Amino acid dipepetide frequency for Eubacterium sp. CAG:161

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.698AlaAla: 4.698 ± 0.098	0.772AlaCys: 0.772 ± 0.039	3.909AlaAsp: 3.909 ± 0.082	3.872AlaGlu: 3.872 ± 0.077	2.656AlaPhe: 2.656 ± 0.067	5.012AlaGly: 5.012 ± 0.091	0.873AlaHis: 0.873 ± 0.038	5.348AlaIle: 5.348 ± 0.098	5.458AlaLys: 5.458 ± 0.103	5.451AlaLeu: 5.451 ± 0.11	2.073AlaMet: 2.073 ± 0.06	2.849AlaAsn: 2.849 ± 0.063	1.7AlaPro: 1.7 ± 0.059	1.698AlaGln: 1.698 ± 0.053	2.061AlaArg: 2.061 ± 0.046	3.409AlaSer: 3.409 ± 0.076	3.647AlaThr: 3.647 ± 0.088	5.185AlaVal: 5.185 ± 0.094	0.457AlaTrp: 0.457 ± 0.029	2.707AlaTyr: 2.707 ± 0.061	0.003AlaXaa: 0.003 ± 0.002
Cys	0.769CysAla: 0.769 ± 0.032	0.24CysCys: 0.24 ± 0.022	0.844CysAsp: 0.844 ± 0.033	0.761CysGlu: 0.761 ± 0.037	0.598CysPhe: 0.598 ± 0.028	1.348CysGly: 1.348 ± 0.058	0.259CysHis: 0.259 ± 0.019	1.259CysIle: 1.259 ± 0.045	0.963CysLys: 0.963 ± 0.04	0.957CysLeu: 0.957 ± 0.037	0.405CysMet: 0.405 ± 0.022	0.711CysAsn: 0.711 ± 0.035	0.512CysPro: 0.512 ± 0.033	0.309CysGln: 0.309 ± 0.02	0.453CysArg: 0.453 ± 0.024	0.879CysSer: 0.879 ± 0.039	0.639CysThr: 0.639 ± 0.029	0.998CysVal: 0.998 ± 0.037	0.094CysTrp: 0.094 ± 0.013	0.585CysTyr: 0.585 ± 0.027	0.001CysXaa: 0.001 ± 0.001
Asp	3.502AspAla: 3.502 ± 0.075	0.713AspCys: 0.713 ± 0.032	3.654AspAsp: 3.654 ± 0.08	4.898AspGlu: 4.898 ± 0.094	2.82AspPhe: 2.82 ± 0.063	4.483AspGly: 4.483 ± 0.093	0.618AspHis: 0.618 ± 0.034	5.515AspIle: 5.515 ± 0.094	5.278AspLys: 5.278 ± 0.092	4.202AspLeu: 4.202 ± 0.075	1.959AspMet: 1.959 ± 0.058	3.736AspAsn: 3.736 ± 0.091	1.232AspPro: 1.232 ± 0.049	0.825AspGln: 0.825 ± 0.035	2.07AspArg: 2.07 ± 0.059	3.453AspSer: 3.453 ± 0.083	3.376AspThr: 3.376 ± 0.091	4.183AspVal: 4.183 ± 0.083	0.512AspTrp: 0.512 ± 0.031	3.263AspTyr: 3.263 ± 0.069	0.001AspXaa: 0.001 ± 0.001
Glu	4.501GluAla: 4.501 ± 0.09	0.843GluCys: 0.843 ± 0.04	4.134GluAsp: 4.134 ± 0.097	5.856GluGlu: 5.856 ± 0.125	2.547GluPhe: 2.547 ± 0.063	3.897GluGly: 3.897 ± 0.081	1.072GluHis: 1.072 ± 0.043	6.129GluIle: 6.129 ± 0.111	7.378GluLys: 7.378 ± 0.126	5.619GluLeu: 5.619 ± 0.092	2.233GluMet: 2.233 ± 0.058	5.254GluAsn: 5.254 ± 0.097	1.553GluPro: 1.553 ± 0.054	1.988GluGln: 1.988 ± 0.063	2.447GluArg: 2.447 ± 0.061	3.252GluSer: 3.252 ± 0.07	3.555GluThr: 3.555 ± 0.08	4.381GluVal: 4.381 ± 0.083	0.535GluTrp: 0.535 ± 0.029	3.439GluTyr: 3.439 ± 0.082	0.001GluXaa: 0.001 ± 0.001
Phe	2.524PheAla: 2.524 ± 0.059	0.567PheCys: 0.567 ± 0.031	2.723PheAsp: 2.723 ± 0.059	2.717PheGlu: 2.717 ± 0.063	1.738PhePhe: 1.738 ± 0.059	2.945PheGly: 2.945 ± 0.08	0.604PheHis: 0.604 ± 0.031	3.143PheIle: 3.143 ± 0.083	2.983PheLys: 2.983 ± 0.06	2.967PheLeu: 2.967 ± 0.078	1.228PheMet: 1.228 ± 0.047	2.282PheAsn: 2.282 ± 0.057	1.105PhePro: 1.105 ± 0.04	0.897PheGln: 0.897 ± 0.039	1.316PheArg: 1.316 ± 0.042	2.979PheSer: 2.979 ± 0.071	2.296PheThr: 2.296 ± 0.062	3.01PheVal: 3.01 ± 0.068	0.333PheTrp: 0.333 ± 0.025	1.784PheTyr: 1.784 ± 0.045	0.0PheXaa: 0.0 ± 0.0
Gly	4.425GlyAla: 4.425 ± 0.097	1.104GlyCys: 1.104 ± 0.045	3.76GlyAsp: 3.76 ± 0.076	4.45GlyGlu: 4.45 ± 0.086	2.835GlyPhe: 2.835 ± 0.061	4.356GlyGly: 4.356 ± 0.086	1.149GlyHis: 1.149 ± 0.042	6.431GlyIle: 6.431 ± 0.109	6.574GlyLys: 6.574 ± 0.112	4.956GlyLeu: 4.956 ± 0.09	2.217GlyMet: 2.217 ± 0.058	3.785GlyAsn: 3.785 ± 0.078	1.091GlyPro: 1.091 ± 0.041	1.795GlyGln: 1.795 ± 0.058	2.502GlyArg: 2.502 ± 0.06	3.767GlySer: 3.767 ± 0.085	4.201GlyThr: 4.201 ± 0.104	4.874GlyVal: 4.874 ± 0.086	0.643GlyTrp: 0.643 ± 0.038	3.514GlyTyr: 3.514 ± 0.073	0.0GlyXaa: 0.0 ± 0.0
His	0.747HisAla: 0.747 ± 0.03	0.269HisCys: 0.269 ± 0.017	0.685HisAsp: 0.685 ± 0.033	0.862HisGlu: 0.862 ± 0.033	0.706HisPhe: 0.706 ± 0.033	1.058HisGly: 1.058 ± 0.042	0.331HisHis: 0.331 ± 0.033	1.282HisIle: 1.282 ± 0.048	1.044HisLys: 1.044 ± 0.044	1.058HisLeu: 1.058 ± 0.039	0.421HisMet: 0.421 ± 0.024	0.847HisAsn: 0.847 ± 0.033	0.585HisPro: 0.585 ± 0.035	0.405HisGln: 0.405 ± 0.023	0.596HisArg: 0.596 ± 0.032	0.855HisSer: 0.855 ± 0.037	0.804HisThr: 0.804 ± 0.033	0.891HisVal: 0.891 ± 0.04	0.152HisTrp: 0.152 ± 0.014	0.629HisTyr: 0.629 ± 0.031	0.001HisXaa: 0.001 ± 0.001
Ile	5.555IleAla: 5.555 ± 0.096	1.36IleCys: 1.36 ± 0.048	4.962IleAsp: 4.962 ± 0.095	5.47IleGlu: 5.47 ± 0.107	3.279IlePhe: 3.279 ± 0.097	5.276IleGly: 5.276 ± 0.082	1.108IleHis: 1.108 ± 0.045	7.141IleIle: 7.141 ± 0.152	7.242IleLys: 7.242 ± 0.113	6.593IleLeu: 6.593 ± 0.11	2.425IleMet: 2.425 ± 0.07	5.046IleAsn: 5.046 ± 0.089	2.904IlePro: 2.904 ± 0.065	1.91IleGln: 1.91 ± 0.049	2.893IleArg: 2.893 ± 0.073	5.823IleSer: 5.823 ± 0.107	4.994IleThr: 4.994 ± 0.082	5.958IleVal: 5.958 ± 0.103	0.598IleTrp: 0.598 ± 0.032	3.614IleTyr: 3.614 ± 0.091	0.0IleXaa: 0.0 ± 0.0
Lys	5.501LysAla: 5.501 ± 0.098	1.038LysCys: 1.038 ± 0.04	5.253LysAsp: 5.253 ± 0.09	7.313LysGlu: 7.313 ± 0.131	2.689LysPhe: 2.689 ± 0.062	5.156LysGly: 5.156 ± 0.1	1.119LysHis: 1.119 ± 0.04	7.209LysIle: 7.209 ± 0.11	9.092LysLys: 9.092 ± 0.177	6.312LysLeu: 6.312 ± 0.089	2.852LysMet: 2.852 ± 0.062	5.709LysAsn: 5.709 ± 0.089	2.22LysPro: 2.22 ± 0.057	2.272LysGln: 2.272 ± 0.058	3.111LysArg: 3.111 ± 0.07	4.786LysSer: 4.786 ± 0.1	4.683LysThr: 4.683 ± 0.075	6.22LysVal: 6.22 ± 0.114	0.811LysTrp: 0.811 ± 0.034	4.456LysTyr: 4.456 ± 0.076	0.0LysXaa: 0.0 ± 0.0
Leu	5.07LeuAla: 5.07 ± 0.078	1.231LeuCys: 1.231 ± 0.047	4.68LeuAsp: 4.68 ± 0.089	5.175LeuGlu: 5.175 ± 0.088	3.211LeuPhe: 3.211 ± 0.093	5.102LeuGly: 5.102 ± 0.095	1.127LeuHis: 1.127 ± 0.044	6.073LeuIle: 6.073 ± 0.119	6.857LeuLys: 6.857 ± 0.098	6.37LeuLeu: 6.37 ± 0.141	2.238LeuMet: 2.238 ± 0.059	4.241LeuAsn: 4.241 ± 0.087	2.648LeuPro: 2.648 ± 0.068	1.963LeuGln: 1.963 ± 0.056	2.681LeuArg: 2.681 ± 0.06	5.543LeuSer: 5.543 ± 0.102	4.196LeuThr: 4.196 ± 0.077	4.984LeuVal: 4.984 ± 0.085	0.631LeuTrp: 0.631 ± 0.033	3.277LeuTyr: 3.277 ± 0.069	0.001LeuXaa: 0.001 ± 0.002
Met	2.394MetAla: 2.394 ± 0.065	0.398MetCys: 0.398 ± 0.027	1.894MetAsp: 1.894 ± 0.048	2.325MetGlu: 2.325 ± 0.063	1.18MetPhe: 1.18 ± 0.04	1.982MetGly: 1.982 ± 0.059	0.385MetHis: 0.385 ± 0.024	2.271MetIle: 2.271 ± 0.066	2.613MetLys: 2.613 ± 0.057	2.386MetLeu: 2.386 ± 0.059	0.794MetMet: 0.794 ± 0.037	1.583MetAsn: 1.583 ± 0.043	1.012MetPro: 1.012 ± 0.031	0.8MetGln: 0.8 ± 0.037	1.077MetArg: 1.077 ± 0.043	1.924MetSer: 1.924 ± 0.057	1.6MetThr: 1.6 ± 0.053	2.012MetVal: 2.012 ± 0.056	0.223MetTrp: 0.223 ± 0.019	1.149MetTyr: 1.149 ± 0.043	0.0MetXaa: 0.0 ± 0.0
Asn	3.309AsnAla: 3.309 ± 0.071	0.747AsnCys: 0.747 ± 0.035	3.032AsnAsp: 3.032 ± 0.085	3.879AsnGlu: 3.879 ± 0.068	1.924AsnPhe: 1.924 ± 0.05	4.506AsnGly: 4.506 ± 0.108	0.843AsnHis: 0.843 ± 0.041	5.562AsnIle: 5.562 ± 0.086	4.847AsnLys: 4.847 ± 0.09	4.094AsnLeu: 4.094 ± 0.085	1.765AsnMet: 1.765 ± 0.051	3.711AsnAsn: 3.711 ± 0.086	2.091AsnPro: 2.091 ± 0.055	1.575AsnGln: 1.575 ± 0.044	2.196AsnArg: 2.196 ± 0.064	3.455AsnSer: 3.455 ± 0.083	3.128AsnThr: 3.128 ± 0.072	4.199AsnVal: 4.199 ± 0.088	0.53AsnTrp: 0.53 ± 0.031	2.563AsnTyr: 2.563 ± 0.069	0.001AsnXaa: 0.001 ± 0.001
Pro	1.737ProAla: 1.737 ± 0.053	0.37ProCys: 0.37 ± 0.022	2.088ProAsp: 2.088 ± 0.059	2.567ProGlu: 2.567 ± 0.069	1.221ProPhe: 1.221 ± 0.04	1.981ProGly: 1.981 ± 0.064	0.466ProHis: 0.466 ± 0.027	2.048ProIle: 2.048 ± 0.055	2.071ProLys: 2.071 ± 0.05	2.135ProLeu: 2.135 ± 0.063	0.715ProMet: 0.715 ± 0.027	1.246ProAsn: 1.246 ± 0.045	0.512ProPro: 0.512 ± 0.03	0.736ProGln: 0.736 ± 0.035	0.71ProArg: 0.71 ± 0.037	1.525ProSer: 1.525 ± 0.05	1.634ProThr: 1.634 ± 0.061	2.529ProVal: 2.529 ± 0.065	0.252ProTrp: 0.252 ± 0.02	1.397ProTyr: 1.397 ± 0.046	0.003ProXaa: 0.003 ± 0.002
Gln	1.598GlnAla: 1.598 ± 0.053	0.322GlnCys: 0.322 ± 0.018	1.253GlnAsp: 1.253 ± 0.045	1.668GlnGlu: 1.668 ± 0.043	1.047GlnPhe: 1.047 ± 0.037	1.607GlnGly: 1.607 ± 0.049	0.347GlnHis: 0.347 ± 0.024	2.276GlnIle: 2.276 ± 0.056	2.322GlnLys: 2.322 ± 0.054	2.203GlnLeu: 2.203 ± 0.053	0.841GlnMet: 0.841 ± 0.036	1.443GlnAsn: 1.443 ± 0.049	0.663GlnPro: 0.663 ± 0.034	0.894GlnGln: 0.894 ± 0.044	0.925GlnArg: 0.925 ± 0.04	1.357GlnSer: 1.357 ± 0.046	1.235GlnThr: 1.235 ± 0.038	1.756GlnVal: 1.756 ± 0.053	0.293GlnTrp: 0.293 ± 0.022	1.181GlnTyr: 1.181 ± 0.04	0.0GlnXaa: 0.0 ± 0.0
Arg	1.974ArgAla: 1.974 ± 0.05	0.444ArgCys: 0.444 ± 0.024	2.041ArgAsp: 2.041 ± 0.061	2.834ArgGlu: 2.834 ± 0.07	1.467ArgPhe: 1.467 ± 0.052	2.027ArgGly: 2.027 ± 0.068	0.586ArgHis: 0.586 ± 0.031	2.985ArgIle: 2.985 ± 0.067	3.415ArgLys: 3.415 ± 0.069	2.688ArgLeu: 2.688 ± 0.061	1.133ArgMet: 1.133 ± 0.039	2.164ArgAsn: 2.164 ± 0.066	0.916ArgPro: 0.916 ± 0.042	1.144ArgGln: 1.144 ± 0.04	1.524ArgArg: 1.524 ± 0.058	1.596ArgSer: 1.596 ± 0.049	1.754ArgThr: 1.754 ± 0.048	2.333ArgVal: 2.333 ± 0.064	0.254ArgTrp: 0.254 ± 0.018	1.683ArgTyr: 1.683 ± 0.051	0.0ArgXaa: 0.0 ± 0.0
Ser	3.732SerAla: 3.732 ± 0.076	0.682SerCys: 0.682 ± 0.032	4.101SerAsp: 4.101 ± 0.089	3.965SerGlu: 3.965 ± 0.079	2.604SerPhe: 2.604 ± 0.058	4.829SerGly: 4.829 ± 0.1	0.879SerHis: 0.879 ± 0.03	4.497SerIle: 4.497 ± 0.087	4.96SerLys: 4.96 ± 0.106	4.705SerLeu: 4.705 ± 0.091	1.636SerMet: 1.636 ± 0.046	3.211SerAsn: 3.211 ± 0.073	1.481SerPro: 1.481 ± 0.044	1.701SerGln: 1.701 ± 0.043	2.224SerArg: 2.224 ± 0.057	3.775SerSer: 3.775 ± 0.081	3.168SerThr: 3.168 ± 0.08	4.68SerVal: 4.68 ± 0.081	0.55SerTrp: 0.55 ± 0.031	2.82SerTyr: 2.82 ± 0.075	0.003SerXaa: 0.003 ± 0.002
Thr	3.653ThrAla: 3.653 ± 0.08	0.593ThrCys: 0.593 ± 0.031	3.437ThrAsp: 3.437 ± 0.089	3.535ThrGlu: 3.535 ± 0.069	2.262ThrPhe: 2.262 ± 0.059	4.567ThrGly: 4.567 ± 0.095	0.746ThrHis: 0.746 ± 0.034	4.686ThrIle: 4.686 ± 0.086	4.245ThrLys: 4.245 ± 0.079	4.46ThrLeu: 4.46 ± 0.077	1.479ThrMet: 1.479 ± 0.049	2.663ThrAsn: 2.663 ± 0.08	2.014ThrPro: 2.014 ± 0.049	1.296ThrGln: 1.296 ± 0.045	1.69ThrArg: 1.69 ± 0.044	3.294ThrSer: 3.294 ± 0.08	3.614ThrThr: 3.614 ± 0.109	4.819ThrVal: 4.819 ± 0.109	0.498ThrTrp: 0.498 ± 0.026	2.628ThrTyr: 2.628 ± 0.074	0.001ThrXaa: 0.001 ± 0.001
Val	4.984ValAla: 4.984 ± 0.094	1.092ValCys: 1.092 ± 0.04	4.223ValAsp: 4.223 ± 0.082	4.822ValGlu: 4.822 ± 0.081	2.994ValPhe: 2.994 ± 0.073	4.356ValGly: 4.356 ± 0.083	0.878ValHis: 0.878 ± 0.038	5.965ValIle: 5.965 ± 0.104	6.15ValLys: 6.15 ± 0.116	5.983ValLeu: 5.983 ± 0.104	2.074ValMet: 2.074 ± 0.062	3.86ValAsn: 3.86 ± 0.086	2.217ValPro: 2.217 ± 0.062	1.543ValGln: 1.543 ± 0.045	2.432ValArg: 2.432 ± 0.066	4.838ValSer: 4.838 ± 0.078	4.499ValThr: 4.499 ± 0.1	5.365ValVal: 5.365 ± 0.099	0.595ValTrp: 0.595 ± 0.031	3.171ValTyr: 3.171 ± 0.074	0.003ValXaa: 0.003 ± 0.002
Trp	0.489TrpAla: 0.489 ± 0.03	0.129TrpCys: 0.129 ± 0.015	0.516TrpAsp: 0.516 ± 0.028	0.506TrpGlu: 0.506 ± 0.031	0.391TrpPhe: 0.391 ± 0.025	0.646TrpGly: 0.646 ± 0.037	0.164TrpHis: 0.164 ± 0.016	0.668TrpIle: 0.668 ± 0.032	0.652TrpLys: 0.652 ± 0.033	0.661TrpLeu: 0.661 ± 0.028	0.262TrpMet: 0.262 ± 0.02	0.593TrpAsn: 0.593 ± 0.031	0.205TrpPro: 0.205 ± 0.019	0.299TrpGln: 0.299 ± 0.021	0.261TrpArg: 0.261 ± 0.019	0.584TrpSer: 0.584 ± 0.029	0.478TrpThr: 0.478 ± 0.031	0.494TrpVal: 0.494 ± 0.028	0.1TrpTrp: 0.1 ± 0.012	0.345TrpTyr: 0.345 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.674TyrAla: 2.674 ± 0.064	0.671TyrCys: 0.671 ± 0.028	3.105TyrAsp: 3.105 ± 0.072	3.139TyrGlu: 3.139 ± 0.075	1.973TyrPhe: 1.973 ± 0.046	3.297TyrGly: 3.297 ± 0.069	0.674TyrHis: 0.674 ± 0.036	3.66TyrIle: 3.66 ± 0.075	3.659TyrLys: 3.659 ± 0.071	3.496TyrLeu: 3.496 ± 0.079	1.252TyrMet: 1.252 ± 0.043	3.019TyrAsn: 3.019 ± 0.077	1.285TyrPro: 1.285 ± 0.048	1.178TyrGln: 1.178 ± 0.045	1.79TyrArg: 1.79 ± 0.056	3.105TyrSer: 3.105 ± 0.074	2.68TyrThr: 2.68 ± 0.077	3.171TyrVal: 3.171 ± 0.08	0.383TyrTrp: 0.383 ± 0.026	2.409TyrTyr: 2.409 ± 0.07	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.003XaaHis: 0.003 ± 0.002	0.003XaaIle: 0.003 ± 0.002	0.0XaaLys: 0.0 ± 0.0	0.003XaaLeu: 0.003 ± 0.002	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.001	0.0XaaPro: 0.0 ± 0.0	0.001XaaGln: 0.001 ± 0.001	0.001XaaArg: 0.001 ± 0.002	0.003XaaSer: 0.003 ± 0.002	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.019XaaXaa: 0.019 ± 0.007

Statistics based on 2196 proteins (721333 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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