Babesia ovata
Average proteome isoelectric point is 6.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5006 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H6KE28|A0A2H6KE28_9APIC Uncharacterized protein OS=Babesia ovata OX=189622 GN=BOVATA_027270 PE=4 SV=1
MM1 pKa = 7.57 AALSGIPLVPTWGDD15 pKa = 3.46 SFAVSCDD22 pKa = 3.49 LDD24 pKa = 3.92 LLLFADD30 pKa = 4.81 APRR33 pKa = 11.84 DD34 pKa = 3.45 VTDD37 pKa = 4.29 FVEE40 pKa = 4.5 SLPPRR45 pKa = 11.84 PSSLYY50 pKa = 10.23 SLNAASSFSVKK61 pKa = 10.14 RR62 pKa = 11.84 PMPVSSADD70 pKa = 3.33 PCFLLVSGPSEE81 pKa = 4.19 SSALGLSSSKK91 pKa = 10.96 LLDD94 pKa = 3.81 FPGLLLDD101 pKa = 4.48 SPFAAAPLLPPPLLEE116 pKa = 5.4 DD117 pKa = 4.32 GLWGCPEE124 pKa = 4.41 SYY126 pKa = 11.21 ASLAEE131 pKa = 4.29 SFSSDD136 pKa = 3.84 TISLSLDD143 pKa = 3.34 PPIPDD148 pKa = 4.53 LPLSTPVPAFAFSAANMASLMDD170 pKa = 3.77 SCVFAAVDD178 pKa = 3.69 TAGVTSPFCCVPGRR192 pKa = 11.84 RR193 pKa = 11.84 NFAA196 pKa = 3.59
Molecular weight: 20.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.725
IPC2_protein 3.732
IPC_protein 3.732
Toseland 3.503
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.719
Rodwell 3.554
Grimsley 3.414
Solomon 3.719
Lehninger 3.681
Nozaki 3.859
DTASelect 4.139
Thurlkill 3.579
EMBOSS 3.719
Sillero 3.859
Patrickios 1.888
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.761
Protein with the highest isoelectric point:
>tr|A0A2H6KF46|A0A2H6KF46_9APIC Elongation factor TS OS=Babesia ovata OX=189622 GN=BOVATA_030870 PE=3 SV=1
MM1 pKa = 7.62 ASAHH5 pKa = 5.29 QLEE8 pKa = 4.58 QFFVEE13 pKa = 4.55 ALRR16 pKa = 11.84 HH17 pKa = 5.44 RR18 pKa = 11.84 GSRR21 pKa = 11.84 GHH23 pKa = 6.85 AARR26 pKa = 11.84 RR27 pKa = 11.84 DD28 pKa = 3.58 QEE30 pKa = 3.99 RR31 pKa = 11.84 QRR33 pKa = 11.84 SKK35 pKa = 10.52 RR36 pKa = 11.84 HH37 pKa = 4.79 HH38 pKa = 6.8 HH39 pKa = 5.76 GQQPVDD45 pKa = 4.11 DD46 pKa = 4.7 GVQQRR51 pKa = 11.84 VSFALGSATIAISEE65 pKa = 4.33 ALRR68 pKa = 11.84 HH69 pKa = 4.97 VEE71 pKa = 3.66 RR72 pKa = 11.84 EE73 pKa = 3.54 RR74 pKa = 11.84 RR75 pKa = 11.84 KK76 pKa = 10.33 RR77 pKa = 11.84 NGGKK81 pKa = 10.21 VNIPVGDD88 pKa = 3.8 RR89 pKa = 11.84 CDD91 pKa = 3.1 GAMRR95 pKa = 11.84 IRR97 pKa = 11.84 LDD99 pKa = 3.57 EE100 pKa = 4.55 PPGEE104 pKa = 4.25 FAQEE108 pKa = 3.76 RR109 pKa = 11.84 VRR111 pKa = 11.84 CIGRR115 pKa = 11.84 EE116 pKa = 3.76 VDD118 pKa = 3.77 VKK120 pKa = 11.25 LLVLVALGVRR130 pKa = 11.84 QYY132 pKa = 11.02 GVNIGVSRR140 pKa = 11.84 ADD142 pKa = 3.08 KK143 pKa = 10.8 VYY145 pKa = 10.44 GVHH148 pKa = 6.56 PRR150 pKa = 11.84 YY151 pKa = 10.11 GLPTVRR157 pKa = 11.84 YY158 pKa = 8.54 RR159 pKa = 11.84 HH160 pKa = 6.11 IEE162 pKa = 3.96 GHH164 pKa = 5.75 SEE166 pKa = 3.62 AAQFTHH172 pKa = 6.08 FQYY175 pKa = 10.95 RR176 pKa = 3.29
Molecular weight: 20.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.344
IPC2_protein 9.56
IPC_protein 10.599
Toseland 10.526
ProMoST 10.599
Dawson 10.657
Bjellqvist 10.452
Wikipedia 10.935
Rodwell 10.672
Grimsley 10.73
Solomon 10.804
Lehninger 10.76
Nozaki 10.526
DTASelect 10.438
Thurlkill 10.555
EMBOSS 10.95
Sillero 10.599
Patrickios 10.35
IPC_peptide 10.804
IPC2_peptide 9.604
IPC2.peptide.svr19 8.724
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5006
0
5006
3226480
66
7113
644.5
71.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.457 ± 0.026
2.057 ± 0.015
6.218 ± 0.019
6.346 ± 0.027
3.499 ± 0.021
6.354 ± 0.025
2.745 ± 0.015
4.97 ± 0.019
6.583 ± 0.059
9.685 ± 0.03
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.036 ± 0.018
4.548 ± 0.024
4.447 ± 0.035
4.01 ± 0.02
5.307 ± 0.034
7.592 ± 0.032
5.641 ± 0.02
6.597 ± 0.028
0.928 ± 0.008
2.978 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here