Clavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / DSM 20744 / JCM 9667 / LMG 2889 / C-1) (Corynebacterium sepedonicum)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Clavibacter; Clavibacter michiganensis

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3058 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B0RE84|B0RE84_CLAMS Putative integral membrane protein OS=Clavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / DSM 20744 / JCM 9667 / LMG 2889 / C-1) OX=31964 GN=CMS0681 PE=4 SV=1
MM1 pKa = 6.81TTTTFPVTGMTCAHH15 pKa = 6.55CVASVTEE22 pKa = 4.47EE23 pKa = 3.95VGEE26 pKa = 4.23LPGVASVAVDD36 pKa = 3.71LVVGGASTVTVEE48 pKa = 4.7SDD50 pKa = 3.58APLDD54 pKa = 3.88PAALRR59 pKa = 11.84AAVDD63 pKa = 3.34EE64 pKa = 4.6AGYY67 pKa = 10.58VAGLL71 pKa = 3.54

Molecular weight: 6.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B0RAH0|B0RAH0_CLAMS Uncharacterized protein OS=Clavibacter michiganensis subsp. sepedonicus (strain ATCC 33113 / DSM 20744 / JCM 9667 / LMG 2889 / C-1) OX=31964 GN=CMS0215 PE=4 SV=1
MM1 pKa = 7.57PLIRR5 pKa = 11.84FLFVAVPVIRR15 pKa = 11.84KK16 pKa = 7.98FLRR19 pKa = 11.84SRR21 pKa = 11.84QGKK24 pKa = 8.84AALAKK29 pKa = 10.56GKK31 pKa = 10.25AQMAKK36 pKa = 9.99RR37 pKa = 11.84SQKK40 pKa = 10.13RR41 pKa = 11.84AAQRR45 pKa = 11.84TTPTSRR51 pKa = 11.84RR52 pKa = 3.58

Molecular weight: 5.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3057

1

3058

960179

21

2007

314.0

33.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.033 ± 0.081

0.491 ± 0.009

6.516 ± 0.043

5.324 ± 0.04

2.763 ± 0.033

9.178 ± 0.044

2.071 ± 0.026

4.112 ± 0.029

1.748 ± 0.033

9.897 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.739 ± 0.018

1.578 ± 0.021

5.773 ± 0.036

2.665 ± 0.022

7.974 ± 0.056

5.566 ± 0.033

6.084 ± 0.044

9.238 ± 0.045

1.378 ± 0.019

1.873 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski