Trametes versicolor (strain FP-101664) (White-rot fungus) (Coriolus versicolor)
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1022 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R7S7K5|R7S7K5_TRAVS Phosphoglucomutase OS=Trametes versicolor (strain FP-101664) OX=717944 GN=TRAVEDRAFT_157183 PE=3 SV=1
LL1 pKa = 7.11 LATIISLFFASAPVGAFAVLQIDD24 pKa = 4.33 TPSPAPTQCAPAILQWSGGAPPYY47 pKa = 10.34 FLAIVGTGDD56 pKa = 4.19 LAVALQRR63 pKa = 11.84 YY64 pKa = 9.23 DD65 pKa = 3.75 GLFDD69 pKa = 4.42 TSFLWSTNITARR81 pKa = 11.84 TSIALTVTDD90 pKa = 3.5 VTGTLAQSAPFIIHH104 pKa = 6.14 SSSDD108 pKa = 3.25 ISCLEE113 pKa = 3.78 SSVPSSSDD121 pKa = 2.41 ISRR124 pKa = 11.84 VLDD127 pKa = 3.35 STTFEE132 pKa = 4.81 ASTSMFPAGITAGASAGVSGGAGATAVATAGVV164 pKa = 3.56
Molecular weight: 16.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.74
IPC2_protein 3.884
IPC_protein 3.808
Toseland 3.592
ProMoST 4.024
Dawson 3.834
Bjellqvist 3.999
Wikipedia 3.834
Rodwell 3.643
Grimsley 3.503
Solomon 3.808
Lehninger 3.757
Nozaki 3.973
DTASelect 4.24
Thurlkill 3.681
EMBOSS 3.834
Sillero 3.935
Patrickios 1.926
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.853
Protein with the highest isoelectric point:
>tr|R7S788|R7S788_TRAVS Nitric oxide dioxygenase OS=Trametes versicolor (strain FP-101664) OX=717944 GN=TRAVEDRAFT_136661 PE=3 SV=1
MM1 pKa = 7.8 PSLRR5 pKa = 11.84 LPFSCMALSLLDD17 pKa = 3.47 GHH19 pKa = 6.85 TDD21 pKa = 3.31 TSSPAALAAHH31 pKa = 6.05 TRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 PSPASSTLPEE45 pKa = 4.05 SLAPYY50 pKa = 7.53 STPLDD55 pKa = 4.16 DD56 pKa = 3.89 PTTPRR61 pKa = 11.84 TSLRR65 pKa = 11.84 LWDD68 pKa = 3.73 LHH70 pKa = 6.37 SALSFALLDD79 pKa = 3.89 VLDD82 pKa = 4.52 VPAAPSLGQQAGLASRR98 pKa = 11.84 KK99 pKa = 5.27 TTPFGAANLARR110 pKa = 11.84 IMHH113 pKa = 7.15 RR114 pKa = 11.84 STPRR118 pKa = 11.84 PQRR121 pKa = 11.84 AASGKK126 pKa = 8.05 NWQVLASYY134 pKa = 7.95 STSWDD139 pKa = 3.07 RR140 pKa = 11.84 LRR142 pKa = 11.84 ALTSTGSRR150 pKa = 11.84 KK151 pKa = 9.33 NASDD155 pKa = 3.95 PRR157 pKa = 11.84 SCSQTRR163 pKa = 11.84 IWAHH167 pKa = 6.0 HH168 pKa = 5.43 GPNRR172 pKa = 11.84 RR173 pKa = 11.84 RR174 pKa = 11.84 AICAANTDD182 pKa = 3.56 SCDD185 pKa = 3.45 ARR187 pKa = 11.84 QHH189 pKa = 5.33 SQHH192 pKa = 6.24 TSSGIQPAYY201 pKa = 10.48 RR202 pKa = 11.84 PGSPPAPKK210 pKa = 9.03 TANTHH215 pKa = 4.34 HH216 pKa = 7.43 TYY218 pKa = 8.71 THH220 pKa = 6.17 TCQTILHH227 pKa = 7.06 ALQDD231 pKa = 3.51 VALAPKK237 pKa = 10.11 AVADD241 pKa = 3.98 RR242 pKa = 11.84 CFTILII248 pKa = 4.4
Molecular weight: 26.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.443
IPC_protein 10.262
Toseland 10.335
ProMoST 10.116
Dawson 10.496
Bjellqvist 10.277
Wikipedia 10.73
Rodwell 10.584
Grimsley 10.57
Solomon 10.599
Lehninger 10.57
Nozaki 10.423
DTASelect 10.248
Thurlkill 10.379
EMBOSS 10.76
Sillero 10.452
Patrickios 10.277
IPC_peptide 10.599
IPC2_peptide 9.677
IPC2.peptide.svr19 8.44
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1022
0
1022
359438
49
3380
351.7
38.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.894 ± 0.087
1.34 ± 0.034
5.96 ± 0.064
5.916 ± 0.08
3.52 ± 0.054
6.47 ± 0.075
2.742 ± 0.04
4.323 ± 0.054
3.904 ± 0.065
9.022 ± 0.079
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.064 ± 0.033
2.923 ± 0.038
6.912 ± 0.1
3.593 ± 0.053
6.934 ± 0.069
7.803 ± 0.098
6.029 ± 0.061
6.49 ± 0.062
1.535 ± 0.031
2.628 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here