Sida leaf curl alphasatellite
Average proteome isoelectric point is 6.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q2WCK9|Q2WCK9_9VIRU Replication protein OS=Sida leaf curl alphasatellite OX=2169843 GN=rep PE=4 SV=1
MM1 pKa = 7.77 PALKK5 pKa = 10.33 AQWWCFTVFFLSSTAPDD22 pKa = 3.82 LVPLFEE28 pKa = 4.5 NTHH31 pKa = 4.91 VSYY34 pKa = 11.22 ACWQEE39 pKa = 4.11 EE40 pKa = 4.2 EE41 pKa = 4.78 SPTTRR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 HH49 pKa = 4.54 LQGYY53 pKa = 8.29 LQLKK57 pKa = 7.47 GQRR60 pKa = 11.84 TLNQVKK66 pKa = 10.45 AFFGDD71 pKa = 4.34 LNPHH75 pKa = 6.36 LEE77 pKa = 4.05 KK78 pKa = 10.69 QRR80 pKa = 11.84 ARR82 pKa = 11.84 KK83 pKa = 7.6 TDD85 pKa = 3.62 EE86 pKa = 4.08 ACDD89 pKa = 3.6 YY90 pKa = 11.18 CMKK93 pKa = 10.72 EE94 pKa = 4.07 EE95 pKa = 4.24 TRR97 pKa = 11.84 VSGPFEE103 pKa = 4.33 FGDD106 pKa = 3.87 YY107 pKa = 10.5 CPSGSHH113 pKa = 6.46 KK114 pKa = 10.13 RR115 pKa = 11.84 RR116 pKa = 11.84 QRR118 pKa = 11.84 EE119 pKa = 3.79 LVIRR123 pKa = 11.84 SPVRR127 pKa = 11.84 MAEE130 pKa = 4.07 EE131 pKa = 4.09 NPSVFRR137 pKa = 11.84 RR138 pKa = 11.84 VKK140 pKa = 10.51 AKK142 pKa = 10.22 LAEE145 pKa = 4.12 EE146 pKa = 4.47 EE147 pKa = 4.19 FQKK150 pKa = 9.65 TARR153 pKa = 11.84 EE154 pKa = 3.95 IQILDD159 pKa = 3.61 LKK161 pKa = 10.69 SWQLRR166 pKa = 11.84 LKK168 pKa = 9.57 TLLDD172 pKa = 4.1 RR173 pKa = 11.84 DD174 pKa = 3.84 PDD176 pKa = 3.55 DD177 pKa = 4.23 RR178 pKa = 11.84 TIFWVYY184 pKa = 10.74 GPTGGEE190 pKa = 4.17 GKK192 pKa = 8.47 STFARR197 pKa = 11.84 DD198 pKa = 3.99 LYY200 pKa = 11.15 RR201 pKa = 11.84 SGSWFYY207 pKa = 11.09 TRR209 pKa = 11.84 GGSADD214 pKa = 3.47 NVSYY218 pKa = 10.73 QYY220 pKa = 10.49 IGCLGNNIVFDD231 pKa = 3.87 IPRR234 pKa = 11.84 DD235 pKa = 3.6 KK236 pKa = 10.91 KK237 pKa = 11.07 DD238 pKa = 3.31 YY239 pKa = 10.37 LQYY242 pKa = 11.61 SLIEE246 pKa = 4.03 MFKK249 pKa = 11.15 DD250 pKa = 3.29 RR251 pKa = 11.84 LIVSNKK257 pKa = 9.63 YY258 pKa = 9.28 EE259 pKa = 4.23 PLMAPLINCIHH270 pKa = 5.9 VVVMSNFMPDD280 pKa = 3.4 FEE282 pKa = 6.58 KK283 pKa = 10.46 ISQDD287 pKa = 3.06 RR288 pKa = 11.84 VHH290 pKa = 7.04 VIPCRR295 pKa = 11.84 PCGVCLKK302 pKa = 10.5 HH303 pKa = 7.91 HH304 pKa = 6.42 MIEE307 pKa = 4.46 MKK309 pKa = 10.62 CEE311 pKa = 3.89 EE312 pKa = 4.4 YY313 pKa = 11.21 LDD315 pKa = 3.8
Molecular weight: 36.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.967
IPC2_protein 7.249
IPC_protein 7.132
Toseland 6.795
ProMoST 7.629
Dawson 7.819
Bjellqvist 8.214
Wikipedia 7.732
Rodwell 7.834
Grimsley 6.854
Solomon 7.863
Lehninger 7.892
Nozaki 8.492
DTASelect 7.966
Thurlkill 8.009
EMBOSS 8.039
Sillero 8.361
Patrickios 4.215
IPC_peptide 7.863
IPC2_peptide 7.439
IPC2.peptide.svr19 7.362
Protein with the highest isoelectric point:
>tr|Q2WCK9|Q2WCK9_9VIRU Replication protein OS=Sida leaf curl alphasatellite OX=2169843 GN=rep PE=4 SV=1
MM1 pKa = 7.77 PALKK5 pKa = 10.33 AQWWCFTVFFLSSTAPDD22 pKa = 3.82 LVPLFEE28 pKa = 4.5 NTHH31 pKa = 4.91 VSYY34 pKa = 11.22 ACWQEE39 pKa = 4.11 EE40 pKa = 4.2 EE41 pKa = 4.78 SPTTRR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 HH49 pKa = 4.54 LQGYY53 pKa = 8.29 LQLKK57 pKa = 7.47 GQRR60 pKa = 11.84 TLNQVKK66 pKa = 10.45 AFFGDD71 pKa = 4.34 LNPHH75 pKa = 6.36 LEE77 pKa = 4.05 KK78 pKa = 10.69 QRR80 pKa = 11.84 ARR82 pKa = 11.84 KK83 pKa = 7.6 TDD85 pKa = 3.62 EE86 pKa = 4.08 ACDD89 pKa = 3.6 YY90 pKa = 11.18 CMKK93 pKa = 10.72 EE94 pKa = 4.07 EE95 pKa = 4.24 TRR97 pKa = 11.84 VSGPFEE103 pKa = 4.33 FGDD106 pKa = 3.87 YY107 pKa = 10.5 CPSGSHH113 pKa = 6.46 KK114 pKa = 10.13 RR115 pKa = 11.84 RR116 pKa = 11.84 QRR118 pKa = 11.84 EE119 pKa = 3.79 LVIRR123 pKa = 11.84 SPVRR127 pKa = 11.84 MAEE130 pKa = 4.07 EE131 pKa = 4.09 NPSVFRR137 pKa = 11.84 RR138 pKa = 11.84 VKK140 pKa = 10.51 AKK142 pKa = 10.22 LAEE145 pKa = 4.12 EE146 pKa = 4.47 EE147 pKa = 4.19 FQKK150 pKa = 9.65 TARR153 pKa = 11.84 EE154 pKa = 3.95 IQILDD159 pKa = 3.61 LKK161 pKa = 10.69 SWQLRR166 pKa = 11.84 LKK168 pKa = 9.57 TLLDD172 pKa = 4.1 RR173 pKa = 11.84 DD174 pKa = 3.84 PDD176 pKa = 3.55 DD177 pKa = 4.23 RR178 pKa = 11.84 TIFWVYY184 pKa = 10.74 GPTGGEE190 pKa = 4.17 GKK192 pKa = 8.47 STFARR197 pKa = 11.84 DD198 pKa = 3.99 LYY200 pKa = 11.15 RR201 pKa = 11.84 SGSWFYY207 pKa = 11.09 TRR209 pKa = 11.84 GGSADD214 pKa = 3.47 NVSYY218 pKa = 10.73 QYY220 pKa = 10.49 IGCLGNNIVFDD231 pKa = 3.87 IPRR234 pKa = 11.84 DD235 pKa = 3.6 KK236 pKa = 10.91 KK237 pKa = 11.07 DD238 pKa = 3.31 YY239 pKa = 10.37 LQYY242 pKa = 11.61 SLIEE246 pKa = 4.03 MFKK249 pKa = 11.15 DD250 pKa = 3.29 RR251 pKa = 11.84 LIVSNKK257 pKa = 9.63 YY258 pKa = 9.28 EE259 pKa = 4.23 PLMAPLINCIHH270 pKa = 5.9 VVVMSNFMPDD280 pKa = 3.4 FEE282 pKa = 6.58 KK283 pKa = 10.46 ISQDD287 pKa = 3.06 RR288 pKa = 11.84 VHH290 pKa = 7.04 VIPCRR295 pKa = 11.84 PCGVCLKK302 pKa = 10.5 HH303 pKa = 7.91 HH304 pKa = 6.42 MIEE307 pKa = 4.46 MKK309 pKa = 10.62 CEE311 pKa = 3.89 EE312 pKa = 4.4 YY313 pKa = 11.21 LDD315 pKa = 3.8
Molecular weight: 36.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.967
IPC2_protein 7.249
IPC_protein 7.132
Toseland 6.795
ProMoST 7.629
Dawson 7.819
Bjellqvist 8.214
Wikipedia 7.732
Rodwell 7.834
Grimsley 6.854
Solomon 7.863
Lehninger 7.892
Nozaki 8.492
DTASelect 7.966
Thurlkill 8.009
EMBOSS 8.039
Sillero 8.361
Patrickios 4.215
IPC_peptide 7.863
IPC2_peptide 7.439
IPC2.peptide.svr19 7.362
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
315
315
315
315.0
36.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.444 ± 0.0
3.492 ± 0.0
6.032 ± 0.0
7.302 ± 0.0
5.397 ± 0.0
5.079 ± 0.0
2.54 ± 0.0
4.444 ± 0.0
6.349 ± 0.0
8.571 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.857 ± 0.0
3.175 ± 0.0
5.397 ± 0.0
4.444 ± 0.0
7.937 ± 0.0
6.032 ± 0.0
4.444 ± 0.0
6.032 ± 0.0
1.905 ± 0.0
4.127 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here