Mycobacterium phage Frankie

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 103 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2L0HJY7|A0A2L0HJY7_9CAUD Uncharacterized protein OS=Mycobacterium phage Frankie OX=2079565 GN=SEA_FRANKIE_89 PE=4 SV=1
MM1 pKa = 8.28PDD3 pKa = 4.48PINYY7 pKa = 10.02DD8 pKa = 3.46EE9 pKa = 5.03TPCALCDD16 pKa = 4.32CYY18 pKa = 11.21GLQHH22 pKa = 7.56DD23 pKa = 4.88DD24 pKa = 4.18PEE26 pKa = 6.27HH27 pKa = 6.52GGPCRR32 pKa = 11.84ALDD35 pKa = 3.54GHH37 pKa = 6.34GRR39 pKa = 11.84PCGCPGFEE47 pKa = 4.28EE48 pKa = 5.39PEE50 pKa = 4.24DD51 pKa = 3.69ATT53 pKa = 4.48

Molecular weight: 5.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2L0HJU3|A0A2L0HJU3_9CAUD Integrase OS=Mycobacterium phage Frankie OX=2079565 GN=SEA_FRANKIE_47 PE=3 SV=1
MM1 pKa = 6.98TEE3 pKa = 3.01VWRR6 pKa = 11.84PVVGQEE12 pKa = 4.07GNYY15 pKa = 9.54EE16 pKa = 3.98VSNLGRR22 pKa = 11.84VRR24 pKa = 11.84SLDD27 pKa = 3.39RR28 pKa = 11.84QIDD31 pKa = 3.28VDD33 pKa = 3.8GRR35 pKa = 11.84GRR37 pKa = 11.84RR38 pKa = 11.84YY39 pKa = 8.99MRR41 pKa = 11.84GRR43 pKa = 11.84VLSLVPMKK51 pKa = 10.7NGYY54 pKa = 9.18RR55 pKa = 11.84LAALPGGKK63 pKa = 9.54KK64 pKa = 9.54LVHH67 pKa = 6.24RR68 pKa = 11.84LVLEE72 pKa = 4.44AFSGPCPPGAEE83 pKa = 4.38GCHH86 pKa = 6.06NDD88 pKa = 3.71GDD90 pKa = 4.04RR91 pKa = 11.84SNNRR95 pKa = 11.84IEE97 pKa = 4.0NLRR100 pKa = 11.84WDD102 pKa = 3.48TRR104 pKa = 11.84TANNIDD110 pKa = 3.79AVDD113 pKa = 4.53HH114 pKa = 5.46RR115 pKa = 11.84TNWQSRR121 pKa = 11.84KK122 pKa = 8.02DD123 pKa = 3.56TCPRR127 pKa = 11.84GHH129 pKa = 6.53VYY131 pKa = 10.8DD132 pKa = 4.42GVSHH136 pKa = 7.04RR137 pKa = 11.84SNGRR141 pKa = 11.84PMRR144 pKa = 11.84YY145 pKa = 9.68CKK147 pKa = 9.69TCRR150 pKa = 11.84NARR153 pKa = 11.84NRR155 pKa = 11.84EE156 pKa = 3.47AWRR159 pKa = 11.84RR160 pKa = 11.84SHH162 pKa = 6.89

Molecular weight: 18.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

103

0

103

17959

30

1191

174.4

18.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.387 ± 0.444

1.158 ± 0.15

6.726 ± 0.232

5.663 ± 0.275

2.801 ± 0.177

8.586 ± 0.507

2.183 ± 0.172

4.46 ± 0.183

3.285 ± 0.182

7.456 ± 0.193

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.244 ± 0.114

3.308 ± 0.199

6.097 ± 0.203

3.447 ± 0.182

6.699 ± 0.381

5.997 ± 0.275

6.298 ± 0.261

7.484 ± 0.316

2.105 ± 0.145

2.617 ± 0.156
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski