Kluyvera phage Kvp1
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B6Z9G6|B6Z9G6_9CAUD Exonuclease OS=Kluyvera phage Kvp1 OX=47049 GN=6 PE=4 SV=1
MM1 pKa = 7.48 SLHH4 pKa = 6.41 TDD6 pKa = 2.91 NVTVTRR12 pKa = 11.84 EE13 pKa = 3.88 AWNDD17 pKa = 3.27 MQSYY21 pKa = 9.23 IQSLEE26 pKa = 3.7 KK27 pKa = 10.83 DD28 pKa = 3.57 QEE30 pKa = 4.11 FLNALRR36 pKa = 11.84 ACGVDD41 pKa = 2.95 NWDD44 pKa = 4.2 GYY46 pKa = 11.38 SDD48 pKa = 4.9 AIEE51 pKa = 4.12 MVYY54 pKa = 11.02 GEE56 pKa = 5.02 DD57 pKa = 4.95 DD58 pKa = 3.52 EE59 pKa = 5.21
Molecular weight: 6.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.761
IPC2_protein 3.77
IPC_protein 3.694
Toseland 3.49
ProMoST 3.834
Dawson 3.694
Bjellqvist 3.935
Wikipedia 3.643
Rodwell 3.528
Grimsley 3.414
Solomon 3.656
Lehninger 3.617
Nozaki 3.834
DTASelect 4.012
Thurlkill 3.567
EMBOSS 3.656
Sillero 3.821
Patrickios 1.863
IPC_peptide 3.656
IPC2_peptide 3.783
IPC2.peptide.svr19 3.774
Protein with the highest isoelectric point:
>tr|B6Z9E5|B6Z9E5_9CAUD Protein kinase OS=Kluyvera phage Kvp1 OX=47049 GN=0.7 PE=4 SV=1
MM1 pKa = 7.49 LQRR4 pKa = 11.84 TDD6 pKa = 4.06 DD7 pKa = 3.78 LKK9 pKa = 11.05 KK10 pKa = 10.93 GYY12 pKa = 7.77 MQNGTLYY19 pKa = 10.13 AANRR23 pKa = 11.84 RR24 pKa = 11.84 IVRR27 pKa = 11.84 TWRR30 pKa = 11.84 EE31 pKa = 3.6 NNLEE35 pKa = 3.83 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.01 EE39 pKa = 3.86 QEE41 pKa = 3.11 RR42 pKa = 11.84 AAWHH46 pKa = 5.56 RR47 pKa = 11.84 RR48 pKa = 11.84 KK49 pKa = 10.41 EE50 pKa = 3.98 KK51 pKa = 11.01 VKK53 pKa = 10.13 AQKK56 pKa = 10.24 LAALEE61 pKa = 3.87 QALNNTLNNLSS72 pKa = 3.61
Molecular weight: 8.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.308
IPC2_protein 9.765
IPC_protein 10.584
Toseland 10.935
ProMoST 10.643
Dawson 10.994
Bjellqvist 10.701
Wikipedia 11.213
Rodwell 11.257
Grimsley 11.038
Solomon 11.155
Lehninger 11.125
Nozaki 10.906
DTASelect 10.701
Thurlkill 10.921
EMBOSS 11.345
Sillero 10.935
Patrickios 11.008
IPC_peptide 11.169
IPC2_peptide 9.428
IPC2.peptide.svr19 8.684
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
47
0
47
12360
26
1315
263.0
29.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.167 ± 0.421
0.995 ± 0.181
6.367 ± 0.227
7.29 ± 0.337
3.544 ± 0.184
8.058 ± 0.312
1.917 ± 0.162
4.765 ± 0.159
7.079 ± 0.346
7.929 ± 0.261
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.977 ± 0.194
4.644 ± 0.266
3.471 ± 0.206
3.819 ± 0.256
5.591 ± 0.228
5.542 ± 0.296
5.307 ± 0.197
6.642 ± 0.287
1.521 ± 0.211
3.374 ± 0.163
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here