Ulvibacter litoralis
Average proteome isoelectric point is 6.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3476 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G7CQV2|A0A1G7CQV2_9FLAO Uncharacterized protein OS=Ulvibacter litoralis OX=227084 GN=SAMN05421855_101507 PE=4 SV=1
MM1 pKa = 7.03 NRR3 pKa = 11.84 SVNYY7 pKa = 10.43 LMVVTAMTLALVTQSCSSDD26 pKa = 4.91 DD27 pKa = 4.26 DD28 pKa = 3.95 DD29 pKa = 4.76 TDD31 pKa = 3.26 IGNWTSRR38 pKa = 11.84 SIFDD42 pKa = 3.43 GAPRR46 pKa = 11.84 SSAASFVIDD55 pKa = 3.71 NIGYY59 pKa = 10.18 VGTGYY64 pKa = 10.92 DD65 pKa = 3.61 GDD67 pKa = 5.5 DD68 pKa = 3.61 YY69 pKa = 11.54 LTDD72 pKa = 3.89 FWAYY76 pKa = 10.62 DD77 pKa = 3.26 IDD79 pKa = 4.59 GDD81 pKa = 4.38 FWVQKK86 pKa = 11.05 ADD88 pKa = 3.63 FPGIARR94 pKa = 11.84 SSAVGFSVSNTGYY107 pKa = 10.37 IGTGYY112 pKa = 10.27 DD113 pKa = 3.11 AEE115 pKa = 4.57 AASEE119 pKa = 5.19 LNDD122 pKa = 3.5 FWAYY126 pKa = 10.69 SPSSNSWTQKK136 pKa = 10.74 ADD138 pKa = 3.67 FSGGNRR144 pKa = 11.84 RR145 pKa = 11.84 GSVAFAVGEE154 pKa = 3.94 KK155 pKa = 10.57 GYY157 pKa = 10.34 IGTGFDD163 pKa = 3.75 GDD165 pKa = 3.8 NDD167 pKa = 3.85 RR168 pKa = 11.84 KK169 pKa = 10.89 DD170 pKa = 3.2 FWEE173 pKa = 4.06 YY174 pKa = 11.5 SPTTDD179 pKa = 1.98 EE180 pKa = 3.84 WTEE183 pKa = 3.74 VFGYY187 pKa = 10.36 GGSKK191 pKa = 9.68 RR192 pKa = 11.84 RR193 pKa = 11.84 YY194 pKa = 7.67 ATTFTISDD202 pKa = 3.35 KK203 pKa = 10.9 VYY205 pKa = 10.97 LVTGVNNGLYY215 pKa = 10.28 VDD217 pKa = 5.02 DD218 pKa = 3.89 VWEE221 pKa = 4.44 FTPATGEE228 pKa = 4.06 WEE230 pKa = 4.34 KK231 pKa = 11.56 KK232 pKa = 10.23 NDD234 pKa = 3.39 LDD236 pKa = 4.43 YY237 pKa = 11.86 DD238 pKa = 3.56 EE239 pKa = 6.35 DD240 pKa = 5.08 YY241 pKa = 11.51 YY242 pKa = 10.3 ITRR245 pKa = 11.84 SNALGFSLNGLGYY258 pKa = 9.87 VSCGYY263 pKa = 10.4 SGGVLGSTWQYY274 pKa = 11.51 NSVSDD279 pKa = 3.38 TWEE282 pKa = 3.75 EE283 pKa = 3.93 LTALEE288 pKa = 3.99 ATARR292 pKa = 11.84 QDD294 pKa = 3.98 AISFTSTNNKK304 pKa = 9.74 AFVLLGRR311 pKa = 11.84 SGSLYY316 pKa = 10.82 LDD318 pKa = 3.49 DD319 pKa = 4.45 TYY321 pKa = 11.76 EE322 pKa = 4.07 FFPQEE327 pKa = 4.25 EE328 pKa = 4.56 LNEE331 pKa = 4.43 DD332 pKa = 3.84 DD333 pKa = 4.68
Molecular weight: 36.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.702
IPC2_protein 3.821
IPC_protein 3.846
Toseland 3.617
ProMoST 3.986
Dawson 3.846
Bjellqvist 4.012
Wikipedia 3.783
Rodwell 3.668
Grimsley 3.516
Solomon 3.834
Lehninger 3.795
Nozaki 3.948
DTASelect 4.215
Thurlkill 3.668
EMBOSS 3.795
Sillero 3.961
Patrickios 1.036
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.87
Protein with the highest isoelectric point:
>tr|A0A1G7F9G9|A0A1G7F9G9_9FLAO TIGR02757 family protein OS=Ulvibacter litoralis OX=227084 GN=SAMN05421855_102419 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSKK10 pKa = 9.13 RR11 pKa = 11.84 KK12 pKa = 9.48 RR13 pKa = 11.84 RR14 pKa = 11.84 NKK16 pKa = 9.49 HH17 pKa = 3.94 GFRR20 pKa = 11.84 EE21 pKa = 4.18 RR22 pKa = 11.84 MASVNGRR29 pKa = 11.84 KK30 pKa = 9.17 VLAARR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 9.87 GRR40 pKa = 11.84 KK41 pKa = 8.56 KK42 pKa = 10.21 ISVSSEE48 pKa = 3.74 TRR50 pKa = 11.84 HH51 pKa = 5.96 KK52 pKa = 10.68 KK53 pKa = 9.8
Molecular weight: 6.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.33
IPC2_protein 10.716
IPC_protein 12.266
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.281
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 12.003
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.051
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3476
0
3476
1153833
25
3179
331.9
37.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.683 ± 0.043
0.761 ± 0.014
5.509 ± 0.032
6.784 ± 0.038
5.182 ± 0.034
6.502 ± 0.04
1.712 ± 0.02
7.715 ± 0.041
7.308 ± 0.059
9.242 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.094 ± 0.019
5.952 ± 0.041
3.406 ± 0.024
3.31 ± 0.022
3.241 ± 0.027
6.706 ± 0.035
6.485 ± 0.05
6.438 ± 0.03
0.983 ± 0.015
3.988 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here