Acetilactobacillus jinshanensis
Average proteome isoelectric point is 8.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1450 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P6ZLK8|A0A4P6ZLK8_9LACO Glutamine synthetase OS=Acetilactobacillus jinshanensis OX=1720083 GN=glnA PE=3 SV=1
MM1 pKa = 7.71 EE2 pKa = 5.44 SLNSNQQISLTDD14 pKa = 3.35 KK15 pKa = 11.03 DD16 pKa = 4.19 GNTVVYY22 pKa = 10.65 NVLLTFKK29 pKa = 10.09 STDD32 pKa = 3.7 FHH34 pKa = 7.77 KK35 pKa = 10.62 SYY37 pKa = 11.11 ILIYY41 pKa = 10.07 PDD43 pKa = 3.87 GRR45 pKa = 11.84 PEE47 pKa = 3.83 NQQVGVRR54 pKa = 11.84 AFALPPDD61 pKa = 4.48 EE62 pKa = 6.21 DD63 pKa = 3.91 PANPQSGDD71 pKa = 3.36 LMPIEE76 pKa = 4.42 TQKK79 pKa = 10.54 EE80 pKa = 3.64 WDD82 pKa = 3.58 MVEE85 pKa = 4.4 SVLNTFINPNEE96 pKa = 3.88
Molecular weight: 10.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.32
IPC2_protein 4.164
IPC_protein 4.062
Toseland 3.872
ProMoST 4.151
Dawson 4.037
Bjellqvist 4.266
Wikipedia 3.961
Rodwell 3.897
Grimsley 3.795
Solomon 4.024
Lehninger 3.986
Nozaki 4.164
DTASelect 4.342
Thurlkill 3.923
EMBOSS 3.973
Sillero 4.177
Patrickios 3.719
IPC_peptide 4.024
IPC2_peptide 4.164
IPC2.peptide.svr19 4.097
Protein with the highest isoelectric point:
>tr|A0A4P6ZJS9|A0A4P6ZJS9_9LACO Pyruvate kinase OS=Acetilactobacillus jinshanensis OX=1720083 GN=pyk PE=3 SV=1
MM1 pKa = 7.29 VKK3 pKa = 10.27 RR4 pKa = 11.84 PVSNNIKK11 pKa = 10.59 CPFCGTTFYY20 pKa = 10.88 RR21 pKa = 11.84 SDD23 pKa = 3.26 LSYY26 pKa = 11.24 VDD28 pKa = 3.0 SRR30 pKa = 11.84 VYY32 pKa = 10.29 LKK34 pKa = 10.77 RR35 pKa = 11.84 MKK37 pKa = 10.39 NRR39 pKa = 11.84 LEE41 pKa = 3.98 IYY43 pKa = 10.24 PLGKK47 pKa = 10.0 NVAASPKK54 pKa = 10.02 LKK56 pKa = 9.75 QQHH59 pKa = 6.58 PEE61 pKa = 3.79 LLRR64 pKa = 11.84 LEE66 pKa = 4.52 MSACPNCHH74 pKa = 6.95 RR75 pKa = 11.84 ITVRR79 pKa = 11.84 AVGIGDD85 pKa = 3.63 QYY87 pKa = 10.79 KK88 pKa = 9.04 HH89 pKa = 5.79 HH90 pKa = 7.0 HH91 pKa = 6.23 VFNIYY96 pKa = 10.03 PRR98 pKa = 11.84 FNADD102 pKa = 3.51 PLPTYY107 pKa = 10.83 VPDD110 pKa = 5.48 QIRR113 pKa = 11.84 QDD115 pKa = 3.46 YY116 pKa = 10.65 KK117 pKa = 10.58 EE118 pKa = 4.13 ANEE121 pKa = 4.25 VYY123 pKa = 9.93 EE124 pKa = 4.05 VSPNSAAMLARR135 pKa = 11.84 RR136 pKa = 11.84 ILEE139 pKa = 4.1 EE140 pKa = 4.79 IIADD144 pKa = 4.73 FYY146 pKa = 11.26 HH147 pKa = 8.03 IEE149 pKa = 4.19 EE150 pKa = 4.86 GDD152 pKa = 3.87 LYY154 pKa = 11.25 HH155 pKa = 7.78 DD156 pKa = 5.41 LDD158 pKa = 3.97 QLRR161 pKa = 11.84 HH162 pKa = 5.39 KK163 pKa = 10.57 QSNPRR168 pKa = 11.84 VWQAIDD174 pKa = 3.43 SIRR177 pKa = 11.84 RR178 pKa = 11.84 LGNIGAHH185 pKa = 4.36 QTQNVDD191 pKa = 3.18 NVFGNVSVKK200 pKa = 9.79 GAKK203 pKa = 9.77 EE204 pKa = 3.73 IIGLIRR210 pKa = 11.84 MVIHH214 pKa = 6.3 DD215 pKa = 4.7 TYY217 pKa = 11.77 VEE219 pKa = 4.16 DD220 pKa = 3.42 HH221 pKa = 6.99 LSRR224 pKa = 11.84 HH225 pKa = 5.49 LEE227 pKa = 3.81 KK228 pKa = 10.94 SVIRR232 pKa = 11.84 SAKK235 pKa = 8.54 KK236 pKa = 10.33 LNYY239 pKa = 8.67 QPHH242 pKa = 6.82 YY243 pKa = 9.9 YY244 pKa = 9.93 PSDD247 pKa = 3.66 EE248 pKa = 4.3 NSQSSRR254 pKa = 11.84 RR255 pKa = 11.84 NYY257 pKa = 10.17 RR258 pKa = 11.84 SRR260 pKa = 11.84 NTHH263 pKa = 5.84 KK264 pKa = 10.61 SYY266 pKa = 11.24 SSHH269 pKa = 6.25 NSNNANRR276 pKa = 11.84 SKK278 pKa = 10.89 SSNASRR284 pKa = 11.84 GHH286 pKa = 4.22 QQHH289 pKa = 6.66 RR290 pKa = 11.84 NNHH293 pKa = 5.31 RR294 pKa = 11.84 RR295 pKa = 11.84 NQNNSRR301 pKa = 11.84 RR302 pKa = 11.84 EE303 pKa = 3.78 NNRR306 pKa = 11.84 RR307 pKa = 11.84 SNQKK311 pKa = 10.1 RR312 pKa = 11.84 NIRR315 pKa = 11.84 RR316 pKa = 11.84 NSSTPKK322 pKa = 9.02 RR323 pKa = 11.84 NNYY326 pKa = 9.07 RR327 pKa = 11.84 KK328 pKa = 9.57 QSRR331 pKa = 11.84 PSNNRR336 pKa = 11.84 KK337 pKa = 9.61 SNQRR341 pKa = 11.84 NSNSYY346 pKa = 10.75 RR347 pKa = 11.84 KK348 pKa = 6.69 THH350 pKa = 6.56 RR351 pKa = 11.84 NNNYY355 pKa = 9.21 RR356 pKa = 11.84 RR357 pKa = 11.84 STRR360 pKa = 11.84 SRR362 pKa = 11.84 EE363 pKa = 3.78 RR364 pKa = 11.84 NQQSRR369 pKa = 11.84 SRR371 pKa = 11.84 HH372 pKa = 3.77 TQRR375 pKa = 11.84 RR376 pKa = 11.84 STRR379 pKa = 11.84 HH380 pKa = 4.62 SKK382 pKa = 10.32 KK383 pKa = 10.29 SRR385 pKa = 11.84 VRR387 pKa = 11.84 DD388 pKa = 3.4 KK389 pKa = 11.26 SFTIIQGKK397 pKa = 9.02
Molecular weight: 46.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.322
IPC2_protein 9.999
IPC_protein 10.95
Toseland 10.935
ProMoST 10.833
Dawson 11.023
Bjellqvist 10.804
Wikipedia 11.301
Rodwell 11.14
Grimsley 11.082
Solomon 11.213
Lehninger 11.169
Nozaki 10.921
DTASelect 10.804
Thurlkill 10.935
EMBOSS 11.359
Sillero 10.965
Patrickios 10.818
IPC_peptide 11.213
IPC2_peptide 9.926
IPC2.peptide.svr19 8.333
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1450
0
1450
454499
39
6060
313.4
35.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.043 ± 0.159
0.676 ± 0.02
5.296 ± 0.06
3.411 ± 0.043
4.105 ± 0.062
6.306 ± 0.059
3.34 ± 0.043
7.507 ± 0.089
8.072 ± 0.099
8.995 ± 0.107
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.859 ± 0.045
5.988 ± 0.094
3.901 ± 0.048
4.162 ± 0.051
4.313 ± 0.079
6.681 ± 0.233
5.45 ± 0.06
6.774 ± 0.056
1.034 ± 0.021
4.086 ± 0.048
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here