Geotoga petraea

Taxonomy: cellular organisms; Bacteria; Thermotogae; Thermotogae; Petrotogales; Petrotogaceae; Geotoga

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2070 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G6PLP1|A0A1G6PLP1_9BACT Anti-sigma factor antagonist OS=Geotoga petraea OX=28234 GN=E4650_06525 PE=3 SV=1
MM1 pKa = 7.49KK2 pKa = 10.1KK3 pKa = 9.89YY4 pKa = 10.46RR5 pKa = 11.84CIICGYY11 pKa = 10.02IYY13 pKa = 10.51DD14 pKa = 4.75PEE16 pKa = 4.73QGDD19 pKa = 3.77PDD21 pKa = 3.93NGVEE25 pKa = 4.43PGTSFDD31 pKa = 5.32DD32 pKa = 4.87LPEE35 pKa = 4.73DD36 pKa = 3.95WVCPICGASKK46 pKa = 10.68EE47 pKa = 4.09DD48 pKa = 3.86FEE50 pKa = 5.17VFEE53 pKa = 4.57

Molecular weight: 6.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G6M7H6|A0A1G6M7H6_9BACT FeoC domain-containing protein OS=Geotoga petraea OX=28234 GN=E4650_06995 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.16QPSRR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 9.66RR12 pKa = 11.84KK13 pKa = 7.81RR14 pKa = 11.84THH16 pKa = 5.82GFLVRR21 pKa = 11.84KK22 pKa = 7.55RR23 pKa = 11.84TSGGKK28 pKa = 8.85NVLRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.51GRR39 pKa = 11.84KK40 pKa = 8.63RR41 pKa = 11.84LSVV44 pKa = 3.2

Molecular weight: 5.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2070

0

2070

660910

30

1751

319.3

36.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.592 ± 0.049

0.455 ± 0.014

5.898 ± 0.038

8.008 ± 0.061

5.42 ± 0.049

5.766 ± 0.05

1.358 ± 0.021

10.163 ± 0.062

9.726 ± 0.086

8.985 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.481 ± 0.026

6.794 ± 0.057

2.882 ± 0.028

2.286 ± 0.027

3.115 ± 0.031

6.369 ± 0.053

4.641 ± 0.037

5.732 ± 0.045

0.751 ± 0.018

4.577 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski