Bergeyella cardium

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Weeksellaceae; Bergeyella

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1779 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6P1QR92|A0A6P1QR92_9FLAO DUF3853 family protein OS=Bergeyella cardium OX=1585976 GN=DBX24_01180 PE=4 SV=1
MM1 pKa = 7.81AIKK4 pKa = 9.2ITDD7 pKa = 3.4DD8 pKa = 4.47CINCGACEE16 pKa = 4.03PEE18 pKa = 4.51CPNNAIYY25 pKa = 10.44EE26 pKa = 4.37GAVDD30 pKa = 3.97WKK32 pKa = 11.15ASDD35 pKa = 3.59GTSLKK40 pKa = 10.52GAIVMPSGLSIDD52 pKa = 4.03ADD54 pKa = 3.81EE55 pKa = 4.84PQPPVSDD62 pKa = 3.49DD63 pKa = 3.83FYY65 pKa = 11.51FIVTDD70 pKa = 3.7KK71 pKa = 10.41CTEE74 pKa = 4.22CIGFHH79 pKa = 6.54EE80 pKa = 5.2EE81 pKa = 3.92PQCAAVCPVDD91 pKa = 3.96CCVPDD96 pKa = 3.64EE97 pKa = 5.24DD98 pKa = 4.64NVEE101 pKa = 4.21TEE103 pKa = 4.19EE104 pKa = 4.34QLLAKK109 pKa = 10.26KK110 pKa = 10.45AFLHH114 pKa = 6.46GEE116 pKa = 4.05EE117 pKa = 4.36

Molecular weight: 12.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6P1QXC7|A0A6P1QXC7_9FLAO 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase OS=Bergeyella cardium OX=1585976 GN=menD PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.55RR3 pKa = 11.84TFQPSEE9 pKa = 3.58RR10 pKa = 11.84KK11 pKa = 9.37KK12 pKa = 10.2RR13 pKa = 11.84NKK15 pKa = 9.71HH16 pKa = 4.09GFRR19 pKa = 11.84EE20 pKa = 4.33RR21 pKa = 11.84MSTPNGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.76GRR39 pKa = 11.84KK40 pKa = 8.45RR41 pKa = 11.84LTVSVSRR48 pKa = 11.84AKK50 pKa = 10.49RR51 pKa = 3.27

Molecular weight: 6.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1779

0

1779

588511

38

3105

330.8

37.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.495 ± 0.056

0.698 ± 0.017

5.259 ± 0.04

7.028 ± 0.066

5.154 ± 0.052

6.4 ± 0.052

1.76 ± 0.026

8.069 ± 0.057

8.311 ± 0.059

9.226 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.214 ± 0.03

5.727 ± 0.057

3.495 ± 0.036

3.6 ± 0.04

3.72 ± 0.035

6.594 ± 0.045

5.273 ± 0.044

5.787 ± 0.048

0.964 ± 0.016

4.224 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski