Propionibacterium phage PHL092M00
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0E3DKZ3|A0A0E3DKZ3_9CAUD Uncharacterized protein OS=Propionibacterium phage PHL092M00 OX=1500813 GN=PHL092M00_27 PE=4 SV=1
MM1 pKa = 7.48 NNTHH5 pKa = 7.12 NIPSTALKK13 pKa = 9.41 TVVHH17 pKa = 7.13 RR18 pKa = 11.84 IIQQQPTNMQQLEE31 pKa = 4.44 NIVDD35 pKa = 3.96 SVEE38 pKa = 3.74 NQYY41 pKa = 10.59 RR42 pKa = 11.84 VPISLDD48 pKa = 3.34 NVNLTVKK55 pKa = 10.14 EE56 pKa = 4.09 VSLDD60 pKa = 3.69 DD61 pKa = 4.6 LAIDD65 pKa = 4.03 QDD67 pKa = 4.19 TLDD70 pKa = 4.0 EE71 pKa = 4.51 CSEE74 pKa = 4.21 ILWDD78 pKa = 4.43 CDD80 pKa = 4.08 SAGHH84 pKa = 6.03 PTNNSNTRR92 pKa = 11.84 GMPDD96 pKa = 3.29 DD97 pKa = 3.91 TQASQEE103 pKa = 4.31 AIDD106 pKa = 3.89 WLAGIAYY113 pKa = 8.03 QAKK116 pKa = 9.97 LLQAEE121 pKa = 5.02 ADD123 pKa = 4.71 DD124 pKa = 4.21 IMQSIISHH132 pKa = 6.84 RR133 pKa = 11.84 DD134 pKa = 2.74 NHH136 pKa = 6.49 KK137 pKa = 10.87 NVIGQDD143 pKa = 3.51 VLHH146 pKa = 6.12 QANDD150 pKa = 3.6 IISACLYY157 pKa = 9.84 LDD159 pKa = 3.84 QMLEE163 pKa = 4.04 DD164 pKa = 4.1 TLDD167 pKa = 3.95 DD168 pKa = 3.93 NLL170 pKa = 4.84
Molecular weight: 19.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.076
IPC2_protein 3.973
IPC_protein 3.973
Toseland 3.757
ProMoST 4.151
Dawson 3.986
Bjellqvist 4.139
Wikipedia 3.935
Rodwell 3.808
Grimsley 3.668
Solomon 3.973
Lehninger 3.935
Nozaki 4.101
DTASelect 4.368
Thurlkill 3.821
EMBOSS 3.948
Sillero 4.101
Patrickios 3.249
IPC_peptide 3.973
IPC2_peptide 4.075
IPC2.peptide.svr19 4.011
Protein with the highest isoelectric point:
>tr|A0A0E3DMQ3|A0A0E3DMQ3_9CAUD Tape measure protein OS=Propionibacterium phage PHL092M00 OX=1500813 GN=PHL092M00_14 PE=4 SV=1
MM1 pKa = 7.13 AQDD4 pKa = 3.63 VNVKK8 pKa = 10.51 LNLPGIRR15 pKa = 11.84 EE16 pKa = 4.17 VLKK19 pKa = 11.04 SSGVQSMLAEE29 pKa = 4.26 RR30 pKa = 11.84 GEE32 pKa = 4.3 RR33 pKa = 11.84 VRR35 pKa = 11.84 RR36 pKa = 11.84 AAAANVGGNAFDD48 pKa = 3.75 RR49 pKa = 11.84 AQYY52 pKa = 10.62 RR53 pKa = 11.84 SGLSSEE59 pKa = 4.53 VQVHH63 pKa = 4.93 RR64 pKa = 11.84 VEE66 pKa = 3.97 AVARR70 pKa = 11.84 IGTTYY75 pKa = 10.82 KK76 pKa = 9.95 GGKK79 pKa = 9.29 RR80 pKa = 11.84 IEE82 pKa = 4.49 AKK84 pKa = 10.34 HH85 pKa = 5.05 GTLARR90 pKa = 11.84 SIGAASS96 pKa = 3.15
Molecular weight: 10.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.315
IPC2_protein 9.809
IPC_protein 10.877
Toseland 11.038
ProMoST 11.052
Dawson 11.096
Bjellqvist 10.891
Wikipedia 11.389
Rodwell 11.169
Grimsley 11.125
Solomon 11.359
Lehninger 11.301
Nozaki 11.023
DTASelect 10.891
Thurlkill 11.038
EMBOSS 11.477
Sillero 11.038
Patrickios 10.935
IPC_peptide 11.359
IPC2_peptide 9.999
IPC2.peptide.svr19 8.699
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
45
0
45
8610
43
921
191.3
20.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.965 ± 0.74
1.208 ± 0.181
6.655 ± 0.484
4.762 ± 0.427
2.776 ± 0.22
8.734 ± 0.667
2.706 ± 0.41
4.762 ± 0.364
4.019 ± 0.334
7.456 ± 0.283
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.648 ± 0.314
3.67 ± 0.288
5.029 ± 0.343
4.309 ± 0.377
6.26 ± 0.427
6.481 ± 0.325
6.074 ± 0.582
7.956 ± 0.84
1.94 ± 0.214
2.59 ± 0.299
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here