Babesia ovata

Taxonomy: cellular organisms; Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Piroplasmida; Babesiidae; Babesia

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5006 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2H6KE28|A0A2H6KE28_9APIC Uncharacterized protein OS=Babesia ovata OX=189622 GN=BOVATA_027270 PE=4 SV=1
MM1 pKa = 7.57AALSGIPLVPTWGDD15 pKa = 3.46SFAVSCDD22 pKa = 3.49LDD24 pKa = 3.92LLLFADD30 pKa = 4.81APRR33 pKa = 11.84DD34 pKa = 3.45VTDD37 pKa = 4.29FVEE40 pKa = 4.5SLPPRR45 pKa = 11.84PSSLYY50 pKa = 10.23SLNAASSFSVKK61 pKa = 10.14RR62 pKa = 11.84PMPVSSADD70 pKa = 3.33PCFLLVSGPSEE81 pKa = 4.19SSALGLSSSKK91 pKa = 10.96LLDD94 pKa = 3.81FPGLLLDD101 pKa = 4.48SPFAAAPLLPPPLLEE116 pKa = 5.4DD117 pKa = 4.32GLWGCPEE124 pKa = 4.41SYY126 pKa = 11.21ASLAEE131 pKa = 4.29SFSSDD136 pKa = 3.84TISLSLDD143 pKa = 3.34PPIPDD148 pKa = 4.53LPLSTPVPAFAFSAANMASLMDD170 pKa = 3.77SCVFAAVDD178 pKa = 3.69TAGVTSPFCCVPGRR192 pKa = 11.84RR193 pKa = 11.84NFAA196 pKa = 3.59

Molecular weight:
20.31 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2H6KF46|A0A2H6KF46_9APIC Elongation factor TS OS=Babesia ovata OX=189622 GN=BOVATA_030870 PE=3 SV=1
MM1 pKa = 7.62ASAHH5 pKa = 5.29QLEE8 pKa = 4.58QFFVEE13 pKa = 4.55ALRR16 pKa = 11.84HH17 pKa = 5.44RR18 pKa = 11.84GSRR21 pKa = 11.84GHH23 pKa = 6.85AARR26 pKa = 11.84RR27 pKa = 11.84DD28 pKa = 3.58QEE30 pKa = 3.99RR31 pKa = 11.84QRR33 pKa = 11.84SKK35 pKa = 10.52RR36 pKa = 11.84HH37 pKa = 4.79HH38 pKa = 6.8HH39 pKa = 5.76GQQPVDD45 pKa = 4.11DD46 pKa = 4.7GVQQRR51 pKa = 11.84VSFALGSATIAISEE65 pKa = 4.33ALRR68 pKa = 11.84HH69 pKa = 4.97VEE71 pKa = 3.66RR72 pKa = 11.84EE73 pKa = 3.54RR74 pKa = 11.84RR75 pKa = 11.84KK76 pKa = 10.33RR77 pKa = 11.84NGGKK81 pKa = 10.21VNIPVGDD88 pKa = 3.8RR89 pKa = 11.84CDD91 pKa = 3.1GAMRR95 pKa = 11.84IRR97 pKa = 11.84LDD99 pKa = 3.57EE100 pKa = 4.55PPGEE104 pKa = 4.25FAQEE108 pKa = 3.76RR109 pKa = 11.84VRR111 pKa = 11.84CIGRR115 pKa = 11.84EE116 pKa = 3.76VDD118 pKa = 3.77VKK120 pKa = 11.25LLVLVALGVRR130 pKa = 11.84QYY132 pKa = 11.02GVNIGVSRR140 pKa = 11.84ADD142 pKa = 3.08KK143 pKa = 10.8VYY145 pKa = 10.44GVHH148 pKa = 6.56PRR150 pKa = 11.84YY151 pKa = 10.11GLPTVRR157 pKa = 11.84YY158 pKa = 8.54RR159 pKa = 11.84HH160 pKa = 6.11IEE162 pKa = 3.96GHH164 pKa = 5.75SEE166 pKa = 3.62AAQFTHH172 pKa = 6.08FQYY175 pKa = 10.95RR176 pKa = 3.29

Molecular weight:
20.07 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5006

0

5006

3226480

66

7113

644.5

71.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.457 ± 0.026

2.057 ± 0.015

6.218 ± 0.019

6.346 ± 0.027

3.499 ± 0.021

6.354 ± 0.025

2.745 ± 0.015

4.97 ± 0.019

6.583 ± 0.059

9.685 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.036 ± 0.018

4.548 ± 0.024

4.447 ± 0.035

4.01 ± 0.02

5.307 ± 0.034

7.592 ± 0.032

5.641 ± 0.02

6.597 ± 0.028

0.928 ± 0.008

2.978 ± 0.015

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski