Amino acid dipepetide frequency for Nitrospira sp. SCGC AG-212-E16

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.95AlaAla: 9.95 ± 0.173
1.109AlaCys: 1.109 ± 0.048
4.628AlaAsp: 4.628 ± 0.095
5.799AlaGlu: 5.799 ± 0.115
3.211AlaPhe: 3.211 ± 0.075
7.712AlaGly: 7.712 ± 0.125
2.096AlaHis: 2.096 ± 0.059
5.481AlaIle: 5.481 ± 0.106
4.582AlaLys: 4.582 ± 0.115
10.444AlaLeu: 10.444 ± 0.173
2.697AlaMet: 2.697 ± 0.077
2.318AlaAsn: 2.318 ± 0.068
3.91AlaPro: 3.91 ± 0.122
3.879AlaGln: 3.879 ± 0.11
5.91AlaArg: 5.91 ± 0.108
5.441AlaSer: 5.441 ± 0.095
5.267AlaThr: 5.267 ± 0.096
7.436AlaVal: 7.436 ± 0.134
1.272AlaTrp: 1.272 ± 0.049
2.422AlaTyr: 2.422 ± 0.059
0.0AlaXaa: 0.0 ± 0.0
Cys
0.945CysAla: 0.945 ± 0.04
0.165CysCys: 0.165 ± 0.019
0.514CysAsp: 0.514 ± 0.032
0.563CysGlu: 0.563 ± 0.034
0.339CysPhe: 0.339 ± 0.024
0.918CysGly: 0.918 ± 0.038
0.386CysHis: 0.386 ± 0.054
0.388CysIle: 0.388 ± 0.029
0.349CysLys: 0.349 ± 0.026
1.086CysLeu: 1.086 ± 0.046
0.229CysMet: 0.229 ± 0.022
0.219CysAsn: 0.219 ± 0.021
0.601CysPro: 0.601 ± 0.033
0.398CysGln: 0.398 ± 0.025
0.791CysArg: 0.791 ± 0.039
0.634CysSer: 0.634 ± 0.036
0.49CysThr: 0.49 ± 0.033
0.749CysVal: 0.749 ± 0.043
0.113CysTrp: 0.113 ± 0.014
0.271CysTyr: 0.271 ± 0.025
0.0CysXaa: 0.0 ± 0.0
Asp
4.692AspAla: 4.692 ± 0.091
0.516AspCys: 0.516 ± 0.028
2.285AspAsp: 2.285 ± 0.077
3.316AspGlu: 3.316 ± 0.09
1.772AspPhe: 1.772 ± 0.051
3.851AspGly: 3.851 ± 0.086
1.371AspHis: 1.371 ± 0.049
2.596AspIle: 2.596 ± 0.079
1.925AspLys: 1.925 ± 0.061
5.757AspLeu: 5.757 ± 0.104
1.065AspMet: 1.065 ± 0.043
1.183AspAsn: 1.183 ± 0.049
3.071AspPro: 3.071 ± 0.086
2.256AspGln: 2.256 ± 0.062
4.184AspArg: 4.184 ± 0.102
2.537AspSer: 2.537 ± 0.058
2.511AspThr: 2.511 ± 0.071
3.84AspVal: 3.84 ± 0.079
0.749AspTrp: 0.749 ± 0.042
1.491AspTyr: 1.491 ± 0.057
0.0AspXaa: 0.0 ± 0.0
Glu
6.304GluAla: 6.304 ± 0.118
0.488GluCys: 0.488 ± 0.036
2.61GluAsp: 2.61 ± 0.07
3.879GluGlu: 3.879 ± 0.094
2.165GluPhe: 2.165 ± 0.056
4.438GluGly: 4.438 ± 0.086
1.437GluHis: 1.437 ± 0.05
3.331GluIle: 3.331 ± 0.095
2.914GluLys: 2.914 ± 0.076
6.092GluLeu: 6.092 ± 0.126
1.519GluMet: 1.519 ± 0.056
1.448GluAsn: 1.448 ± 0.055
2.466GluPro: 2.466 ± 0.079
3.133GluGln: 3.133 ± 0.078
4.749GluArg: 4.749 ± 0.11
3.373GluSer: 3.373 ± 0.079
3.298GluThr: 3.298 ± 0.075
4.37GluVal: 4.37 ± 0.103
0.935GluTrp: 0.935 ± 0.038
1.522GluTyr: 1.522 ± 0.05
0.0GluXaa: 0.0 ± 0.0
Phe
3.124PheAla: 3.124 ± 0.073
0.417PheCys: 0.417 ± 0.026
2.077PheAsp: 2.077 ± 0.064
2.063PheGlu: 2.063 ± 0.06
1.578PhePhe: 1.578 ± 0.053
3.06PheGly: 3.06 ± 0.082
0.945PheHis: 0.945 ± 0.042
1.738PheIle: 1.738 ± 0.06
1.397PheLys: 1.397 ± 0.052
3.863PheLeu: 3.863 ± 0.083
0.841PheMet: 0.841 ± 0.038
1.11PheAsn: 1.11 ± 0.047
1.769PhePro: 1.769 ± 0.066
1.204PheGln: 1.204 ± 0.044
2.356PheArg: 2.356 ± 0.061
2.452PheSer: 2.452 ± 0.064
2.325PheThr: 2.325 ± 0.073
2.704PheVal: 2.704 ± 0.072
0.546PheTrp: 0.546 ± 0.028
0.999PheTyr: 0.999 ± 0.042
0.0PheXaa: 0.0 ± 0.0
Gly
6.612GlyAla: 6.612 ± 0.133
0.954GlyCys: 0.954 ± 0.043
3.67GlyAsp: 3.67 ± 0.083
4.384GlyGlu: 4.384 ± 0.095
3.025GlyPhe: 3.025 ± 0.073
6.042GlyGly: 6.042 ± 0.142
1.957GlyHis: 1.957 ± 0.06
4.605GlyIle: 4.605 ± 0.09
3.875GlyLys: 3.875 ± 0.099
8.358GlyLeu: 8.358 ± 0.135
2.228GlyMet: 2.228 ± 0.062
2.188GlyAsn: 2.188 ± 0.074
2.919GlyPro: 2.919 ± 0.078
3.149GlyGln: 3.149 ± 0.082
4.985GlyArg: 4.985 ± 0.103
4.732GlySer: 4.732 ± 0.105
4.961GlyThr: 4.961 ± 0.092
5.849GlyVal: 5.849 ± 0.114
1.314GlyTrp: 1.314 ± 0.052
2.188GlyTyr: 2.188 ± 0.064
0.0GlyXaa: 0.0 ± 0.0
His
2.259HisAla: 2.259 ± 0.062
0.29HisCys: 0.29 ± 0.021
1.312HisAsp: 1.312 ± 0.049
1.418HisGlu: 1.418 ± 0.046
0.864HisPhe: 0.864 ± 0.041
1.944HisGly: 1.944 ± 0.065
0.784HisHis: 0.784 ± 0.043
1.204HisIle: 1.204 ± 0.05
0.709HisLys: 0.709 ± 0.042
2.48HisLeu: 2.48 ± 0.072
0.549HisMet: 0.549 ± 0.03
0.641HisAsn: 0.641 ± 0.035
1.625HisPro: 1.625 ± 0.06
1.025HisGln: 1.025 ± 0.044
1.689HisArg: 1.689 ± 0.059
1.209HisSer: 1.209 ± 0.047
1.194HisThr: 1.194 ± 0.051
1.826HisVal: 1.826 ± 0.049
0.318HisTrp: 0.318 ± 0.023
0.669HisTyr: 0.669 ± 0.036
0.0HisXaa: 0.0 ± 0.0
Ile
5.189IleAla: 5.189 ± 0.085
0.494IleCys: 0.494 ± 0.031
3.097IleAsp: 3.097 ± 0.09
3.806IleGlu: 3.806 ± 0.078
1.731IlePhe: 1.731 ± 0.055
4.8IleGly: 4.8 ± 0.089
1.31IleHis: 1.31 ± 0.054
2.502IleIle: 2.502 ± 0.071
2.176IleLys: 2.176 ± 0.077
5.173IleLeu: 5.173 ± 0.092
1.086IleMet: 1.086 ± 0.045
1.534IleAsn: 1.534 ± 0.056
2.904IlePro: 2.904 ± 0.077
2.037IleGln: 2.037 ± 0.067
3.555IleArg: 3.555 ± 0.081
2.963IleSer: 2.963 ± 0.072
3.142IleThr: 3.142 ± 0.081
4.414IleVal: 4.414 ± 0.092
0.558IleTrp: 0.558 ± 0.036
1.159IleTyr: 1.159 ± 0.043
0.0IleXaa: 0.0 ± 0.0
Lys
4.24LysAla: 4.24 ± 0.098
0.276LysCys: 0.276 ± 0.022
2.636LysAsp: 2.636 ± 0.085
3.069LysGlu: 3.069 ± 0.079
1.208LysPhe: 1.208 ± 0.047
3.453LysGly: 3.453 ± 0.094
0.872LysHis: 0.872 ± 0.045
2.454LysIle: 2.454 ± 0.076
2.508LysLys: 2.508 ± 0.081
3.835LysLeu: 3.835 ± 0.089
1.145LysMet: 1.145 ± 0.048
1.295LysAsn: 1.295 ± 0.052
2.252LysPro: 2.252 ± 0.077
1.95LysGln: 1.95 ± 0.054
2.798LysArg: 2.798 ± 0.07
2.407LysSer: 2.407 ± 0.072
2.652LysThr: 2.652 ± 0.064
3.098LysVal: 3.098 ± 0.078
0.457LysTrp: 0.457 ± 0.031
0.98LysTyr: 0.98 ± 0.043
0.0LysXaa: 0.0 ± 0.0
Leu
11.012LeuAla: 11.012 ± 0.146
1.182LeuCys: 1.182 ± 0.046
5.554LeuAsp: 5.554 ± 0.109
5.967LeuGlu: 5.967 ± 0.111
3.934LeuPhe: 3.934 ± 0.1
7.604LeuGly: 7.604 ± 0.142
2.37LeuHis: 2.37 ± 0.062
5.232LeuIle: 5.232 ± 0.109
4.671LeuLys: 4.671 ± 0.097
10.849LeuLeu: 10.849 ± 0.21
2.311LeuMet: 2.311 ± 0.072
2.786LeuAsn: 2.786 ± 0.068
5.272LeuPro: 5.272 ± 0.109
3.674LeuGln: 3.674 ± 0.087
7.099LeuArg: 7.099 ± 0.132
6.843LeuSer: 6.843 ± 0.102
6.569LeuThr: 6.569 ± 0.106
7.98LeuVal: 7.98 ± 0.132
1.267LeuTrp: 1.267 ± 0.051
2.568LeuTyr: 2.568 ± 0.082
0.0LeuXaa: 0.0 ± 0.0
Met
2.746MetAla: 2.746 ± 0.076
0.158MetCys: 0.158 ± 0.016
1.183MetAsp: 1.183 ± 0.043
1.338MetGlu: 1.338 ± 0.046
0.697MetPhe: 0.697 ± 0.033
1.802MetGly: 1.802 ± 0.059
0.421MetHis: 0.421 ± 0.028
1.312MetIle: 1.312 ± 0.049
1.493MetLys: 1.493 ± 0.063
2.356MetLeu: 2.356 ± 0.074
0.664MetMet: 0.664 ± 0.035
0.813MetAsn: 0.813 ± 0.036
1.409MetPro: 1.409 ± 0.053
0.931MetGln: 0.931 ± 0.036
1.573MetArg: 1.573 ± 0.048
1.602MetSer: 1.602 ± 0.054
1.774MetThr: 1.774 ± 0.056
1.805MetVal: 1.805 ± 0.059
0.254MetTrp: 0.254 ± 0.018
0.443MetTyr: 0.443 ± 0.024
0.0MetXaa: 0.0 ± 0.0
Asn
2.292AsnAla: 2.292 ± 0.068
0.297AsnCys: 0.297 ± 0.021
1.277AsnAsp: 1.277 ± 0.048
1.397AsnGlu: 1.397 ± 0.046
1.017AsnPhe: 1.017 ± 0.043
2.233AsnGly: 2.233 ± 0.075
0.648AsnHis: 0.648 ± 0.038
1.451AsnIle: 1.451 ± 0.061
0.954AsnLys: 0.954 ± 0.048
2.855AsnLeu: 2.855 ± 0.075
0.655AsnMet: 0.655 ± 0.034
0.836AsnAsn: 0.836 ± 0.051
2.017AsnPro: 2.017 ± 0.068
1.182AsnGln: 1.182 ± 0.056
2.011AsnArg: 2.011 ± 0.056
1.475AsnSer: 1.475 ± 0.058
1.378AsnThr: 1.378 ± 0.049
2.155AsnVal: 2.155 ± 0.069
0.372AsnTrp: 0.372 ± 0.027
0.735AsnTyr: 0.735 ± 0.039
0.0AsnXaa: 0.0 ± 0.0
Pro
4.574ProAla: 4.574 ± 0.107
0.421ProCys: 0.421 ± 0.023
3.039ProAsp: 3.039 ± 0.07
3.196ProGlu: 3.196 ± 0.081
1.993ProPhe: 1.993 ± 0.053
3.811ProGly: 3.811 ± 0.093
1.225ProHis: 1.225 ± 0.049
2.784ProIle: 2.784 ± 0.074
2.033ProLys: 2.033 ± 0.062
4.972ProLeu: 4.972 ± 0.116
1.315ProMet: 1.315 ± 0.052
1.522ProAsn: 1.522 ± 0.048
2.603ProPro: 2.603 ± 0.094
1.8ProGln: 1.8 ± 0.054
2.495ProArg: 2.495 ± 0.064
3.489ProSer: 3.489 ± 0.079
2.954ProThr: 2.954 ± 0.087
3.87ProVal: 3.87 ± 0.085
0.699ProTrp: 0.699 ± 0.036
1.298ProTyr: 1.298 ± 0.052
0.0ProXaa: 0.0 ± 0.0
Gln
4.466GlnAla: 4.466 ± 0.102
0.349GlnCys: 0.349 ± 0.026
2.016GlnAsp: 2.016 ± 0.068
2.535GlnGlu: 2.535 ± 0.071
1.52GlnPhe: 1.52 ± 0.051
3.003GlnGly: 3.003 ± 0.076
0.989GlnHis: 0.989 ± 0.045
2.03GlnIle: 2.03 ± 0.058
1.484GlnLys: 1.484 ± 0.051
4.054GlnLeu: 4.054 ± 0.091
0.937GlnMet: 0.937 ± 0.035
0.931GlnAsn: 0.931 ± 0.043
1.972GlnPro: 1.972 ± 0.058
2.031GlnGln: 2.031 ± 0.067
2.789GlnArg: 2.789 ± 0.065
2.262GlnSer: 2.262 ± 0.061
2.144GlnThr: 2.144 ± 0.066
2.991GlnVal: 2.991 ± 0.073
0.633GlnTrp: 0.633 ± 0.036
1.013GlnTyr: 1.013 ± 0.048
0.0GlnXaa: 0.0 ± 0.0
Arg
5.886ArgAla: 5.886 ± 0.118
0.636ArgCys: 0.636 ± 0.036
3.387ArgAsp: 3.387 ± 0.079
4.403ArgGlu: 4.403 ± 0.089
2.713ArgPhe: 2.713 ± 0.068
4.249ArgGly: 4.249 ± 0.091
1.642ArgHis: 1.642 ± 0.059
3.965ArgIle: 3.965 ± 0.075
2.798ArgLys: 2.798 ± 0.074
7.587ArgLeu: 7.587 ± 0.146
1.767ArgMet: 1.767 ± 0.062
1.727ArgAsn: 1.727 ± 0.054
2.907ArgPro: 2.907 ± 0.071
2.824ArgGln: 2.824 ± 0.071
4.664ArgArg: 4.664 ± 0.117
3.621ArgSer: 3.621 ± 0.08
3.649ArgThr: 3.649 ± 0.079
4.956ArgVal: 4.956 ± 0.088
1.109ArgTrp: 1.109 ± 0.04
2.042ArgTyr: 2.042 ± 0.058
0.0ArgXaa: 0.0 ± 0.0
Ser
5.194SerAla: 5.194 ± 0.096
0.619SerCys: 0.619 ± 0.038
2.942SerAsp: 2.942 ± 0.076
3.269SerGlu: 3.269 ± 0.078
2.325SerPhe: 2.325 ± 0.07
5.083SerGly: 5.083 ± 0.119
1.505SerHis: 1.505 ± 0.05
3.079SerIle: 3.079 ± 0.082
2.292SerLys: 2.292 ± 0.071
6.478SerLeu: 6.478 ± 0.116
1.595SerMet: 1.595 ± 0.049
1.628SerAsn: 1.628 ± 0.061
3.333SerPro: 3.333 ± 0.08
2.198SerGln: 2.198 ± 0.06
3.736SerArg: 3.736 ± 0.09
3.7SerSer: 3.7 ± 0.091
3.286SerThr: 3.286 ± 0.085
4.442SerVal: 4.442 ± 0.097
0.782SerTrp: 0.782 ± 0.042
1.484SerTyr: 1.484 ± 0.054
0.0SerXaa: 0.0 ± 0.0
Thr
5.396ThrAla: 5.396 ± 0.107
0.504ThrCys: 0.504 ± 0.033
2.923ThrAsp: 2.923 ± 0.067
2.991ThrGlu: 2.991 ± 0.08
2.115ThrPhe: 2.115 ± 0.065
4.951ThrGly: 4.951 ± 0.109
1.408ThrHis: 1.408 ± 0.054
3.687ThrIle: 3.687 ± 0.077
2.384ThrLys: 2.384 ± 0.074
6.052ThrLeu: 6.052 ± 0.118
1.428ThrMet: 1.428 ± 0.05
1.618ThrAsn: 1.618 ± 0.055
3.375ThrPro: 3.375 ± 0.089
2.061ThrGln: 2.061 ± 0.069
3.133ThrArg: 3.133 ± 0.072
3.246ThrSer: 3.246 ± 0.084
3.557ThrThr: 3.557 ± 0.097
5.158ThrVal: 5.158 ± 0.098
0.707ThrTrp: 0.707 ± 0.035
1.493ThrTyr: 1.493 ± 0.048
0.0ThrXaa: 0.0 ± 0.0
Val
7.234ValAla: 7.234 ± 0.122
0.798ValCys: 0.798 ± 0.04
3.667ValAsp: 3.667 ± 0.07
4.709ValGlu: 4.709 ± 0.109
2.702ValPhe: 2.702 ± 0.069
5.893ValGly: 5.893 ± 0.101
1.62ValHis: 1.62 ± 0.062
4.012ValIle: 4.012 ± 0.088
3.395ValLys: 3.395 ± 0.083
8.178ValLeu: 8.178 ± 0.122
1.892ValMet: 1.892 ± 0.05
2.262ValAsn: 2.262 ± 0.07
3.839ValPro: 3.839 ± 0.09
2.766ValGln: 2.766 ± 0.072
5.046ValArg: 5.046 ± 0.111
4.728ValSer: 4.728 ± 0.088
4.975ValThr: 4.975 ± 0.11
6.473ValVal: 6.473 ± 0.124
0.902ValTrp: 0.902 ± 0.043
1.79ValTyr: 1.79 ± 0.06
0.0ValXaa: 0.0 ± 0.0
Trp
1.123TrpAla: 1.123 ± 0.048
0.191TrpCys: 0.191 ± 0.018
0.608TrpAsp: 0.608 ± 0.036
0.839TrpGlu: 0.839 ± 0.04
0.565TrpPhe: 0.565 ± 0.032
0.989TrpGly: 0.989 ± 0.042
0.325TrpHis: 0.325 ± 0.026
0.704TrpIle: 0.704 ± 0.038
0.686TrpLys: 0.686 ± 0.034
1.536TrpLeu: 1.536 ± 0.058
0.379TrpMet: 0.379 ± 0.029
0.494TrpAsn: 0.494 ± 0.03
0.549TrpPro: 0.549 ± 0.031
0.56TrpGln: 0.56 ± 0.033
0.785TrpArg: 0.785 ± 0.037
0.867TrpSer: 0.867 ± 0.042
0.772TrpThr: 0.772 ± 0.049
1.013TrpVal: 1.013 ± 0.044
0.278TrpTrp: 0.278 ± 0.022
0.41TrpTyr: 0.41 ± 0.036
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.325TyrAla: 2.325 ± 0.07
0.285TyrCys: 0.285 ± 0.028
1.545TyrAsp: 1.545 ± 0.051
1.533TyrGlu: 1.533 ± 0.059
1.095TyrPhe: 1.095 ± 0.049
2.162TyrGly: 2.162 ± 0.067
0.7TyrHis: 0.7 ± 0.034
1.072TyrIle: 1.072 ± 0.045
0.957TyrLys: 0.957 ± 0.042
2.737TyrLeu: 2.737 ± 0.073
0.469TyrMet: 0.469 ± 0.03
0.683TyrAsn: 0.683 ± 0.036
1.3TyrPro: 1.3 ± 0.048
1.093TyrGln: 1.093 ± 0.041
2.183TyrArg: 2.183 ± 0.061
1.387TyrSer: 1.387 ± 0.054
1.288TyrThr: 1.288 ± 0.048
1.753TyrVal: 1.753 ± 0.06
0.41TyrTrp: 0.41 ± 0.029
0.851TyrTyr: 0.851 ± 0.048
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2093 proteins (575471 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski