Prunus yedoensis var. nudiflora

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Rosales; Rosaceae; Amygdaloideae; Amygdaleae; Prunus; Prunus yedoensis

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 40375 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A314UL48|A0A314UL48_PRUYE Uncharacterized protein OS=Prunus yedoensis var. nudiflora OX=2094558 GN=Pyn_26608 PE=4 SV=1
MM1 pKa = 7.03SQANIDD7 pKa = 3.65YY8 pKa = 10.54GEE10 pKa = 4.35SFNEE14 pKa = 3.58IHH16 pKa = 7.85DD17 pKa = 4.42DD18 pKa = 3.71LFSQVDD24 pKa = 3.57EE25 pKa = 4.39NRR27 pKa = 11.84YY28 pKa = 9.76GSYY31 pKa = 11.03SLGDD35 pKa = 3.11DD36 pKa = 3.64HH37 pKa = 7.28LFQEE41 pKa = 4.73EE42 pKa = 4.3RR43 pKa = 11.84SALYY47 pKa = 10.28HH48 pKa = 6.82GEE50 pKa = 3.88PQDD53 pKa = 4.08FYY55 pKa = 11.9DD56 pKa = 5.37VLIHH60 pKa = 6.83TNDD63 pKa = 3.08EE64 pKa = 4.19YY65 pKa = 11.17EE66 pKa = 4.01YY67 pKa = 11.23DD68 pKa = 3.83GDD70 pKa = 5.21GDD72 pKa = 4.57DD73 pKa = 3.86KK74 pKa = 11.58TKK76 pKa = 10.89GFNSEE81 pKa = 4.26EE82 pKa = 4.11SDD84 pKa = 3.49NDD86 pKa = 3.72GNNSDD91 pKa = 4.2SEE93 pKa = 4.97FYY95 pKa = 11.15DD96 pKa = 3.66EE97 pKa = 6.51DD98 pKa = 4.03NDD100 pKa = 5.55AMLDD104 pKa = 3.64DD105 pKa = 5.8DD106 pKa = 5.34NFYY109 pKa = 11.54DD110 pKa = 4.89DD111 pKa = 6.29AVDD114 pKa = 3.53TLQAFIGCDD123 pKa = 3.34KK124 pKa = 10.8LHH126 pKa = 6.49SASNSDD132 pKa = 3.38EE133 pKa = 4.06EE134 pKa = 5.12GNTRR138 pKa = 11.84RR139 pKa = 11.84YY140 pKa = 10.45KK141 pKa = 10.52EE142 pKa = 4.02FNIDD146 pKa = 2.92TDD148 pKa = 3.23MDD150 pKa = 3.93NPQFDD155 pKa = 3.82VGMKK159 pKa = 10.29FPSCLLSARR168 pKa = 11.84KK169 pKa = 9.23VVSGGCMLLL178 pKa = 3.67

Molecular weight: 20.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A314XME3|A0A314XME3_PRUYE Uncharacterized protein OS=Prunus yedoensis var. nudiflora OX=2094558 GN=Pyn_41248 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 10.07GQPRR6 pKa = 11.84GARR9 pKa = 11.84ARR11 pKa = 11.84VGRR14 pKa = 11.84RR15 pKa = 11.84GRR17 pKa = 11.84GAVRR21 pKa = 11.84GRR23 pKa = 11.84GARR26 pKa = 11.84ARR28 pKa = 11.84GAATGGVDD36 pKa = 3.36TASANASGTTTASGTVGVRR55 pKa = 11.84GRR57 pKa = 11.84GVVRR61 pKa = 11.84GRR63 pKa = 11.84GVVSEE68 pKa = 4.08QQQTQARR75 pKa = 11.84PKK77 pKa = 9.92FNVKK81 pKa = 10.1RR82 pKa = 11.84GAHH85 pKa = 5.94APHH88 pKa = 7.3VIRR91 pKa = 11.84KK92 pKa = 6.91TNLFAGWQPGQGSSSQPPSQPTQNSQAPSQPAQSLQAHH130 pKa = 5.96QGSSSQPQPMVTSPKK145 pKa = 9.95RR146 pKa = 11.84PRR148 pKa = 11.84LKK150 pKa = 10.81SPAKK154 pKa = 10.22RR155 pKa = 11.84IRR157 pKa = 11.84PWRR160 pKa = 11.84VV161 pKa = 2.34

Molecular weight: 16.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

40375

0

40375

12929275

36

3511

320.2

35.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.815 ± 0.012

1.893 ± 0.007

5.195 ± 0.01

6.39 ± 0.014

4.19 ± 0.007

6.648 ± 0.014

2.431 ± 0.006

5.161 ± 0.008

6.035 ± 0.011

9.847 ± 0.016

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.459 ± 0.004

4.437 ± 0.009

4.98 ± 0.011

3.764 ± 0.008

5.264 ± 0.01

8.931 ± 0.013

4.915 ± 0.007

6.56 ± 0.009

1.335 ± 0.005

2.751 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski