Encephalomyocarditis virus (strain Rueckert) (EMCV)
Average proteome isoelectric point is 7.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q66765|POLG_EMCVR Genome polyprotein OS=Encephalomyocarditis virus (strain Rueckert) OX=650129 PE=1 SV=1
MM1 pKa = 7.22 ATTMEE6 pKa = 4.38 QEE8 pKa = 4.29 TCAHH12 pKa = 6.31 SLTFEE17 pKa = 4.2 EE18 pKa = 5.27 CPKK21 pKa = 10.69 CSALQYY27 pKa = 10.97 RR28 pKa = 11.84 NGFYY32 pKa = 10.75 LLKK35 pKa = 10.25 YY36 pKa = 10.19 DD37 pKa = 4.08 EE38 pKa = 4.32 EE39 pKa = 4.81 WYY41 pKa = 10.11 PEE43 pKa = 3.9 EE44 pKa = 5.08 LLTDD48 pKa = 4.0 GEE50 pKa = 4.89 DD51 pKa = 3.84 DD52 pKa = 4.21 VFDD55 pKa = 5.35 PEE57 pKa = 5.17 LDD59 pKa = 3.5 MEE61 pKa = 5.16 VVFEE65 pKa = 4.2 LQGNSTSSDD74 pKa = 3.37 KK75 pKa = 11.45 NNSSSEE81 pKa = 3.99 GNEE84 pKa = 3.33 GVIINNFYY92 pKa = 10.97 SNQYY96 pKa = 9.32 QNSIDD101 pKa = 3.86 LSANAAGSDD110 pKa = 3.54 PPRR113 pKa = 11.84 TYY115 pKa = 11.26 GQFSNLFSGAVNAFSNMLPLLADD138 pKa = 3.41 QNTEE142 pKa = 3.69 EE143 pKa = 4.67 MEE145 pKa = 4.2 NLSDD149 pKa = 5.22 RR150 pKa = 11.84 VSQDD154 pKa = 2.87 TAGNTVTNTQSTVGRR169 pKa = 11.84 LVGYY173 pKa = 7.54 GTVHH177 pKa = 7.22 DD178 pKa = 4.95 GEE180 pKa = 5.42 HH181 pKa = 6.69 PASCADD187 pKa = 3.57 TASEE191 pKa = 4.69 KK192 pKa = 10.47 ILAVEE197 pKa = 4.51 RR198 pKa = 11.84 YY199 pKa = 7.16 YY200 pKa = 10.74 TFKK203 pKa = 11.34 VNDD206 pKa = 3.49 WTSTQKK212 pKa = 9.83 PFEE215 pKa = 4.51 YY216 pKa = 10.08 IRR218 pKa = 11.84 IPLPHH223 pKa = 6.5 VLSGEE228 pKa = 4.13 DD229 pKa = 3.11 GGVFGAALRR238 pKa = 11.84 RR239 pKa = 11.84 HH240 pKa = 5.68 YY241 pKa = 10.42 LVKK244 pKa = 10.04 TGWRR248 pKa = 11.84 VQVQCNASQFHH259 pKa = 6.7 AGGLLVFMAPEE270 pKa = 4.42 YY271 pKa = 7.56 PTLDD275 pKa = 3.24 AFAMDD280 pKa = 4.29 NRR282 pKa = 11.84 WSKK285 pKa = 11.84 DD286 pKa = 3.09 NLPNGTRR293 pKa = 11.84 TQTNKK298 pKa = 10.3 KK299 pKa = 10.1 GPFAMDD305 pKa = 3.79 HH306 pKa = 5.27 QNFWQWTLYY315 pKa = 9.56 PHH317 pKa = 6.61 QFLNLRR323 pKa = 11.84 TNTTVDD329 pKa = 3.53 LEE331 pKa = 4.47 VPYY334 pKa = 11.29 VNIAPTSSWTQHH346 pKa = 6.03 ASWTLVIAVVAPLTYY361 pKa = 9.64 STGASTSLDD370 pKa = 2.99 ITASIQPVRR379 pKa = 11.84 PVFNGLRR386 pKa = 11.84 HH387 pKa = 5.39 EE388 pKa = 4.37 TLSRR392 pKa = 11.84 QSPIPVTIRR401 pKa = 11.84 EE402 pKa = 4.35 HH403 pKa = 6.28 AGTWYY408 pKa = 8.73 STLPDD413 pKa = 3.79 STVPIYY419 pKa = 10.94 GKK421 pKa = 8.65 TPVAPSNYY429 pKa = 8.77 MVGEE433 pKa = 4.01 YY434 pKa = 10.56 KK435 pKa = 10.71 DD436 pKa = 3.81 FLEE439 pKa = 4.63 IAQIPTFIGNKK450 pKa = 8.88 IPNAVPYY457 pKa = 10.34 IEE459 pKa = 5.03 ASNTAVKK466 pKa = 8.1 TQPLATYY473 pKa = 9.91 QVTLSCSCLANTFLAALSRR492 pKa = 11.84 NFAQYY497 pKa = 10.23 RR498 pKa = 11.84 GSLVYY503 pKa = 10.12 TFVFTGTAMMKK514 pKa = 10.31 GKK516 pKa = 10.0 FLIAYY521 pKa = 6.64 TPPGAGKK528 pKa = 7.91 PTSRR532 pKa = 11.84 DD533 pKa = 2.89 QAMQATYY540 pKa = 10.43 AIWDD544 pKa = 3.77 LGLNSSYY551 pKa = 11.34 SFTVPFISPTHH562 pKa = 5.42 FRR564 pKa = 11.84 MVGTDD569 pKa = 3.13 QVNITNADD577 pKa = 2.81 GWVTVWQLTPLTYY590 pKa = 10.31 PPGCPTSAKK599 pKa = 9.81 ILTMVSAGKK608 pKa = 10.13 DD609 pKa = 3.59 FSLKK613 pKa = 10.01 MPISPAPWSPQGVEE627 pKa = 3.88 NAEE630 pKa = 4.12 KK631 pKa = 10.7 GVTEE635 pKa = 4.2 NTNATADD642 pKa = 4.11 FVAQPVYY649 pKa = 10.74 LPEE652 pKa = 4.34 NQTKK656 pKa = 9.82 VAFFYY661 pKa = 10.96 NRR663 pKa = 11.84 SSPIGAFTVKK673 pKa = 10.24 SGSLEE678 pKa = 3.98 SGFAPFSNGTCPNSVILTPGPQFDD702 pKa = 3.7 PAYY705 pKa = 10.47 DD706 pKa = 3.35 QLRR709 pKa = 11.84 PQRR712 pKa = 11.84 LTEE715 pKa = 3.26 IWGNGNEE722 pKa = 4.24 EE723 pKa = 4.11 TSKK726 pKa = 10.78 VFPLKK731 pKa = 10.66 SKK733 pKa = 10.65 QDD735 pKa = 3.37 YY736 pKa = 10.16 SFCLFSPFVYY746 pKa = 9.86 YY747 pKa = 10.62 KK748 pKa = 10.65 CDD750 pKa = 3.74 LEE752 pKa = 4.77 VTLSPHH758 pKa = 5.53 TSGNHH763 pKa = 4.73 GLLVRR768 pKa = 11.84 WCPTGTPTKK777 pKa = 9.17 PTTQVLHH784 pKa = 6.0 EE785 pKa = 4.23 VSSLSEE791 pKa = 3.65 GRR793 pKa = 11.84 TPQVYY798 pKa = 10.02 SAGPGISNQISFVVPYY814 pKa = 9.93 NSPLSVLSAVWYY826 pKa = 9.6 NGHH829 pKa = 6.67 KK830 pKa = 10.62 RR831 pKa = 11.84 FDD833 pKa = 3.7 NTGSLGIAPNSDD845 pKa = 3.07 FGTLFFAGTKK855 pKa = 9.62 PDD857 pKa = 3.11 IKK859 pKa = 9.71 FTVYY863 pKa = 10.44 LRR865 pKa = 11.84 YY866 pKa = 9.18 KK867 pKa = 9.47 NKK869 pKa = 10.13 RR870 pKa = 11.84 VFCPRR875 pKa = 11.84 PTVFFPWPTSGDD887 pKa = 4.06 KK888 pKa = 10.17 IDD890 pKa = 3.52 MTPRR894 pKa = 11.84 AGVLMLEE901 pKa = 4.66 SPNALDD907 pKa = 4.17 ISRR910 pKa = 11.84 TYY912 pKa = 8.59 PTLHH916 pKa = 6.76 VLIQFNHH923 pKa = 6.71 RR924 pKa = 11.84 GLEE927 pKa = 3.93 VRR929 pKa = 11.84 LFRR932 pKa = 11.84 HH933 pKa = 4.48 GHH935 pKa = 5.11 FWAEE939 pKa = 3.74 TRR941 pKa = 11.84 ADD943 pKa = 3.58 VILRR947 pKa = 11.84 SKK949 pKa = 8.94 TKK951 pKa = 10.16 QVSFLSNGNYY961 pKa = 9.98 PSMDD965 pKa = 2.94 SRR967 pKa = 11.84 APWNPWKK974 pKa = 9.36 NTYY977 pKa = 9.41 QAVLRR982 pKa = 11.84 AEE984 pKa = 4.37 PCRR987 pKa = 11.84 VTMDD991 pKa = 3.2 IYY993 pKa = 10.69 YY994 pKa = 10.53 KK995 pKa = 10.13 RR996 pKa = 11.84 VRR998 pKa = 11.84 PFRR1001 pKa = 11.84 LPLVQKK1007 pKa = 7.97 EE1008 pKa = 4.19 WPVRR1012 pKa = 11.84 EE1013 pKa = 3.98 EE1014 pKa = 4.17 NVFGLYY1020 pKa = 10.21 RR1021 pKa = 11.84 IFNAHH1026 pKa = 5.23 YY1027 pKa = 9.74 AGYY1030 pKa = 9.32 FADD1033 pKa = 5.5 LLIHH1037 pKa = 7.49 DD1038 pKa = 5.04 IEE1040 pKa = 4.65 TNPGPFMFRR1049 pKa = 11.84 PRR1051 pKa = 11.84 KK1052 pKa = 9.48 QVFQTQGAAVSSMAQTLLPNDD1073 pKa = 4.22 LASKK1077 pKa = 10.94 AMGSAFTALLDD1088 pKa = 4.59 ANEE1091 pKa = 4.64 DD1092 pKa = 3.35 AQKK1095 pKa = 11.16 AMKK1098 pKa = 9.93 IIKK1101 pKa = 8.32 TLSSLSDD1108 pKa = 2.55 AWEE1111 pKa = 4.16 NVKK1114 pKa = 8.62 EE1115 pKa = 4.18 TLNNPEE1121 pKa = 3.95 FWKK1124 pKa = 10.67 QLLSRR1129 pKa = 11.84 CVQLIAGMTIAVMHH1143 pKa = 7.13 PDD1145 pKa = 4.23 PLTLLCLGTLTAAEE1159 pKa = 4.29 ITSQTSLCEE1168 pKa = 3.77 EE1169 pKa = 3.88 IAAKK1173 pKa = 10.3 FKK1175 pKa = 10.58 TIFITPPPRR1184 pKa = 11.84 FPTISLFQQQSPLKK1198 pKa = 9.2 QVNDD1202 pKa = 3.3 IFSLAKK1208 pKa = 10.44 NLDD1211 pKa = 3.55 WAVKK1215 pKa = 7.96 TVEE1218 pKa = 5.14 KK1219 pKa = 10.73 VVDD1222 pKa = 3.85 WFGTWIVQEE1231 pKa = 4.51 EE1232 pKa = 4.55 KK1233 pKa = 10.85 EE1234 pKa = 4.11 QTLDD1238 pKa = 3.28 QLLQRR1243 pKa = 11.84 FPEE1246 pKa = 4.15 HH1247 pKa = 6.89 AKK1249 pKa = 10.44 RR1250 pKa = 11.84 ISDD1253 pKa = 3.95 LRR1255 pKa = 11.84 NGMAAYY1261 pKa = 9.77 VEE1263 pKa = 4.64 CKK1265 pKa = 10.0 EE1266 pKa = 4.39 SFDD1269 pKa = 4.7 FFEE1272 pKa = 4.53 KK1273 pKa = 10.57 LYY1275 pKa = 10.54 NQAVKK1280 pKa = 10.52 EE1281 pKa = 4.04 KK1282 pKa = 8.69 RR1283 pKa = 11.84 TGIAAVCEE1291 pKa = 4.35 KK1292 pKa = 10.19 FRR1294 pKa = 11.84 QKK1296 pKa = 10.39 HH1297 pKa = 4.3 DD1298 pKa = 3.32 HH1299 pKa = 5.64 ATARR1303 pKa = 11.84 CEE1305 pKa = 3.67 PVVIVLRR1312 pKa = 11.84 GDD1314 pKa = 3.57 AGQGKK1319 pKa = 9.02 SLSSQVIAQAVSKK1332 pKa = 9.34 TIFGRR1337 pKa = 11.84 QSVYY1341 pKa = 11.12 SLPPDD1346 pKa = 3.27 SDD1348 pKa = 3.95 FFDD1351 pKa = 5.15 GYY1353 pKa = 10.57 EE1354 pKa = 4.0 NQFAAIMDD1362 pKa = 4.39 DD1363 pKa = 4.34 LGQNPDD1369 pKa = 3.25 GSDD1372 pKa = 3.21 FTTFCQMVSTTNFLPNMASLEE1393 pKa = 4.25 RR1394 pKa = 11.84 KK1395 pKa = 7.2 GTPFTSQLVVATTNLPEE1412 pKa = 4.07 FRR1414 pKa = 11.84 PVTIAHH1420 pKa = 5.72 YY1421 pKa = 8.95 PAVEE1425 pKa = 3.72 RR1426 pKa = 11.84 RR1427 pKa = 11.84 ITFDD1431 pKa = 3.31 YY1432 pKa = 10.6 SVSAGPVCSKK1442 pKa = 10.03 TEE1444 pKa = 3.73 AGYY1447 pKa = 10.73 KK1448 pKa = 9.84 VLDD1451 pKa = 3.47 VEE1453 pKa = 4.75 RR1454 pKa = 11.84 AFRR1457 pKa = 11.84 PTGEE1461 pKa = 4.22 APLPCFQNNCLFLEE1475 pKa = 4.44 KK1476 pKa = 10.57 AGLQFRR1482 pKa = 11.84 DD1483 pKa = 3.25 NRR1485 pKa = 11.84 TKK1487 pKa = 10.67 EE1488 pKa = 4.02 IISLVDD1494 pKa = 3.75 VIEE1497 pKa = 4.3 RR1498 pKa = 11.84 AVARR1502 pKa = 11.84 IEE1504 pKa = 4.1 RR1505 pKa = 11.84 KK1506 pKa = 9.98 KK1507 pKa = 10.88 KK1508 pKa = 10.58 VLTTVQTLVAQGPVDD1523 pKa = 3.57 EE1524 pKa = 5.04 VSFHH1528 pKa = 7.41 SVVQQLKK1535 pKa = 10.26 ARR1537 pKa = 11.84 QQATDD1542 pKa = 3.4 EE1543 pKa = 4.08 QLEE1546 pKa = 4.18 EE1547 pKa = 4.06 LQEE1550 pKa = 4.22 AFAKK1554 pKa = 9.31 VQEE1557 pKa = 4.35 RR1558 pKa = 11.84 NSVFSDD1564 pKa = 3.08 WLKK1567 pKa = 10.75 ISAMLCAATLALSQVVKK1584 pKa = 9.56 MAKK1587 pKa = 9.61 AVKK1590 pKa = 10.33 QMVKK1594 pKa = 9.8 PDD1596 pKa = 3.76 LVRR1599 pKa = 11.84 VQLDD1603 pKa = 3.6 EE1604 pKa = 4.58 QEE1606 pKa = 3.97 QGPYY1610 pKa = 10.66 NEE1612 pKa = 4.38 TARR1615 pKa = 11.84 VKK1617 pKa = 10.61 PKK1619 pKa = 9.67 TLQLLDD1625 pKa = 3.5 IQGPNPVMDD1634 pKa = 4.12 FEE1636 pKa = 5.24 KK1637 pKa = 11.0 YY1638 pKa = 7.99 VAKK1641 pKa = 10.49 HH1642 pKa = 3.65 VTAPIGFVYY1651 pKa = 8.39 PTGVSTQTCLLVRR1664 pKa = 11.84 GRR1666 pKa = 11.84 TLVVNRR1672 pKa = 11.84 HH1673 pKa = 4.85 MAEE1676 pKa = 4.1 SDD1678 pKa = 2.92 WTSIVVRR1685 pKa = 11.84 GVTHH1689 pKa = 6.71 ARR1691 pKa = 11.84 STVKK1695 pKa = 10.22 ILAIAKK1701 pKa = 9.59 AGKK1704 pKa = 8.22 EE1705 pKa = 3.99 TDD1707 pKa = 3.22 VSFIRR1712 pKa = 11.84 LSSGPLFRR1720 pKa = 11.84 DD1721 pKa = 3.32 NTSKK1725 pKa = 10.38 FVKK1728 pKa = 10.47 AGDD1731 pKa = 3.78 VLPTGAAPVTGIMNTDD1747 pKa = 3.08 IPMMYY1752 pKa = 9.79 TGTFLKK1758 pKa = 10.58 AGVSVPVEE1766 pKa = 3.77 TGQTFNHH1773 pKa = 6.9 CIHH1776 pKa = 6.42 YY1777 pKa = 9.89 KK1778 pKa = 10.27 ANTRR1782 pKa = 11.84 KK1783 pKa = 9.08 GWCGSALLADD1793 pKa = 4.68 LGGSKK1798 pKa = 10.23 KK1799 pKa = 10.29 ILGIHH1804 pKa = 6.07 SAGSMGIAAASIVSQEE1820 pKa = 4.16 MIRR1823 pKa = 11.84 AVVNAFEE1830 pKa = 4.22 PQGALEE1836 pKa = 4.5 RR1837 pKa = 11.84 LPDD1840 pKa = 3.81 GPRR1843 pKa = 11.84 IHH1845 pKa = 6.73 VPRR1848 pKa = 11.84 KK1849 pKa = 6.56 TALRR1853 pKa = 11.84 PTVARR1858 pKa = 11.84 QVFQPAYY1865 pKa = 10.03 APAVLSKK1872 pKa = 10.45 FDD1874 pKa = 3.98 PRR1876 pKa = 11.84 TEE1878 pKa = 4.02 ADD1880 pKa = 3.08 VDD1882 pKa = 3.78 EE1883 pKa = 4.76 VAFSKK1888 pKa = 9.05 HH1889 pKa = 4.77 TSNQEE1894 pKa = 3.55 SLPPVFRR1901 pKa = 11.84 MVAKK1905 pKa = 10.1 EE1906 pKa = 3.47 YY1907 pKa = 10.69 ANRR1910 pKa = 11.84 VFTLLGKK1917 pKa = 10.34 DD1918 pKa = 3.26 NGRR1921 pKa = 11.84 LTVKK1925 pKa = 10.26 QALEE1929 pKa = 4.13 GLEE1932 pKa = 4.85 GMDD1935 pKa = 3.67 PMDD1938 pKa = 4.99 RR1939 pKa = 11.84 NTSPGLPYY1947 pKa = 9.57 TALGMRR1953 pKa = 11.84 RR1954 pKa = 11.84 TDD1956 pKa = 3.44 VVDD1959 pKa = 3.34 WEE1961 pKa = 4.62 SATLIPFAAEE1971 pKa = 3.96 RR1972 pKa = 11.84 LRR1974 pKa = 11.84 KK1975 pKa = 9.16 MNEE1978 pKa = 3.54 GDD1980 pKa = 3.42 FSEE1983 pKa = 5.29 VVYY1986 pKa = 8.32 QTFLKK1991 pKa = 10.94 DD1992 pKa = 3.23 EE1993 pKa = 4.37 LRR1995 pKa = 11.84 PIEE1998 pKa = 4.28 KK1999 pKa = 10.13 VQAAKK2004 pKa = 9.81 TRR2006 pKa = 11.84 IVDD2009 pKa = 3.44 VPPFEE2014 pKa = 4.81 HH2015 pKa = 7.2 CILGRR2020 pKa = 11.84 QLLGKK2025 pKa = 9.66 FASKK2029 pKa = 10.18 FQTQPGLEE2037 pKa = 4.17 LGSAIGCDD2045 pKa = 3.77 PDD2047 pKa = 3.88 VHH2049 pKa = 5.4 WTAFGVAMQGFEE2061 pKa = 3.73 RR2062 pKa = 11.84 VYY2064 pKa = 11.13 DD2065 pKa = 3.54 VDD2067 pKa = 3.68 YY2068 pKa = 11.66 SNFDD2072 pKa = 3.62 STHH2075 pKa = 5.08 SVAMFRR2081 pKa = 11.84 LLAEE2085 pKa = 4.24 EE2086 pKa = 5.15 FFTPEE2091 pKa = 3.88 NGFDD2095 pKa = 3.87 PLTRR2099 pKa = 11.84 EE2100 pKa = 4.13 YY2101 pKa = 11.08 LEE2103 pKa = 4.32 SLAISTHH2110 pKa = 4.99 AFEE2113 pKa = 5.09 EE2114 pKa = 4.33 KK2115 pKa = 10.31 RR2116 pKa = 11.84 FLITGGLPSGCAATSMLNTIMNNIIIRR2143 pKa = 11.84 AGLYY2147 pKa = 8.18 LTYY2150 pKa = 11.0 KK2151 pKa = 10.43 NFEE2154 pKa = 4.0 FDD2156 pKa = 3.59 DD2157 pKa = 4.38 VKK2159 pKa = 11.23 VLSYY2163 pKa = 11.64 GDD2165 pKa = 4.22 DD2166 pKa = 3.53 LLVATNYY2173 pKa = 10.27 QLDD2176 pKa = 3.79 FDD2178 pKa = 4.43 KK2179 pKa = 11.36 VRR2181 pKa = 11.84 ASLAKK2186 pKa = 8.98 TGYY2189 pKa = 10.39 KK2190 pKa = 8.42 ITPANTTSTFPLNSTLEE2207 pKa = 4.04 DD2208 pKa = 3.53 VVFLKK2213 pKa = 10.71 RR2214 pKa = 11.84 KK2215 pKa = 8.72 FKK2217 pKa = 11.11 KK2218 pKa = 9.85 EE2219 pKa = 3.66 GPLYY2223 pKa = 9.8 RR2224 pKa = 11.84 PVMNRR2229 pKa = 11.84 EE2230 pKa = 3.82 ALEE2233 pKa = 4.29 AMLSYY2238 pKa = 10.34 YY2239 pKa = 10.54 RR2240 pKa = 11.84 PGTLSEE2246 pKa = 4.39 KK2247 pKa = 9.62 LTSITMLAVHH2257 pKa = 6.63 SGKK2260 pKa = 9.75 QEE2262 pKa = 3.85 YY2263 pKa = 10.34 DD2264 pKa = 2.95 RR2265 pKa = 11.84 LFAPFRR2271 pKa = 11.84 EE2272 pKa = 4.46 VGVVVPSFEE2281 pKa = 4.15 SVEE2284 pKa = 3.97 YY2285 pKa = 10.07 RR2286 pKa = 11.84 WRR2288 pKa = 11.84 SLFWW2292 pKa = 5.37
Molecular weight: 255.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.218
IPC2_protein 6.275
IPC_protein 6.326
Toseland 6.532
ProMoST 6.737
Dawson 6.678
Bjellqvist 6.693
Wikipedia 6.693
Rodwell 6.678
Grimsley 6.649
Solomon 6.678
Lehninger 6.678
Nozaki 6.985
DTASelect 7.102
Thurlkill 7.132
EMBOSS 7.102
Sillero 7.073
Patrickios 4.228
IPC_peptide 6.693
IPC2_peptide 6.825
IPC2.peptide.svr19 6.818
Protein with the highest isoelectric point:
>sp|Q66765|POLG_EMCVR Genome polyprotein OS=Encephalomyocarditis virus (strain Rueckert) OX=650129 PE=1 SV=1
PP1 pKa = 7.58 FMFRR5 pKa = 11.84 PRR7 pKa = 11.84 KK8 pKa = 9.34 QVFPDD13 pKa = 3.59 PRR15 pKa = 11.84 SGSVINGSNPTAEE28 pKa = 4.5 RR29 pKa = 11.84 PCQQSYY35 pKa = 10.92 GISFYY40 pKa = 11.44 GFARR44 pKa = 11.84 CQRR47 pKa = 11.84 GRR49 pKa = 11.84 PKK51 pKa = 10.93 SNEE54 pKa = 3.79 DD55 pKa = 3.16 YY56 pKa = 11.29 KK57 pKa = 11.16 DD58 pKa = 3.06 IKK60 pKa = 10.83 FSIGCMGKK68 pKa = 9.79 CKK70 pKa = 10.43 RR71 pKa = 11.84 NTKK74 pKa = 8.0 QPRR77 pKa = 11.84 VLEE80 pKa = 4.21 AALEE84 pKa = 4.11 QMCAADD90 pKa = 4.6 CRR92 pKa = 11.84 DD93 pKa = 4.55 DD94 pKa = 3.92 NSSDD98 pKa = 3.12 ASGPFDD104 pKa = 3.95 SALLRR109 pKa = 11.84 NIDD112 pKa = 3.02 GRR114 pKa = 11.84 RR115 pKa = 11.84 DD116 pKa = 3.61 YY117 pKa = 11.4 KK118 pKa = 10.36 PDD120 pKa = 3.06 KK121 pKa = 10.33 SVRR124 pKa = 11.84 RR125 pKa = 11.84 NSSS128 pKa = 2.59
Molecular weight: 14.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.984
IPC2_protein 8.756
IPC_protein 8.756
Toseland 9.37
ProMoST 9.209
Dawson 9.663
Bjellqvist 9.545
Wikipedia 9.809
Rodwell 9.897
Grimsley 9.677
Solomon 9.75
Lehninger 9.721
Nozaki 9.765
DTASelect 9.414
Thurlkill 9.56
EMBOSS 9.838
Sillero 9.721
Patrickios 5.372
IPC_peptide 9.75
IPC2_peptide 8.595
IPC2.peptide.svr19 7.795
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
2420
128
2292
1210.0
134.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.851 ± 0.741
1.529 ± 1.461
4.917 ± 1.7
5.331 ± 1.02
5.579 ± 0.051
5.909 ± 0.519
1.694 ± 0.784
3.967 ± 0.028
5.124 ± 0.882
8.182 ± 2.339
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.231 ± 0.052
4.545 ± 0.427
6.446 ± 0.632
4.463 ± 0.104
5.207 ± 3.012
7.231 ± 1.714
7.603 ± 2.794
7.521 ± 2.033
1.364 ± 0.631
3.306 ± 0.084
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here