	1.661MetVal: 1.661 ± 0.035	0.216MetTrp: 0.216 ± 0.014	0.484MetTyr: 0.484 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	3.506AsnAla: 3.506 ± 0.06	0.362AsnCys: 0.362 ± 0.019	1.868AsnAsp: 1.868 ± 0.048	1.671AsnGlu: 1.671 ± 0.034	1.27AsnPhe: 1.27 ± 0.032	3.098AsnGly: 3.098 ± 0.075	0.902AsnHis: 0.902 ± 0.025	2.25AsnIle: 2.25 ± 0.043	1.464AsnLys: 1.464 ± 0.042	3.955AsnLeu: 3.955 ± 0.065	0.931AsnMet: 0.931 ± 0.028	1.606AsnAsn: 1.606 ± 0.053	2.557AsnPro: 2.557 ± 0.046	1.56AsnGln: 1.56 ± 0.037	2.108AsnArg: 2.108 ± 0.044	1.984AsnSer: 1.984 ± 0.051	2.165AsnThr: 2.165 ± 0.053	2.21AsnVal: 2.21 ± 0.04	0.527AsnTrp: 0.527 ± 0.019	1.145AsnTyr: 1.145 ± 0.031	0.0AsnXaa: 0.0 ± 0.0
Pro	5.382ProAla: 5.382 ± 0.087	0.361ProCys: 0.361 ± 0.016	3.436ProAsp: 3.436 ± 0.06	3.739ProGlu: 3.739 ± 0.062	1.765ProPhe: 1.765 ± 0.041	3.36ProGly: 3.36 ± 0.061	1.049ProHis: 1.049 ± 0.03	2.007ProIle: 2.007 ± 0.043	1.615ProLys: 1.615 ± 0.036	4.815ProLeu: 4.815 ± 0.066	1.099ProMet: 1.099 ± 0.029	1.574ProAsn: 1.574 ± 0.037	2.414ProPro: 2.414 ± 0.054	2.062ProGln: 2.062 ± 0.042	2.026ProArg: 2.026 ± 0.046	2.326ProSer: 2.326 ± 0.047	2.434ProThr: 2.434 ± 0.05	3.968ProVal: 3.968 ± 0.062	0.657ProTrp: 0.657 ± 0.024	1.391ProTyr: 1.391 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	5.423GlnAla: 5.423 ± 0.074	0.384GlnCys: 0.384 ± 0.019	2.13GlnAsp: 2.13 ± 0.043	2.696GlnGlu: 2.696 ± 0.049	1.503GlnPhe: 1.503 ± 0.034	3.14GlnGly: 3.14 ± 0.052	1.406GlnHis: 1.406 ± 0.033	2.143GlnIle: 2.143 ± 0.039	1.81GlnLys: 1.81 ± 0.039	4.631GlnLeu: 4.631 ± 0.073	1.113GlnMet: 1.113 ± 0.028	1.374GlnAsn: 1.374 ± 0.029	2.403GlnPro: 2.403 ± 0.052	3.514GlnGln: 3.514 ± 0.08	3.089GlnArg: 3.089 ± 0.054	2.074GlnSer: 2.074 ± 0.041	2.504GlnThr: 2.504 ± 0.042	3.181GlnVal: 3.181 ± 0.049	0.693GlnTrp: 0.693 ± 0.026	1.328GlnTyr: 1.328 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	4.37ArgAla: 4.37 ± 0.071	0.539ArgCys: 0.539 ± 0.021	3.201ArgAsp: 3.201 ± 0.063	3.903ArgGlu: 3.903 ± 0.067	2.616ArgPhe: 2.616 ± 0.047	3.207ArgGly: 3.207 ± 0.049	1.575ArgHis: 1.575 ± 0.043	3.335ArgIle: 3.335 ± 0.055	2.736ArgLys: 2.736 ± 0.055	6.349ArgLeu: 6.349 ± 0.088	1.559ArgMet: 1.559 ± 0.039	2.247ArgAsn: 2.247 ± 0.048	2.208ArgPro: 2.208 ± 0.039	3.18ArgGln: 3.18 ± 0.058	3.153ArgArg: 3.153 ± 0.057	2.725ArgSer: 2.725 ± 0.044	2.442ArgThr: 2.442 ± 0.045	3.565ArgVal: 3.565 ± 0.057	0.872ArgTrp: 0.872 ± 0.026	1.977ArgTyr: 1.977 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	5.211SerAla: 5.211 ± 0.073	0.609SerCys: 0.609 ± 0.023	2.734SerAsp: 2.734 ± 0.046	2.692SerGlu: 2.692 ± 0.05	2.237SerPhe: 2.237 ± 0.042	5.368SerGly: 5.368 ± 0.081	1.389SerHis: 1.389 ± 0.035	3.223SerIle: 3.223 ± 0.061	2.207SerLys: 2.207 ± 0.047	6.416SerLeu: 6.416 ± 0.076	1.38SerMet: 1.38 ± 0.03	2.064SerAsn: 2.064 ± 0.057	2.739SerPro: 2.739 ± 0.054	2.236SerGln: 2.236 ± 0.045	3.165SerArg: 3.165 ± 0.055	3.632SerSer: 3.632 ± 0.068	3.19SerThr: 3.19 ± 0.063	3.772SerVal: 3.772 ± 0.054	0.77SerTrp: 0.77 ± 0.024	1.526SerTyr: 1.526 ± 0.036	0.0SerXaa: 0.0 ± 0.0
Thr	5.461ThrAla: 5.461 ± 0.09	0.517ThrCys: 0.517 ± 0.019	3.233ThrAsp: 3.233 ± 0.068	2.792ThrGlu: 2.792 ± 0.044	1.902ThrPhe: 1.902 ± 0.039	4.829ThrGly: 4.829 ± 0.11	1.335ThrHis: 1.335 ± 0.032	2.705ThrIle: 2.705 ± 0.048	1.501ThrLys: 1.501 ± 0.039	6.717ThrLeu: 6.717 ± 0.087	1.015ThrMet: 1.015 ± 0.031	1.722ThrAsn: 1.722 ± 0.051	3.32ThrPro: 3.32 ± 0.054	2.228ThrGln: 2.228 ± 0.042	2.845ThrArg: 2.845 ± 0.05	2.988ThrSer: 2.988 ± 0.06	3.096ThrThr: 3.096 ± 0.076	3.951ThrVal: 3.951 ± 0.081	0.633ThrTrp: 0.633 ± 0.023	1.413ThrTyr: 1.413 ± 0.035	0.0ThrXaa: 0.0 ± 0.0
Val	6.531ValAla: 6.531 ± 0.087	0.751ValCys: 0.751 ± 0.028	3.962ValAsp: 3.962 ± 0.06	4.289ValGlu: 4.289 ± 0.064	2.721ValPhe: 2.721 ± 0.049	4.722ValGly: 4.722 ± 0.075	1.311ValHis: 1.311 ± 0.034	3.797ValIle: 3.797 ± 0.053	2.915ValLys: 2.915 ± 0.051	7.189ValLeu: 7.189 ± 0.085	1.934ValMet: 1.934 ± 0.042	2.416ValAsn: 2.416 ± 0.053	2.84ValPro: 2.84 ± 0.051	2.571ValGln: 2.571 ± 0.045	3.667ValArg: 3.667 ± 0.058	4.242ValSer: 4.242 ± 0.067	3.945ValThr: 3.945 ± 0.077	4.853ValVal: 4.853 ± 0.074	0.93ValTrp: 0.93 ± 0.026	1.869ValTyr: 1.869 ± 0.039	0.0ValXaa: 0.0 ± 0.0
Trp	1.02TrpAla: 1.02 ± 0.03	0.135TrpCys: 0.135 ± 0.009	0.685TrpAsp: 0.685 ± 0.024	0.865TrpGlu: 0.865 ± 0.027	0.579TrpPhe: 0.579 ± 0.022	0.915TrpGly: 0.915 ± 0.03	0.368TrpHis: 0.368 ± 0.017	0.597TrpIle: 0.597 ± 0.025	0.654TrpLys: 0.654 ± 0.02	2.084TrpLeu: 2.084 ± 0.05	0.389TrpMet: 0.389 ± 0.015	0.518TrpAsn: 0.518 ± 0.023	0.519TrpPro: 0.519 ± 0.021	0.932TrpGln: 0.932 ± 0.026	0.895TrpArg: 0.895 ± 0.027	0.664TrpSer: 0.664 ± 0.024	0.525TrpThr: 0.525 ± 0.022	1.038TrpVal: 1.038 ± 0.029	0.263TrpTrp: 0.263 ± 0.016	0.408TrpTyr: 0.408 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.767TyrAla: 2.767 ± 0.048	0.343TyrCys: 0.343 ± 0.017	1.56TyrAsp: 1.56 ± 0.046	1.422TyrGlu: 1.422 ± 0.032	1.237TyrPhe: 1.237 ± 0.027	2.199TyrGly: 2.199 ± 0.046	0.716TyrHis: 0.716 ± 0.023	1.349TyrIle: 1.349 ± 0.034	1.067TyrLys: 1.067 ± 0.028	3.249TyrLeu: 3.249 ± 0.051	0.601TyrMet: 0.601 ± 0.023	1.096TyrAsn: 1.096 ± 0.031	1.531TyrPro: 1.531 ± 0.035	1.539TyrGln: 1.539 ± 0.034	2.013TyrArg: 2.013 ± 0.04	1.66TyrSer: 1.66 ± 0.034	1.611TyrThr: 1.611 ± 0.037	1.806TyrVal: 1.806 ± 0.034	0.499TyrTrp: 0.499 ± 0.022	1.02TyrTyr: 1.02 ± 0.027	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4218 proteins (1343628 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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