Planctomyces sp. SH-PL14
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6497 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A142X417|A0A142X417_9PLAN Uncharacterized protein OS=Planctomyces sp. SH-PL14 OX=1632864 GN=VT03_27880 PE=4 SV=1
MM1 pKa = 7.91 RR2 pKa = 11.84 YY3 pKa = 8.64 FVAMAAVVTAVQLAAGVASAQDD25 pKa = 3.6 LASPDD30 pKa = 3.58 EE31 pKa = 4.16 RR32 pKa = 11.84 VEE34 pKa = 3.97 MYY36 pKa = 10.51 EE37 pKa = 4.1 PEE39 pKa = 5.15 DD40 pKa = 3.82 YY41 pKa = 11.23 ASLRR45 pKa = 11.84 ARR47 pKa = 11.84 IEE49 pKa = 3.8 QLEE52 pKa = 3.99 ANQRR56 pKa = 11.84 YY57 pKa = 8.37 SAPAVASLDD66 pKa = 3.87 PQGGVYY72 pKa = 9.69 VQYY75 pKa = 11.26 DD76 pKa = 3.62 NVIVKK81 pKa = 9.77 PYY83 pKa = 10.2 LSEE86 pKa = 4.15 NSAFPAQIADD96 pKa = 3.8 GNVVNYY102 pKa = 9.74 GFNLDD107 pKa = 3.59 MEE109 pKa = 4.55 YY110 pKa = 10.71 SPRR113 pKa = 11.84 IEE115 pKa = 4.26 VGYY118 pKa = 8.01 MAPTSNLGFRR128 pKa = 11.84 ARR130 pKa = 11.84 YY131 pKa = 6.65 WHH133 pKa = 6.72 FGANAATGPANDD145 pKa = 4.34 ANGLIADD152 pKa = 4.32 DD153 pKa = 5.54 AIAIAAGATIQDD165 pKa = 3.43 VDD167 pKa = 4.08 TITTVVYY174 pKa = 10.31 DD175 pKa = 4.18 LNMDD179 pKa = 3.93 VLDD182 pKa = 4.65 LEE184 pKa = 4.42 ATRR187 pKa = 11.84 AVGSSGTFAAGIRR200 pKa = 11.84 TAWMGQKK207 pKa = 10.33 YY208 pKa = 9.96 GLDD211 pKa = 3.47 SDD213 pKa = 4.17 EE214 pKa = 4.81 GQLFTDD220 pKa = 4.72 LDD222 pKa = 3.87 VAAIGPTLAYY232 pKa = 9.0 EE233 pKa = 4.45 WKK235 pKa = 10.46 SHH237 pKa = 6.56 DD238 pKa = 3.35 FWFDD242 pKa = 3.12 GLRR245 pKa = 11.84 GLATVRR251 pKa = 11.84 GSLLYY256 pKa = 10.49 GGKK259 pKa = 10.08 DD260 pKa = 3.28 INSVGPDD267 pKa = 2.95 NTYY270 pKa = 10.26 TYY272 pKa = 10.89 NEE274 pKa = 3.79 QSLISNADD282 pKa = 3.31 LQLGLEE288 pKa = 4.27 YY289 pKa = 10.29 RR290 pKa = 11.84 RR291 pKa = 11.84 SGGFFLRR298 pKa = 11.84 AALEE302 pKa = 3.92 AQYY305 pKa = 9.63 WANTGNATNHH315 pKa = 5.54 NVGPGDD321 pKa = 3.86 DD322 pKa = 3.91 VGDD325 pKa = 4.07 SEE327 pKa = 5.08 FVGSILDD334 pKa = 4.11 DD335 pKa = 4.15 DD336 pKa = 5.07 LGFLGMVFSAGYY348 pKa = 9.32 SWW350 pKa = 3.81
Molecular weight: 37.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.793
IPC2_protein 3.999
IPC_protein 4.012
Toseland 3.795
ProMoST 4.164
Dawson 3.999
Bjellqvist 4.151
Wikipedia 3.935
Rodwell 3.834
Grimsley 3.706
Solomon 3.999
Lehninger 3.948
Nozaki 4.113
DTASelect 4.368
Thurlkill 3.846
EMBOSS 3.948
Sillero 4.139
Patrickios 1.939
IPC_peptide 3.999
IPC2_peptide 4.113
IPC2.peptide.svr19 4.006
Protein with the highest isoelectric point:
>tr|A0A142X5Y7|A0A142X5Y7_9PLAN Circadian clock protein KaiB OS=Planctomyces sp. SH-PL14 OX=1632864 GN=kaiB PE=4 SV=1
MM1 pKa = 7.66 SKK3 pKa = 10.55 RR4 pKa = 11.84 SGGPARR10 pKa = 11.84 RR11 pKa = 11.84 PATIKK16 pKa = 10.79 PNLQKK21 pKa = 11.03 KK22 pKa = 8.34 MRR24 pKa = 11.84 SQLRR28 pKa = 11.84 PLKK31 pKa = 9.58 TVSSKK36 pKa = 11.08 SKK38 pKa = 9.91 SGKK41 pKa = 9.78 SGTRR45 pKa = 3.18
Molecular weight: 4.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 10.994
IPC_protein 12.544
Toseland 12.72
ProMoST 13.217
Dawson 12.72
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.618
Grimsley 12.764
Solomon 13.203
Lehninger 13.115
Nozaki 12.72
DTASelect 12.705
Thurlkill 12.72
EMBOSS 13.217
Sillero 12.72
Patrickios 12.34
IPC_peptide 13.217
IPC2_peptide 12.193
IPC2.peptide.svr19 9.027
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6497
0
6497
2309488
29
4696
355.5
38.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.74 ± 0.033
1.084 ± 0.012
5.602 ± 0.023
6.256 ± 0.025
3.606 ± 0.015
8.226 ± 0.032
2.109 ± 0.016
4.445 ± 0.022
3.442 ± 0.027
10.082 ± 0.033
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.024 ± 0.013
2.608 ± 0.019
6.05 ± 0.032
3.584 ± 0.019
7.555 ± 0.037
5.831 ± 0.022
5.541 ± 0.031
7.381 ± 0.025
1.609 ± 0.015
2.225 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here