Amino acid dipepetide frequency for Colwellia sp. MT41

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.148AlaAla: 7.148 ± 0.116
1.004AlaCys: 1.004 ± 0.03
4.767AlaAsp: 4.767 ± 0.072
5.289AlaGlu: 5.289 ± 0.076
3.343AlaPhe: 3.343 ± 0.058
5.56AlaGly: 5.56 ± 0.083
1.683AlaHis: 1.683 ± 0.041
6.776AlaIle: 6.776 ± 0.087
6.132AlaLys: 6.132 ± 0.089
9.56AlaLeu: 9.56 ± 0.109
2.4AlaMet: 2.4 ± 0.05
4.189AlaAsn: 4.189 ± 0.067
2.432AlaPro: 2.432 ± 0.056
4.329AlaGln: 4.329 ± 0.066
3.104AlaArg: 3.104 ± 0.053
5.677AlaSer: 5.677 ± 0.07
4.578AlaThr: 4.578 ± 0.081
5.395AlaVal: 5.395 ± 0.074
0.871AlaTrp: 0.871 ± 0.029
2.375AlaTyr: 2.375 ± 0.048
0.0AlaXaa: 0.0 ± 0.0
Cys
0.774CysAla: 0.774 ± 0.029
0.157CysCys: 0.157 ± 0.012
0.598CysAsp: 0.598 ± 0.022
0.48CysGlu: 0.48 ± 0.018
0.496CysPhe: 0.496 ± 0.022
0.8CysGly: 0.8 ± 0.027
0.375CysHis: 0.375 ± 0.016
0.673CysIle: 0.673 ± 0.024
0.455CysLys: 0.455 ± 0.019
1.08CysLeu: 1.08 ± 0.033
0.203CysMet: 0.203 ± 0.013
0.393CysAsn: 0.393 ± 0.02
0.466CysPro: 0.466 ± 0.023
0.716CysGln: 0.716 ± 0.024
0.421CysArg: 0.421 ± 0.018
0.712CysSer: 0.712 ± 0.024
0.479CysThr: 0.479 ± 0.021
0.537CysVal: 0.537 ± 0.023
0.127CysTrp: 0.127 ± 0.012
0.331CysTyr: 0.331 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
4.042AspAla: 4.042 ± 0.07
0.591AspCys: 0.591 ± 0.023
3.435AspAsp: 3.435 ± 0.075
3.827AspGlu: 3.827 ± 0.069
2.824AspPhe: 2.824 ± 0.051
3.581AspGly: 3.581 ± 0.101
1.074AspHis: 1.074 ± 0.029
4.823AspIle: 4.823 ± 0.072
4.07AspLys: 4.07 ± 0.061
5.169AspLeu: 5.169 ± 0.073
1.276AspMet: 1.276 ± 0.037
3.1AspAsn: 3.1 ± 0.057
1.532AspPro: 1.532 ± 0.041
1.519AspGln: 1.519 ± 0.031
1.78AspArg: 1.78 ± 0.038
3.626AspSer: 3.626 ± 0.066
2.499AspThr: 2.499 ± 0.049
3.562AspVal: 3.562 ± 0.057
0.697AspTrp: 0.697 ± 0.024
2.181AspTyr: 2.181 ± 0.048
0.0AspXaa: 0.0 ± 0.0
Glu
4.008GluAla: 4.008 ± 0.071
0.407GluCys: 0.407 ± 0.019
2.576GluAsp: 2.576 ± 0.05
2.939GluGlu: 2.939 ± 0.07
2.419GluPhe: 2.419 ± 0.045
2.763GluGly: 2.763 ± 0.046
1.627GluHis: 1.627 ± 0.041
4.153GluIle: 4.153 ± 0.065
3.937GluLys: 3.937 ± 0.064
6.766GluLeu: 6.766 ± 0.081
1.386GluMet: 1.386 ± 0.035
2.908GluAsn: 2.908 ± 0.061
1.594GluPro: 1.594 ± 0.036
4.643GluGln: 4.643 ± 0.068
2.473GluArg: 2.473 ± 0.051
3.153GluSer: 3.153 ± 0.059
2.564GluThr: 2.564 ± 0.051
3.846GluVal: 3.846 ± 0.062
0.493GluTrp: 0.493 ± 0.021
1.691GluTyr: 1.691 ± 0.043
0.0GluXaa: 0.0 ± 0.0
Phe
3.634PheAla: 3.634 ± 0.053
0.484PheCys: 0.484 ± 0.02
2.826PheAsp: 2.826 ± 0.045
2.249PheGlu: 2.249 ± 0.043
1.979PhePhe: 1.979 ± 0.053
2.802PheGly: 2.802 ± 0.048
0.834PheHis: 0.834 ± 0.028
3.541PheIle: 3.541 ± 0.059
2.441PheLys: 2.441 ± 0.046
3.399PheLeu: 3.399 ± 0.062
0.93PheMet: 0.93 ± 0.028
2.438PheAsn: 2.438 ± 0.044
1.354PhePro: 1.354 ± 0.037
1.205PheGln: 1.205 ± 0.037
1.31PheArg: 1.31 ± 0.039
3.9PheSer: 3.9 ± 0.065
2.881PheThr: 2.881 ± 0.058
2.55PheVal: 2.55 ± 0.05
0.501PheTrp: 0.501 ± 0.019
1.545PheTyr: 1.545 ± 0.042
0.0PheXaa: 0.0 ± 0.0
Gly
4.721GlyAla: 4.721 ± 0.079
0.791GlyCys: 0.791 ± 0.029
3.602GlyAsp: 3.602 ± 0.07
3.808GlyGlu: 3.808 ± 0.06
3.198GlyPhe: 3.198 ± 0.056
4.23GlyGly: 4.23 ± 0.082
1.483GlyHis: 1.483 ± 0.038
4.689GlyIle: 4.689 ± 0.067
4.205GlyLys: 4.205 ± 0.066
6.323GlyLeu: 6.323 ± 0.069
1.626GlyMet: 1.626 ± 0.046
2.728GlyAsn: 2.728 ± 0.055
1.394GlyPro: 1.394 ± 0.033
2.701GlyGln: 2.701 ± 0.046
2.59GlyArg: 2.59 ± 0.049
3.898GlySer: 3.898 ± 0.072
3.124GlyThr: 3.124 ± 0.065
4.301GlyVal: 4.301 ± 0.069
0.734GlyTrp: 0.734 ± 0.026
2.412GlyTyr: 2.412 ± 0.046
0.0GlyXaa: 0.0 ± 0.0
His
1.58HisAla: 1.58 ± 0.036
0.389HisCys: 0.389 ± 0.018
1.15HisAsp: 1.15 ± 0.038
1.085HisGlu: 1.085 ± 0.028
1.156HisPhe: 1.156 ± 0.031
1.522HisGly: 1.522 ± 0.041
0.663HisHis: 0.663 ± 0.025
1.596HisIle: 1.596 ± 0.04
1.253HisLys: 1.253 ± 0.033
2.216HisLeu: 2.216 ± 0.048
0.46HisMet: 0.46 ± 0.018
1.045HisAsn: 1.045 ± 0.028
0.902HisPro: 0.902 ± 0.029
1.331HisGln: 1.331 ± 0.034
0.933HisArg: 0.933 ± 0.028
1.493HisSer: 1.493 ± 0.034
1.029HisThr: 1.029 ± 0.033
1.212HisVal: 1.212 ± 0.035
0.368HisTrp: 0.368 ± 0.015
0.918HisTyr: 0.918 ± 0.034
0.0HisXaa: 0.0 ± 0.0
Ile
7.319IleAla: 7.319 ± 0.087
0.739IleCys: 0.739 ± 0.028
4.933IleAsp: 4.933 ± 0.072
4.894IleGlu: 4.894 ± 0.073
2.743IlePhe: 2.743 ± 0.054
4.407IleGly: 4.407 ± 0.064
1.39IleHis: 1.39 ± 0.032
5.028IleIle: 5.028 ± 0.079
4.681IleLys: 4.681 ± 0.067
5.858IleLeu: 5.858 ± 0.094
1.417IleMet: 1.417 ± 0.039
4.127IleAsn: 4.127 ± 0.063
2.491IlePro: 2.491 ± 0.05
2.14IleGln: 2.14 ± 0.044
2.587IleArg: 2.587 ± 0.048
5.744IleSer: 5.744 ± 0.077
4.658IleThr: 4.658 ± 0.066
4.181IleVal: 4.181 ± 0.067
0.597IleTrp: 0.597 ± 0.022
2.019IleTyr: 2.019 ± 0.045
0.0IleXaa: 0.0 ± 0.0
Lys
5.423LysAla: 5.423 ± 0.08
0.391LysCys: 0.391 ± 0.019
2.921LysAsp: 2.921 ± 0.059
3.468LysGlu: 3.468 ± 0.065
1.983LysPhe: 1.983 ± 0.044
3.359LysGly: 3.359 ± 0.056
1.441LysHis: 1.441 ± 0.038
4.394LysIle: 4.394 ± 0.072
4.104LysLys: 4.104 ± 0.073
6.25LysLeu: 6.25 ± 0.079
1.526LysMet: 1.526 ± 0.04
3.216LysAsn: 3.216 ± 0.058
2.324LysPro: 2.324 ± 0.046
3.567LysGln: 3.567 ± 0.073
2.713LysArg: 2.713 ± 0.054
4.04LysSer: 4.04 ± 0.061
3.638LysThr: 3.638 ± 0.053
4.449LysVal: 4.449 ± 0.064
0.529LysTrp: 0.529 ± 0.021
1.792LysTyr: 1.792 ± 0.042
0.0LysXaa: 0.0 ± 0.0
Leu
11.262LeuAla: 11.262 ± 0.123
1.0LeuCys: 1.0 ± 0.027
5.305LeuAsp: 5.305 ± 0.083
4.889LeuGlu: 4.889 ± 0.074
4.261LeuPhe: 4.261 ± 0.073
6.261LeuGly: 6.261 ± 0.086
1.976LeuHis: 1.976 ± 0.049
6.857LeuIle: 6.857 ± 0.103
5.72LeuLys: 5.72 ± 0.086
11.038LeuLeu: 11.038 ± 0.143
2.573LeuMet: 2.573 ± 0.051
4.999LeuAsn: 4.999 ± 0.071
4.423LeuPro: 4.423 ± 0.067
3.71LeuGln: 3.71 ± 0.067
3.813LeuArg: 3.813 ± 0.058
8.469LeuSer: 8.469 ± 0.105
7.074LeuThr: 7.074 ± 0.08
6.925LeuVal: 6.925 ± 0.094
1.002LeuTrp: 1.002 ± 0.033
2.789LeuTyr: 2.789 ± 0.05
0.0LeuXaa: 0.0 ± 0.0
Met
2.443MetAla: 2.443 ± 0.053
0.186MetCys: 0.186 ± 0.013
1.061MetAsp: 1.061 ± 0.033
0.956MetGlu: 0.956 ± 0.034
0.881MetPhe: 0.881 ± 0.03
1.417MetGly: 1.417 ± 0.037
0.472MetHis: 0.472 ± 0.021
1.435MetIle: 1.435 ± 0.038
1.471MetLys: 1.471 ± 0.037
2.58MetLeu: 2.58 ± 0.052
0.69MetMet: 0.69 ± 0.03
1.064MetAsn: 1.064 ± 0.028
1.253MetPro: 1.253 ± 0.029
1.093MetGln: 1.093 ± 0.035
0.934MetArg: 0.934 ± 0.032
1.871MetSer: 1.871 ± 0.043
1.505MetThr: 1.505 ± 0.036
1.574MetVal: 1.574 ± 0.038
0.18MetTrp: 0.18 ± 0.013
0.533MetTyr: 0.533 ± 0.021
0.0MetXaa: 0.0 ± 0.0
Asn
3.654AsnAla: 3.654 ± 0.071
0.495AsnCys: 0.495 ± 0.022
2.82AsnAsp: 2.82 ± 0.054
2.774AsnGlu: 2.774 ± 0.047
1.996AsnPhe: 1.996 ± 0.045
2.975AsnGly: 2.975 ± 0.067
1.091AsnHis: 1.091 ± 0.033
3.955AsnIle: 3.955 ± 0.057
3.572AsnLys: 3.572 ± 0.061
4.435AsnLeu: 4.435 ± 0.064
1.052AsnMet: 1.052 ± 0.029
3.007AsnAsn: 3.007 ± 0.063
1.896AsnPro: 1.896 ± 0.051
2.111AsnGln: 2.111 ± 0.044
1.806AsnArg: 1.806 ± 0.039
3.423AsnSer: 3.423 ± 0.069
2.804AsnThr: 2.804 ± 0.052
2.69AsnVal: 2.69 ± 0.048
0.612AsnTrp: 0.612 ± 0.021
1.702AsnTyr: 1.702 ± 0.041
0.0AsnXaa: 0.0 ± 0.0
Pro
2.785ProAla: 2.785 ± 0.059
0.308ProCys: 0.308 ± 0.016
1.942ProAsp: 1.942 ± 0.047
2.595ProGlu: 2.595 ± 0.05
1.574ProPhe: 1.574 ± 0.038
2.01ProGly: 2.01 ± 0.047
0.754ProHis: 0.754 ± 0.027
2.412ProIle: 2.412 ± 0.054
1.985ProLys: 1.985 ± 0.042
3.859ProLeu: 3.859 ± 0.061
0.893ProMet: 0.893 ± 0.029
1.574ProAsn: 1.574 ± 0.041
0.96ProPro: 0.96 ± 0.028
1.42ProGln: 1.42 ± 0.034
1.174ProArg: 1.174 ± 0.034
2.374ProSer: 2.374 ± 0.047
1.846ProThr: 1.846 ± 0.041
2.55ProVal: 2.55 ± 0.043
0.471ProTrp: 0.471 ± 0.021
1.184ProTyr: 1.184 ± 0.035
0.0ProXaa: 0.0 ± 0.0
Gln
5.022GlnAla: 5.022 ± 0.081
0.432GlnCys: 0.432 ± 0.021
2.109GlnAsp: 2.109 ± 0.04
2.382GlnGlu: 2.382 ± 0.052
1.688GlnPhe: 1.688 ± 0.038
3.438GlnGly: 3.438 ± 0.062
1.293GlnHis: 1.293 ± 0.04
2.569GlnIle: 2.569 ± 0.041
2.339GlnLys: 2.339 ± 0.05
5.832GlnLeu: 5.832 ± 0.091
0.94GlnMet: 0.94 ± 0.034
1.618GlnAsn: 1.618 ± 0.04
1.522GlnPro: 1.522 ± 0.037
3.967GlnGln: 3.967 ± 0.094
2.066GlnArg: 2.066 ± 0.045
2.8GlnSer: 2.8 ± 0.059
2.195GlnThr: 2.195 ± 0.042
3.585GlnVal: 3.585 ± 0.06
0.666GlnTrp: 0.666 ± 0.025
1.502GlnTyr: 1.502 ± 0.04
0.0GlnXaa: 0.0 ± 0.0
Arg
3.067ArgAla: 3.067 ± 0.056
0.426ArgCys: 0.426 ± 0.019
2.085ArgAsp: 2.085 ± 0.051
2.2ArgGlu: 2.2 ± 0.046
1.896ArgPhe: 1.896 ± 0.04
2.224ArgGly: 2.224 ± 0.042
0.932ArgHis: 0.932 ± 0.032
2.802ArgIle: 2.802 ± 0.047
2.23ArgLys: 2.23 ± 0.047
4.329ArgLeu: 4.329 ± 0.07
0.924ArgMet: 0.924 ± 0.027
1.699ArgAsn: 1.699 ± 0.037
1.254ArgPro: 1.254 ± 0.03
2.048ArgGln: 2.048 ± 0.051
1.78ArgArg: 1.78 ± 0.043
2.255ArgSer: 2.255 ± 0.042
1.764ArgThr: 1.764 ± 0.042
2.606ArgVal: 2.606 ± 0.05
0.489ArgTrp: 0.489 ± 0.02
1.572ArgTyr: 1.572 ± 0.037
0.0ArgXaa: 0.0 ± 0.0
Ser
6.1SerAla: 6.1 ± 0.077
0.737SerCys: 0.737 ± 0.03
3.821SerAsp: 3.821 ± 0.062
3.779SerGlu: 3.779 ± 0.063
3.331SerPhe: 3.331 ± 0.051
4.677SerGly: 4.677 ± 0.084
1.596SerHis: 1.596 ± 0.041
4.686SerIle: 4.686 ± 0.069
3.998SerLys: 3.998 ± 0.07
7.521SerLeu: 7.521 ± 0.092
1.581SerMet: 1.581 ± 0.038
3.284SerAsn: 3.284 ± 0.067
2.428SerPro: 2.428 ± 0.051
3.329SerGln: 3.329 ± 0.064
2.642SerArg: 2.642 ± 0.053
4.849SerSer: 4.849 ± 0.077
3.702SerThr: 3.702 ± 0.061
4.323SerVal: 4.323 ± 0.064
0.839SerTrp: 0.839 ± 0.027
2.453SerTyr: 2.453 ± 0.051
0.0SerXaa: 0.0 ± 0.0
Thr
4.852ThrAla: 4.852 ± 0.072
0.514ThrCys: 0.514 ± 0.02
3.23ThrAsp: 3.23 ± 0.061
3.225ThrGlu: 3.225 ± 0.057
2.177ThrPhe: 2.177 ± 0.045
3.933ThrGly: 3.933 ± 0.069
1.099ThrHis: 1.099 ± 0.031
3.995ThrIle: 3.995 ± 0.059
2.948ThrLys: 2.948 ± 0.051
6.366ThrLeu: 6.366 ± 0.086
1.225ThrMet: 1.225 ± 0.034
2.41ThrAsn: 2.41 ± 0.053
2.523ThrPro: 2.523 ± 0.048
2.226ThrGln: 2.226 ± 0.036
1.985ThrArg: 1.985 ± 0.042
3.764ThrSer: 3.764 ± 0.061
3.15ThrThr: 3.15 ± 0.062
3.613ThrVal: 3.613 ± 0.064
0.603ThrTrp: 0.603 ± 0.025
1.501ThrTyr: 1.501 ± 0.04
0.0ThrXaa: 0.0 ± 0.0
Val
5.842ValAla: 5.842 ± 0.085
0.666ValCys: 0.666 ± 0.023
3.875ValAsp: 3.875 ± 0.061
3.825ValGlu: 3.825 ± 0.067
2.732ValPhe: 2.732 ± 0.054
3.863ValGly: 3.863 ± 0.072
1.19ValHis: 1.19 ± 0.039
5.132ValIle: 5.132 ± 0.073
4.018ValLys: 4.018 ± 0.061
6.328ValLeu: 6.328 ± 0.08
1.665ValMet: 1.665 ± 0.042
3.408ValAsn: 3.408 ± 0.053
2.217ValPro: 2.217 ± 0.05
2.095ValGln: 2.095 ± 0.043
2.298ValArg: 2.298 ± 0.05
4.656ValSer: 4.656 ± 0.069
3.931ValThr: 3.931 ± 0.067
4.369ValVal: 4.369 ± 0.073
0.615ValTrp: 0.615 ± 0.023
1.784ValTyr: 1.784 ± 0.041
0.0ValXaa: 0.0 ± 0.0
Trp
0.686TrpAla: 0.686 ± 0.025
0.148TrpCys: 0.148 ± 0.012
0.484TrpAsp: 0.484 ± 0.023
0.357TrpGlu: 0.357 ± 0.019
0.544TrpPhe: 0.544 ± 0.025
0.663TrpGly: 0.663 ± 0.023
0.421TrpHis: 0.421 ± 0.02
0.469TrpIle: 0.469 ± 0.022
0.346TrpLys: 0.346 ± 0.018
1.771TrpLeu: 1.771 ± 0.045
0.212TrpMet: 0.212 ± 0.015
0.342TrpAsn: 0.342 ± 0.019
0.428TrpPro: 0.428 ± 0.02
1.3TrpGln: 1.3 ± 0.039
0.58TrpArg: 0.58 ± 0.023
0.71TrpSer: 0.71 ± 0.022
0.408TrpThr: 0.408 ± 0.021
0.627TrpVal: 0.627 ± 0.027
0.157TrpTrp: 0.157 ± 0.011
0.356TrpTyr: 0.356 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.249TyrAla: 2.249 ± 0.043
0.408TyrCys: 0.408 ± 0.02
1.667TyrAsp: 1.667 ± 0.039
1.324TyrGlu: 1.324 ± 0.039
1.585TyrPhe: 1.585 ± 0.038
2.077TyrGly: 2.077 ± 0.051
0.906TyrHis: 0.906 ± 0.025
1.97TyrIle: 1.97 ± 0.043
1.619TyrLys: 1.619 ± 0.037
3.665TyrLeu: 3.665 ± 0.071
0.617TyrMet: 0.617 ± 0.021
1.322TyrAsn: 1.322 ± 0.035
1.277TyrPro: 1.277 ± 0.034
2.513TyrGln: 2.513 ± 0.058
1.596TyrArg: 1.596 ± 0.038
2.261TyrSer: 2.261 ± 0.049
1.511TyrThr: 1.511 ± 0.039
1.614TyrVal: 1.614 ± 0.037
0.459TyrTrp: 0.459 ± 0.017
1.215TyrTyr: 1.215 ± 0.034
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3491 proteins (1168266 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski