Pseudooceanicola batsensis (strain ATCC BAA-863 / DSM 15984 / KCTC 12145 / HTCC2597) (Oceanicola batsensis)
Average proteome isoelectric point is 6.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4193 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A3U2A6|A3U2A6_PSEBH Uncharacterized protein OS=Pseudooceanicola batsensis (strain ATCC BAA-863 / DSM 15984 / KCTC 12145 / HTCC2597) OX=252305 GN=OB2597_14721 PE=4 SV=1
MM1 pKa = 7.28 TYY3 pKa = 10.3 YY4 pKa = 10.25 YY5 pKa = 10.86 SKK7 pKa = 9.73 YY8 pKa = 10.4 SYY10 pKa = 10.36 YY11 pKa = 10.69 NKK13 pKa = 10.02 HH14 pKa = 6.22 SYY16 pKa = 9.9 SEE18 pKa = 4.67 KK19 pKa = 9.5 KK20 pKa = 10.56 YY21 pKa = 9.55 EE22 pKa = 3.68 WTAYY26 pKa = 9.56 TEE28 pKa = 4.24 ADD30 pKa = 4.17 LLGDD34 pKa = 4.14 HH35 pKa = 6.7 NGKK38 pKa = 10.62 SIDD41 pKa = 4.1 CGDD44 pKa = 4.1 TFPMPGSATVCMSTYY59 pKa = 11.24 DD60 pKa = 3.41 NDD62 pKa = 4.46 GYY64 pKa = 11.58 LSGDD68 pKa = 3.77 SSWWSDD74 pKa = 3.98 DD75 pKa = 3.22 KK76 pKa = 11.58 ATDD79 pKa = 3.49 STGQKK84 pKa = 10.64 AYY86 pKa = 9.89 IDD88 pKa = 3.86 GGRR91 pKa = 11.84 EE92 pKa = 3.85 GGQLYY97 pKa = 10.59 AEE99 pKa = 4.51 QYY101 pKa = 10.17 HH102 pKa = 5.26 VLKK105 pKa = 10.99 GSDD108 pKa = 2.72 GKK110 pKa = 11.04 YY111 pKa = 9.66 YY112 pKa = 10.4 YY113 pKa = 10.48 LIEE116 pKa = 5.39 IEE118 pKa = 4.3 VEE120 pKa = 4.51 GYY122 pKa = 8.91 DD123 pKa = 4.73 APGKK127 pKa = 10.39 GDD129 pKa = 4.38 DD130 pKa = 3.63 YY131 pKa = 10.77 FTFYY135 pKa = 11.33 GKK137 pKa = 10.21 VPPAGVEE144 pKa = 4.07 LEE146 pKa = 4.41 VVSTKK151 pKa = 10.66 NVWGCMIDD159 pKa = 3.83 YY160 pKa = 9.76 RR161 pKa = 11.84 CLGAGDD167 pKa = 4.18 KK168 pKa = 10.97 APEE171 pKa = 3.92 NTPPEE176 pKa = 4.22 FTNDD180 pKa = 3.06 PKK182 pKa = 11.08 YY183 pKa = 11.24 GKK185 pKa = 9.6 ICIDD189 pKa = 3.57 EE190 pKa = 4.36 NSTFVIDD197 pKa = 5.92 LNAKK201 pKa = 10.32 DD202 pKa = 5.14 DD203 pKa = 4.9 DD204 pKa = 5.09 GDD206 pKa = 3.77 TLTYY210 pKa = 10.19 EE211 pKa = 4.12 IVGGEE216 pKa = 4.04 DD217 pKa = 3.24 ADD219 pKa = 3.8 KK220 pKa = 11.4 FEE222 pKa = 5.83 IDD224 pKa = 3.76 AEE226 pKa = 4.31 TGEE229 pKa = 5.15 LRR231 pKa = 11.84 FKK233 pKa = 10.7 DD234 pKa = 3.5 APDD237 pKa = 3.61 YY238 pKa = 10.46 EE239 pKa = 4.33 NPTDD243 pKa = 3.58 KK244 pKa = 11.67 GEE246 pKa = 4.49 DD247 pKa = 2.99 NVYY250 pKa = 9.43 EE251 pKa = 4.36 VKK253 pKa = 11.08 VKK255 pKa = 10.93 VSDD258 pKa = 3.91 GNGGEE263 pKa = 4.35 DD264 pKa = 3.28 TTKK267 pKa = 10.99 LKK269 pKa = 11.1 VCVEE273 pKa = 4.21 DD274 pKa = 5.08 VEE276 pKa = 4.48 EE277 pKa = 4.05 QTGKK281 pKa = 10.79 CIVIEE286 pKa = 4.29 AEE288 pKa = 5.56 DD289 pKa = 3.47 MCEE292 pKa = 3.73 WGFSKK297 pKa = 10.54 AWGCDD302 pKa = 3.01 ASGGKK307 pKa = 8.94 LVKK310 pKa = 10.36 LNCAGGQGKK319 pKa = 9.15 LWTTFDD325 pKa = 4.32 GPSGTYY331 pKa = 9.97 DD332 pKa = 3.39 FKK334 pKa = 11.13 IYY336 pKa = 10.67 AQDD339 pKa = 3.66 EE340 pKa = 4.26 NDD342 pKa = 3.94 GQSKK346 pKa = 10.34 LKK348 pKa = 10.35 IYY350 pKa = 10.64 INGEE354 pKa = 3.55 FKK356 pKa = 10.83 GYY358 pKa = 10.25 IKK360 pKa = 10.66 LDD362 pKa = 3.75 RR363 pKa = 11.84 DD364 pKa = 3.6 SDD366 pKa = 4.2 GSGSNHH372 pKa = 5.05 GTFSTFVLEE381 pKa = 5.51 DD382 pKa = 3.22 IEE384 pKa = 4.45 INKK387 pKa = 10.01 GDD389 pKa = 4.04 TIKK392 pKa = 10.39 IYY394 pKa = 11.12 ADD396 pKa = 3.73 GDD398 pKa = 3.81 CNEE401 pKa = 4.25 YY402 pKa = 10.74 VRR404 pKa = 11.84 IDD406 pKa = 3.95 KK407 pKa = 10.79 IEE409 pKa = 3.96 LCKK412 pKa = 10.39 DD413 pKa = 3.68 DD414 pKa = 5.18 KK415 pKa = 11.23 PLGAIGDD422 pKa = 4.4 TVWYY426 pKa = 8.3 DD427 pKa = 3.24 TDD429 pKa = 4.31 RR430 pKa = 11.84 DD431 pKa = 4.06 GVQDD435 pKa = 3.98 DD436 pKa = 4.67 GEE438 pKa = 5.62 AGVEE442 pKa = 3.96 NVTVNLLNGAGVVIATQMTDD462 pKa = 2.77 VNGNYY467 pKa = 10.19 LFEE470 pKa = 4.29 EE471 pKa = 4.91 LEE473 pKa = 4.18 AGDD476 pKa = 3.71 YY477 pKa = 10.39 KK478 pKa = 11.41 VEE480 pKa = 4.35 FEE482 pKa = 4.79 LPDD485 pKa = 3.65 GNFVFTTQDD494 pKa = 2.99 AGSDD498 pKa = 3.69 DD499 pKa = 5.24 AVDD502 pKa = 3.86 SDD504 pKa = 4.87 ADD506 pKa = 3.95 TTTGLTDD513 pKa = 4.08 TIALGEE519 pKa = 4.5 GEE521 pKa = 4.53 TNLTVDD527 pKa = 4.42 AGLYY531 pKa = 10.27 DD532 pKa = 4.65 PNDD535 pKa = 3.82 HH536 pKa = 7.55 PEE538 pKa = 4.23 PTDD541 pKa = 4.26 DD542 pKa = 5.03 AAKK545 pKa = 9.4 TCADD549 pKa = 3.59 EE550 pKa = 4.55 EE551 pKa = 4.54 KK552 pKa = 10.28 TVDD555 pKa = 3.62 VLANDD560 pKa = 3.87 SDD562 pKa = 4.66 PNGDD566 pKa = 3.56 TLTITHH572 pKa = 6.58 VDD574 pKa = 3.41 GQAITEE580 pKa = 4.31 GGEE583 pKa = 4.21 VTTSAGTLVKK593 pKa = 10.61 LLGGMLVVDD602 pKa = 5.04 GEE604 pKa = 4.36 DD605 pKa = 3.26 AYY607 pKa = 10.77 EE608 pKa = 4.38 DD609 pKa = 4.07 LDD611 pKa = 3.87 IAEE614 pKa = 5.08 HH615 pKa = 6.49 AMEE618 pKa = 5.73 DD619 pKa = 2.92 ISYY622 pKa = 8.82 TVSDD626 pKa = 4.02 GMGGSATANLEE637 pKa = 3.95 MTFCGDD643 pKa = 3.23 ANTVEE648 pKa = 4.56 SFVGYY653 pKa = 9.12 MPEE656 pKa = 4.01 TVSYY660 pKa = 10.49 KK661 pKa = 10.56 VADD664 pKa = 4.0 GLRR667 pKa = 11.84 NDD669 pKa = 3.84 PYY671 pKa = 11.27 EE672 pKa = 4.36 DD673 pKa = 2.95 VGYY676 pKa = 10.43 DD677 pKa = 3.36 ILITEE682 pKa = 4.42 SDD684 pKa = 3.34 NGRR687 pKa = 11.84 LDD689 pKa = 3.61 GVEE692 pKa = 4.57 FTAAYY697 pKa = 10.25 CLDD700 pKa = 3.88 FNRR703 pKa = 11.84 SITTGADD710 pKa = 3.02 YY711 pKa = 11.21 DD712 pKa = 4.06 SAAEE716 pKa = 3.98 LDD718 pKa = 3.11 GDD720 pKa = 3.59 MFVFNDD726 pKa = 3.62 DD727 pKa = 3.57 TASSVLLASQNGVNGAALDD746 pKa = 3.94 NVNEE750 pKa = 4.4 INWILNQDD758 pKa = 3.9 FEE760 pKa = 5.59 SDD762 pKa = 3.41 GTGSVDD768 pKa = 3.33 GKK770 pKa = 10.28 FSGWEE775 pKa = 3.52 VQYY778 pKa = 10.94 AIWEE782 pKa = 4.26 LTNNFPSDD790 pKa = 3.29 AALNATPSNGQVEE803 pKa = 4.61 DD804 pKa = 2.8 VDD806 pKa = 4.88 YY807 pKa = 10.75 IVSRR811 pKa = 11.84 AISEE815 pKa = 4.66 GYY817 pKa = 10.39 DD818 pKa = 3.35 FEE820 pKa = 7.23 AGVGDD825 pKa = 4.54 IIGLVITPDD834 pKa = 3.23 PVTAQNAQPFLVGVEE849 pKa = 4.09 FEE851 pKa = 5.22 TYY853 pKa = 10.71 DD854 pKa = 3.8 CLCC857 pKa = 4.56
Molecular weight: 92.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.707
IPC2_protein 3.732
IPC_protein 3.783
Toseland 3.554
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.694
Rodwell 3.592
Grimsley 3.452
Solomon 3.77
Lehninger 3.719
Nozaki 3.872
DTASelect 4.126
Thurlkill 3.592
EMBOSS 3.706
Sillero 3.897
Patrickios 0.833
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.795
Protein with the highest isoelectric point:
>tr|A3TXB8|A3TXB8_PSEBH Uncharacterized protein OS=Pseudooceanicola batsensis (strain ATCC BAA-863 / DSM 15984 / KCTC 12145 / HTCC2597) OX=252305 GN=OB2597_02622 PE=4 SV=1
MM1 pKa = 7.61 ILRR4 pKa = 11.84 RR5 pKa = 11.84 VLSRR9 pKa = 11.84 GLKK12 pKa = 10.02 AGMGRR17 pKa = 11.84 MAKK20 pKa = 10.32 GDD22 pKa = 3.65 GQGPQSKK29 pKa = 9.75 RR30 pKa = 11.84 ARR32 pKa = 11.84 QTARR36 pKa = 11.84 RR37 pKa = 11.84 ARR39 pKa = 11.84 KK40 pKa = 8.67 AARR43 pKa = 11.84 LMRR46 pKa = 11.84 RR47 pKa = 11.84 NGRR50 pKa = 11.84 LL51 pKa = 3.09
Molecular weight: 5.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.455
IPC2_protein 10.979
IPC_protein 12.574
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.217
Rodwell 12.369
Grimsley 12.778
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.106
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.119
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4193
0
4193
1317978
19
12228
314.3
34.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.325 ± 0.062
0.836 ± 0.018
6.337 ± 0.07
6.265 ± 0.033
3.618 ± 0.028
9.036 ± 0.055
2.025 ± 0.026
5.02 ± 0.028
2.706 ± 0.038
9.891 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.712 ± 0.027
2.407 ± 0.025
5.175 ± 0.034
2.953 ± 0.02
7.332 ± 0.058
5.0 ± 0.028
5.521 ± 0.045
7.334 ± 0.03
1.319 ± 0.016
2.189 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here