Clostridium sp. CAG:452

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1317 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6YGH3|R6YGH3_9CLOT Ferredoxin OS=Clostridium sp. CAG:452 OX=1262810 GN=BN664_00978 PE=4 SV=1
MM1 pKa = 7.08EE2 pKa = 4.13QVEE5 pKa = 4.74NPMVMPEE12 pKa = 3.9YY13 pKa = 9.99EE14 pKa = 4.35YY15 pKa = 11.31KK16 pKa = 10.26SDD18 pKa = 4.25YY19 pKa = 11.08IPDD22 pKa = 3.78NVWAEE27 pKa = 4.07RR28 pKa = 11.84EE29 pKa = 4.01DD30 pKa = 3.98RR31 pKa = 11.84DD32 pKa = 3.88YY33 pKa = 11.55EE34 pKa = 4.02DD35 pKa = 5.21SIFEE39 pKa = 4.17EE40 pKa = 4.75EE41 pKa = 4.18LL42 pKa = 3.44

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6Y8J6|R6Y8J6_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:452 OX=1262810 GN=BN664_00935 PE=4 SV=1
MM1 pKa = 7.46ARR3 pKa = 11.84IIVFNNDD10 pKa = 2.93TNRR13 pKa = 11.84MEE15 pKa = 4.01NYY17 pKa = 9.46YY18 pKa = 10.64RR19 pKa = 11.84NEE21 pKa = 4.0NEE23 pKa = 3.83AMPYY27 pKa = 8.87NANRR31 pKa = 11.84SLTVGEE37 pKa = 4.38FRR39 pKa = 11.84GSSKK43 pKa = 11.06SPTLWTTKK51 pKa = 10.23RR52 pKa = 11.84AMQSFNTTRR61 pKa = 11.84YY62 pKa = 10.19LFGAPIPVGFAFKK75 pKa = 10.56RR76 pKa = 11.84PWEE79 pKa = 4.04RR80 pKa = 11.84RR81 pKa = 11.84TWFAKK86 pKa = 8.69STLRR90 pKa = 11.84AEE92 pKa = 4.29FHH94 pKa = 5.8LTQVKK99 pKa = 10.02CC100 pKa = 3.67

Molecular weight: 11.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1317

0

1317

361571

29

2487

274.5

31.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.478 ± 0.059

1.13 ± 0.025

5.319 ± 0.056

8.484 ± 0.079

3.854 ± 0.046

5.841 ± 0.06

1.157 ± 0.024

10.233 ± 0.097

10.238 ± 0.084

8.404 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.36 ± 0.028

6.719 ± 0.075

2.7 ± 0.035

2.671 ± 0.038

3.31 ± 0.048

5.568 ± 0.052

5.548 ± 0.06

6.085 ± 0.056

0.615 ± 0.018

4.286 ± 0.057
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski