Phaeomoniella chlamydospora
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6907 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0G2E4V8|A0A0G2E4V8_9EURO Putative fes cip4 homology domain protein OS=Phaeomoniella chlamydospora OX=158046 GN=UCRPC4_g05387 PE=4 SV=1
MM1 pKa = 7.77 PSYY4 pKa = 10.58 KK5 pKa = 10.25 FSLAVIALSSASAVLSNPIAAPAASPAPTSAPDD38 pKa = 3.18 INKK41 pKa = 9.83 RR42 pKa = 11.84 ATTCTFTGSKK52 pKa = 9.84 GASFASKK59 pKa = 10.32 SKK61 pKa = 10.38 SSCSTIVLSAVAVPSGTTLDD81 pKa = 3.84 LTDD84 pKa = 5.38 LEE86 pKa = 5.69 DD87 pKa = 3.97 GTHH90 pKa = 6.7 TDD92 pKa = 5.8 LIMTDD97 pKa = 3.07 ITIDD101 pKa = 4.02 NSDD104 pKa = 3.6 GDD106 pKa = 4.54 DD107 pKa = 3.42 NGAANTDD114 pKa = 3.35 GFDD117 pKa = 3.51 IGDD120 pKa = 3.73 STGFTIKK127 pKa = 10.53 SATVYY132 pKa = 10.12 NQHH135 pKa = 6.91 DD136 pKa = 4.0 CVAINSGEE144 pKa = 4.18 TIVFSGGYY152 pKa = 9.47 CSGGHH157 pKa = 5.68 GLSVGSVGGRR167 pKa = 11.84 SDD169 pKa = 3.5 NTVKK173 pKa = 10.7 GVTFEE178 pKa = 4.06 SSTVINSQNGVRR190 pKa = 11.84 IKK192 pKa = 9.73 TVYY195 pKa = 10.39 DD196 pKa = 3.24 ATGEE200 pKa = 4.39 VTDD203 pKa = 3.97 VTYY206 pKa = 11.33 KK207 pKa = 10.93 DD208 pKa = 3.26 ITLSGITKK216 pKa = 8.48 YY217 pKa = 10.97 GIVIRR222 pKa = 11.84 QDD224 pKa = 3.55 YY225 pKa = 10.69 EE226 pKa = 4.08 NGSPTGDD233 pKa = 3.57 PTTGVPITDD242 pKa = 3.71 CTFDD246 pKa = 4.26 EE247 pKa = 4.27 ITGTVEE253 pKa = 3.51 SDD255 pKa = 2.95 AVEE258 pKa = 4.08 IFILCGDD265 pKa = 4.83 DD266 pKa = 4.87 SCSDD270 pKa = 3.61 WTWEE274 pKa = 5.14 DD275 pKa = 2.81 IDD277 pKa = 3.69 ITGGEE282 pKa = 4.21 TSDD285 pKa = 3.46 GCEE288 pKa = 3.96 NVPSGASCSEE298 pKa = 3.98
Molecular weight: 30.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.696
IPC2_protein 3.846
IPC_protein 3.859
Toseland 3.63
ProMoST 4.012
Dawson 3.872
Bjellqvist 4.05
Wikipedia 3.821
Rodwell 3.681
Grimsley 3.541
Solomon 3.859
Lehninger 3.821
Nozaki 3.973
DTASelect 4.253
Thurlkill 3.694
EMBOSS 3.821
Sillero 3.986
Patrickios 1.24
IPC_peptide 3.859
IPC2_peptide 3.961
IPC2.peptide.svr19 3.892
Protein with the highest isoelectric point:
>tr|A0A0G2F1E5|A0A0G2F1E5_9EURO Putative exportin OS=Phaeomoniella chlamydospora OX=158046 GN=UCRPC4_g00438 PE=3 SV=1
MM1 pKa = 7.69 ASTRR5 pKa = 11.84 SLYY8 pKa = 11.08 KK9 pKa = 9.68 NTADD13 pKa = 4.08 DD14 pKa = 3.69 RR15 pKa = 11.84 SGIIIVITYY24 pKa = 10.5 VMLSVSTFLLLLQAGVGIYY43 pKa = 10.22 LKK45 pKa = 10.53 HH46 pKa = 6.21 PKK48 pKa = 9.77 GVNDD52 pKa = 4.04 ILLHH56 pKa = 5.45 LAGVFSIAQSIAITLSATNGLGRR79 pKa = 11.84 HH80 pKa = 5.14 RR81 pKa = 11.84 HH82 pKa = 5.87 DD83 pKa = 4.65 LSNGQFNRR91 pKa = 11.84 FSKK94 pKa = 10.54 FYY96 pKa = 10.29 YY97 pKa = 10.13 AGQICQIVAVGLSKK111 pKa = 10.97 LSVIIIINRR120 pKa = 11.84 LSSNRR125 pKa = 11.84 KK126 pKa = 8.72 VILACRR132 pKa = 11.84 CFAVVIIIYY141 pKa = 10.19 ILFSTLTTAFQCRR154 pKa = 11.84 FPFWTFAPNRR164 pKa = 11.84 CVLRR168 pKa = 11.84 GNLLYY173 pKa = 10.76 AISDD177 pKa = 3.95 SNALLDD183 pKa = 3.51 IVLIILPIVAIIQYY197 pKa = 10.13 KK198 pKa = 9.59 PRR200 pKa = 11.84 LPQRR204 pKa = 11.84 GFLIASFITRR214 pKa = 11.84 AAPLAAAILSLVSLPTYY231 pKa = 10.28 LHH233 pKa = 5.47 NTKK236 pKa = 9.3 DD237 pKa = 3.92 TTWTILPISTINEE250 pKa = 3.92 ILVHH254 pKa = 6.01 LNIIVACIPSILLISLNPPLPSNRR278 pKa = 11.84 EE279 pKa = 3.84 TASNNPIHH287 pKa = 6.98 RR288 pKa = 11.84 HH289 pKa = 5.32 RR290 pKa = 11.84 PPPFQKK296 pKa = 10.62 LPSHH300 pKa = 6.7 TNTTIRR306 pKa = 11.84 PSTPLFRR313 pKa = 11.84 YY314 pKa = 8.53 AHH316 pKa = 6.26 NFSPIQSFFHH326 pKa = 6.41 ISGGRR331 pKa = 11.84 EE332 pKa = 3.64 ACDD335 pKa = 3.2 NNNNRR340 pKa = 11.84 RR341 pKa = 11.84 SDD343 pKa = 3.34 RR344 pKa = 11.84 RR345 pKa = 11.84 GPNNNNNTSKK355 pKa = 9.17 YY356 pKa = 7.84 TGGGGVAGRR365 pKa = 11.84 RR366 pKa = 11.84 RR367 pKa = 11.84 RR368 pKa = 11.84 GDD370 pKa = 3.1 EE371 pKa = 4.03 DD372 pKa = 3.79 NNNNTTRR379 pKa = 11.84 SPTKK383 pKa = 9.31 RR384 pKa = 11.84 KK385 pKa = 10.51 SMADD389 pKa = 3.25 SLRR392 pKa = 11.84 TLTSFKK398 pKa = 10.59 GVRR401 pKa = 11.84 KK402 pKa = 7.56 TMEE405 pKa = 3.54 VRR407 pKa = 11.84 VEE409 pKa = 3.63 IEE411 pKa = 3.6 EE412 pKa = 4.31 RR413 pKa = 11.84 GVSRR417 pKa = 11.84 RR418 pKa = 11.84 GGGIGGG424 pKa = 3.68
Molecular weight: 46.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.339
IPC2_protein 9.853
IPC_protein 10.804
Toseland 10.789
ProMoST 10.613
Dawson 10.891
Bjellqvist 10.672
Wikipedia 11.155
Rodwell 11.008
Grimsley 10.95
Solomon 11.052
Lehninger 11.008
Nozaki 10.789
DTASelect 10.657
Thurlkill 10.804
EMBOSS 11.213
Sillero 10.833
Patrickios 10.687
IPC_peptide 11.052
IPC2_peptide 9.882
IPC2.peptide.svr19 8.407
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6907
0
6907
3771177
66
5663
546.0
60.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.238 ± 0.021
1.019 ± 0.008
5.933 ± 0.018
6.469 ± 0.025
3.711 ± 0.017
6.816 ± 0.027
2.272 ± 0.012
5.284 ± 0.019
5.281 ± 0.022
8.801 ± 0.031
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.082 ± 0.01
3.888 ± 0.015
5.699 ± 0.027
4.031 ± 0.018
5.742 ± 0.025
8.427 ± 0.03
6.182 ± 0.022
5.973 ± 0.018
1.346 ± 0.009
2.804 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here