Lonchura maja polyomavirus 1
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1D8BJ51|A0A1D8BJ51_9POLY Putative ORF-X OS=Lonchura maja polyomavirus 1 OX=2169904 PE=4 SV=1
MM1 pKa = 7.27 STLQKK6 pKa = 10.89 LIDD9 pKa = 4.37 LLGLPPSATEE19 pKa = 3.5 ADD21 pKa = 3.51 VRR23 pKa = 11.84 SAYY26 pKa = 9.89 RR27 pKa = 11.84 KK28 pKa = 9.5 KK29 pKa = 10.66 ALEE32 pKa = 3.77 FHH34 pKa = 7.12 PDD36 pKa = 3.04 KK37 pKa = 11.5 GGDD40 pKa = 3.6 PEE42 pKa = 5.25 RR43 pKa = 11.84 MKK45 pKa = 10.73 EE46 pKa = 3.98 LNRR49 pKa = 11.84 LMDD52 pKa = 4.19 EE53 pKa = 4.43 FRR55 pKa = 11.84 HH56 pKa = 5.32 SQSLFCDD63 pKa = 3.41 EE64 pKa = 4.91 TLDD67 pKa = 5.14 SDD69 pKa = 4.44 TDD71 pKa = 3.99 DD72 pKa = 4.75 ADD74 pKa = 4.06 SPGPSQRR81 pKa = 11.84 TSTPEE86 pKa = 3.81 PGTGKK91 pKa = 10.39 DD92 pKa = 3.25 SGHH95 pKa = 5.77 GTFEE99 pKa = 4.29 PEE101 pKa = 3.84 VNSWHH106 pKa = 7.14 AIDD109 pKa = 5.22 YY110 pKa = 10.64 DD111 pKa = 3.62 RR112 pKa = 11.84 AYY114 pKa = 10.62 CKK116 pKa = 10.47 LMEE119 pKa = 5.06 LKK121 pKa = 9.68 WCLEE125 pKa = 4.24 TYY127 pKa = 7.69 FTSTEE132 pKa = 3.7 RR133 pKa = 11.84 RR134 pKa = 11.84 KK135 pKa = 10.21 QGLTPEE141 pKa = 3.97 YY142 pKa = 10.67 LEE144 pKa = 4.28 LKK146 pKa = 10.41 RR147 pKa = 11.84 RR148 pKa = 11.84 FQAVPWRR155 pKa = 11.84 VFDD158 pKa = 4.01 NVFNMDD164 pKa = 4.08 YY165 pKa = 10.73 II166 pKa = 4.95
Molecular weight: 19.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.967
IPC2_protein 5.003
IPC_protein 4.914
Toseland 4.813
ProMoST 5.054
Dawson 4.902
Bjellqvist 5.041
Wikipedia 4.787
Rodwell 4.8
Grimsley 4.736
Solomon 4.902
Lehninger 4.851
Nozaki 5.016
DTASelect 5.194
Thurlkill 4.825
EMBOSS 4.813
Sillero 5.08
Patrickios 4.113
IPC_peptide 4.902
IPC2_peptide 5.067
IPC2.peptide.svr19 5.067
Protein with the highest isoelectric point:
>tr|A0A1D8BJ44|A0A1D8BJ44_9POLY Minor capsid protein OS=Lonchura maja polyomavirus 1 OX=2169904 PE=3 SV=1
MM1 pKa = 7.56 ALAIWRR7 pKa = 11.84 EE8 pKa = 4.01 QIDD11 pKa = 3.45 ILFPGLSWLANNIHH25 pKa = 6.19 YY26 pKa = 10.41 LDD28 pKa = 4.3 PLHH31 pKa = 6.94 WARR34 pKa = 11.84 SLYY37 pKa = 9.69 NQVGRR42 pKa = 11.84 ALWNQIDD49 pKa = 3.6 QRR51 pKa = 11.84 VRR53 pKa = 11.84 DD54 pKa = 3.97 GLIGGAAQAAGAAGAEE70 pKa = 4.22 VAIRR74 pKa = 11.84 QSRR77 pKa = 11.84 TLYY80 pKa = 10.71 DD81 pKa = 3.31 VLARR85 pKa = 11.84 AMEE88 pKa = 4.09 VARR91 pKa = 11.84 WTVHH95 pKa = 5.32 SSYY98 pKa = 11.08 TSAEE102 pKa = 3.67 DD103 pKa = 3.36 TYY105 pKa = 11.53 RR106 pKa = 11.84 NLRR109 pKa = 11.84 EE110 pKa = 4.2 YY111 pKa = 10.53 YY112 pKa = 10.41 AQLPVDD118 pKa = 4.5 AGRR121 pKa = 11.84 PSYY124 pKa = 10.37 RR125 pKa = 11.84 RR126 pKa = 11.84 RR127 pKa = 11.84 LLGLTEE133 pKa = 3.94 QTSWDD138 pKa = 3.65 HH139 pKa = 6.24 GRR141 pKa = 11.84 GPTTSTSEE149 pKa = 3.82 PKK151 pKa = 10.77 AKK153 pKa = 10.54 VEE155 pKa = 4.22 EE156 pKa = 4.52 ASKK159 pKa = 10.58 QPPEE163 pKa = 4.38 SGEE166 pKa = 4.1 HH167 pKa = 5.02 VQDD170 pKa = 3.8 YY171 pKa = 8.43 PPPGGAHH178 pKa = 5.41 QRR180 pKa = 11.84 HH181 pKa = 5.67 APDD184 pKa = 3.0 WLLPLLLGLYY194 pKa = 10.8 GDD196 pKa = 4.56 ITPEE200 pKa = 3.37 WRR202 pKa = 11.84 SQLKK206 pKa = 8.51 QLKK209 pKa = 10.16 NGSQKK214 pKa = 10.4 RR215 pKa = 11.84 KK216 pKa = 8.94 RR217 pKa = 11.84 QLSPTPASPQADD229 pKa = 3.57 SKK231 pKa = 10.81 RR232 pKa = 11.84 RR233 pKa = 11.84 HH234 pKa = 5.69 RR235 pKa = 11.84 STRR238 pKa = 11.84 RR239 pKa = 11.84 KK240 pKa = 9.62 NRR242 pKa = 11.84 PP243 pKa = 2.95
Molecular weight: 27.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.258
IPC2_protein 9.502
IPC_protein 10.072
Toseland 10.028
ProMoST 9.867
Dawson 10.292
Bjellqvist 10.028
Wikipedia 10.526
Rodwell 10.452
Grimsley 10.394
Solomon 10.335
Lehninger 10.292
Nozaki 9.999
DTASelect 10.028
Thurlkill 10.116
EMBOSS 10.452
Sillero 10.204
Patrickios 9.663
IPC_peptide 10.321
IPC2_peptide 8.77
IPC2.peptide.svr19 8.685
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1937
166
612
322.8
35.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.673 ± 1.141
1.291 ± 0.399
5.731 ± 0.522
5.782 ± 0.439
2.426 ± 0.517
7.692 ± 0.919
2.375 ± 0.345
3.924 ± 0.399
4.285 ± 0.492
10.015 ± 0.67
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.962 ± 0.346
3.562 ± 0.39
7.692 ± 1.238
5.008 ± 0.335
6.557 ± 0.864
7.176 ± 0.764
6.557 ± 0.516
4.956 ± 0.522
1.291 ± 0.47
3.046 ± 0.489
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here