Streptococcus phage CHPC1230

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G8FBL4|A0A3G8FBL4_9CAUD Uncharacterized protein OS=Streptococcus phage CHPC1230 OX=2365031 GN=CHPC1230_0054 PE=4 SV=1
MM1 pKa = 7.11VNWVDD6 pKa = 4.56ADD8 pKa = 4.01GNDD11 pKa = 3.57IPDD14 pKa = 4.12GADD17 pKa = 3.05QEE19 pKa = 5.1FKK21 pKa = 11.28AGMFFSFAGDD31 pKa = 3.23EE32 pKa = 4.35TNIVDD37 pKa = 3.97TGNGGYY43 pKa = 10.4YY44 pKa = 9.73GGYY47 pKa = 9.69YY48 pKa = 9.72YY49 pKa = 10.64RR50 pKa = 11.84QFEE53 pKa = 4.2FGQFGTVWLSCWSKK67 pKa = 11.71DD68 pKa = 3.97DD69 pKa = 4.56LVNYY73 pKa = 7.43YY74 pKa = 8.26QQ75 pKa = 4.22

Molecular weight: 8.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G8FBG2|A0A3G8FBG2_9CAUD Terminase large subunit OS=Streptococcus phage CHPC1230 OX=2365031 GN=CHPC1230_0004 PE=4 SV=1
MM1 pKa = 7.83ADD3 pKa = 3.75FGVSLLEE10 pKa = 4.01ARR12 pKa = 11.84RR13 pKa = 11.84MTLKK17 pKa = 10.49EE18 pKa = 3.53MKK20 pKa = 9.92LYY22 pKa = 10.32QKK24 pKa = 10.55AYY26 pKa = 9.84KK27 pKa = 10.03KK28 pKa = 10.52RR29 pKa = 11.84FLNKK33 pKa = 9.43EE34 pKa = 3.69RR35 pKa = 11.84EE36 pKa = 4.36IYY38 pKa = 9.68QLAYY42 pKa = 10.87LNRR45 pKa = 11.84LANATTKK52 pKa = 10.62DD53 pKa = 3.03GKK55 pKa = 10.64KK56 pKa = 10.46YY57 pKa = 10.49YY58 pKa = 10.19FEE60 pKa = 5.48KK61 pKa = 10.83FDD63 pKa = 3.95DD64 pKa = 4.49FYY66 pKa = 11.56NAKK69 pKa = 9.54EE70 pKa = 3.83RR71 pKa = 11.84ARR73 pKa = 11.84EE74 pKa = 3.84VLGEE78 pKa = 4.35KK79 pKa = 8.61ITNSKK84 pKa = 10.18LLEE87 pKa = 3.98RR88 pKa = 11.84ARR90 pKa = 11.84NNLNYY95 pKa = 10.06KK96 pKa = 9.85KK97 pKa = 10.46EE98 pKa = 3.94RR99 pKa = 11.84GLLDD103 pKa = 3.22GRR105 pKa = 4.46

Molecular weight: 12.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

11860

44

1545

215.6

24.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.395 ± 1.146

0.624 ± 0.146

6.088 ± 0.475

7.04 ± 0.75

4.03 ± 0.179

6.669 ± 0.538

1.4 ± 0.221

6.703 ± 0.334

8.314 ± 0.661

7.605 ± 0.306

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.681 ± 0.301

5.556 ± 0.38

2.715 ± 0.233

4.106 ± 0.301

4.418 ± 0.425

6.737 ± 0.56

6.298 ± 0.416

6.391 ± 0.295

1.298 ± 0.191

3.929 ± 0.482
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski