Bhendi yellow vein mosaic alphasatellite
Average proteome isoelectric point is 8.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0F6QR00|A0A0F6QR00_9VIRU Replication protein OS=Bhendi yellow vein mosaic alphasatellite OX=759124 PE=4 SV=1
MM1 pKa = 7.51 PTIQSQWWCFTVFFLSATAPDD22 pKa = 4.4 LVPLFEE28 pKa = 4.5 NTHH31 pKa = 4.91 VSYY34 pKa = 11.22 ACWQEE39 pKa = 4.11 EE40 pKa = 4.2 EE41 pKa = 4.78 SPTTRR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 HH49 pKa = 4.54 LQGYY53 pKa = 8.3 LQLKK57 pKa = 8.77 GKK59 pKa = 10.49 RR60 pKa = 11.84 SLAQVKK66 pKa = 10.33 ALFGDD71 pKa = 4.58 LNPHH75 pKa = 6.48 LEE77 pKa = 4.05 KK78 pKa = 10.69 QRR80 pKa = 11.84 ARR82 pKa = 11.84 KK83 pKa = 7.55 TDD85 pKa = 3.28 EE86 pKa = 3.84 ARR88 pKa = 11.84 DD89 pKa = 3.66 YY90 pKa = 11.56 CMKK93 pKa = 10.74 EE94 pKa = 3.85 EE95 pKa = 4.25 TRR97 pKa = 11.84 VSGPFEE103 pKa = 4.18 FGEE106 pKa = 4.19 YY107 pKa = 8.47 TAAGSHH113 pKa = 6.03 KK114 pKa = 9.52 RR115 pKa = 11.84 RR116 pKa = 11.84 QRR118 pKa = 11.84 EE119 pKa = 3.99 SVIRR123 pKa = 11.84 SPVRR127 pKa = 11.84 MAEE130 pKa = 4.07 EE131 pKa = 4.09 NPSVFRR137 pKa = 11.84 RR138 pKa = 11.84 VKK140 pKa = 10.82 GKK142 pKa = 9.39 DD143 pKa = 2.91 CRR145 pKa = 11.84 RR146 pKa = 11.84 RR147 pKa = 11.84 FPEE150 pKa = 4.23 DD151 pKa = 3.17 RR152 pKa = 11.84 EE153 pKa = 4.2
Molecular weight: 18.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.949
IPC2_protein 8.697
IPC_protein 8.726
Toseland 9.37
ProMoST 9.18
Dawson 9.648
Bjellqvist 9.472
Wikipedia 9.794
Rodwell 9.853
Grimsley 9.677
Solomon 9.736
Lehninger 9.706
Nozaki 9.663
DTASelect 9.37
Thurlkill 9.516
EMBOSS 9.809
Sillero 9.663
Patrickios 5.525
IPC_peptide 9.736
IPC2_peptide 8.361
IPC2.peptide.svr19 7.769
Protein with the highest isoelectric point:
>tr|A0A0F6QR00|A0A0F6QR00_9VIRU Replication protein OS=Bhendi yellow vein mosaic alphasatellite OX=759124 PE=4 SV=1
MM1 pKa = 7.51 PTIQSQWWCFTVFFLSATAPDD22 pKa = 4.4 LVPLFEE28 pKa = 4.5 NTHH31 pKa = 4.91 VSYY34 pKa = 11.22 ACWQEE39 pKa = 4.11 EE40 pKa = 4.2 EE41 pKa = 4.78 SPTTRR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 HH49 pKa = 4.54 LQGYY53 pKa = 8.3 LQLKK57 pKa = 8.77 GKK59 pKa = 10.49 RR60 pKa = 11.84 SLAQVKK66 pKa = 10.33 ALFGDD71 pKa = 4.58 LNPHH75 pKa = 6.48 LEE77 pKa = 4.05 KK78 pKa = 10.69 QRR80 pKa = 11.84 ARR82 pKa = 11.84 KK83 pKa = 7.55 TDD85 pKa = 3.28 EE86 pKa = 3.84 ARR88 pKa = 11.84 DD89 pKa = 3.66 YY90 pKa = 11.56 CMKK93 pKa = 10.74 EE94 pKa = 3.85 EE95 pKa = 4.25 TRR97 pKa = 11.84 VSGPFEE103 pKa = 4.18 FGEE106 pKa = 4.19 YY107 pKa = 8.47 TAAGSHH113 pKa = 6.03 KK114 pKa = 9.52 RR115 pKa = 11.84 RR116 pKa = 11.84 QRR118 pKa = 11.84 EE119 pKa = 3.99 SVIRR123 pKa = 11.84 SPVRR127 pKa = 11.84 MAEE130 pKa = 4.07 EE131 pKa = 4.09 NPSVFRR137 pKa = 11.84 RR138 pKa = 11.84 VKK140 pKa = 10.82 GKK142 pKa = 9.39 DD143 pKa = 2.91 CRR145 pKa = 11.84 RR146 pKa = 11.84 RR147 pKa = 11.84 FPEE150 pKa = 4.23 DD151 pKa = 3.17 RR152 pKa = 11.84 EE153 pKa = 4.2
Molecular weight: 18.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.949
IPC2_protein 8.697
IPC_protein 8.726
Toseland 9.37
ProMoST 9.18
Dawson 9.648
Bjellqvist 9.472
Wikipedia 9.794
Rodwell 9.853
Grimsley 9.677
Solomon 9.736
Lehninger 9.706
Nozaki 9.663
DTASelect 9.37
Thurlkill 9.516
EMBOSS 9.809
Sillero 9.663
Patrickios 5.525
IPC_peptide 9.736
IPC2_peptide 8.361
IPC2.peptide.svr19 7.769
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
153
153
153
153.0
18.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.536 ± 0.0
2.614 ± 0.0
3.922 ± 0.0
9.804 ± 0.0
5.882 ± 0.0
4.575 ± 0.0
2.614 ± 0.0
1.307 ± 0.0
5.882 ± 0.0
6.536 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.961 ± 0.0
1.961 ± 0.0
5.882 ± 0.0
5.229 ± 0.0
12.418 ± 0.0
6.536 ± 0.0
5.882 ± 0.0
5.882 ± 0.0
1.961 ± 0.0
2.614 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here