Streptococcus satellite phage Javan730
Average proteome isoelectric point is 7.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 25 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZV43|A0A4D5ZV43_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan730 OX=2558824 GN=JavanS730_0014 PE=4 SV=1
MM1 pKa = 7.44 EE2 pKa = 5.43 PKK4 pKa = 10.1 IKK6 pKa = 10.9 NGDD9 pKa = 3.4 YY10 pKa = 11.13 LFIKK14 pKa = 8.35 NTPQVDD20 pKa = 3.97 YY21 pKa = 8.82 NTIGIFQVDD30 pKa = 3.58 GANYY34 pKa = 9.54 VKK36 pKa = 10.81 KK37 pKa = 10.47 LRR39 pKa = 11.84 QGYY42 pKa = 9.65 LEE44 pKa = 4.73 SLNPDD49 pKa = 3.66 CADD52 pKa = 3.23 IQLDD56 pKa = 3.78 EE57 pKa = 5.74 DD58 pKa = 3.79 NDD60 pKa = 3.21 IRR62 pKa = 11.84 TIGEE66 pKa = 3.96 VVSIYY71 pKa = 10.62 RR72 pKa = 11.84 EE73 pKa = 3.88 NN74 pKa = 3.2
Molecular weight: 8.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.404
IPC2_protein 4.444
IPC_protein 4.304
Toseland 4.113
ProMoST 4.444
Dawson 4.279
Bjellqvist 4.431
Wikipedia 4.202
Rodwell 4.139
Grimsley 4.024
Solomon 4.266
Lehninger 4.228
Nozaki 4.393
DTASelect 4.609
Thurlkill 4.151
EMBOSS 4.215
Sillero 4.418
Patrickios 3.63
IPC_peptide 4.266
IPC2_peptide 4.406
IPC2.peptide.svr19 4.355
Protein with the highest isoelectric point:
>tr|A0A4D5ZU69|A0A4D5ZU69_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan730 OX=2558824 GN=JavanS730_0011 PE=4 SV=1
MM1 pKa = 7.87 KK2 pKa = 9.27 ITEE5 pKa = 4.16 YY6 pKa = 10.16 TKK8 pKa = 10.88 KK9 pKa = 10.64 DD10 pKa = 2.96 GTTVYY15 pKa = 10.26 RR16 pKa = 11.84 SSVYY20 pKa = 10.68 LGIDD24 pKa = 3.15 TVTGKK29 pKa = 10.24 KK30 pKa = 10.27 VKK32 pKa = 8.07 TTISGRR38 pKa = 11.84 TKK40 pKa = 10.61 RR41 pKa = 11.84 EE42 pKa = 3.76 LKK44 pKa = 10.7 AKK46 pKa = 10.16 ALQAQIDD53 pKa = 4.1 FEE55 pKa = 5.07 KK56 pKa = 11.04 GGSTVYY62 pKa = 10.52 KK63 pKa = 10.06 AVEE66 pKa = 3.74 IKK68 pKa = 9.8 TYY70 pKa = 11.23 AEE72 pKa = 4.37 LVEE75 pKa = 4.62 NWLEE79 pKa = 4.57 TYY81 pKa = 10.53 CHH83 pKa = 5.45 TVKK86 pKa = 10.81 KK87 pKa = 9.8 ATLTIIKK94 pKa = 10.16 SRR96 pKa = 11.84 LNNYY100 pKa = 8.32 LLPAFGEE107 pKa = 4.31 YY108 pKa = 10.55 KK109 pKa = 10.15 LDD111 pKa = 3.64 KK112 pKa = 9.63 LTPPVIQKK120 pKa = 9.14 QVNQWAKK127 pKa = 10.6 EE128 pKa = 3.84 YY129 pKa = 9.72 NQLGKK134 pKa = 10.69 GYY136 pKa = 9.89 QEE138 pKa = 3.99 YY139 pKa = 9.14 PQLNSLNKK147 pKa = 10.15 RR148 pKa = 11.84 ILKK151 pKa = 9.76 YY152 pKa = 10.38 AVSLQVIPFNPARR165 pKa = 11.84 DD166 pKa = 3.53 IIVPRR171 pKa = 11.84 RR172 pKa = 11.84 KK173 pKa = 9.74 EE174 pKa = 3.8 KK175 pKa = 10.62 EE176 pKa = 4.12 GQKK179 pKa = 10.54 LKK181 pKa = 11.2 YY182 pKa = 10.43 LDD184 pKa = 4.8 DD185 pKa = 5.74 DD186 pKa = 4.0 NLKK189 pKa = 10.77 KK190 pKa = 10.58 FLTYY194 pKa = 10.66 LEE196 pKa = 4.38 QLPNTYY202 pKa = 10.74 KK203 pKa = 10.78 NFYY206 pKa = 9.48 DD207 pKa = 3.51 TVLYY211 pKa = 8.15 KK212 pKa = 10.45 TLLATGLRR220 pKa = 11.84 IRR222 pKa = 11.84 EE223 pKa = 4.08 CLALKK228 pKa = 10.05 WSDD231 pKa = 3.08 IDD233 pKa = 4.02 LQNGTLDD240 pKa = 3.26 VSKK243 pKa = 9.48 TLNCEE248 pKa = 4.16 KK249 pKa = 10.47 EE250 pKa = 4.29 VTSPKK255 pKa = 8.61 TKK257 pKa = 10.49 SSIRR261 pKa = 11.84 VIDD264 pKa = 4.28 LDD266 pKa = 3.81 NKK268 pKa = 8.09 TVLMLRR274 pKa = 11.84 LYY276 pKa = 10.7 KK277 pKa = 10.34 NRR279 pKa = 11.84 QAQVGRR285 pKa = 11.84 EE286 pKa = 3.53 IGLTYY291 pKa = 10.68 EE292 pKa = 4.41 KK293 pKa = 10.78 VFSNSFDD300 pKa = 3.44 EE301 pKa = 4.33 YY302 pKa = 10.78 RR303 pKa = 11.84 DD304 pKa = 3.44 ARR306 pKa = 11.84 ALRR309 pKa = 11.84 SRR311 pKa = 11.84 LEE313 pKa = 3.85 KK314 pKa = 10.32 HH315 pKa = 6.43 LKK317 pKa = 10.66 LSDD320 pKa = 3.61 CPRR323 pKa = 11.84 LTFHH327 pKa = 7.8 AFRR330 pKa = 11.84 HH331 pKa = 4.54 THH333 pKa = 6.71 ASILLNAGLPYY344 pKa = 10.43 KK345 pKa = 10.2 EE346 pKa = 3.68 IQTRR350 pKa = 11.84 LGHH353 pKa = 6.71 AKK355 pKa = 10.54 LSMTMDD361 pKa = 3.71 IYY363 pKa = 11.79 SHH365 pKa = 7.09 LSKK368 pKa = 10.88 DD369 pKa = 3.45 NKK371 pKa = 10.49 KK372 pKa = 10.17 NATSFYY378 pKa = 9.85 EE379 pKa = 3.99 KK380 pKa = 10.33 AIEE383 pKa = 4.04 KK384 pKa = 10.35 LKK386 pKa = 11.16 SSS388 pKa = 3.82
Molecular weight: 44.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.106
IPC2_protein 9.121
IPC_protein 8.99
Toseland 9.78
ProMoST 9.472
Dawson 9.999
Bjellqvist 9.663
Wikipedia 10.16
Rodwell 10.452
Grimsley 10.072
Solomon 10.014
Lehninger 9.984
Nozaki 9.78
DTASelect 9.663
Thurlkill 9.853
EMBOSS 10.189
Sillero 9.911
Patrickios 7.6
IPC_peptide 10.014
IPC2_peptide 8.17
IPC2.peptide.svr19 8.213
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
25
0
25
3516
49
388
140.6
16.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.977 ± 0.399
0.91 ± 0.109
5.944 ± 0.533
8.874 ± 0.606
4.38 ± 0.448
5.034 ± 0.428
1.451 ± 0.208
6.883 ± 0.424
11.234 ± 0.485
10.04 ± 0.619
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.389 ± 0.272
5.319 ± 0.346
2.418 ± 0.248
3.641 ± 0.31
4.949 ± 0.407
5.717 ± 0.546
5.944 ± 0.543
4.892 ± 0.332
0.768 ± 0.144
4.238 ± 0.304
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here