Cellulomonas sp. B6

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Cellulomonadaceae; Cellulomonas; unclassified Cellulomonas

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3428 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0V8SE95|A0A0V8SE95_9CELL Chitinase OS=Cellulomonas sp. B6 OX=1295626 GN=ATM99_17175 PE=3 SV=1
MM1 pKa = 6.99TPSRR5 pKa = 11.84RR6 pKa = 11.84AAAPAAAATLLLALAACTSTSDD28 pKa = 3.45AAEE31 pKa = 4.56APDD34 pKa = 4.3SQWSDD39 pKa = 3.29LADD42 pKa = 3.56SAACADD48 pKa = 4.16LRR50 pKa = 11.84EE51 pKa = 4.24AHH53 pKa = 7.2PDD55 pKa = 3.41LVGTTQTNAINPHH68 pKa = 4.85TPGYY72 pKa = 10.43EE73 pKa = 4.11VIDD76 pKa = 4.15PEE78 pKa = 4.48NPDD81 pKa = 3.07RR82 pKa = 11.84FMGFDD87 pKa = 3.39IDD89 pKa = 3.76LGEE92 pKa = 5.45AIGACLGFDD101 pKa = 3.37VDD103 pKa = 4.14YY104 pKa = 11.11VAVGFSEE111 pKa = 5.75LIPTVASGQADD122 pKa = 3.87WIVSNLYY129 pKa = 8.64ATQEE133 pKa = 3.97RR134 pKa = 11.84AAGGVDD140 pKa = 4.48FISYY144 pKa = 10.67SKK146 pKa = 11.12VFDD149 pKa = 3.9GVLVAKK155 pKa = 10.06DD156 pKa = 3.68NPEE159 pKa = 4.72GITGIDD165 pKa = 3.23TSLCGLTVALNKK177 pKa = 10.64GYY179 pKa = 11.09VEE181 pKa = 4.46VPLVEE186 pKa = 5.54AVAPDD191 pKa = 3.76CTAAGLAAPEE201 pKa = 4.09VALFDD206 pKa = 4.06SSADD210 pKa = 3.52CVQAILAGRR219 pKa = 11.84ADD221 pKa = 4.08AYY223 pKa = 10.87MNDD226 pKa = 3.08INTVNRR232 pKa = 11.84FIAEE236 pKa = 4.24HH237 pKa = 7.14PDD239 pKa = 3.71DD240 pKa = 5.42LDD242 pKa = 3.65SAEE245 pKa = 4.31TVMLDD250 pKa = 3.25YY251 pKa = 11.0EE252 pKa = 4.21IGIGVLQGEE261 pKa = 4.66HH262 pKa = 6.04EE263 pKa = 4.43FRR265 pKa = 11.84DD266 pKa = 3.87AVQAALTVVQDD277 pKa = 3.44TGLQTEE283 pKa = 4.63LAEE286 pKa = 4.46KK287 pKa = 10.33WEE289 pKa = 4.29LDD291 pKa = 3.43ANAVAEE297 pKa = 4.22PTVLSVGG304 pKa = 3.6

Molecular weight: 31.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0V8T962|A0A0V8T962_9CELL Glycosyl transferase OS=Cellulomonas sp. B6 OX=1295626 GN=ATM99_08880 PE=4 SV=1
MM1 pKa = 7.51TKK3 pKa = 9.09RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.61GRR40 pKa = 11.84AEE42 pKa = 3.84LSAA45 pKa = 4.93

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3428

0

3428

1139415

37

2682

332.4

35.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.181 ± 0.075

0.54 ± 0.01

6.746 ± 0.038

4.876 ± 0.034

2.347 ± 0.027

9.392 ± 0.037

2.169 ± 0.025

2.311 ± 0.034

1.189 ± 0.026

10.439 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.438 ± 0.018

1.307 ± 0.023

6.042 ± 0.036

2.604 ± 0.022

8.152 ± 0.052

4.471 ± 0.028

6.517 ± 0.042

10.985 ± 0.052

1.565 ± 0.02

1.726 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski