Changjiang tombus-like virus 17
Average proteome isoelectric point is 8.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KFU6|A0A1L3KFU6_9VIRU RNA-directed RNA polymerase OS=Changjiang tombus-like virus 17 OX=1922810 PE=4 SV=1
MM1 pKa = 6.95 YY2 pKa = 10.19 RR3 pKa = 11.84 GFRR6 pKa = 11.84 RR7 pKa = 11.84 RR8 pKa = 11.84 RR9 pKa = 11.84 YY10 pKa = 8.82 EE11 pKa = 3.52 QAKK14 pKa = 9.94 QNLSISGPLIDD25 pKa = 4.99 SRR27 pKa = 11.84 VKK29 pKa = 10.34 MFVKK33 pKa = 10.04 MEE35 pKa = 4.56 GIKK38 pKa = 10.61 FSSEE42 pKa = 4.17 KK43 pKa = 10.46 INPSCRR49 pKa = 11.84 AIQFRR54 pKa = 11.84 SPEE57 pKa = 3.88 YY58 pKa = 9.87 QLQVACFIKK67 pKa = 10.49 PIEE70 pKa = 4.26 HH71 pKa = 7.32 LLYY74 pKa = 10.4 CSSGPYY80 pKa = 9.55 PYY82 pKa = 9.8 PASQFIAKK90 pKa = 9.36 NLNGVEE96 pKa = 4.02 RR97 pKa = 11.84 AQLLWEE103 pKa = 4.96 KK104 pKa = 10.4 YY105 pKa = 10.48 ANLPGCEE112 pKa = 3.91 ILMLDD117 pKa = 3.9 ASRR120 pKa = 11.84 FDD122 pKa = 3.38 GHH124 pKa = 5.07 VTPRR128 pKa = 11.84 LNSIEE133 pKa = 3.97 EE134 pKa = 4.04 HH135 pKa = 7.04 FYY137 pKa = 8.6 TTICPDD143 pKa = 3.29 PFFRR147 pKa = 11.84 SLLKK151 pKa = 10.36 ARR153 pKa = 11.84 KK154 pKa = 8.72 VNKK157 pKa = 10.04 GSAKK161 pKa = 10.3 GADD164 pKa = 3.66 FSVSYY169 pKa = 10.01 SLKK172 pKa = 8.99 GGRR175 pKa = 11.84 MSGDD179 pKa = 3.2 MDD181 pKa = 4.05 TASSNCLLMSSMLALLGSTSCLSYY205 pKa = 11.42 DD206 pKa = 3.84 FLVDD210 pKa = 3.33 GDD212 pKa = 4.49 DD213 pKa = 4.08 SVFFYY218 pKa = 11.03 LGDD221 pKa = 3.78 TLTDD225 pKa = 3.34 DD226 pKa = 4.43 HH227 pKa = 8.01 IKK229 pKa = 10.8 EE230 pKa = 4.24 FFLEE234 pKa = 4.69 CGMEE238 pKa = 3.89 MKK240 pKa = 9.81 IDD242 pKa = 3.25 KK243 pKa = 8.43 RR244 pKa = 11.84 TRR246 pKa = 11.84 DD247 pKa = 3.33 FWSLDD252 pKa = 3.34 FCQGKK257 pKa = 8.47 PVSLPGGISLVRR269 pKa = 11.84 DD270 pKa = 4.18 PIKK273 pKa = 11.08 VMSKK277 pKa = 10.76 AGINDD282 pKa = 3.63 KK283 pKa = 10.9 FSDD286 pKa = 3.56 PLLRR290 pKa = 11.84 CRR292 pKa = 11.84 ILEE295 pKa = 4.33 VICLGEE301 pKa = 3.87 LSLVKK306 pKa = 10.42 GCPILQHH313 pKa = 6.3 FFDD316 pKa = 4.49 RR317 pKa = 11.84 LLYY320 pKa = 9.35 IARR323 pKa = 11.84 RR324 pKa = 11.84 SLGKK328 pKa = 10.23 KK329 pKa = 8.97 KK330 pKa = 10.09 RR331 pKa = 11.84 KK332 pKa = 7.83 KK333 pKa = 10.72 LKK335 pKa = 9.44 MEE337 pKa = 3.69 WMSYY341 pKa = 6.9 RR342 pKa = 11.84 QRR344 pKa = 11.84 NEE346 pKa = 3.93 FRR348 pKa = 11.84 GEE350 pKa = 3.08 WWKK353 pKa = 11.3 RR354 pKa = 11.84 EE355 pKa = 3.91 VLPITPEE362 pKa = 3.59 ARR364 pKa = 11.84 EE365 pKa = 4.04 TLSRR369 pKa = 11.84 AWGISIEE376 pKa = 4.22 EE377 pKa = 3.93 QLSLEE382 pKa = 4.08 KK383 pKa = 10.87 QIDD386 pKa = 3.96 GFDD389 pKa = 4.45 FDD391 pKa = 5.18 PMSTPIRR398 pKa = 11.84 GEE400 pKa = 4.0 GVDD403 pKa = 3.56 VSNWLFDD410 pKa = 3.33 AFTRR414 pKa = 11.84 EE415 pKa = 4.26 SFF417 pKa = 3.4
Molecular weight: 47.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.514
IPC2_protein 7.863
IPC_protein 7.673
Toseland 7.249
ProMoST 8.273
Dawson 8.434
Bjellqvist 8.785
Wikipedia 8.346
Rodwell 8.463
Grimsley 7.22
Solomon 8.492
Lehninger 8.507
Nozaki 9.033
DTASelect 8.507
Thurlkill 8.565
EMBOSS 8.624
Sillero 8.902
Patrickios 4.52
IPC_peptide 8.492
IPC2_peptide 7.688
IPC2.peptide.svr19 7.927
Protein with the highest isoelectric point:
>tr|A0A1L3KFU6|A0A1L3KFU6_9VIRU RNA-directed RNA polymerase OS=Changjiang tombus-like virus 17 OX=1922810 PE=4 SV=1
MM1 pKa = 7.88 RR2 pKa = 11.84 FLTNVPSVYY11 pKa = 10.31 EE12 pKa = 4.19 GFSGRR17 pKa = 11.84 LRR19 pKa = 11.84 SWITEE24 pKa = 3.45 NRR26 pKa = 11.84 RR27 pKa = 11.84 NIILGSFCLIASGLTFRR44 pKa = 11.84 KK45 pKa = 8.39 TSQLTLLFRR54 pKa = 11.84 SLLGLLGAGLSFTLGNNLTNLVSLQLEE81 pKa = 4.34 NKK83 pKa = 10.09 AKK85 pKa = 9.57 IARR88 pKa = 11.84 RR89 pKa = 11.84 GTRR92 pKa = 11.84 GKK94 pKa = 9.31 MRR96 pKa = 11.84 AIRR99 pKa = 11.84 HH100 pKa = 5.27 RR101 pKa = 11.84 RR102 pKa = 11.84 VPLTNLHH109 pKa = 6.51 FEE111 pKa = 4.28 VEE113 pKa = 4.53 SLCFDD118 pKa = 4.14 GLRR121 pKa = 11.84 NSSDD125 pKa = 3.15 VTDD128 pKa = 3.65 IARR131 pKa = 11.84 RR132 pKa = 11.84 LKK134 pKa = 10.23 QIAMSSGLEE143 pKa = 4.16 VYY145 pKa = 10.94 SDD147 pKa = 3.98 DD148 pKa = 3.53 GTVNKK153 pKa = 9.95 VPVKK157 pKa = 9.78 DD158 pKa = 4.12 LNMALVDD165 pKa = 4.1 SVVRR169 pKa = 11.84 DD170 pKa = 4.02 CVLHH174 pKa = 7.75 ISDD177 pKa = 5.08 PDD179 pKa = 3.51 VLRR182 pKa = 11.84 DD183 pKa = 3.57 IEE185 pKa = 4.18 VDD187 pKa = 3.16 AEE189 pKa = 4.05 NRR191 pKa = 11.84 DD192 pKa = 3.32 MAMRR196 pKa = 11.84 RR197 pKa = 11.84 LLSLGNAGQILSGWEE212 pKa = 3.76 HH213 pKa = 6.44 LKK215 pKa = 10.57 ISALKK220 pKa = 10.34 CGAAILGRR228 pKa = 11.84 SYY230 pKa = 11.41 LVGRR234 pKa = 11.84 NN235 pKa = 3.28
Molecular weight: 26.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.241
IPC2_protein 9.18
IPC_protein 9.399
Toseland 10.204
ProMoST 9.823
Dawson 10.335
Bjellqvist 10.014
Wikipedia 10.482
Rodwell 10.599
Grimsley 10.379
Solomon 10.394
Lehninger 10.379
Nozaki 10.248
DTASelect 9.984
Thurlkill 10.218
EMBOSS 10.599
Sillero 10.262
Patrickios 10.321
IPC_peptide 10.409
IPC2_peptide 8.975
IPC2.peptide.svr19 8.168
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
994
235
417
331.3
36.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.841 ± 1.425
1.911 ± 0.456
5.332 ± 0.485
4.628 ± 0.74
5.131 ± 0.747
6.64 ± 0.58
1.107 ± 0.254
5.634 ± 0.169
5.332 ± 0.797
11.066 ± 1.581
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.918 ± 0.211
4.225 ± 0.821
4.93 ± 1.288
2.213 ± 0.315
6.942 ± 1.39
9.557 ± 0.237
6.237 ± 1.986
5.231 ± 0.774
1.207 ± 0.223
2.918 ± 0.61
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here