Pseudoalteromonas phage Pq0

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H4A748|A0A0H4A748_9CAUD Uncharacterized protein OS=Pseudoalteromonas phage Pq0 OX=1667322 PE=4 SV=1
MM1 pKa = 7.34TPAEE5 pKa = 4.51YY6 pKa = 10.47LVLWDD11 pKa = 3.65YY12 pKa = 11.83SYY14 pKa = 11.31ATDD17 pKa = 3.62PAYY20 pKa = 10.51AEE22 pKa = 3.83QRR24 pKa = 11.84KK25 pKa = 9.51ASEE28 pKa = 4.09LDD30 pKa = 3.11EE31 pKa = 4.86ALIEE35 pKa = 4.17NWDD38 pKa = 3.73LVLL41 pKa = 5.57

Molecular weight: 4.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H4ABG9|A0A0H4ABG9_9CAUD GIY-YIG domain-containing protein OS=Pseudoalteromonas phage Pq0 OX=1667322 PE=4 SV=1
MM1 pKa = 7.24TRR3 pKa = 11.84LITKK7 pKa = 7.52QMRR10 pKa = 11.84DD11 pKa = 3.3SFTSRR16 pKa = 11.84WWNCTEE22 pKa = 3.83AKK24 pKa = 9.56KK25 pKa = 10.36KK26 pKa = 10.2QRR28 pKa = 11.84ILILKK33 pKa = 8.4RR34 pKa = 11.84TMITLQSKK42 pKa = 9.92GLITFTQTVEE52 pKa = 3.89KK53 pKa = 11.24GEE55 pKa = 4.24GKK57 pKa = 10.29LGKK60 pKa = 9.97SS61 pKa = 3.49

Molecular weight: 7.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

10024

37

711

179.0

19.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.47 ± 0.638

1.077 ± 0.136

6.155 ± 0.282

6.874 ± 0.358

3.611 ± 0.181

6.604 ± 0.416

1.267 ± 0.159

6.395 ± 0.177

7.322 ± 0.534

8.15 ± 0.315

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.723 ± 0.297

5.756 ± 0.244

3.003 ± 0.255

4.25 ± 0.313

4.469 ± 0.221

7.273 ± 0.466

5.716 ± 0.365

6.205 ± 0.254

1.277 ± 0.119

3.402 ± 0.222
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski