Psychromonas sp. CNPT3

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Psychromonadaceae; Psychromonas; unclassified Psychromonas

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2578 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4UP76|M4UP76_9GAMM PTS system N-acetylgalactosamine-specific transporter subunit IID OS=Psychromonas sp. CNPT3 OX=314282 GN=PCNPT3_11895 PE=4 SV=1
MM1 pKa = 7.68KK2 pKa = 9.43KK3 pKa = 9.06TILAMAITGLFAATAAQAATVYY25 pKa = 10.66DD26 pKa = 3.96ADD28 pKa = 4.42GVTLKK33 pKa = 11.18LNGDD37 pKa = 3.55VKK39 pKa = 10.83INYY42 pKa = 9.49GSDD45 pKa = 2.96VRR47 pKa = 11.84KK48 pKa = 10.17FNDD51 pKa = 3.04NKK53 pKa = 10.26VDD55 pKa = 3.6SNYY58 pKa = 9.86IEE60 pKa = 5.87LDD62 pKa = 3.48DD63 pKa = 5.59ADD65 pKa = 4.43FSFALGYY72 pKa = 10.51EE73 pKa = 3.9IGNGIEE79 pKa = 4.61LGATMEE85 pKa = 5.08ISGEE89 pKa = 3.99DD90 pKa = 3.64DD91 pKa = 4.43NIALSDD97 pKa = 3.7TFLSVAGDD105 pKa = 4.15FGTVTVGKK113 pKa = 10.08QPLIFDD119 pKa = 4.1DD120 pKa = 4.37AGIGEE125 pKa = 4.35DD126 pKa = 3.97FKK128 pKa = 11.62FGFDD132 pKa = 3.26TYY134 pKa = 11.89ANTTDD139 pKa = 4.02SGEE142 pKa = 3.92QVIKK146 pKa = 11.27YY147 pKa = 10.14KK148 pKa = 10.98GDD150 pKa = 3.05WDD152 pKa = 3.66TFYY155 pKa = 11.47AGVAYY160 pKa = 9.27MLNGDD165 pKa = 3.79ATSNGDD171 pKa = 3.21GTTGEE176 pKa = 4.14DD177 pKa = 4.87DD178 pKa = 4.21KK179 pKa = 11.91NQVDD183 pKa = 3.65ANFGVRR189 pKa = 11.84FAGIDD194 pKa = 3.26AAVYY198 pKa = 10.68YY199 pKa = 9.79STGTNNLKK207 pKa = 10.05QDD209 pKa = 3.33NNAYY213 pKa = 9.63ILQAMYY219 pKa = 10.56NADD222 pKa = 3.69AFKK225 pKa = 11.14VGAFYY230 pKa = 11.22SNEE233 pKa = 4.05TIEE236 pKa = 4.34NSNGSDD242 pKa = 3.69LSDD245 pKa = 3.73LNHH248 pKa = 6.41YY249 pKa = 8.88GVSGNYY255 pKa = 10.35YY256 pKa = 8.33MDD258 pKa = 3.05AWNFGLGWGAEE269 pKa = 3.97KK270 pKa = 11.12DD271 pKa = 3.64NLTSNNDD278 pKa = 3.39RR279 pKa = 11.84NDD281 pKa = 3.07YY282 pKa = 8.53YY283 pKa = 11.88ANVAYY288 pKa = 10.27AFTSNIQSYY297 pKa = 9.65VEE299 pKa = 3.9VGYY302 pKa = 10.91SDD304 pKa = 4.83EE305 pKa = 5.21DD306 pKa = 4.04DD307 pKa = 4.68SEE309 pKa = 4.34VGYY312 pKa = 10.56VVGMEE317 pKa = 4.24VVFF320 pKa = 4.62

Molecular weight: 34.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4UI09|M4UI09_9GAMM Met regulon regulatory protein MetJ OS=Psychromonas sp. CNPT3 OX=314282 GN=PCNPT3_00390 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNIKK11 pKa = 10.11RR12 pKa = 11.84KK13 pKa = 8.95RR14 pKa = 11.84SHH16 pKa = 6.27GFRR19 pKa = 11.84TRR21 pKa = 11.84MATKK25 pKa = 10.2NGRR28 pKa = 11.84NVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.5GRR39 pKa = 11.84ASLSAA44 pKa = 3.83

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2578

0

2578

852767

28

3350

330.8

36.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.087 ± 0.052

1.215 ± 0.02

5.357 ± 0.051

5.601 ± 0.062

4.398 ± 0.044

6.179 ± 0.047

2.243 ± 0.025

7.587 ± 0.046

6.503 ± 0.047

11.035 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.567 ± 0.027

4.682 ± 0.041

3.427 ± 0.025

4.562 ± 0.04

4.007 ± 0.037

6.909 ± 0.042

5.182 ± 0.047

6.259 ± 0.04

1.047 ± 0.019

3.154 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski