Amino acid dipepetide frequency for Paenibacillus sp. IHBB 10380

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.755AlaAla: 5.755 ± 0.096	0.655AlaCys: 0.655 ± 0.023	3.503AlaAsp: 3.503 ± 0.056	4.533AlaGlu: 4.533 ± 0.062	2.926AlaPhe: 2.926 ± 0.05	5.277AlaGly: 5.277 ± 0.085	1.238AlaHis: 1.238 ± 0.034	5.296AlaIle: 5.296 ± 0.07	3.942AlaLys: 3.942 ± 0.06	7.259AlaLeu: 7.259 ± 0.082	2.067AlaMet: 2.067 ± 0.038	2.613AlaAsn: 2.613 ± 0.046	2.102AlaPro: 2.102 ± 0.042	2.393AlaGln: 2.393 ± 0.043	2.819AlaArg: 2.819 ± 0.059	4.351AlaSer: 4.351 ± 0.056	3.627AlaThr: 3.627 ± 0.06	5.367AlaVal: 5.367 ± 0.065	0.724AlaTrp: 0.724 ± 0.024	2.393AlaTyr: 2.393 ± 0.058	0.0AlaXaa: 0.0 ± 0.0
Cys	0.486CysAla: 0.486 ± 0.02	0.106CysCys: 0.106 ± 0.009	0.416CysAsp: 0.416 ± 0.016	0.427CysGlu: 0.427 ± 0.019	0.321CysPhe: 0.321 ± 0.017	0.73CysGly: 0.73 ± 0.023	0.205CysHis: 0.205 ± 0.013	0.613CysIle: 0.613 ± 0.021	0.392CysLys: 0.392 ± 0.016	0.797CysLeu: 0.797 ± 0.027	0.241CysMet: 0.241 ± 0.014	0.337CysAsn: 0.337 ± 0.015	0.336CysPro: 0.336 ± 0.018	0.268CysGln: 0.268 ± 0.015	0.375CysArg: 0.375 ± 0.018	0.572CysSer: 0.572 ± 0.022	0.408CysThr: 0.408 ± 0.018	0.459CysVal: 0.459 ± 0.018	0.084CysTrp: 0.084 ± 0.007	0.295CysTyr: 0.295 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	3.275AspAla: 3.275 ± 0.053	0.377AspCys: 0.377 ± 0.016	2.516AspAsp: 2.516 ± 0.049	3.817AspGlu: 3.817 ± 0.059	2.159AspPhe: 2.159 ± 0.041	3.487AspGly: 3.487 ± 0.055	1.27AspHis: 1.27 ± 0.032	4.283AspIle: 4.283 ± 0.065	3.128AspLys: 3.128 ± 0.056	5.125AspLeu: 5.125 ± 0.063	1.5AspMet: 1.5 ± 0.035	2.101AspAsn: 2.101 ± 0.045	2.048AspPro: 2.048 ± 0.043	2.08AspGln: 2.08 ± 0.041	2.447AspArg: 2.447 ± 0.047	3.034AspSer: 3.034 ± 0.05	2.682AspThr: 2.682 ± 0.043	3.719AspVal: 3.719 ± 0.048	0.687AspTrp: 0.687 ± 0.021	2.116AspTyr: 2.116 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	5.185GluAla: 5.185 ± 0.077	0.424GluCys: 0.424 ± 0.018	3.514GluAsp: 3.514 ± 0.055	5.612GluGlu: 5.612 ± 0.108	2.317GluPhe: 2.317 ± 0.037	4.432GluGly: 4.432 ± 0.079	1.634GluHis: 1.634 ± 0.039	5.139GluIle: 5.139 ± 0.069	4.382GluLys: 4.382 ± 0.077	7.161GluLeu: 7.161 ± 0.082	2.211GluMet: 2.211 ± 0.044	2.757GluAsn: 2.757 ± 0.045	2.025GluPro: 2.025 ± 0.042	3.806GluGln: 3.806 ± 0.062	3.571GluArg: 3.571 ± 0.067	3.773GluSer: 3.773 ± 0.046	3.168GluThr: 3.168 ± 0.054	4.877GluVal: 4.877 ± 0.071	0.871GluTrp: 0.871 ± 0.024	2.21GluTyr: 2.21 ± 0.044	0.0GluXaa: 0.0 ± 0.0
Phe	2.861PheAla: 2.861 ± 0.052	0.384PheCys: 0.384 ± 0.015	2.364PheAsp: 2.364 ± 0.041	2.532PheGlu: 2.532 ± 0.041	1.879PhePhe: 1.879 ± 0.048	3.05PheGly: 3.05 ± 0.05	0.923PheHis: 0.923 ± 0.026	3.475PheIle: 3.475 ± 0.05	2.122PheLys: 2.122 ± 0.042	3.873PheLeu: 3.873 ± 0.066	1.268PheMet: 1.268 ± 0.035	1.887PheAsn: 1.887 ± 0.043	1.522PhePro: 1.522 ± 0.037	1.488PheGln: 1.488 ± 0.036	1.77PheArg: 1.77 ± 0.034	3.026PheSer: 3.026 ± 0.052	2.527PheThr: 2.527 ± 0.046	2.992PheVal: 2.992 ± 0.051	0.52PheTrp: 0.52 ± 0.02	1.463PheTyr: 1.463 ± 0.039	0.0PheXaa: 0.0 ± 0.0
Gly	4.646GlyAla: 4.646 ± 0.077	0.642GlyCys: 0.642 ± 0.02	3.327GlyAsp: 3.327 ± 0.05	4.418GlyGlu: 4.418 ± 0.091	3.144GlyPhe: 3.144 ± 0.047	4.823GlyGly: 4.823 ± 0.07	1.457GlyHis: 1.457 ± 0.036	6.088GlyIle: 6.088 ± 0.067	4.371GlyLys: 4.371 ± 0.065	6.795GlyLeu: 6.795 ± 0.077	2.311GlyMet: 2.311 ± 0.041	2.844GlyAsn: 2.844 ± 0.05	1.663GlyPro: 1.663 ± 0.041	2.509GlyGln: 2.509 ± 0.042	2.98GlyArg: 2.98 ± 0.045	4.474GlySer: 4.474 ± 0.066	4.001GlyThr: 4.001 ± 0.059	5.026GlyVal: 5.026 ± 0.069	0.913GlyTrp: 0.913 ± 0.027	2.862GlyTyr: 2.862 ± 0.044	0.0GlyXaa: 0.0 ± 0.0
His	1.393HisAla: 1.393 ± 0.035	0.205HisCys: 0.205 ± 0.013	1.073HisAsp: 1.073 ± 0.03	1.367HisGlu: 1.367 ± 0.029	1.016HisPhe: 1.016 ± 0.026	1.457HisGly: 1.457 ± 0.034	0.644HisHis: 0.644 ± 0.026	1.717HisIle: 1.717 ± 0.039	1.044HisLys: 1.044 ± 0.029	2.162HisLeu: 2.162 ± 0.04	0.67HisMet: 0.67 ± 0.021	0.837HisAsn: 0.837 ± 0.026	1.137HisPro: 1.137 ± 0.031	0.845HisGln: 0.845 ± 0.026	1.026HisArg: 1.026 ± 0.029	1.364HisSer: 1.364 ± 0.036	1.146HisThr: 1.146 ± 0.031	1.486HisVal: 1.486 ± 0.035	0.288HisTrp: 0.288 ± 0.014	0.899HisTyr: 0.899 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	5.818IleAla: 5.818 ± 0.07	0.75IleCys: 0.75 ± 0.025	4.062IleAsp: 4.062 ± 0.068	5.112IleGlu: 5.112 ± 0.072	2.9IlePhe: 2.9 ± 0.062	5.643IleGly: 5.643 ± 0.074	1.835IleHis: 1.835 ± 0.04	5.687IleIle: 5.687 ± 0.085	3.845IleLys: 3.845 ± 0.056	7.008IleLeu: 7.008 ± 0.08	2.079IleMet: 2.079 ± 0.043	2.991IleAsn: 2.991 ± 0.056	3.419IlePro: 3.419 ± 0.052	3.12IleGln: 3.12 ± 0.051	3.62IleArg: 3.62 ± 0.054	5.528IleSer: 5.528 ± 0.074	4.373IleThr: 4.373 ± 0.057	5.942IleVal: 5.942 ± 0.075	0.763IleTrp: 0.763 ± 0.027	2.451IleTyr: 2.451 ± 0.049	0.0IleXaa: 0.0 ± 0.0
Lys	3.88LysAla: 3.88 ± 0.058	0.29LysCys: 0.29 ± 0.016	3.289LysAsp: 3.289 ± 0.054	4.95LysGlu: 4.95 ± 0.067	1.853LysPhe: 1.853 ± 0.038	3.85LysGly: 3.85 ± 0.055	1.174LysHis: 1.174 ± 0.036	3.828LysIle: 3.828 ± 0.064	3.959LysLys: 3.959 ± 0.07	5.713LysLeu: 5.713 ± 0.064	1.831LysMet: 1.831 ± 0.039	2.567LysAsn: 2.567 ± 0.051	2.112LysPro: 2.112 ± 0.038	2.666LysGln: 2.666 ± 0.045	2.829LysArg: 2.829 ± 0.052	3.535LysSer: 3.535 ± 0.05	2.964LysThr: 2.964 ± 0.046	4.158LysVal: 4.158 ± 0.06	0.749LysTrp: 0.749 ± 0.025	2.141LysTyr: 2.141 ± 0.046	0.0LysXaa: 0.0 ± 0.0
Leu	6.964LeuAla: 6.964 ± 0.08	0.884LeuCys: 0.884 ± 0.031	5.27LeuAsp: 5.27 ± 0.069	6.51LeuGlu: 6.51 ± 0.088	4.617LeuPhe: 4.617 ± 0.069	6.691LeuGly: 6.691 ± 0.087	2.223LeuHis: 2.223 ± 0.039	7.245LeuIle: 7.245 ± 0.087	5.67LeuLys: 5.67 ± 0.058	10.861LeuLeu: 10.861 ± 0.12	2.724LeuMet: 2.724 ± 0.047	4.374LeuAsn: 4.374 ± 0.059	4.1LeuPro: 4.1 ± 0.062	4.134LeuGln: 4.134 ± 0.06	4.479LeuArg: 4.479 ± 0.06	7.567LeuSer: 7.567 ± 0.084	5.701LeuThr: 5.701 ± 0.067	6.468LeuVal: 6.468 ± 0.069	0.985LeuTrp: 0.985 ± 0.027	3.171LeuTyr: 3.171 ± 0.048	0.0LeuXaa: 0.0 ± 0.0
Met	2.082MetAla: 2.082 ± 0.037	0.172MetCys: 0.172 ± 0.01	1.723MetAsp: 1.723 ± 0.036	1.977MetGlu: 1.977 ± 0.035	1.118MetPhe: 1.118 ± 0.029	1.976MetGly: 1.976 ± 0.038	0.462MetHis: 0.462 ± 0.021	2.339MetIle: 2.339 ± 0.044	2.317MetLys: 2.317 ± 0.044	2.985MetLeu: 2.985 ± 0.048	0.991MetMet: 0.991 ± 0.028	1.783MetAsn: 1.783 ± 0.041	1.053MetPro: 1.053 ± 0.025	1.046MetGln: 1.046 ± 0.029	1.256MetArg: 1.256 ± 0.029	2.033MetSer: 2.033 ± 0.034	1.72MetThr: 1.72 ± 0.035	1.91MetVal: 1.91 ± 0.042	0.227MetTrp: 0.227 ± 0.013	0.835MetTyr: 0.835 ± 0.03	0.0MetXaa: 0.0 ± 0.0
Asn	2.701AsnAla: 2.701 ± 0.045	0.29AsnCys: 0.29 ± 0.015	2.194AsnAsp: 2.194 ± 0.044	2.993AsnGlu: 2.993 ± 0.055	1.541AsnPhe: 1.541 ± 0.03	3.137AsnGly: 3.137 ± 0.048	1.069AsnHis: 1.069 ± 0.03	3.316AsnIle: 3.316 ± 0.056	2.717AsnLys: 2.717 ± 0.052	3.763AsnLeu: 3.763 ± 0.055	1.294AsnMet: 1.294 ± 0.033	2.172AsnAsn: 2.172 ± 0.055	2.09AsnPro: 2.09 ± 0.033	1.769AsnGln: 1.769 ± 0.041	2.022AsnArg: 2.022 ± 0.044	2.538AsnSer: 2.538 ± 0.051	2.289AsnThr: 2.289 ± 0.041	2.893AsnVal: 2.893 ± 0.047	0.51AsnTrp: 0.51 ± 0.016	1.551AsnTyr: 1.551 ± 0.036	0.0AsnXaa: 0.0 ± 0.0
Pro	2.288ProAla: 2.288 ± 0.045	0.237ProCys: 0.237 ± 0.015	2.204ProAsp: 2.204 ± 0.043	3.014ProGlu: 3.014 ± 0.051	1.825ProPhe: 1.825 ± 0.042	2.23ProGly: 2.23 ± 0.043	0.79ProHis: 0.79 ± 0.026	2.834ProIle: 2.834 ± 0.053	1.855ProLys: 1.855 ± 0.035	3.724ProLeu: 3.724 ± 0.054	0.911ProMet: 0.911 ± 0.024	1.651ProAsn: 1.651 ± 0.034	0.996ProPro: 0.996 ± 0.031	1.314ProGln: 1.314 ± 0.033	1.296ProArg: 1.296 ± 0.034	2.497ProSer: 2.497 ± 0.041	2.109ProThr: 2.109 ± 0.035	2.766ProVal: 2.766 ± 0.047	0.46ProTrp: 0.46 ± 0.019	1.44ProTyr: 1.44 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	2.868GlnAla: 2.868 ± 0.048	0.246GlnCys: 0.246 ± 0.015	1.908GlnAsp: 1.908 ± 0.037	3.0GlnGlu: 3.0 ± 0.056	1.659GlnPhe: 1.659 ± 0.041	2.58GlnGly: 2.58 ± 0.039	0.857GlnHis: 0.857 ± 0.026	2.842GlnIle: 2.842 ± 0.05	2.145GlnLys: 2.145 ± 0.043	4.352GlnLeu: 4.352 ± 0.06	1.294GlnMet: 1.294 ± 0.031	1.514GlnAsn: 1.514 ± 0.031	1.314GlnPro: 1.314 ± 0.031	2.111GlnGln: 2.111 ± 0.049	1.881GlnArg: 1.881 ± 0.042	2.545GlnSer: 2.545 ± 0.049	1.971GlnThr: 1.971 ± 0.038	2.752GlnVal: 2.752 ± 0.06	0.506GlnTrp: 0.506 ± 0.019	1.496GlnTyr: 1.496 ± 0.034	0.0GlnXaa: 0.0 ± 0.0
Arg	2.586ArgAla: 2.586 ± 0.043	0.341ArgCys: 0.341 ± 0.016	2.217ArgAsp: 2.217 ± 0.041	3.346ArgGlu: 3.346 ± 0.06	1.898ArgPhe: 1.898 ± 0.039	2.719ArgGly: 2.719 ± 0.05	0.949ArgHis: 0.949 ± 0.027	3.708ArgIle: 3.708 ± 0.059	2.999ArgLys: 2.999 ± 0.054	4.527ArgLeu: 4.527 ± 0.069	1.568ArgMet: 1.568 ± 0.034	2.063ArgAsn: 2.063 ± 0.037	1.381ArgPro: 1.381 ± 0.039	1.798ArgGln: 1.798 ± 0.04	2.293ArgArg: 2.293 ± 0.051	2.939ArgSer: 2.939 ± 0.048	2.398ArgThr: 2.398 ± 0.036	2.948ArgVal: 2.948 ± 0.047	0.547ArgTrp: 0.547 ± 0.022	1.76ArgTyr: 1.76 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	4.188SerAla: 4.188 ± 0.058	0.451SerCys: 0.451 ± 0.019	3.267SerAsp: 3.267 ± 0.046	4.185SerGlu: 4.185 ± 0.054	3.193SerPhe: 3.193 ± 0.051	5.061SerGly: 5.061 ± 0.063	1.395SerHis: 1.395 ± 0.03	5.265SerIle: 5.265 ± 0.07	3.875SerLys: 3.875 ± 0.06	6.895SerLeu: 6.895 ± 0.064	1.989SerMet: 1.989 ± 0.038	2.881SerAsn: 2.881 ± 0.049	2.294SerPro: 2.294 ± 0.047	2.344SerGln: 2.344 ± 0.048	2.885SerArg: 2.885 ± 0.045	4.769SerSer: 4.769 ± 0.075	3.628SerThr: 3.628 ± 0.051	4.612SerVal: 4.612 ± 0.059	0.799SerTrp: 0.799 ± 0.024	2.501SerTyr: 2.501 ± 0.046	0.0SerXaa: 0.0 ± 0.0
Thr	3.982ThrAla: 3.982 ± 0.066	0.367ThrCys: 0.367 ± 0.017	2.722ThrAsp: 2.722 ± 0.05	3.482ThrGlu: 3.482 ± 0.06	2.504ThrPhe: 2.504 ± 0.04	4.211ThrGly: 4.211 ± 0.059	1.121ThrHis: 1.121 ± 0.028	4.312ThrIle: 4.312 ± 0.061	2.842ThrLys: 2.842 ± 0.051	5.706ThrLeu: 5.706 ± 0.075	1.488ThrMet: 1.488 ± 0.04	2.186ThrAsn: 2.186 ± 0.044	2.387ThrPro: 2.387 ± 0.043	1.745ThrGln: 1.745 ± 0.034	2.051ThrArg: 2.051 ± 0.045	3.691ThrSer: 3.691 ± 0.045	3.1ThrThr: 3.1 ± 0.062	4.228ThrVal: 4.228 ± 0.054	0.612ThrTrp: 0.612 ± 0.017	1.932ThrTyr: 1.932 ± 0.039	0.0ThrXaa: 0.0 ± 0.0
Val	4.983ValAla: 4.983 ± 0.067	0.615ValCys: 0.615 ± 0.018	3.7ValAsp: 3.7 ± 0.057	4.706ValGlu: 4.706 ± 0.081	2.876ValPhe: 2.876 ± 0.046	4.675ValGly: 4.675 ± 0.065	1.478ValHis: 1.478 ± 0.03	5.542ValIle: 5.542 ± 0.07	4.151ValLys: 4.151 ± 0.06	7.081ValLeu: 7.081 ± 0.072	2.124ValMet: 2.124 ± 0.036	3.092ValAsn: 3.092 ± 0.057	2.694ValPro: 2.694 ± 0.047	2.634ValGln: 2.634 ± 0.051	2.943ValArg: 2.943 ± 0.046	5.039ValSer: 5.039 ± 0.075	4.332ValThr: 4.332 ± 0.061	5.131ValVal: 5.131 ± 0.077	0.756ValTrp: 0.756 ± 0.025	2.371ValTyr: 2.371 ± 0.039	0.0ValXaa: 0.0 ± 0.0
Trp	0.646TrpAla: 0.646 ± 0.02	0.102TrpCys: 0.102 ± 0.009	0.632TrpAsp: 0.632 ± 0.023	0.701TrpGlu: 0.701 ± 0.024	0.545TrpPhe: 0.545 ± 0.018	0.77TrpGly: 0.77 ± 0.024	0.236TrpHis: 0.236 ± 0.014	1.023TrpIle: 1.023 ± 0.03	0.68TrpLys: 0.68 ± 0.023	1.305TrpLeu: 1.305 ± 0.037	0.413TrpMet: 0.413 ± 0.018	0.667TrpAsn: 0.667 ± 0.024	0.268TrpPro: 0.268 ± 0.016	0.406TrpGln: 0.406 ± 0.017	0.519TrpArg: 0.519 ± 0.02	0.803TrpSer: 0.803 ± 0.023	0.599TrpThr: 0.599 ± 0.023	0.725TrpVal: 0.725 ± 0.025	0.171TrpTrp: 0.171 ± 0.012	0.355TrpTyr: 0.355 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.254TyrAla: 2.254 ± 0.044	0.33TyrCys: 0.33 ± 0.015	1.95TyrAsp: 1.95 ± 0.041	2.385TyrGlu: 2.385 ± 0.044	1.636TyrPhe: 1.636 ± 0.031	2.495TyrGly: 2.495 ± 0.043	0.817TyrHis: 0.817 ± 0.026	2.392TyrIle: 2.392 ± 0.042	1.904TyrLys: 1.904 ± 0.038	3.545TyrLeu: 3.545 ± 0.051	1.032TyrMet: 1.032 ± 0.031	1.53TyrAsn: 1.53 ± 0.043	1.501TyrPro: 1.501 ± 0.042	1.364TyrGln: 1.364 ± 0.033	1.922TyrArg: 1.922 ± 0.036	2.399TyrSer: 2.399 ± 0.046	1.925TyrThr: 1.925 ± 0.041	2.463TyrVal: 2.463 ± 0.048	0.396TyrTrp: 0.396 ± 0.019	1.464TyrTyr: 1.464 ± 0.036	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4588 proteins (1412272 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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