Acidianus tailed spindle virus
Average proteome isoelectric point is 6.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A125SJA0|A0A125SJA0_9VIRU Uncharacterized protein OS=Acidianus tailed spindle virus OX=1797140 GN=ATSV_C110 PE=4 SV=1
MM1 pKa = 8.13 DD2 pKa = 4.18 YY3 pKa = 11.31 EE4 pKa = 4.76 EE5 pKa = 4.82 VIQTIKK11 pKa = 11.01 EE12 pKa = 4.03 FFNSKK17 pKa = 9.51 NAEE20 pKa = 4.18 FNVGQPQKK28 pKa = 9.85 TEE30 pKa = 3.84 NNTISIVVDD39 pKa = 4.45 FKK41 pKa = 11.69 EE42 pKa = 4.58 PIEE45 pKa = 4.09 LTEE48 pKa = 4.04 EE49 pKa = 4.46 EE50 pKa = 4.71 YY51 pKa = 11.37 DD52 pKa = 4.18 DD53 pKa = 6.62 LYY55 pKa = 11.76 DD56 pKa = 4.56 FLDD59 pKa = 3.63 NLGFYY64 pKa = 9.85 PINGAEE70 pKa = 4.01 NQVNNYY76 pKa = 9.09 IGYY79 pKa = 8.94 KK80 pKa = 8.96 SWVGVDD86 pKa = 3.3 EE87 pKa = 4.99 FEE89 pKa = 5.34 NDD91 pKa = 3.79 DD92 pKa = 3.63 GDD94 pKa = 4.1 EE95 pKa = 4.37 VIVYY99 pKa = 6.76 YY100 pKa = 10.4 TKK102 pKa = 10.67 VCTGGKK108 pKa = 5.06 TTFFIKK114 pKa = 10.41 KK115 pKa = 9.65 IEE117 pKa = 4.04 VEE119 pKa = 4.43 LNIQEE124 pKa = 4.35 NTNEE128 pKa = 3.9 LL129 pKa = 3.64
Molecular weight: 15.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.975
IPC2_protein 3.948
IPC_protein 3.872
Toseland 3.694
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.719
Rodwell 3.706
Grimsley 3.617
Solomon 3.821
Lehninger 3.77
Nozaki 3.948
DTASelect 4.088
Thurlkill 3.732
EMBOSS 3.732
Sillero 3.986
Patrickios 2.765
IPC_peptide 3.821
IPC2_peptide 3.973
IPC2.peptide.svr19 3.891
Protein with the highest isoelectric point:
>tr|A0A125SJ73|A0A125SJ73_9VIRU Transposase OS=Acidianus tailed spindle virus OX=1797140 GN=ATSV_D194 PE=4 SV=1
MM1 pKa = 7.63 LKK3 pKa = 10.16 NLRR6 pKa = 11.84 LNNFQPQEE14 pKa = 3.94 EE15 pKa = 4.88 YY16 pKa = 11.31 VYY18 pKa = 10.04 LTYY21 pKa = 10.96 SLKK24 pKa = 10.68 NNKK27 pKa = 9.32 KK28 pKa = 9.75 EE29 pKa = 3.86 EE30 pKa = 4.19 TKK32 pKa = 10.47 ILLEE36 pKa = 4.06 NYY38 pKa = 9.45 RR39 pKa = 11.84 VLLQKK44 pKa = 10.95 ALDD47 pKa = 3.91 WLWEE51 pKa = 3.91 RR52 pKa = 11.84 TKK54 pKa = 10.44 IEE56 pKa = 3.96 RR57 pKa = 11.84 KK58 pKa = 8.19 EE59 pKa = 3.86 VKK61 pKa = 9.72 KK62 pKa = 10.82 GKK64 pKa = 10.03 KK65 pKa = 9.5 VLTKK69 pKa = 10.47 IKK71 pKa = 9.29 ITLPKK76 pKa = 9.92 KK77 pKa = 9.49 KK78 pKa = 9.66 EE79 pKa = 3.93 VYY81 pKa = 8.41 KK82 pKa = 9.68 TLRR85 pKa = 11.84 DD86 pKa = 3.61 EE87 pKa = 4.34 LEE89 pKa = 4.81 KK90 pKa = 10.68 INKK93 pKa = 8.53 LASHH97 pKa = 6.23 YY98 pKa = 9.95 VDD100 pKa = 4.19 KK101 pKa = 11.03 AISDD105 pKa = 3.9 AYY107 pKa = 10.98 SILNSWRR114 pKa = 11.84 RR115 pKa = 11.84 RR116 pKa = 11.84 AEE118 pKa = 3.76 KK119 pKa = 10.6 GQASLRR125 pKa = 11.84 KK126 pKa = 7.41 PTLRR130 pKa = 11.84 KK131 pKa = 9.31 VYY133 pKa = 10.39 VRR135 pKa = 11.84 VKK137 pKa = 8.49 STLRR141 pKa = 11.84 KK142 pKa = 9.86 VEE144 pKa = 4.12 GEE146 pKa = 4.14 SVRR149 pKa = 11.84 ITVRR153 pKa = 11.84 PYY155 pKa = 10.63 EE156 pKa = 4.1 YY157 pKa = 10.28 VTFSWSHH164 pKa = 4.22 KK165 pKa = 7.46 WFSRR169 pKa = 11.84 RR170 pKa = 11.84 VEE172 pKa = 3.99 GLEE175 pKa = 3.98 LGEE178 pKa = 4.16 PVIKK182 pKa = 9.84 EE183 pKa = 4.33 DD184 pKa = 4.34 KK185 pKa = 9.11 VHH187 pKa = 6.98 LPFRR191 pKa = 11.84 YY192 pKa = 9.32 KK193 pKa = 10.86 LPWSTPLDD201 pKa = 3.83 FLSIDD206 pKa = 3.76 SNLYY210 pKa = 9.01 TLDD213 pKa = 4.08 AYY215 pKa = 10.67 DD216 pKa = 3.83 GEE218 pKa = 4.4 KK219 pKa = 10.57 FITFSMKK226 pKa = 10.05 EE227 pKa = 4.0 LYY229 pKa = 9.96 SLKK232 pKa = 10.43 FGMEE236 pKa = 4.69 LKK238 pKa = 10.54 RR239 pKa = 11.84 SRR241 pKa = 11.84 IQSFASKK248 pKa = 9.93 HH249 pKa = 4.16 GRR251 pKa = 11.84 KK252 pKa = 9.81 GKK254 pKa = 10.36 VLMRR258 pKa = 11.84 KK259 pKa = 8.65 YY260 pKa = 10.38 SHH262 pKa = 6.9 RR263 pKa = 11.84 EE264 pKa = 3.34 RR265 pKa = 11.84 NRR267 pKa = 11.84 VLDD270 pKa = 3.91 YY271 pKa = 10.86 VHH273 pKa = 6.99 KK274 pKa = 10.32 FVNKK278 pKa = 9.9 LLEE281 pKa = 4.49 IYY283 pKa = 9.69 PVTIFAVEE291 pKa = 4.11 KK292 pKa = 10.65 LNKK295 pKa = 9.8 QSMFQDD301 pKa = 3.79 ANDD304 pKa = 3.95 KK305 pKa = 10.71 LSKK308 pKa = 10.25 KK309 pKa = 9.4 VSRR312 pKa = 11.84 TVWRR316 pKa = 11.84 TIHH319 pKa = 6.99 RR320 pKa = 11.84 VLKK323 pKa = 10.56 YY324 pKa = 9.9 KK325 pKa = 10.85 APLYY329 pKa = 10.4 GSFVKK334 pKa = 10.51 EE335 pKa = 3.95 VDD337 pKa = 3.07 PHH339 pKa = 5.85 LTSKK343 pKa = 10.44 SCPRR347 pKa = 11.84 CGWVSRR353 pKa = 11.84 KK354 pKa = 9.12 VGRR357 pKa = 11.84 TFRR360 pKa = 11.84 CEE362 pKa = 3.49 RR363 pKa = 11.84 CGFTLDD369 pKa = 3.48 RR370 pKa = 11.84 QLNASLNIYY379 pKa = 10.63 LKK381 pKa = 9.76 MCGFPHH387 pKa = 7.05 ILGIPRR393 pKa = 11.84 LWVGVIPLKK402 pKa = 10.52 GRR404 pKa = 11.84 RR405 pKa = 11.84 GNGFPRR411 pKa = 11.84 DD412 pKa = 3.65 SVEE415 pKa = 4.03 AQGLRR420 pKa = 11.84 IDD422 pKa = 3.38 IKK424 pKa = 10.96 YY425 pKa = 10.77 YY426 pKa = 10.66 EE427 pKa = 4.16 ILL429 pKa = 4.02
Molecular weight: 50.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.172
IPC2_protein 9.502
IPC_protein 9.502
Toseland 10.277
ProMoST 9.867
Dawson 10.423
Bjellqvist 10.058
Wikipedia 10.57
Rodwell 10.965
Grimsley 10.482
Solomon 10.438
Lehninger 10.409
Nozaki 10.248
DTASelect 10.058
Thurlkill 10.292
EMBOSS 10.657
Sillero 10.335
Patrickios 10.613
IPC_peptide 10.452
IPC2_peptide 8.624
IPC2.peptide.svr19 8.535
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
95
0
95
20891
45
2246
219.9
25.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.82 ± 0.249
1.063 ± 0.101
5.194 ± 0.261
6.596 ± 0.418
4.471 ± 0.284
4.562 ± 0.224
1.225 ± 0.115
9.296 ± 0.331
8.027 ± 0.537
9.913 ± 0.327
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.02 ± 0.138
6.28 ± 0.314
3.695 ± 0.301
3.049 ± 0.327
3.59 ± 0.39
7.467 ± 0.661
5.572 ± 0.475
6.989 ± 0.27
0.723 ± 0.094
5.447 ± 0.199
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here