Streptococcus satellite phage Javan217

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZGE6|A0A4D5ZGE6_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan217 OX=2558574 GN=JavanS217_0004 PE=4 SV=1
MM1 pKa = 7.57LVRR4 pKa = 11.84LEE6 pKa = 4.24FSDD9 pKa = 4.86GNTLTRR15 pKa = 11.84MVSKK19 pKa = 9.15HH20 pKa = 5.01TLDD23 pKa = 4.03MILARR28 pKa = 11.84PYY30 pKa = 10.92SEE32 pKa = 3.92TWLHH36 pKa = 6.67FNGEE40 pKa = 4.28KK41 pKa = 10.04IAVLEE46 pKa = 4.01NVEE49 pKa = 4.1VLGNVDD55 pKa = 4.92DD56 pKa = 5.97FDD58 pKa = 5.52NEE60 pKa = 4.21VNKK63 pKa = 10.09TIAYY67 pKa = 9.79NEE69 pKa = 4.4LEE71 pKa = 4.3NEE73 pKa = 4.27VQEE76 pKa = 4.56NEE78 pKa = 3.94

Molecular weight: 9.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZGP8|A0A4D5ZGP8_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan217 OX=2558574 GN=JavanS217_0011 PE=4 SV=1
MM1 pKa = 8.09AEE3 pKa = 3.91IKK5 pKa = 10.55KK6 pKa = 8.29ITKK9 pKa = 10.26KK10 pKa = 10.74NGTTVYY16 pKa = 10.04RR17 pKa = 11.84QQIYY21 pKa = 10.8LGTDD25 pKa = 3.14CMTGKK30 pKa = 9.72QVYY33 pKa = 8.65TSISAPTKK41 pKa = 10.55KK42 pKa = 9.92EE43 pKa = 3.83LKK45 pKa = 9.55QKK47 pKa = 10.85RR48 pKa = 11.84EE49 pKa = 3.75FKK51 pKa = 10.77INEE54 pKa = 4.18FKK56 pKa = 11.23DD57 pKa = 3.31NGYY60 pKa = 7.94TRR62 pKa = 11.84YY63 pKa = 10.57KK64 pKa = 10.47SVTVKK69 pKa = 10.57NYY71 pKa = 10.67RR72 pKa = 11.84EE73 pKa = 4.16LSEE76 pKa = 4.8LWLEE80 pKa = 4.02NHH82 pKa = 6.29KK83 pKa = 11.28LEE85 pKa = 5.31VKK87 pKa = 9.27PQSYY91 pKa = 10.89KK92 pKa = 9.75NTIIKK97 pKa = 9.6LNKK100 pKa = 9.2YY101 pKa = 8.6LLPVFGDD108 pKa = 3.35MKK110 pKa = 10.73VEE112 pKa = 4.57KK113 pKa = 9.09ITLPMVQNFVNKK125 pKa = 10.19LVGSEE130 pKa = 3.89LTSYY134 pKa = 10.87KK135 pKa = 10.58VVISINKK142 pKa = 9.65RR143 pKa = 11.84ILKK146 pKa = 9.77YY147 pKa = 10.46AVNLQIINVNPADD160 pKa = 3.76NVIVPKK166 pKa = 10.52VKK168 pKa = 10.1KK169 pKa = 10.12SKK171 pKa = 10.2KK172 pKa = 9.04EE173 pKa = 3.93KK174 pKa = 10.31VKK176 pKa = 10.98HH177 pKa = 6.29FDD179 pKa = 4.08DD180 pKa = 4.37SQLKK184 pKa = 8.91QFKK187 pKa = 10.52DD188 pKa = 3.78YY189 pKa = 10.76MNSLSDD195 pKa = 3.42TFRR198 pKa = 11.84NCYY201 pKa = 10.17YY202 pKa = 9.33KK203 pKa = 10.01TLYY206 pKa = 8.72YY207 pKa = 10.26TLLASGLRR215 pKa = 11.84IGEE218 pKa = 4.49AIALEE223 pKa = 4.01WSDD226 pKa = 5.15IYY228 pKa = 11.51LEE230 pKa = 4.95DD231 pKa = 5.18LDD233 pKa = 4.92NGYY236 pKa = 10.04IDD238 pKa = 3.62VNKK241 pKa = 10.13TYY243 pKa = 10.68VEE245 pKa = 4.16KK246 pKa = 10.8EE247 pKa = 3.85KK248 pKa = 11.03GVNDD252 pKa = 4.14PKK254 pKa = 11.08SEE256 pKa = 3.94AGKK259 pKa = 10.28RR260 pKa = 11.84RR261 pKa = 11.84VPIDD265 pKa = 3.41RR266 pKa = 11.84NTVLMLRR273 pKa = 11.84LYY275 pKa = 10.42KK276 pKa = 10.33ARR278 pKa = 11.84QRR280 pKa = 11.84QCFMLNGYY288 pKa = 7.03GAKK291 pKa = 8.14MTEE294 pKa = 4.21HH295 pKa = 6.15VFSNGFSAYY304 pKa = 6.65PTTVGLQYY312 pKa = 11.56VLTQHH317 pKa = 6.82LQQAGLPRR325 pKa = 11.84FTFHH329 pKa = 7.9AFRR332 pKa = 11.84HH333 pKa = 4.69THH335 pKa = 6.92ASLLLNAGIGYY346 pKa = 9.74KK347 pKa = 9.96EE348 pKa = 3.9LQHH351 pKa = 6.77RR352 pKa = 11.84LGHH355 pKa = 5.56STLAMTMDD363 pKa = 4.54TYY365 pKa = 11.85SHH367 pKa = 7.05LSKK370 pKa = 10.7EE371 pKa = 4.25KK372 pKa = 10.83EE373 pKa = 3.53KK374 pKa = 11.22DD375 pKa = 3.16AVNFFEE381 pKa = 5.73KK382 pKa = 10.83AMANLL387 pKa = 3.66

Molecular weight: 44.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16

0

16

2255

37

476

140.9

16.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.922 ± 0.49

0.443 ± 0.108

6.519 ± 0.661

8.337 ± 0.739

3.725 ± 0.383

4.346 ± 0.36

1.552 ± 0.284

6.696 ± 0.559

10.067 ± 0.437

10.51 ± 0.925

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.572 ± 0.367

6.519 ± 0.446

2.217 ± 0.256

3.681 ± 0.289

4.435 ± 0.511

5.011 ± 0.328

6.164 ± 0.356

6.519 ± 0.494

0.843 ± 0.15

4.922 ± 0.554
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski