Amino acid dipepetide frequency for Novosphingobium sp. PhB165

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.224AlaAla: 18.224 ± 0.153	1.085AlaCys: 1.085 ± 0.029	7.154AlaAsp: 7.154 ± 0.066	7.539AlaGlu: 7.539 ± 0.101	4.293AlaPhe: 4.293 ± 0.061	11.213AlaGly: 11.213 ± 0.129	2.453AlaHis: 2.453 ± 0.045	6.354AlaIle: 6.354 ± 0.068	4.026AlaLys: 4.026 ± 0.063	13.613AlaLeu: 13.613 ± 0.124	3.868AlaMet: 3.868 ± 0.052	3.239AlaAsn: 3.239 ± 0.056	6.458AlaPro: 6.458 ± 0.081	4.693AlaGln: 4.693 ± 0.064	9.304AlaArg: 9.304 ± 0.11	7.102AlaSer: 7.102 ± 0.079	6.386AlaThr: 6.386 ± 0.093	8.682AlaVal: 8.682 ± 0.084	1.702AlaTrp: 1.702 ± 0.032	2.595AlaTyr: 2.595 ± 0.046	0.0AlaXaa: 0.0 ± 0.0
Cys	1.04CysAla: 1.04 ± 0.03	0.098CysCys: 0.098 ± 0.008	0.514CysAsp: 0.514 ± 0.019	0.469CysGlu: 0.469 ± 0.018	0.289CysPhe: 0.289 ± 0.013	0.886CysGly: 0.886 ± 0.023	0.236CysHis: 0.236 ± 0.016	0.352CysIle: 0.352 ± 0.014	0.188CysLys: 0.188 ± 0.011	0.732CysLeu: 0.732 ± 0.021	0.175CysMet: 0.175 ± 0.011	0.239CysAsn: 0.239 ± 0.011	0.453CysPro: 0.453 ± 0.018	0.217CysGln: 0.217 ± 0.011	0.579CysArg: 0.579 ± 0.017	0.455CysSer: 0.455 ± 0.017	0.445CysThr: 0.445 ± 0.015	0.54CysVal: 0.54 ± 0.021	0.131CysTrp: 0.131 ± 0.009	0.189CysTyr: 0.189 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	7.254AspAla: 7.254 ± 0.066	0.517AspCys: 0.517 ± 0.018	3.13AspAsp: 3.13 ± 0.044	3.282AspGlu: 3.282 ± 0.055	2.241AspPhe: 2.241 ± 0.04	5.628AspGly: 5.628 ± 0.073	1.314AspHis: 1.314 ± 0.032	2.717AspIle: 2.717 ± 0.041	1.731AspLys: 1.731 ± 0.03	5.746AspLeu: 5.746 ± 0.055	1.364AspMet: 1.364 ± 0.03	1.491AspAsn: 1.491 ± 0.035	3.789AspPro: 3.789 ± 0.052	1.679AspGln: 1.679 ± 0.034	4.286AspArg: 4.286 ± 0.055	2.52AspSer: 2.52 ± 0.05	2.633AspThr: 2.633 ± 0.046	4.111AspVal: 4.111 ± 0.05	1.155AspTrp: 1.155 ± 0.024	1.633AspTyr: 1.633 ± 0.033	0.0AspXaa: 0.0 ± 0.0
Glu	7.7GluAla: 7.7 ± 0.101	0.37GluCys: 0.37 ± 0.017	2.907GluAsp: 2.907 ± 0.057	2.874GluGlu: 2.874 ± 0.056	1.643GluPhe: 1.643 ± 0.033	4.631GluGly: 4.631 ± 0.062	1.273GluHis: 1.273 ± 0.031	2.898GluIle: 2.898 ± 0.05	1.882GluLys: 1.882 ± 0.043	5.07GluLeu: 5.07 ± 0.065	1.391GluMet: 1.391 ± 0.03	1.461GluAsn: 1.461 ± 0.031	2.569GluPro: 2.569 ± 0.043	2.065GluGln: 2.065 ± 0.039	4.57GluArg: 4.57 ± 0.07	2.342GluSer: 2.342 ± 0.036	3.2GluThr: 3.2 ± 0.041	3.775GluVal: 3.775 ± 0.058	0.845GluTrp: 0.845 ± 0.023	1.0GluTyr: 1.0 ± 0.024	0.0GluXaa: 0.0 ± 0.0
Phe	4.934PheAla: 4.934 ± 0.064	0.352PheCys: 0.352 ± 0.015	2.688PheAsp: 2.688 ± 0.039	2.038PheGlu: 2.038 ± 0.037	1.199PhePhe: 1.199 ± 0.033	3.741PheGly: 3.741 ± 0.05	0.825PheHis: 0.825 ± 0.022	1.323PheIle: 1.323 ± 0.029	0.81PheLys: 0.81 ± 0.021	2.96PheLeu: 2.96 ± 0.046	0.744PheMet: 0.744 ± 0.02	1.066PheAsn: 1.066 ± 0.03	1.522PhePro: 1.522 ± 0.035	0.898PheGln: 0.898 ± 0.023	2.127PheArg: 2.127 ± 0.036	2.154PheSer: 2.154 ± 0.038	2.158PheThr: 2.158 ± 0.039	2.661PheVal: 2.661 ± 0.038	0.563PheTrp: 0.563 ± 0.018	0.9PheTyr: 0.9 ± 0.022	0.0PheXaa: 0.0 ± 0.0
Gly	9.973GlyAla: 9.973 ± 0.146	0.835GlyCys: 0.835 ± 0.023	4.78GlyAsp: 4.78 ± 0.077	4.943GlyGlu: 4.943 ± 0.061	3.642GlyPhe: 3.642 ± 0.054	8.603GlyGly: 8.603 ± 0.407	1.925GlyHis: 1.925 ± 0.036	4.425GlyIle: 4.425 ± 0.05	3.514GlyLys: 3.514 ± 0.06	8.738GlyLeu: 8.738 ± 0.084	2.414GlyMet: 2.414 ± 0.043	2.676GlyAsn: 2.676 ± 0.084	3.841GlyPro: 3.841 ± 0.052	3.066GlyGln: 3.066 ± 0.044	6.061GlyArg: 6.061 ± 0.071	5.368GlySer: 5.368 ± 0.1	5.615GlyThr: 5.615 ± 0.129	6.246GlyVal: 6.246 ± 0.074	1.635GlyTrp: 1.635 ± 0.036	2.435GlyTyr: 2.435 ± 0.045	0.0GlyXaa: 0.0 ± 0.0
His	2.585HisAla: 2.585 ± 0.042	0.231HisCys: 0.231 ± 0.011	1.236HisAsp: 1.236 ± 0.026	1.084HisGlu: 1.084 ± 0.028	0.945HisPhe: 0.945 ± 0.023	2.041HisGly: 2.041 ± 0.04	0.569HisHis: 0.569 ± 0.021	0.844HisIle: 0.844 ± 0.025	0.483HisLys: 0.483 ± 0.019	1.896HisLeu: 1.896 ± 0.036	0.516HisMet: 0.516 ± 0.018	0.491HisAsn: 0.491 ± 0.016	1.357HisPro: 1.357 ± 0.032	0.576HisGln: 0.576 ± 0.021	1.443HisArg: 1.443 ± 0.032	1.034HisSer: 1.034 ± 0.024	0.809HisThr: 0.809 ± 0.023	1.588HisVal: 1.588 ± 0.03	0.393HisTrp: 0.393 ± 0.017	0.601HisTyr: 0.601 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	7.401IleAla: 7.401 ± 0.074	0.447IleCys: 0.447 ± 0.017	3.754IleAsp: 3.754 ± 0.041	3.321IleGlu: 3.321 ± 0.05	1.4IlePhe: 1.4 ± 0.031	4.882IleGly: 4.882 ± 0.059	0.905IleHis: 0.905 ± 0.021	1.561IleIle: 1.561 ± 0.036	1.16IleLys: 1.16 ± 0.03	3.597IleLeu: 3.597 ± 0.051	0.81IleMet: 0.81 ± 0.021	1.253IleAsn: 1.253 ± 0.031	2.292IlePro: 2.292 ± 0.04	1.162IleGln: 1.162 ± 0.029	3.013IleArg: 3.013 ± 0.039	2.688IleSer: 2.688 ± 0.041	2.536IleThr: 2.536 ± 0.047	3.79IleVal: 3.79 ± 0.047	0.582IleTrp: 0.582 ± 0.02	1.04IleTyr: 1.04 ± 0.026	0.0IleXaa: 0.0 ± 0.0
Lys	4.102LysAla: 4.102 ± 0.068	0.156LysCys: 0.156 ± 0.011	1.56LysAsp: 1.56 ± 0.038	1.233LysGlu: 1.233 ± 0.033	0.91LysPhe: 0.91 ± 0.025	2.645LysGly: 2.645 ± 0.041	0.512LysHis: 0.512 ± 0.016	1.41LysIle: 1.41 ± 0.028	0.898LysLys: 0.898 ± 0.029	3.045LysLeu: 3.045 ± 0.047	0.711LysMet: 0.711 ± 0.023	0.732LysAsn: 0.732 ± 0.024	1.973LysPro: 1.973 ± 0.046	0.923LysGln: 0.923 ± 0.027	2.022LysArg: 2.022 ± 0.039	1.639LysSer: 1.639 ± 0.034	1.615LysThr: 1.615 ± 0.032	2.341LysVal: 2.341 ± 0.038	0.404LysTrp: 0.404 ± 0.015	0.579LysTyr: 0.579 ± 0.018	0.0LysXaa: 0.0 ± 0.0
Leu	14.011LeuAla: 14.011 ± 0.13	0.854LeuCys: 0.854 ± 0.026	6.087LeuAsp: 6.087 ± 0.061	4.97LeuGlu: 4.97 ± 0.072	3.363LeuPhe: 3.363 ± 0.052	8.559LeuGly: 8.559 ± 0.092	1.857LeuHis: 1.857 ± 0.032	4.368LeuIle: 4.368 ± 0.06	2.808LeuLys: 2.808 ± 0.043	9.138LeuLeu: 9.138 ± 0.119	2.103LeuMet: 2.103 ± 0.039	2.35LeuAsn: 2.35 ± 0.04	5.698LeuPro: 5.698 ± 0.062	2.523LeuGln: 2.523 ± 0.038	6.668LeuArg: 6.668 ± 0.069	6.242LeuSer: 6.242 ± 0.056	5.724LeuThr: 5.724 ± 0.07	7.358LeuVal: 7.358 ± 0.092	1.209LeuTrp: 1.209 ± 0.034	1.896LeuTyr: 1.896 ± 0.034	0.0LeuXaa: 0.0 ± 0.0
Met	3.35MetAla: 3.35 ± 0.048	0.159MetCys: 0.159 ± 0.009	1.156MetAsp: 1.156 ± 0.026	1.099MetGlu: 1.099 ± 0.027	0.708MetPhe: 0.708 ± 0.022	1.891MetGly: 1.891 ± 0.039	0.448MetHis: 0.448 ± 0.018	1.282MetIle: 1.282 ± 0.028	0.847MetLys: 0.847 ± 0.025	2.537MetLeu: 2.537 ± 0.037	0.621MetMet: 0.621 ± 0.022	0.722MetAsn: 0.722 ± 0.022	1.512MetPro: 1.512 ± 0.032	0.793MetGln: 0.793 ± 0.021	1.781MetArg: 1.781 ± 0.035	1.487MetSer: 1.487 ± 0.028	1.777MetThr: 1.777 ± 0.031	1.671MetVal: 1.671 ± 0.033	0.21MetTrp: 0.21 ± 0.012	0.266MetTyr: 0.266 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	3.405AsnAla: 3.405 ± 0.055	0.244AsnCys: 0.244 ± 0.012	1.437AsnAsp: 1.437 ± 0.03	1.133AsnGlu: 1.133 ± 0.024	1.027AsnPhe: 1.027 ± 0.029	2.764AsnGly: 2.764 ± 0.073	0.529AsnHis: 0.529 ± 0.018	1.197AsnIle: 1.197 ± 0.029	0.62AsnLys: 0.62 ± 0.019	2.638AsnLeu: 2.638 ± 0.04	0.55AsnMet: 0.55 ± 0.016	0.744AsnAsn: 0.744 ± 0.026	1.857AsnPro: 1.857 ± 0.037	0.773AsnGln: 0.773 ± 0.023	1.83AsnArg: 1.83 ± 0.033	1.433AsnSer: 1.433 ± 0.032	1.324AsnThr: 1.324 ± 0.04	2.076AsnVal: 2.076 ± 0.039	0.464AsnTrp: 0.464 ± 0.017	0.791AsnTyr: 0.791 ± 0.045	0.0AsnXaa: 0.0 ± 0.0
Pro	7.088ProAla: 7.088 ± 0.074	0.348ProCys: 0.348 ± 0.014	3.647ProAsp: 3.647 ± 0.055	3.761ProGlu: 3.761 ± 0.059	1.928ProPhe: 1.928 ± 0.041	4.955ProGly: 4.955 ± 0.062	1.098ProHis: 1.098 ± 0.025	2.334ProIle: 2.334 ± 0.039	1.493ProLys: 1.493 ± 0.039	4.97ProLeu: 4.97 ± 0.061	1.268ProMet: 1.268 ± 0.03	1.338ProAsn: 1.338 ± 0.029	2.632ProPro: 2.632 ± 0.061	1.845ProGln: 1.845 ± 0.032	3.122ProArg: 3.122 ± 0.053	2.987ProSer: 2.987 ± 0.044	2.668ProThr: 2.668 ± 0.058	4.424ProVal: 4.424 ± 0.058	0.71ProTrp: 0.71 ± 0.021	1.131ProTyr: 1.131 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	4.233GlnAla: 4.233 ± 0.051	0.233GlnCys: 0.233 ± 0.012	1.544GlnAsp: 1.544 ± 0.029	1.282GlnGlu: 1.282 ± 0.026	1.192GlnPhe: 1.192 ± 0.028	2.652GlnGly: 2.652 ± 0.043	0.647GlnHis: 0.647 ± 0.023	1.73GlnIle: 1.73 ± 0.03	0.929GlnLys: 0.929 ± 0.025	3.039GlnLeu: 3.039 ± 0.05	0.822GlnMet: 0.822 ± 0.022	0.798GlnAsn: 0.798 ± 0.026	1.875GlnPro: 1.875 ± 0.038	1.243GlnGln: 1.243 ± 0.032	2.223GlnArg: 2.223 ± 0.043	1.902GlnSer: 1.902 ± 0.056	1.724GlnThr: 1.724 ± 0.04	2.502GlnVal: 2.502 ± 0.041	0.48GlnTrp: 0.48 ± 0.015	0.676GlnTyr: 0.676 ± 0.025	0.0GlnXaa: 0.0 ± 0.0
Arg	7.981ArgAla: 7.981 ± 0.092	0.49ArgCys: 0.49 ± 0.017	3.937ArgAsp: 3.937 ± 0.058	4.148ArgGlu: 4.148 ± 0.063	3.001ArgPhe: 3.001 ± 0.047	4.917ArgGly: 4.917 ± 0.06	1.711ArgHis: 1.711 ± 0.035	3.92ArgIle: 3.92 ± 0.058	2.21ArgLys: 2.21 ± 0.042	7.511ArgLeu: 7.511 ± 0.092	1.885ArgMet: 1.885 ± 0.034	1.853ArgAsn: 1.853 ± 0.034	3.508ArgPro: 3.508 ± 0.066	2.447ArgGln: 2.447 ± 0.046	5.345ArgArg: 5.345 ± 0.076	3.566ArgSer: 3.566 ± 0.049	3.432ArgThr: 3.432 ± 0.048	4.625ArgVal: 4.625 ± 0.059	1.169ArgTrp: 1.169 ± 0.029	1.828ArgTyr: 1.828 ± 0.035	0.0ArgXaa: 0.0 ± 0.0
Ser	6.95SerAla: 6.95 ± 0.083	0.44SerCys: 0.44 ± 0.017	3.184SerAsp: 3.184 ± 0.049	2.722SerGlu: 2.722 ± 0.045	2.094SerPhe: 2.094 ± 0.033	6.183SerGly: 6.183 ± 0.114	1.093SerHis: 1.093 ± 0.027	2.699SerIle: 2.699 ± 0.052	1.453SerLys: 1.453 ± 0.033	5.597SerLeu: 5.597 ± 0.065	1.276SerMet: 1.276 ± 0.027	1.583SerAsn: 1.583 ± 0.039	3.029SerPro: 3.029 ± 0.047	1.764SerGln: 1.764 ± 0.037	3.596SerArg: 3.596 ± 0.051	3.1SerSer: 3.1 ± 0.063	2.966SerThr: 2.966 ± 0.059	3.866SerVal: 3.866 ± 0.08	0.827SerTrp: 0.827 ± 0.022	1.467SerTyr: 1.467 ± 0.036	0.0SerXaa: 0.0 ± 0.0
Thr	6.615ThrAla: 6.615 ± 0.1	0.416ThrCys: 0.416 ± 0.017	2.93ThrAsp: 2.93 ± 0.053	2.394ThrGlu: 2.394 ± 0.038	1.997ThrPhe: 1.997 ± 0.036	5.7ThrGly: 5.7 ± 0.091	0.968ThrHis: 0.968 ± 0.024	2.909ThrIle: 2.909 ± 0.059	1.266ThrLys: 1.266 ± 0.036	5.891ThrLeu: 5.891 ± 0.094	1.3ThrMet: 1.3 ± 0.03	1.407ThrAsn: 1.407 ± 0.036	3.427ThrPro: 3.427 ± 0.044	1.565ThrGln: 1.565 ± 0.034	3.444ThrArg: 3.444 ± 0.046	3.039ThrSer: 3.039 ± 0.056	2.969ThrThr: 2.969 ± 0.072	4.6ThrVal: 4.6 ± 0.076	0.701ThrTrp: 0.701 ± 0.021	1.367ThrTyr: 1.367 ± 0.038	0.0ThrXaa: 0.0 ± 0.0
Val	9.019ValAla: 9.019 ± 0.082	0.592ValCys: 0.592 ± 0.019	4.222ValAsp: 4.222 ± 0.051	4.366ValGlu: 4.366 ± 0.061	2.285ValPhe: 2.285 ± 0.039	5.625ValGly: 5.625 ± 0.081	1.433ValHis: 1.433 ± 0.033	3.742ValIle: 3.742 ± 0.05	2.005ValLys: 2.005 ± 0.04	7.162ValLeu: 7.162 ± 0.082	1.717ValMet: 1.717 ± 0.035	2.146ValAsn: 2.146 ± 0.044	4.081ValPro: 4.081 ± 0.052	2.097ValGln: 2.097 ± 0.036	4.92ValArg: 4.92 ± 0.061	4.556ValSer: 4.556 ± 0.068	4.863ValThr: 4.863 ± 0.08	5.498ValVal: 5.498 ± 0.061	0.943ValTrp: 0.943 ± 0.026	1.442ValTyr: 1.442 ± 0.03	0.0ValXaa: 0.0 ± 0.0
Trp	1.372TrpAla: 1.372 ± 0.031	0.135TrpCys: 0.135 ± 0.009	0.783TrpAsp: 0.783 ± 0.025	0.616TrpGlu: 0.616 ± 0.022	0.592TrpPhe: 0.592 ± 0.019	1.028TrpGly: 1.028 ± 0.026	0.399TrpHis: 0.399 ± 0.017	0.701TrpIle: 0.701 ± 0.02	0.506TrpLys: 0.506 ± 0.017	1.787TrpLeu: 1.787 ± 0.034	0.368TrpMet: 0.368 ± 0.017	0.533TrpAsn: 0.533 ± 0.021	0.757TrpPro: 0.757 ± 0.021	0.659TrpGln: 0.659 ± 0.016	1.308TrpArg: 1.308 ± 0.029	0.968TrpSer: 0.968 ± 0.027	0.844TrpThr: 0.844 ± 0.025	0.826TrpVal: 0.826 ± 0.023	0.263TrpTrp: 0.263 ± 0.014	0.316TrpTyr: 0.316 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.747TyrAla: 2.747 ± 0.044	0.223TyrCys: 0.223 ± 0.012	1.57TyrAsp: 1.57 ± 0.046	1.194TyrGlu: 1.194 ± 0.028	0.916TyrPhe: 0.916 ± 0.024	2.193TyrGly: 2.193 ± 0.044	0.526TyrHis: 0.526 ± 0.019	0.826TyrIle: 0.826 ± 0.022	0.58TyrLys: 0.58 ± 0.021	2.122TyrLeu: 2.122 ± 0.035	0.415TyrMet: 0.415 ± 0.016	0.713TyrAsn: 0.713 ± 0.025	1.117TyrPro: 1.117 ± 0.024	0.739TyrGln: 0.739 ± 0.024	1.843TyrArg: 1.843 ± 0.038	1.308TyrSer: 1.308 ± 0.033	1.217TyrThr: 1.217 ± 0.033	1.531TyrVal: 1.531 ± 0.03	0.372TyrTrp: 0.372 ± 0.013	0.666TyrTyr: 0.666 ± 0.024	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5096 proteins (1697015 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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