Gigaspora margarita mitovirus 1
Average proteome isoelectric point is 9.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2L0VZH3|A0A2L0VZH3_9VIRU RNA-dependent RNA polymerase OS=Gigaspora margarita mitovirus 1 OX=2082665 PE=4 SV=1
MM1 pKa = 7.56 KK2 pKa = 10.45 ALLLSLIRR10 pKa = 11.84 VAKK13 pKa = 9.99 EE14 pKa = 3.48 RR15 pKa = 11.84 VTVRR19 pKa = 11.84 PVPLRR24 pKa = 11.84 RR25 pKa = 11.84 LNVKK29 pKa = 8.82 VWHH32 pKa = 6.68 HH33 pKa = 6.7 LFPALVSVGRR43 pKa = 11.84 LVIGRR48 pKa = 11.84 ISRR51 pKa = 11.84 DD52 pKa = 3.46 RR53 pKa = 11.84 IKK55 pKa = 11.15 VLAFWAKK62 pKa = 9.74 QCVAMIRR69 pKa = 11.84 KK70 pKa = 8.98 QGVKK74 pKa = 8.97 GTCLWLKK81 pKa = 8.85 TINVLIMASLPGSQLKK97 pKa = 9.72 SQSRR101 pKa = 11.84 AISKK105 pKa = 9.47 VAVSVSRR112 pKa = 11.84 DD113 pKa = 3.22 GLPRR117 pKa = 11.84 VIPKK121 pKa = 9.37 QDD123 pKa = 2.75 RR124 pKa = 11.84 RR125 pKa = 11.84 AIRR128 pKa = 11.84 AGDD131 pKa = 3.64 TNTIRR136 pKa = 11.84 LWLSLSGMYY145 pKa = 9.51 RR146 pKa = 11.84 VLEE149 pKa = 4.27 FPGKK153 pKa = 10.48 AKK155 pKa = 10.36 ISTITDD161 pKa = 4.1 PGPRR165 pKa = 11.84 ISPPLLSFFEE175 pKa = 4.47 TFLKK179 pKa = 10.8 RR180 pKa = 11.84 EE181 pKa = 4.24 FFRR184 pKa = 11.84 NLGDD188 pKa = 3.61 LTGLKK193 pKa = 8.83 VTGIDD198 pKa = 3.63 PAGLRR203 pKa = 11.84 PTPLPLVTSAAGSWVVPSGKK223 pKa = 9.94 YY224 pKa = 9.84 DD225 pKa = 3.58 LRR227 pKa = 11.84 VSSLGTAAPAAWNWVNGHH245 pKa = 5.95 WGEE248 pKa = 3.87 ALLRR252 pKa = 11.84 YY253 pKa = 9.3 SQAIGSWDD261 pKa = 3.69 TTNSWYY267 pKa = 10.74 RR268 pKa = 11.84 LVEE271 pKa = 3.72 EE272 pKa = 4.36 SARR275 pKa = 11.84 YY276 pKa = 9.42 SHH278 pKa = 6.99 FCSRR282 pKa = 11.84 MSRR285 pKa = 11.84 GRR287 pKa = 11.84 ISFKK291 pKa = 10.71 DD292 pKa = 3.76 EE293 pKa = 3.63 PAGKK297 pKa = 9.74 VRR299 pKa = 11.84 AFAMVDD305 pKa = 3.06 YY306 pKa = 7.77 WTQCSLKK313 pKa = 9.72 PLHH316 pKa = 7.13 DD317 pKa = 4.42 LLFSILKK324 pKa = 8.75 EE325 pKa = 3.96 IPQDD329 pKa = 3.53 GTFDD333 pKa = 3.29 QMAPAKK339 pKa = 9.66 EE340 pKa = 3.93 LLSVRR345 pKa = 11.84 RR346 pKa = 11.84 LATEE350 pKa = 4.13 TWWSLDD356 pKa = 3.19 LSAATDD362 pKa = 4.08 RR363 pKa = 11.84 FPLALQKK370 pKa = 11.0 LAMKK374 pKa = 10.04 YY375 pKa = 10.03 LVSEE379 pKa = 4.94 EE380 pKa = 4.36 YY381 pKa = 10.42 AAAWATLMVDD391 pKa = 3.68 RR392 pKa = 11.84 PFRR395 pKa = 11.84 LPVGYY400 pKa = 10.1 KK401 pKa = 10.25 PRR403 pKa = 11.84 DD404 pKa = 3.3 CRR406 pKa = 11.84 YY407 pKa = 10.46 AVGQPMGAYY416 pKa = 10.02 SSWAAFALTHH426 pKa = 6.24 HH427 pKa = 6.52 ATVQFAARR435 pKa = 11.84 LSGLSGWFRR444 pKa = 11.84 DD445 pKa = 3.91 YY446 pKa = 11.88 ALLGDD451 pKa = 5.49 DD452 pKa = 5.37 IIIANDD458 pKa = 3.46 RR459 pKa = 11.84 VAHH462 pKa = 6.7 KK463 pKa = 10.36 YY464 pKa = 9.75 RR465 pKa = 11.84 WLLSQLGVEE474 pKa = 4.48 CSLAKK479 pKa = 10.68 SMASNQRR486 pKa = 11.84 TFEE489 pKa = 3.79 FAKK492 pKa = 9.91 RR493 pKa = 11.84 VLFRR497 pKa = 11.84 GVDD500 pKa = 3.4 VSGFPWKK507 pKa = 10.3 LWRR510 pKa = 11.84 IGQRR514 pKa = 11.84 SLAAMISLAQRR525 pKa = 11.84 VSFGTAPLNLASLVKK540 pKa = 10.69 ALGAGMKK547 pKa = 8.64 ATSRR551 pKa = 11.84 AYY553 pKa = 8.52 TAWRR557 pKa = 11.84 HH558 pKa = 4.56 MPRR561 pKa = 11.84 GLKK564 pKa = 10.22 ALLIILTHH572 pKa = 5.99 PQANTFLSRR581 pKa = 11.84 QTWLDD586 pKa = 3.1 WLTAPGPVLPSGYY599 pKa = 10.4 KK600 pKa = 9.94 VDD602 pKa = 3.42 TSTWLTPWLSGFRR615 pKa = 11.84 EE616 pKa = 4.54 EE617 pKa = 4.64 YY618 pKa = 10.82 LNPALEE624 pKa = 4.1 FLEE627 pKa = 4.63 EE628 pKa = 4.31 EE629 pKa = 4.26 STLDD633 pKa = 4.6 LFEE636 pKa = 5.98 PDD638 pKa = 2.61 IDD640 pKa = 4.62 RR641 pKa = 11.84 RR642 pKa = 11.84 DD643 pKa = 3.56 AQGNLTRR650 pKa = 11.84 DD651 pKa = 3.32 SHH653 pKa = 7.64 YY654 pKa = 8.47 MAEE657 pKa = 4.97 FIRR660 pKa = 11.84 FRR662 pKa = 11.84 RR663 pKa = 11.84 AYY665 pKa = 10.04 LFSKK669 pKa = 10.74 LEE671 pKa = 3.99 SLVSVEE677 pKa = 3.7 RR678 pKa = 11.84 SKK680 pKa = 11.03 KK681 pKa = 9.26 IRR683 pKa = 11.84 QLEE686 pKa = 3.81 EE687 pKa = 3.26 RR688 pKa = 11.84 AAKK691 pKa = 10.15 IEE693 pKa = 4.08 ADD695 pKa = 3.55 LAHH698 pKa = 6.3 LQKK701 pKa = 11.08 LSISLQARR709 pKa = 11.84 NVSGMFNSMMKK720 pKa = 10.41 RR721 pKa = 11.84 LEE723 pKa = 3.92 EE724 pKa = 4.4 SIAAVPLPLPWLVWARR740 pKa = 11.84 EE741 pKa = 4.09 EE742 pKa = 4.4 NEE744 pKa = 3.65 NRR746 pKa = 11.84 RR747 pKa = 11.84 PATVLVSLWEE757 pKa = 3.88 RR758 pKa = 11.84 WRR760 pKa = 11.84 LRR762 pKa = 11.84 IHH764 pKa = 7.77 RR765 pKa = 11.84 SRR767 pKa = 11.84 KK768 pKa = 9.8 LPTHH772 pKa = 7.07 ANPQGEE778 pKa = 4.22 AVKK781 pKa = 10.79 ARR783 pKa = 11.84 FEE785 pKa = 4.65 KK786 pKa = 10.71 PFSTDD791 pKa = 3.09 FDD793 pKa = 4.06 EE794 pKa = 5.12
Molecular weight: 89.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.263
IPC2_protein 9.458
IPC_protein 9.765
Toseland 10.379
ProMoST 9.999
Dawson 10.511
Bjellqvist 10.175
Wikipedia 10.672
Rodwell 10.847
Grimsley 10.57
Solomon 10.555
Lehninger 10.54
Nozaki 10.365
DTASelect 10.16
Thurlkill 10.394
EMBOSS 10.774
Sillero 10.423
Patrickios 10.496
IPC_peptide 10.57
IPC2_peptide 8.887
IPC2.peptide.svr19 8.62
Protein with the highest isoelectric point:
>tr|A0A2L0VZH3|A0A2L0VZH3_9VIRU RNA-dependent RNA polymerase OS=Gigaspora margarita mitovirus 1 OX=2082665 PE=4 SV=1
MM1 pKa = 7.56 KK2 pKa = 10.45 ALLLSLIRR10 pKa = 11.84 VAKK13 pKa = 9.99 EE14 pKa = 3.48 RR15 pKa = 11.84 VTVRR19 pKa = 11.84 PVPLRR24 pKa = 11.84 RR25 pKa = 11.84 LNVKK29 pKa = 8.82 VWHH32 pKa = 6.68 HH33 pKa = 6.7 LFPALVSVGRR43 pKa = 11.84 LVIGRR48 pKa = 11.84 ISRR51 pKa = 11.84 DD52 pKa = 3.46 RR53 pKa = 11.84 IKK55 pKa = 11.15 VLAFWAKK62 pKa = 9.74 QCVAMIRR69 pKa = 11.84 KK70 pKa = 8.98 QGVKK74 pKa = 8.97 GTCLWLKK81 pKa = 8.85 TINVLIMASLPGSQLKK97 pKa = 9.72 SQSRR101 pKa = 11.84 AISKK105 pKa = 9.47 VAVSVSRR112 pKa = 11.84 DD113 pKa = 3.22 GLPRR117 pKa = 11.84 VIPKK121 pKa = 9.37 QDD123 pKa = 2.75 RR124 pKa = 11.84 RR125 pKa = 11.84 AIRR128 pKa = 11.84 AGDD131 pKa = 3.64 TNTIRR136 pKa = 11.84 LWLSLSGMYY145 pKa = 9.51 RR146 pKa = 11.84 VLEE149 pKa = 4.27 FPGKK153 pKa = 10.48 AKK155 pKa = 10.36 ISTITDD161 pKa = 4.1 PGPRR165 pKa = 11.84 ISPPLLSFFEE175 pKa = 4.47 TFLKK179 pKa = 10.8 RR180 pKa = 11.84 EE181 pKa = 4.24 FFRR184 pKa = 11.84 NLGDD188 pKa = 3.61 LTGLKK193 pKa = 8.83 VTGIDD198 pKa = 3.63 PAGLRR203 pKa = 11.84 PTPLPLVTSAAGSWVVPSGKK223 pKa = 9.94 YY224 pKa = 9.84 DD225 pKa = 3.58 LRR227 pKa = 11.84 VSSLGTAAPAAWNWVNGHH245 pKa = 5.95 WGEE248 pKa = 3.87 ALLRR252 pKa = 11.84 YY253 pKa = 9.3 SQAIGSWDD261 pKa = 3.69 TTNSWYY267 pKa = 10.74 RR268 pKa = 11.84 LVEE271 pKa = 3.72 EE272 pKa = 4.36 SARR275 pKa = 11.84 YY276 pKa = 9.42 SHH278 pKa = 6.99 FCSRR282 pKa = 11.84 MSRR285 pKa = 11.84 GRR287 pKa = 11.84 ISFKK291 pKa = 10.71 DD292 pKa = 3.76 EE293 pKa = 3.63 PAGKK297 pKa = 9.74 VRR299 pKa = 11.84 AFAMVDD305 pKa = 3.06 YY306 pKa = 7.77 WTQCSLKK313 pKa = 9.72 PLHH316 pKa = 7.13 DD317 pKa = 4.42 LLFSILKK324 pKa = 8.75 EE325 pKa = 3.96 IPQDD329 pKa = 3.53 GTFDD333 pKa = 3.29 QMAPAKK339 pKa = 9.66 EE340 pKa = 3.93 LLSVRR345 pKa = 11.84 RR346 pKa = 11.84 LATEE350 pKa = 4.13 TWWSLDD356 pKa = 3.19 LSAATDD362 pKa = 4.08 RR363 pKa = 11.84 FPLALQKK370 pKa = 11.0 LAMKK374 pKa = 10.04 YY375 pKa = 10.03 LVSEE379 pKa = 4.94 EE380 pKa = 4.36 YY381 pKa = 10.42 AAAWATLMVDD391 pKa = 3.68 RR392 pKa = 11.84 PFRR395 pKa = 11.84 LPVGYY400 pKa = 10.1 KK401 pKa = 10.25 PRR403 pKa = 11.84 DD404 pKa = 3.3 CRR406 pKa = 11.84 YY407 pKa = 10.46 AVGQPMGAYY416 pKa = 10.02 SSWAAFALTHH426 pKa = 6.24 HH427 pKa = 6.52 ATVQFAARR435 pKa = 11.84 LSGLSGWFRR444 pKa = 11.84 DD445 pKa = 3.91 YY446 pKa = 11.88 ALLGDD451 pKa = 5.49 DD452 pKa = 5.37 IIIANDD458 pKa = 3.46 RR459 pKa = 11.84 VAHH462 pKa = 6.7 KK463 pKa = 10.36 YY464 pKa = 9.75 RR465 pKa = 11.84 WLLSQLGVEE474 pKa = 4.48 CSLAKK479 pKa = 10.68 SMASNQRR486 pKa = 11.84 TFEE489 pKa = 3.79 FAKK492 pKa = 9.91 RR493 pKa = 11.84 VLFRR497 pKa = 11.84 GVDD500 pKa = 3.4 VSGFPWKK507 pKa = 10.3 LWRR510 pKa = 11.84 IGQRR514 pKa = 11.84 SLAAMISLAQRR525 pKa = 11.84 VSFGTAPLNLASLVKK540 pKa = 10.69 ALGAGMKK547 pKa = 8.64 ATSRR551 pKa = 11.84 AYY553 pKa = 8.52 TAWRR557 pKa = 11.84 HH558 pKa = 4.56 MPRR561 pKa = 11.84 GLKK564 pKa = 10.22 ALLIILTHH572 pKa = 5.99 PQANTFLSRR581 pKa = 11.84 QTWLDD586 pKa = 3.1 WLTAPGPVLPSGYY599 pKa = 10.4 KK600 pKa = 9.94 VDD602 pKa = 3.42 TSTWLTPWLSGFRR615 pKa = 11.84 EE616 pKa = 4.54 EE617 pKa = 4.64 YY618 pKa = 10.82 LNPALEE624 pKa = 4.1 FLEE627 pKa = 4.63 EE628 pKa = 4.31 EE629 pKa = 4.26 STLDD633 pKa = 4.6 LFEE636 pKa = 5.98 PDD638 pKa = 2.61 IDD640 pKa = 4.62 RR641 pKa = 11.84 RR642 pKa = 11.84 DD643 pKa = 3.56 AQGNLTRR650 pKa = 11.84 DD651 pKa = 3.32 SHH653 pKa = 7.64 YY654 pKa = 8.47 MAEE657 pKa = 4.97 FIRR660 pKa = 11.84 FRR662 pKa = 11.84 RR663 pKa = 11.84 AYY665 pKa = 10.04 LFSKK669 pKa = 10.74 LEE671 pKa = 3.99 SLVSVEE677 pKa = 3.7 RR678 pKa = 11.84 SKK680 pKa = 11.03 KK681 pKa = 9.26 IRR683 pKa = 11.84 QLEE686 pKa = 3.81 EE687 pKa = 3.26 RR688 pKa = 11.84 AAKK691 pKa = 10.15 IEE693 pKa = 4.08 ADD695 pKa = 3.55 LAHH698 pKa = 6.3 LQKK701 pKa = 11.08 LSISLQARR709 pKa = 11.84 NVSGMFNSMMKK720 pKa = 10.41 RR721 pKa = 11.84 LEE723 pKa = 3.92 EE724 pKa = 4.4 SIAAVPLPLPWLVWARR740 pKa = 11.84 EE741 pKa = 4.09 EE742 pKa = 4.4 NEE744 pKa = 3.65 NRR746 pKa = 11.84 RR747 pKa = 11.84 PATVLVSLWEE757 pKa = 3.88 RR758 pKa = 11.84 WRR760 pKa = 11.84 LRR762 pKa = 11.84 IHH764 pKa = 7.77 RR765 pKa = 11.84 SRR767 pKa = 11.84 KK768 pKa = 9.8 LPTHH772 pKa = 7.07 ANPQGEE778 pKa = 4.22 AVKK781 pKa = 10.79 ARR783 pKa = 11.84 FEE785 pKa = 4.65 KK786 pKa = 10.71 PFSTDD791 pKa = 3.09 FDD793 pKa = 4.06 EE794 pKa = 5.12
Molecular weight: 89.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.263
IPC2_protein 9.458
IPC_protein 9.765
Toseland 10.379
ProMoST 9.999
Dawson 10.511
Bjellqvist 10.175
Wikipedia 10.672
Rodwell 10.847
Grimsley 10.57
Solomon 10.555
Lehninger 10.54
Nozaki 10.365
DTASelect 10.16
Thurlkill 10.394
EMBOSS 10.774
Sillero 10.423
Patrickios 10.496
IPC_peptide 10.57
IPC2_peptide 8.887
IPC2.peptide.svr19 8.62
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
794
794
794
794.0
89.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.95 ± 0.0
0.756 ± 0.0
4.156 ± 0.0
4.66 ± 0.0
4.282 ± 0.0
5.416 ± 0.0
1.763 ± 0.0
4.156 ± 0.0
5.164 ± 0.0
12.469 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.267 ± 0.0
2.267 ± 0.0
5.416 ± 0.0
2.897 ± 0.0
9.068 ± 0.0
8.312 ± 0.0
4.912 ± 0.0
6.297 ± 0.0
3.526 ± 0.0
2.267 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here