Prosthecochloris sp. GSB1

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Chlorobi; Chlorobia; Chlorobiales; Chlorobiaceae; Prosthecochloris; unclassified Prosthecochloris

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2269 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A222SIX9|A0A222SIX9_9CHLB Uncharacterized protein OS=Prosthecochloris sp. GSB1 OX=281093 GN=CHL67_08285 PE=4 SV=1
MM1 pKa = 7.91RR2 pKa = 11.84KK3 pKa = 9.86NIQHH7 pKa = 6.64KK8 pKa = 10.84LEE10 pKa = 4.84DD11 pKa = 3.48YY12 pKa = 10.5FLRR15 pKa = 11.84EE16 pKa = 3.59RR17 pKa = 11.84YY18 pKa = 9.63LPEE21 pKa = 3.93EE22 pKa = 4.0SQEE25 pKa = 3.96FLKK28 pKa = 11.06DD29 pKa = 3.26SAEE32 pKa = 4.24YY33 pKa = 10.31AVPATDD39 pKa = 3.39SASAGTAIATGYY51 pKa = 8.3KK52 pKa = 9.2TDD54 pKa = 4.0AGNISWASGDD64 pKa = 3.86PEE66 pKa = 4.42NGEE69 pKa = 4.12LTTIAEE75 pKa = 4.51TLRR78 pKa = 11.84SEE80 pKa = 3.95EE81 pKa = 4.0GFAIGVVSTVPFSHH95 pKa = 6.69ATPAVFVSHH104 pKa = 7.18DD105 pKa = 3.56VNRR108 pKa = 11.84GNKK111 pKa = 9.23YY112 pKa = 10.72DD113 pKa = 3.59IAHH116 pKa = 7.3EE117 pKa = 4.25ILFEE121 pKa = 4.11TQPDD125 pKa = 3.92VVIGSGYY132 pKa = 8.68EE133 pKa = 3.54NSYY136 pKa = 10.17FARR139 pKa = 11.84TKK141 pKa = 10.34TVDD144 pKa = 3.39EE145 pKa = 4.93NGNDD149 pKa = 3.55YY150 pKa = 11.45NDD152 pKa = 5.07DD153 pKa = 3.32YY154 pKa = 12.02DD155 pKa = 4.59SFVNGTDD162 pKa = 3.0GTDD165 pKa = 3.34YY166 pKa = 11.44VFVQRR171 pKa = 11.84DD172 pKa = 3.32EE173 pKa = 5.2DD174 pKa = 4.49LDD176 pKa = 4.93GGDD179 pKa = 5.16LLMEE183 pKa = 4.97AANSVDD189 pKa = 3.61LQAGEE194 pKa = 4.38KK195 pKa = 10.69LFGLFGTSGGNFEE208 pKa = 5.16YY209 pKa = 11.14YY210 pKa = 10.41DD211 pKa = 3.64VDD213 pKa = 4.65DD214 pKa = 5.12NPGSPSITRR223 pKa = 11.84DD224 pKa = 3.01GDD226 pKa = 3.64GDD228 pKa = 4.22GVDD231 pKa = 4.33EE232 pKa = 5.69DD233 pKa = 4.19PTLAEE238 pKa = 3.96ATIAALTVLDD248 pKa = 3.74QDD250 pKa = 3.36EE251 pKa = 5.26DD252 pKa = 4.41GFFVMIEE259 pKa = 3.91QGDD262 pKa = 3.91IDD264 pKa = 4.49WNNHH268 pKa = 4.51ANDD271 pKa = 4.04YY272 pKa = 10.79EE273 pKa = 4.29NMIGSVYY280 pKa = 10.98DD281 pKa = 3.59LDD283 pKa = 3.66QAVRR287 pKa = 11.84VVEE290 pKa = 4.37AYY292 pKa = 10.9VDD294 pKa = 3.68IEE296 pKa = 4.71GDD298 pKa = 3.89DD299 pKa = 3.97MDD301 pKa = 3.81WSNTLVIVTSDD312 pKa = 3.02HH313 pKa = 6.5SNSYY317 pKa = 9.37MRR319 pKa = 11.84TNEE322 pKa = 3.68EE323 pKa = 4.41LGIGDD328 pKa = 4.99LPSQEE333 pKa = 3.7GSPYY337 pKa = 11.15DD338 pKa = 3.32FTYY341 pKa = 10.98PNGEE345 pKa = 4.0VTYY348 pKa = 8.57GTTGHH353 pKa = 5.87TNEE356 pKa = 4.89LVTLQARR363 pKa = 11.84GAGAEE368 pKa = 4.09LFEE371 pKa = 5.14EE372 pKa = 5.08YY373 pKa = 10.54AGSWYY378 pKa = 10.13EE379 pKa = 3.89GTSIVDD385 pKa = 3.03NTQIYY390 pKa = 10.96DD391 pKa = 3.51VMLRR395 pKa = 11.84AAQEE399 pKa = 3.97EE400 pKa = 4.96GVEE403 pKa = 4.14HH404 pKa = 6.77VILFIGDD411 pKa = 3.77GMNVEE416 pKa = 4.87HH417 pKa = 6.89EE418 pKa = 4.46MAASRR423 pKa = 11.84YY424 pKa = 10.08LYY426 pKa = 11.08GDD428 pKa = 4.11DD429 pKa = 5.86QSLAWDD435 pKa = 3.35DD436 pKa = 3.54WGTEE440 pKa = 4.19EE441 pKa = 3.91IGGYY445 pKa = 9.97AGYY448 pKa = 10.76SSTWDD453 pKa = 2.8INTYY457 pKa = 9.47NRR459 pKa = 11.84YY460 pKa = 9.29ADD462 pKa = 3.64EE463 pKa = 4.55VGAEE467 pKa = 4.24AYY469 pKa = 10.45HH470 pKa = 6.3PVTFNPLVGYY480 pKa = 9.17NPKK483 pKa = 10.4RR484 pKa = 11.84GGEE487 pKa = 3.98VPYY490 pKa = 10.0PLEE493 pKa = 4.22SDD495 pKa = 3.57SFFGHH500 pKa = 6.76RR501 pKa = 11.84RR502 pKa = 11.84GQTIIGSKK510 pKa = 10.23NDD512 pKa = 3.23EE513 pKa = 4.03QLIGDD518 pKa = 4.06EE519 pKa = 4.15NDD521 pKa = 3.07NRR523 pKa = 11.84ILGLKK528 pKa = 10.01GDD530 pKa = 4.21DD531 pKa = 3.83VLNGGLGADD540 pKa = 4.11RR541 pKa = 11.84LRR543 pKa = 11.84GGNGSDD549 pKa = 3.14TFVYY553 pKa = 10.59EE554 pKa = 4.09SLKK557 pKa = 11.1DD558 pKa = 3.65SLVTPGARR566 pKa = 11.84DD567 pKa = 3.49VITDD571 pKa = 3.96FKK573 pKa = 10.86PGEE576 pKa = 4.05DD577 pKa = 4.58LIDD580 pKa = 4.08LSSLAEE586 pKa = 3.95FDD588 pKa = 4.57GVILGAGDD596 pKa = 3.53HH597 pKa = 6.81FDD599 pKa = 3.89YY600 pKa = 10.65KK601 pKa = 11.27ASGAQLLFDD610 pKa = 4.13SEE612 pKa = 4.84AGILYY617 pKa = 10.71GNVDD621 pKa = 3.65SDD623 pKa = 3.89NYY625 pKa = 11.01SDD627 pKa = 5.14FAIEE631 pKa = 4.67LIGVDD636 pKa = 3.83SLEE639 pKa = 4.63ASDD642 pKa = 5.1FMLVAA647 pKa = 5.15

Molecular weight: 70.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A222SLJ2|A0A222SLJ2_9CHLB FAD:protein FMN transferase OS=Prosthecochloris sp. GSB1 OX=281093 GN=CHL67_04785 PE=3 SV=1
MM1 pKa = 7.38KK2 pKa = 9.6RR3 pKa = 11.84TFQPHH8 pKa = 3.66NRR10 pKa = 11.84KK11 pKa = 9.13RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 8.63HH16 pKa = 3.75GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.16NGRR28 pKa = 11.84RR29 pKa = 11.84VINSRR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84RR40 pKa = 11.84SLTVSSAMGTRR51 pKa = 11.84GTT53 pKa = 3.98

Molecular weight: 6.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2269

0

2269

719611

38

1639

317.1

35.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.88 ± 0.047

1.146 ± 0.022

5.484 ± 0.036

7.027 ± 0.05

4.46 ± 0.034

8.039 ± 0.05

2.046 ± 0.02

5.954 ± 0.042

4.8 ± 0.044

10.059 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.564 ± 0.024

3.302 ± 0.024

4.341 ± 0.036

2.688 ± 0.024

6.757 ± 0.049

6.344 ± 0.035

4.885 ± 0.035

7.262 ± 0.042

1.048 ± 0.02

2.913 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski