Halovirus HSTV-1
Average proteome isoelectric point is 4.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R9QTN4|R9QTN4_9VIRU PCNA protein OS=Halovirus HSTV-1 OX=1262530 GN=40 PE=3 SV=1
MM1 pKa = 7.64 TDD3 pKa = 3.15 GAVDD7 pKa = 3.48 ADD9 pKa = 4.13 YY10 pKa = 11.78 GEE12 pKa = 4.77 GLQDD16 pKa = 3.45 YY17 pKa = 9.48 YY18 pKa = 11.8 DD19 pKa = 3.88 RR20 pKa = 11.84 VADD23 pKa = 4.03 ALEE26 pKa = 4.55 DD27 pKa = 3.84 VPGEE31 pKa = 4.05 PVVGGCAIDD40 pKa = 3.11 IVTRR44 pKa = 11.84 QVVYY48 pKa = 10.86 VVDD51 pKa = 5.05 RR52 pKa = 11.84 VADD55 pKa = 3.83 SCVEE59 pKa = 4.04 YY60 pKa = 11.23 YY61 pKa = 10.58 DD62 pKa = 5.47 AEE64 pKa = 4.47 GFDD67 pKa = 3.56 LVTYY71 pKa = 10.44 KK72 pKa = 9.01 MHH74 pKa = 7.22 PFLPGISVEE83 pKa = 4.19 NAVYY87 pKa = 10.27 EE88 pKa = 4.34 CVYY91 pKa = 11.15 VDD93 pKa = 4.42 GNPQNTHH100 pKa = 5.66 KK101 pKa = 10.68 PGRR104 pKa = 11.84 TYY106 pKa = 11.11 DD107 pKa = 3.5 FPTARR112 pKa = 11.84 LMPFPVGIATDD123 pKa = 3.71 TYY125 pKa = 11.04 EE126 pKa = 4.08 VGGVV130 pKa = 3.17
Molecular weight: 14.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.855
IPC2_protein 3.872
IPC_protein 3.846
Toseland 3.63
ProMoST 4.024
Dawson 3.846
Bjellqvist 4.012
Wikipedia 3.795
Rodwell 3.681
Grimsley 3.541
Solomon 3.834
Lehninger 3.795
Nozaki 3.973
DTASelect 4.215
Thurlkill 3.694
EMBOSS 3.808
Sillero 3.973
Patrickios 0.223
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.876
Protein with the highest isoelectric point:
>tr|R9QTM6|R9QTM6_9VIRU Uncharacterized protein OS=Halovirus HSTV-1 OX=1262530 GN=5 PE=4 SV=1
MM1 pKa = 7.53 AVGRR5 pKa = 11.84 SHH7 pKa = 6.85 TPHH10 pKa = 6.5 TAGSAVDD17 pKa = 3.45 AAGDD21 pKa = 3.43 VSGNVSRR28 pKa = 11.84 TAPRR32 pKa = 11.84 EE33 pKa = 4.01 DD34 pKa = 3.63 RR35 pKa = 11.84 RR36 pKa = 11.84 GTAPNRR42 pKa = 11.84 QTYY45 pKa = 9.97 AIWPTMKK52 pKa = 9.41 TCRR55 pKa = 11.84 ATWTRR60 pKa = 11.84 HH61 pKa = 4.67 SLSPTIGLRR70 pKa = 11.84 SHH72 pKa = 6.49 HH73 pKa = 5.86 TTPILEE79 pKa = 4.3 RR80 pKa = 11.84 VGHH83 pKa = 5.98 SSGFDD88 pKa = 3.21 SLSLRR93 pKa = 11.84 RR94 pKa = 11.84 RR95 pKa = 11.84 LRR97 pKa = 11.84 KK98 pKa = 9.79 GVWIRR103 pKa = 11.84 SWARR107 pKa = 11.84 FFRR110 pKa = 11.84 VVSPRR115 pKa = 11.84 PYY117 pKa = 8.06 NTRR120 pKa = 11.84 VKK122 pKa = 9.8 TRR124 pKa = 11.84 AEE126 pKa = 3.84 RR127 pKa = 11.84 RR128 pKa = 11.84 RR129 pKa = 11.84 HH130 pKa = 4.89 FSHH133 pKa = 6.98 TFRR136 pKa = 11.84 LAYY139 pKa = 9.09 MNHH142 pKa = 7.15 PALCSISHH150 pKa = 6.85 DD151 pKa = 3.64 CVVDD155 pKa = 4.24 VMATGQSRR163 pKa = 11.84 TTDD166 pKa = 3.28 KK167 pKa = 10.98 PSSTDD172 pKa = 3.13 YY173 pKa = 10.73 RR174 pKa = 11.84 NCLITPMSSDD184 pKa = 3.48 PPLIRR189 pKa = 11.84 PEE191 pKa = 4.07 HH192 pKa = 6.6 VIDD195 pKa = 4.16 ALLVGLLAFFGVILADD211 pKa = 3.48 VLTALLQGRR220 pKa = 11.84 VVYY223 pKa = 8.47 LTVSDD228 pKa = 3.73 IAARR232 pKa = 11.84 TPTGVVAFGLTFVFQWARR250 pKa = 11.84 ARR252 pKa = 11.84 GIDD255 pKa = 3.39 VLAAYY260 pKa = 9.96 RR261 pKa = 11.84 KK262 pKa = 10.03 FKK264 pKa = 10.94 DD265 pKa = 3.4 SLPP268 pKa = 3.84
Molecular weight: 29.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.24
IPC2_protein 9.882
IPC_protein 11.125
Toseland 11.169
ProMoST 11.55
Dawson 11.199
Bjellqvist 11.14
Wikipedia 11.623
Rodwell 11.023
Grimsley 11.257
Solomon 11.608
Lehninger 11.52
Nozaki 11.169
DTASelect 11.14
Thurlkill 11.169
EMBOSS 11.652
Sillero 11.184
Patrickios 10.73
IPC_peptide 11.608
IPC2_peptide 10.584
IPC2.peptide.svr19 9.19
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
53
0
53
10139
28
881
191.3
21.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.459 ± 0.322
0.937 ± 0.131
9.814 ± 0.285
8.334 ± 0.507
2.673 ± 0.181
7.792 ± 0.338
1.825 ± 0.18
4.458 ± 0.358
2.821 ± 0.198
6.687 ± 0.328
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.121 ± 0.15
3.639 ± 0.34
4.379 ± 0.209
3.61 ± 0.189
6.076 ± 0.295
6.283 ± 0.323
7.299 ± 0.396
7.427 ± 0.285
1.696 ± 0.14
2.673 ± 0.26
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here