Gordonia phage Horus
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 103 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A385DWH9|A0A385DWH9_9CAUD Membrane protein OS=Gordonia phage Horus OX=2301696 GN=27 PE=4 SV=1
MM1 pKa = 7.2 MNRR4 pKa = 11.84 RR5 pKa = 11.84 LAIAAVCAAAACILSSCSSDD25 pKa = 3.9 SGDD28 pKa = 3.21 TAAASSSSMAPSTVEE43 pKa = 3.81 TNGRR47 pKa = 11.84 GAIEE51 pKa = 4.15 VALGEE56 pKa = 4.22 PVAITGSDD64 pKa = 3.7 GAPVLTITGTRR75 pKa = 11.84 LDD77 pKa = 3.8 STGCAGDD84 pKa = 3.79 HH85 pKa = 7.05 PEE87 pKa = 3.94 VAHH90 pKa = 5.96 TKK92 pKa = 10.09 FVATIQTGDD101 pKa = 3.42 VEE103 pKa = 4.67 TEE105 pKa = 3.5 QWLWPSDD112 pKa = 3.5 FYY114 pKa = 11.89 YY115 pKa = 11.37 VNDD118 pKa = 4.05 ANKK121 pKa = 9.42 VAQNSEE127 pKa = 4.02 IEE129 pKa = 4.3 QATDD133 pKa = 2.9 TAFACEE139 pKa = 4.18 GSVEE143 pKa = 4.82 FIDD146 pKa = 4.98 VPPNTSKK153 pKa = 11.11 DD154 pKa = 3.52 GSPTLSVPVMTTAIGYY170 pKa = 9.22 HH171 pKa = 7.11 LDD173 pKa = 3.51 TAGVDD178 pKa = 4.07 QRR180 pKa = 11.84 VEE182 pKa = 3.79 WKK184 pKa = 10.76 LPAEE188 pKa = 4.22 WRR190 pKa = 11.84 QALAPVTSEE199 pKa = 4.27 APATTTEE206 pKa = 4.15 QPAPEE211 pKa = 4.28 PTNPAPEE218 pKa = 4.12 TTAPDD223 pKa = 3.35 TAAPDD228 pKa = 3.44 SGGIPPGWDD237 pKa = 2.97 KK238 pKa = 11.77 NGDD241 pKa = 3.45 GMIDD245 pKa = 3.22 TDD247 pKa = 4.84 APIGDD252 pKa = 4.12 DD253 pKa = 4.88 CEE255 pKa = 4.46 TPEE258 pKa = 4.42 CLMGEE263 pKa = 4.58 GGG265 pKa = 3.8
Molecular weight: 27.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.98
IPC2_protein 3.846
IPC_protein 3.834
Toseland 3.63
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.732
Rodwell 3.668
Grimsley 3.541
Solomon 3.808
Lehninger 3.77
Nozaki 3.923
DTASelect 4.139
Thurlkill 3.668
EMBOSS 3.745
Sillero 3.948
Patrickios 2.918
IPC_peptide 3.808
IPC2_peptide 3.935
IPC2.peptide.svr19 3.876
Protein with the highest isoelectric point:
>tr|A0A385DZ79|A0A385DZ79_9CAUD WhiB family transcription factor OS=Gordonia phage Horus OX=2301696 GN=70 PE=3 SV=1
MM1 pKa = 7.24 IGKK4 pKa = 8.92 PIRR7 pKa = 11.84 EE8 pKa = 4.13 LVATEE13 pKa = 4.62 DD14 pKa = 3.66 DD15 pKa = 3.88 PLYY18 pKa = 10.68 GLSARR23 pKa = 11.84 YY24 pKa = 9.22 VRR26 pKa = 11.84 RR27 pKa = 11.84 HH28 pKa = 5.75 LEE30 pKa = 3.78 AGGLFRR36 pKa = 11.84 GLGSQRR42 pKa = 11.84 CKK44 pKa = 10.29 RR45 pKa = 11.84 GEE47 pKa = 3.73 WLLNRR52 pKa = 11.84 EE53 pKa = 4.45 DD54 pKa = 3.27 VAEE57 pKa = 3.93 IQRR60 pKa = 11.84 RR61 pKa = 11.84 LRR63 pKa = 11.84 AVPDD67 pKa = 3.6 QEE69 pKa = 4.38 PVEE72 pKa = 4.59 LDD74 pKa = 3.6 TPSGLSSRR82 pKa = 11.84 SRR84 pKa = 11.84 TLRR87 pKa = 11.84 RR88 pKa = 11.84 IQQGRR93 pKa = 11.84 ASSS96 pKa = 3.28
Molecular weight: 10.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.335
IPC2_protein 9.355
IPC_protein 10.423
Toseland 10.379
ProMoST 10.248
Dawson 10.526
Bjellqvist 10.321
Wikipedia 10.804
Rodwell 10.482
Grimsley 10.599
Solomon 10.701
Lehninger 10.657
Nozaki 10.394
DTASelect 10.321
Thurlkill 10.423
EMBOSS 10.818
Sillero 10.467
Patrickios 10.277
IPC_peptide 10.701
IPC2_peptide 9.516
IPC2.peptide.svr19 8.546
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
103
0
103
17642
28
1862
171.3
18.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.24 ± 0.425
1.196 ± 0.194
6.502 ± 0.303
5.617 ± 0.29
2.726 ± 0.219
8.446 ± 0.35
2.211 ± 0.168
4.654 ± 0.187
3.656 ± 0.275
7.777 ± 0.288
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.369 ± 0.176
2.766 ± 0.188
5.391 ± 0.195
3.514 ± 0.157
7.176 ± 0.336
5.901 ± 0.266
6.485 ± 0.308
7.737 ± 0.356
2.205 ± 0.131
2.432 ± 0.194
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here