Mycobacterium phage Aggie

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Nclasvirinae; Charlievirus; unclassified Charlievirus

Average proteome isoelectric point is 5.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385D0L8|A0A385D0L8_9CAUD Tail terminator OS=Mycobacterium phage Aggie OX=2301547 GN=12 PE=4 SV=1
MM1 pKa = 7.73AKK3 pKa = 10.28LDD5 pKa = 3.88TLTDD9 pKa = 3.76DD10 pKa = 3.74FTVYY14 pKa = 10.9DD15 pKa = 4.1PGKK18 pKa = 7.82WTIDD22 pKa = 3.45DD23 pKa = 4.19SVGGDD28 pKa = 3.05IDD30 pKa = 4.0IGDD33 pKa = 3.53GRR35 pKa = 11.84AAINTNAGIEE45 pKa = 4.51GVFSVGAYY53 pKa = 10.22DD54 pKa = 4.68LLEE57 pKa = 4.63SYY59 pKa = 11.22AFAQFISFGFLYY71 pKa = 9.7PGKK74 pKa = 10.79GFGFSLNINSGGPNEE89 pKa = 4.37DD90 pKa = 2.86SAMFLCTEE98 pKa = 4.28SEE100 pKa = 4.55TEE102 pKa = 3.85GEE104 pKa = 4.27YY105 pKa = 11.3NLICLDD111 pKa = 3.54GAGFGAEE118 pKa = 4.19GTPIEE123 pKa = 4.4PVATGVTYY131 pKa = 10.74DD132 pKa = 3.82PAIHH136 pKa = 5.58SWLRR140 pKa = 11.84IRR142 pKa = 11.84EE143 pKa = 4.28SGGTIYY149 pKa = 10.47WEE151 pKa = 4.34TSPDD155 pKa = 3.6TAPLEE160 pKa = 4.22WSVMASGAHH169 pKa = 5.76TFTSAQATVQLGGVFAPAAFTVSSFNIAPTPPIPANAGNFFAFFF213 pKa = 4.14

Molecular weight: 22.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385D0J3|A0A385D0J3_9CAUD Uncharacterized protein OS=Mycobacterium phage Aggie OX=2301547 GN=1 PE=4 SV=1
MM1 pKa = 7.08STTPRR6 pKa = 11.84RR7 pKa = 11.84KK8 pKa = 9.92AKK10 pKa = 9.71TSARR14 pKa = 11.84GYY16 pKa = 9.55GAAHH20 pKa = 4.93QRR22 pKa = 11.84LRR24 pKa = 11.84EE25 pKa = 4.06KK26 pKa = 10.72YY27 pKa = 8.35RR28 pKa = 11.84QLVLSGRR35 pKa = 11.84ATCWRR40 pKa = 11.84CNQPIAPTEE49 pKa = 4.0EE50 pKa = 3.89WDD52 pKa = 4.91LGHH55 pKa = 7.8DD56 pKa = 3.81DD57 pKa = 5.74DD58 pKa = 6.79DD59 pKa = 3.77RR60 pKa = 11.84TKK62 pKa = 11.11YY63 pKa = 10.17RR64 pKa = 11.84GPEE67 pKa = 3.36HH68 pKa = 7.14ARR70 pKa = 11.84RR71 pKa = 11.84CNRR74 pKa = 11.84AAAGRR79 pKa = 11.84KK80 pKa = 8.41AAANRR85 pKa = 11.84RR86 pKa = 11.84ANTAPHH92 pKa = 6.22TDD94 pKa = 2.88ATRR97 pKa = 11.84RR98 pKa = 11.84WW99 pKa = 3.47

Molecular weight: 11.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

14281

47

1047

213.1

23.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.429 ± 0.573

1.134 ± 0.155

6.631 ± 0.197

5.609 ± 0.361

2.829 ± 0.193

8.879 ± 0.437

2.031 ± 0.184

4.481 ± 0.19

2.871 ± 0.212

7.822 ± 0.349

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.038 ± 0.148

3.081 ± 0.231

6.253 ± 0.247

3.942 ± 0.241

6.883 ± 0.41

5.245 ± 0.214

6.799 ± 0.288

6.897 ± 0.269

1.891 ± 0.15

2.255 ± 0.148
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski