Formosa sp. Hel3_A1_48

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Formosa; unclassified Formosa

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1866 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D7XR70|A0A1D7XR70_9FLAO Uncharacterized protein OS=Formosa sp. Hel3_A1_48 OX=1336795 GN=FORMA_06200 PE=4 SV=1
MM1 pKa = 7.66KK2 pKa = 10.48KK3 pKa = 10.62LILLTLTFCYY13 pKa = 10.26SINSFSQVGIGTAEE27 pKa = 4.15PNASSQLEE35 pKa = 4.24VKK37 pKa = 10.66ADD39 pKa = 3.83DD40 pKa = 4.54KK41 pKa = 11.7GVLLPQVALQSTTDD55 pKa = 3.33NNTISNGNVVSLLVYY70 pKa = 8.63NTNNSDD76 pKa = 3.8DD77 pKa = 4.42LKK79 pKa = 10.82AGYY82 pKa = 9.1YY83 pKa = 10.07FWDD86 pKa = 4.25GSKK89 pKa = 9.91WMSLGSQSSVSIDD102 pKa = 3.44GGTGAPDD109 pKa = 4.72DD110 pKa = 5.4DD111 pKa = 5.28NPANPQGGDD120 pKa = 2.69IYY122 pKa = 11.34VDD124 pKa = 3.43NEE126 pKa = 3.89TGDD129 pKa = 3.68IYY131 pKa = 10.6THH133 pKa = 6.85NGDD136 pKa = 3.1EE137 pKa = 4.24WVQAGSGSGSTTTPGAGSPNEE158 pKa = 4.15DD159 pKa = 3.62ANGPSDD165 pKa = 3.61PQANYY170 pKa = 10.11IPP172 pKa = 4.29

Molecular weight: 17.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D7XTH0|A0A1D7XTH0_9FLAO Iron-dependent repressor OS=Formosa sp. Hel3_A1_48 OX=1336795 GN=FORMA_12980 PE=3 SV=1
MM1 pKa = 7.84PSGKK5 pKa = 9.32KK6 pKa = 9.59RR7 pKa = 11.84KK8 pKa = 7.05RR9 pKa = 11.84HH10 pKa = 5.11KK11 pKa = 10.59VATHH15 pKa = 5.21KK16 pKa = 10.3RR17 pKa = 11.84KK18 pKa = 9.62KK19 pKa = 9.04RR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84ANRR25 pKa = 11.84HH26 pKa = 4.5KK27 pKa = 10.78KK28 pKa = 9.37KK29 pKa = 10.83

Molecular weight: 3.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1866

0

1866

625999

29

3506

335.5

37.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.628 ± 0.053

0.8 ± 0.019

5.642 ± 0.046

6.169 ± 0.046

5.326 ± 0.048

6.402 ± 0.054

1.871 ± 0.03

7.752 ± 0.049

7.462 ± 0.084

9.436 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.121 ± 0.029

6.088 ± 0.056

3.44 ± 0.038

3.661 ± 0.037

3.475 ± 0.04

6.914 ± 0.048

5.651 ± 0.064

6.243 ± 0.042

0.996 ± 0.022

3.924 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski