Aurantimonas sp. 22II-16-19i
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5291 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1X0SV04|A0A1X0SV04_9RHIZ Translation initiation factor IF-2 (Fragment) OS=Aurantimonas sp. 22II-16-19i OX=1317114 GN=infB PE=4 SV=1
MM1 pKa = 7.3 VFNIHH6 pKa = 6.31 YY7 pKa = 10.41 DD8 pKa = 3.34 VAAFNNLAGLLSIQVLEE25 pKa = 4.58 PEE27 pKa = 4.37 NNADD31 pKa = 3.56 SGLNGLRR38 pKa = 11.84 GSAAFNFFNEE48 pKa = 4.16 LGVPIEE54 pKa = 4.93 DD55 pKa = 4.3 GPLGALGIFVEE66 pKa = 5.06 GPGDD70 pKa = 3.59 IDD72 pKa = 5.45 DD73 pKa = 4.95 PATDD77 pKa = 3.96 VYY79 pKa = 9.65 HH80 pKa = 5.14 TTYY83 pKa = 11.25 AHH85 pKa = 4.52 VHH87 pKa = 4.88 GVEE90 pKa = 4.31 EE91 pKa = 4.42 NLTDD95 pKa = 3.75 TPLGTNTFDD104 pKa = 3.23 SGLAITPGEE113 pKa = 4.01 YY114 pKa = 10.23 LAPNVITVNGRR125 pKa = 11.84 IPTFATGEE133 pKa = 3.86 LWQPVTLHH141 pKa = 5.16 QRR143 pKa = 11.84 QVIDD147 pKa = 3.34 KK148 pKa = 10.4 DD149 pKa = 3.91 DD150 pKa = 3.66 YY151 pKa = 10.22 FTINFYY157 pKa = 10.92 VKK159 pKa = 9.94 PGAVQQVPGQKK170 pKa = 9.49 PVASANFSTSYY181 pKa = 11.07 SLSTDD186 pKa = 3.46 ALPHH190 pKa = 5.15 YY191 pKa = 10.58 AFYY194 pKa = 11.54 ANMFDD199 pKa = 4.22 PGVGLNSLGDD209 pKa = 3.29 ILFYY213 pKa = 10.9 SEE215 pKa = 4.54 NSIQIGYY222 pKa = 10.47 DD223 pKa = 3.39 DD224 pKa = 5.2 GDD226 pKa = 3.45 QGNYY230 pKa = 9.08 TIYY233 pKa = 10.43 TGDD236 pKa = 3.42 FKK238 pKa = 11.43 FEE240 pKa = 4.13 EE241 pKa = 4.78 DD242 pKa = 3.46 ASTGNVFLVEE252 pKa = 4.11 GFISQIDD259 pKa = 3.6 HH260 pKa = 5.84 QYY262 pKa = 10.05 YY263 pKa = 8.03 YY264 pKa = 10.43 HH265 pKa = 7.84 DD266 pKa = 3.93 YY267 pKa = 10.43 SVTPEE272 pKa = 3.93 VYY274 pKa = 10.73 YY275 pKa = 11.01 SGLWTASSVGITAAEE290 pKa = 3.85 ARR292 pKa = 11.84 DD293 pKa = 4.11 LFGQDD298 pKa = 2.83 FSEE301 pKa = 5.27 RR302 pKa = 11.84 IFRR305 pKa = 11.84 GNDD308 pKa = 3.06 TLKK311 pKa = 11.02 GSTEE315 pKa = 3.95 ADD317 pKa = 3.24 RR318 pKa = 11.84 LFAFAGNDD326 pKa = 3.27 ILIGGAGADD335 pKa = 3.63 QLNGGTGSDD344 pKa = 3.31 TASYY348 pKa = 10.59 AGSLSVRR355 pKa = 11.84 VNLTNGRR362 pKa = 11.84 GSFGDD367 pKa = 3.5 AEE369 pKa = 4.65 GDD371 pKa = 3.67 VLIGIEE377 pKa = 4.42 NITGSEE383 pKa = 4.05 RR384 pKa = 11.84 ADD386 pKa = 3.41 TLLGNSGANVINGEE400 pKa = 4.07 GGFDD404 pKa = 4.1 EE405 pKa = 4.12 IHH407 pKa = 6.83 GYY409 pKa = 10.2 AGNDD413 pKa = 2.96 RR414 pKa = 11.84 LYY416 pKa = 11.2 GGNGLDD422 pKa = 4.45 KK423 pKa = 11.08 LYY425 pKa = 11.39 GDD427 pKa = 5.4 FNADD431 pKa = 3.41 MLFGGAGGDD440 pKa = 3.88 TLSGGAGPDD449 pKa = 3.14 KK450 pKa = 11.25 VYY452 pKa = 11.27 GEE454 pKa = 4.64 SGNDD458 pKa = 3.63 NIVGDD463 pKa = 4.26 ADD465 pKa = 3.72 GSEE468 pKa = 4.02 DD469 pKa = 3.96 VYY471 pKa = 11.77 DD472 pKa = 4.59 GGTEE476 pKa = 3.88 IDD478 pKa = 3.72 TVDD481 pKa = 3.52 YY482 pKa = 10.7 GAVASAINVNLSLNFAMGAPIGTDD506 pKa = 2.79 RR507 pKa = 11.84 IFAVEE512 pKa = 4.87 RR513 pKa = 11.84 ILAGAGNDD521 pKa = 3.61 KK522 pKa = 9.95 LTGNAFTTLLSGAGGNDD539 pKa = 3.5 VIVGGIGDD547 pKa = 3.74 NKK549 pKa = 10.68 LYY551 pKa = 11.11 GGDD554 pKa = 3.19 GHH556 pKa = 7.52 DD557 pKa = 4.11 NLRR560 pKa = 11.84 GLAGFDD566 pKa = 3.2 QFFGGNGNDD575 pKa = 3.55 RR576 pKa = 11.84 MQGDD580 pKa = 4.49 FNADD584 pKa = 3.12 TFIFANGFGRR594 pKa = 11.84 DD595 pKa = 3.5 VIADD599 pKa = 4.12 FEE601 pKa = 4.48 ALNAAEE607 pKa = 4.93 QIDD610 pKa = 4.35 LSGVTNITSFADD622 pKa = 3.75 LAANHH627 pKa = 6.87 LSQVGGNAVITDD639 pKa = 4.21 GFNTITLNGVAIGDD653 pKa = 4.18 LDD655 pKa = 3.57 ATDD658 pKa = 5.74 FIFF661 pKa = 5.37
Molecular weight: 69.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.681
IPC2_protein 3.77
IPC_protein 3.821
Toseland 3.592
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.77
Rodwell 3.643
Grimsley 3.49
Solomon 3.821
Lehninger 3.783
Nozaki 3.923
DTASelect 4.202
Thurlkill 3.643
EMBOSS 3.77
Sillero 3.948
Patrickios 1.494
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.837
Protein with the highest isoelectric point:
>tr|A0A1X0T213|A0A1X0T213_9RHIZ Putative major facilitator superfamily MFS-1 OS=Aurantimonas sp. 22II-16-19i OX=1317114 GN=ATO4_13590 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.4 RR3 pKa = 11.84 TYY5 pKa = 10.2 QPSRR9 pKa = 11.84 LVRR12 pKa = 11.84 KK13 pKa = 9.21 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.48 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.33 SGQKK29 pKa = 9.93 VIAARR34 pKa = 11.84 RR35 pKa = 11.84 SRR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.96 RR41 pKa = 11.84 LSAA44 pKa = 4.03
Molecular weight: 5.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5291
0
5291
1651721
17
3005
312.2
33.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.287 ± 0.06
0.788 ± 0.01
5.905 ± 0.032
6.004 ± 0.031
3.752 ± 0.025
9.037 ± 0.048
1.86 ± 0.017
5.045 ± 0.028
2.89 ± 0.029
9.847 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.433 ± 0.014
2.295 ± 0.021
5.204 ± 0.032
2.791 ± 0.019
7.355 ± 0.038
5.429 ± 0.023
5.315 ± 0.025
7.475 ± 0.029
1.205 ± 0.014
2.084 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here